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Re-factoring Pseudomonas putida for biosynthesis of vaIue-added polymers from cellulosic waste

Total Cost €


EC-Contrib. €






 FUTURE project word cloud

Explore the words cloud of the FUTURE project. It provides you a very rough idea of what is the project "FUTURE" about.

agricultural    serve    constituent    roadmap    native    lignocellulose    monomeric    wastes    workhorse    kt2440    construct    metabolism    cell    fundamentals    cellulolytic    cheap    yeast    refactoring    waste    enrichment    biosynthesis    designer    viability    aromatic    biofuel    compounds    displayed    bio    hinders    biocatalytic    single    successfully    added    biopolymers    massive    components    bacterial    biological    effort    rational    vac    cellulosic    missing    earth    orchestration    environmental    gram    never    utilize    sugars    solved    bacterium    biotechnological    putida    context    certain    engineering    laboratory    expanding    physiological    model    versatile    pseudomonas    negative    metabolic    saprophytic    desired    recalcitrant    functions    bacteria    pretreatment    cellulosomes    aromatics    skills    substrates    microorganisms    tools    forming    compromising    nanomachines    host    platform    synthetic    corporate    qualities    abundant    enzymatic    biology    displaying    economy    generating    surface    chemicals    organic    engineered    industrial    efficient    valuable    establishing   

Project "FUTURE" data sheet

The following table provides information about the project.


Organization address
address: CALLE SERRANO 117
city: MADRID
postcode: 28006

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Spain [ES]
 Project website
 Total cost 170˙121 €
 EC max contribution 170˙121 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2015
 Funding Scheme MSCA-IF-EF-ST
 Starting year 2016
 Duration (year-month-day) from 2016-10-01   to  2018-09-30


Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 


 Project objective

Lignocellulose is the most abundant organic matter on Earth and important constituent of agricultural and industrial wastes. Lignocellulose-derived monomeric sugars and aromatic compounds can serve as a cheap substrates for biotechnological production of numerous value-added chemicals (VAC). However, a well-defined bacterial platform that could efficiently utilize lignocellulose for biosynthesis of VAC in a single step is still missing. The saprophytic bacterium Pseudomonas putida KT2440, a robust laboratory workhorse with versatile metabolism, has wide potential to utilize lignocellulose-derived sugars and aromatics for VAC formation. But the need for enzymatic pretreatment of the recalcitrant lignocellulose components hinders the development of a cost-effective processes. This challenge could be solved by expanding the biocatalytic functions of P. putida using cellulosomes, efficient enzymatic nanomachines displayed on the surface of certain cellulolytic microorganisms. Synthetic cellulosomes were successfully engineered in biofuel-generating yeast, but never in a Gram-negative bacterium. The applicant will adopt state-of-the-art approaches and tools of synthetic biology, systems biology and metabolic engineering in order to construct P. putida displaying designer cellulosomes and forming valuable biopolymers directly from cellulosic waste. This task requires a rational orchestration of distinct physiological features of the host in order to achieve the desired qualities without compromising cell viability. In this context, the study will also propose a roadmap for massive refactoring and enrichment of native metabolic properties of environmental bacteria. Thus, the project will not only allow the applicant to enhance his research skills, but will also contribute to both understanding fundamentals of model biological systems and corporate effort aimed at establishing FUTURE knowledge-based bio-economy in Europe.


year authors and title journal last update
List of publications.
2018 Pavel Dvořák, Víctor de Lorenzo
Refactoring the upper sugar metabolism of Pseudomonas putida for co-utilization of cellobiose, xylose, and glucose
published pages: 94-108, ISSN: 1096-7176, DOI: 10.1016/j.ymben.2018.05.019
Metabolic Engineering 48 2019-06-13
2017 Pavel Dvořák, Pablo I. Nikel, Jiří Damborský, Víctor de Lorenzo
Bioremediation 3 . 0 : Engineering pollutant-removing bacteria in the times of systemic biology
published pages: 845-866, ISSN: 0734-9750, DOI: 10.1016/j.biotechadv.2017.08.001
Biotechnology Advances 35/7 2019-06-13

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The information about "FUTURE" are provided by the European Opendata Portal: CORDIS opendata.

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