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New enzymatic oxidation/oxyfunctionalization technologies for added value bio-based products

Total Cost €


EC-Contrib. €






 EnzOx2 project word cloud

Explore the words cloud of the EnzOx2 project. It provides you a very rough idea of what is the project "EnzOx2" about.

enzox2    oxidoreductases    components    apis    plastics    institutes    perform    fdca    bottlenecks    economy    platform    peroxygenases    compounds    oxidative    building    oxidases    specialized    ava    copper    radical    pilot    oxidation    plans    biochem    shown    biotechnology    hydroxylation    firmenich    context    enzymes    world    flavours    feedstocks    flagship    centre    biotransformations    ingredients    bioprocess    active    chiral    csic    enzyme    100    aimplas    clea    plastic    lipids    oxyfunctionalization    acids    bio    smes    chemical    unexplored    special    renewable    biorefineries    sufficient    regio    selective    engineered    fragrances    pharmaceutical    sustainable    greener    leaders    added    dresden    thiolate    plant    co    substrate    acid    industrial    block    conversion    biomass    universities    producing    later    steroids    furandicarboxylic    delft    novozymes    flavo    microbial    hydroxymethylfurfural    fatty    technologies    heme    attain    biochemical    expertise    reactions    lipid    self    tools    hmf    validated    stereo    benefit    sugar    optimized    chiracon    terpenes    free    jenabios    pot    efficient    diformylfuran    blocks   

Project "EnzOx2" data sheet

The following table provides information about the project.


Organization address
address: CALLE SERRANO 117
city: MADRID
postcode: 28006

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Spain [ES]
 Project website
 Total cost 5˙000˙000 €
 EC max contribution 3˙000˙000 € (60%)
 Programme 1. H2020-EU.3.2.6. (Bio-based Industries Joint Technology Initiative (BBI-JTI))
 Code Call H2020-BBI-PPP-2015-2-1
 Funding Scheme BBI-RIA
 Starting year 2016
 Duration (year-month-day) from 2016-11-01   to  2019-10-31


Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
3    TECHNISCHE UNIVERSITEIT DELFT NL (DELFT) participant 400˙000.00
4    Chiracon GmbH DE (LUCKENWALDE) participant 250˙000.00
5    JENABIOS GMBH DE (JENA) participant 244˙000.00
8    AVA BIOCHEM BSL AG CH (MUTTENZ) participant 0.00
9    CLEA TECHNOLOGIES BV NL (DELFT) participant 0.00
10    FIRMENICH SA CH (GENEVE) participant 0.00
11    NOVOZYMES A/S DK (BAGSVAERD) participant 0.00


 Project objective

Sustainable production of chemical building blocks and other added value products from plant biomass is required for a bio-based economy. However, the biomass biorefineries should benefit not only from the use of renewable feedstocks but also from greener and more efficient bio-chemical technologies. Previous projects have shown the potential of oxidative enzymes in the production of some added value compounds from biomass components. Of special interest are still unexplored oxidation/oxyfunctionalization reactions (of sugar and lipid compounds) by microbial oxidoreductases, including new (self-sufficient) heme-thiolate peroxygenases. In this context, EnzOx2 plans to develop a 100% biochemical conversion of bio-based 5-hydroxymethylfurfural (HMF) into diformylfuran, a platform chemical, and 2,5-furandicarboxylic acid (FDCA), a plastic building-block. Oxidases (flavo and copper/radical) and peroxygenases will be used to perform the three-step oxidation of HMF to FDCA in a co-substrate and side-product free, one-pot conversion. On the other hand, highly (regio/stereo) selective hydroxylation of plant lipids (such as fatty acids, terpenes and steroids) by peroxygenases will be optimized for cost-effective production of flavours and fragrances (F&F), active pharmaceutical ingredients (APIs) and others. ENZOX2 aims to solve some main bottlenecks in these industrial processes by the use of bio-chemical tools (new/engineered enzymes and optimized biotransformations), to be later validated at the pilot/flagship scale. To attain this objective the consortium includes: i) two world leaders in industrial enzymes (Novozymes) and F&F (Firmenich); ii) two chemical SMEs producing HMF and chiral APIs (AVA-Biochem and Chiracon); iii) two specialized biotechnology SMEs (JenaBios and CLEA); iv) one technology centre in the Plastics sector (AIMPLAS); and v) three CSIC institutes and two universities (Dresden and Delft) with expertise in enzyme reactions and bioprocess implementation.


List of deliverables.
Brochure describing the main results and their potential impact on different industrial sectors Websites, patent fillings, videos etc. 2020-03-24 10:22:50
Dissemination and use of main project results, including publications and patent licensing Documents, reports 2020-03-24 10:22:50
Brochure describing the main aspects of the project, including objectives and consortium Websites, patent fillings, videos etc. 2019-10-30 08:57:13
Set-up of the project web-site including public and private parts Websites, patent fillings, videos etc. 2019-10-30 08:57:13

Take a look to the deliverables list in detail:  detailed list of EnzOx2 deliverables.


year authors and title journal last update
List of publications.
2019 Javier Martin
Evolución dirigida de la peroxigenasa inespecífica de agrocybe aegerita: tolerancia a disolventes orgánicos mediante deriva genética neutral y evolución adaptativa
published pages: , ISSN: , DOI:
2020 Javier Viña-Gonzalez, Angel T. Martinez, Victor Guallar, Miguel Alcalde
Sequential oxidation of 5-hydroxymethylfurfural to furan-2,5-dicarboxylic acid by an evolved aryl-alcohol oxidase
published pages: 140293, ISSN: 1570-9639, DOI: 10.1016/j.bbapap.2019.140293
Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics 1868/1 2020-01-28
2019 Ana Serrano, Eva Calviño, Juan Carro, María I. Sánchez-Ruiz, F. Javier Cañada, Angel T. Martínez
Complete oxidation of hydroxymethylfurfural to furandicarboxylic acid by aryl-alcohol oxidase
published pages: , ISSN: 1754-6834, DOI: 10.1186/s13068-019-1555-z
Biotechnology for Biofuels 12/1 2020-01-28
2019 Javier Viña
Biotechnology platforms for aryl-alcohol oxidases by directed evolution
published pages: , ISSN: , DOI:
2018 Jan Kiebist
Oxyfunktionalisierung pharmazeutisch relevanter Strukturen durch unspezifische Peroxygenasen der Familie I aus Dikarya-Pilzen
published pages: , ISSN: , DOI:
2019 Iván Ayuso-Fernández, Jorge Rencoret, Ana Gutiérrez, Francisco Javier Ruiz-Dueñas, Angel T. Martínez
Peroxidase evolution in white-rot fungi follows wood lignin evolution in plants
published pages: 17900-17905, ISSN: 0027-8424, DOI: 10.1073/pnas.1905040116
Proceedings of the National Academy of Sciences 116/36 2020-01-14
2019 Carmen Aranda
Biotransformations of industrial interest catalyzed by fungal peroxygenases
published pages: , ISSN: , DOI:
2020 Sara Bello, Pedro Méndez-Trelles, Eva Rodil, Gumersindo Feijoo, Maria Teresa Moreira
Towards improving the sustainability of bioplastics: Process modelling and life cycle assessment of two separation routes for 2,5-furandicarboxylic acid
published pages: 116056, ISSN: 1383-5866, DOI: 10.1016/j.seppur.2019.116056
Separation and Purification Technology 233 2020-01-14
2019 Ivan Ayuso
Resurrection of ancestral ligninolytic peroxidases Resurrección de peroxidasas ligninolíticas ancestrales
published pages: , ISSN: , DOI:
2019 Jefferson O. Romero, Elena Fernández-Fueyo, Fabián Avila-Salas, Rodrigo Recabarren, Jans Alzate-Morales, Angel T. Martínez
Binding and Catalytic Mechanisms of Veratryl Alcohol Oxidation by Lignin Peroxidase: A Theoretical and Experimental Study
published pages: 1066-1074, ISSN: 2001-0370, DOI: 10.1016/j.csbj.2019.07.002
Computational and Structural Biotechnology Journal 17 2020-01-14
2018 Sara Bello, Iana Salim, Pedro Méndez-Trelles, Eva Rodil, Gumersindo Feijoo, Maria Teresa Moreira
Environmental sustainability assessment of HMF and FDCA production from lignocellulosic biomass through life cycle assessment (LCA)
published pages: 105-115, ISSN: 0018-3830, DOI: 10.1515/hf-2018-0100
Holzforschung 73/1 2020-01-14
2018 Mercedes Ramirez-Escudero, Patricia Molina-Espeja, Patricia Gomez de Santos, Martin Hofrichter, Julia Sanz-Aparicio, Miguel Alcalde
Structural Insights into the Substrate Promiscuity of a Laboratory-Evolved Peroxygenase
published pages: 3259-3268, ISSN: 1554-8929, DOI: 10.1021/acschembio.8b00500
ACS Chemical Biology 13/12 2019-10-30
2019 Patricia Molina-Espeja, Paloma Santos-Moriano, Eva García-Ruiz, Antonio Ballesteros, Francisco Plou, Miguel Alcalde
Structure-Guided Immobilization of an Evolved Unspecific Peroxygenase
published pages: 1627, ISSN: 1422-0067, DOI: 10.3390/ijms20071627
International Journal of Molecular Sciences 20/7 2019-10-30
2019 Florian Tieves, Sébastien Jean-Paul Willot, Morten Martinus Cornelis Harald van Schie, Marine Charlène Renée Rauch, Sabry Hamdy Hamed Younes, Wuyuan Zhang, JiaJia Dong, Patricia Gomez de Santos, John Mick Robbins, Bettina Bommarius, Miguel Alcalde, Andreas Sebastian Bommarius, Frank Hollmann
Formate Oxidase (FOx) from Aspergillus oryzae : One Catalyst Enables Diverse H 2 O 2 -Dependent Biocatalytic Oxidation Reactions
published pages: 7873-7877, ISSN: 1433-7851, DOI: 10.1002/anie.201902380
Angewandte Chemie International Edition 58/23 2019-10-30
2018 Alexander Karich, Sebastian Kleeberg, René Ullrich, Martin Hofrichter
Enzymatic Preparation of 2,5-Furandicarboxylic Acid (FDCA)—A Substitute of Terephthalic Acid—By the Joined Action of Three Fungal Enzymes
published pages: 5, ISSN: 2076-2607, DOI: 10.3390/microorganisms6010005
Microorganisms 6/1 2019-10-30
2018 Javier Martin-Diaz, Carmen Paret, Eva García-Ruiz, Patricia Molina-Espeja, Miguel Alcalde
Shuffling the neutral drift of unspecific peroxygenase in Saccharomyces cerevisiae
published pages: AEM.00808-18, ISSN: 0099-2240, DOI: 10.1128/AEM.00808-18
Applied and Environmental Microbiology 2019-10-30
2018 Iván Ayuso-Fernández, Francisco J. Ruiz-Dueñas, Angel T. Martínez
Evolutionary convergence in lignin-degrading enzymes
published pages: 201802555, ISSN: 0027-8424, DOI: 10.1073/pnas.1802555115
Proceedings of the National Academy of Sciences 2019-10-30
2017 Enrico Büttner, Anna Maria Gebauer, Martin Hofrichter, Christiane Liers, Harald Kellner
Draft Genome Sequence of the Wood-Degrading Ascomycete Kretzschmaria deusta DSM 104547
published pages: e01076-17, ISSN: 2169-8287, DOI: 10.1128/genomeA.01076-17
Genome Announcements 5/43 2019-10-30
2017 Angel T. Martínez, Francisco J. Ruiz-Dueñas, Susana Camarero, Ana Serrano, Dolores Linde, Henrik Lund, Jesper Vind, Morten Tovborg, Owik M. Herold-Majumdar, Martin Hofrichter, Christiane Liers, René Ullrich, Katrin Scheibner, Giovanni Sannia, Alessandra Piscitelli, Cinzia Pezzella, Mehmet E. Sener, Sibel Kılıç, Willem J.H. van Berkel, Victor Guallar, Maria Fátima Lucas, Ralf Zuhse, Roland Ludwig, Frank Hollmann, Elena Fernández-Fueyo, Eric Record, Craig B. Faulds, Marta Tortajada, Ib Winckelmann, Jo-Anne Rasmussen, Mirjana Gelo-Pujic, Ana Gutiérrez, José C. del Río, Jorge Rencoret, Miguel Alcalde
Oxidoreductases on their way to industrial biotransformations
published pages: 815-831, ISSN: 0734-9750, DOI: 10.1016/j.biotechadv.2017.06.003
Biotechnology Advances 35/6 2019-10-30
2017 Juan Carro, Marta Martínez-Júlvez, Milagros Medina, Angel T. Martínez, Patricia Ferreira
Protein dynamics promote hydride tunnelling in substrate oxidation by aryl-alcohol oxidase
published pages: 28666-28675, ISSN: 1463-9076, DOI: 10.1039/C7CP05904C
Physical Chemistry Chemical Physics 19/42 2019-10-30
2018 Juan Carro, Elena Fernández-Fueyo, Carmen Fernández-Alonso, Javier Cañada, René Ullrich, Martin Hofrichter, Miguel Alcalde, Patricia Ferreira, Angel T. Martínez
Self-sustained enzymatic cascade for the production of 2,5-furandicarboxylic acid from 5-methoxymethylfurfural
published pages: , ISSN: 1754-6834, DOI: 10.1186/s13068-018-1091-2
Biotechnology for Biofuels 11/1 2019-10-30
2018 Carmen Aranda, René Ullrich, Jan Kiebist, Katrin Scheibner, José C. del Río, Martin Hofrichter, Angel T. Martínez, Ana Gutiérrez
Selective synthesis of the resveratrol analogue 4,4′-dihydroxy- trans -stilbene and stilbenoids modification by fungal peroxygenases
published pages: 2394-2401, ISSN: 2044-4753, DOI: 10.1039/c8cy00272j
Catalysis Science & Technology 8/9 2019-10-30
2016 Verónica Sáez-Jiménez, Jorge Rencoret, Miguel Angel Rodríguez-Carvajal, Ana Gutiérrez, Francisco Javier Ruiz-Dueñas, Angel T. Martínez
Role of surface tryptophan for peroxidase oxidation of nonphenolic lignin
published pages: nn, ISSN: 1754-6834, DOI: 10.1186/s13068-016-0615-x
Biotechnology for Biofuels 9/1 2019-10-30
2017 David Rodríguez-Escribano, Felipe de Salas, Isabel Pardo, Susana Camarero
High-Throughput Screening Assay for Laccase Engineering toward Lignosulfonate Valorization
published pages: 1793, ISSN: 1422-0067, DOI: 10.3390/ijms18081793
International Journal of Molecular Sciences 18/8 2019-10-30
2017 Sandra Acebes, Francisco J. Ruiz-Dueñas, Mario Toubes, Veronica Sáez-Jiménez, Marta Pérez-Boada, M. Fátima Lucas, Angel T. Martínez, Victor Guallar
Mapping the Long-Range Electron Transfer Route in Ligninolytic Peroxidases
published pages: 3946-3954, ISSN: 1520-6106, DOI: 10.1021/acs.jpcb.7b00835
The Journal of Physical Chemistry B 121/16 2019-10-30
2017 Jan Kiebist, Kai-Uwe Schmidtke, Jörg Zimmermann, Harald Kellner, Nico Jehmlich, René Ullrich, Daniel Zänder, Martin Hofrichter, Katrin Scheibner
A peroxygenase from Chaetomium globosum catalyzes the selective oxygenation of testosterone
published pages: , ISSN: 1439-4227, DOI: 10.1002/cbic.201600677
ChemBioChem 2019-10-30
2018 Juan Carro, Patricia Ferreira, Angel T. Martínez, Giovanni Gadda
Stepwise Hydrogen Atom and Proton Transfers in Dioxygen Reduction by Aryl-Alcohol Oxidase
published pages: 1790-1797, ISSN: 0006-2960, DOI: 10.1021/acs.biochem.8b00106
Biochemistry 57/11 2019-10-30
2017 Alexander Karich, René Ullrich, Katrin Scheibner, Martin Hofrichter
Fungal Unspecific Peroxygenases Oxidize the Majority of Organic EPA Priority Pollutants
published pages: , ISSN: 1664-302X, DOI: 10.3389/fmicb.2017.01463
Frontiers in Microbiology 8 2019-10-30
2018 Patricia Gomez de Santos, Marina Cañellas, Florian Tieves, Sabry H. H. Younes, Patricia Molina-Espeja, Martin Hofrichter, Frank Hollmann, Victor Guallar, Miguel Alcalde
Selective Synthesis of the Human Drug Metabolite 5′-Hydroxypropranolol by an Evolved Self-Sufficient Peroxygenase
published pages: 4789-4799, ISSN: 2155-5435, DOI: 10.1021/acscatal.8b01004
ACS Catalysis 2019-10-30
2017 Andrés Olmedo, José C. del Río, Jan Kiebist, René Ullrich, Martin Hofrichter, Katrin Scheibner, Angel T. Martínez, Ana Gutiérrez
Fatty Acid Chain Shortening by a Fungal Peroxygenase
published pages: 16985-16989, ISSN: 0947-6539, DOI: 10.1002/chem.201704773
Chemistry - A European Journal 23/67 2019-10-30
2018 Juan Carro, Pep Amengual-Rigo, Ferran Sancho, Milagros Medina, Victor Guallar, Patricia Ferreira, Angel T. Martínez
Multiple implications of an active site phenylalanine in the catalysis of aryl-alcohol oxidase
published pages: , ISSN: 2045-2322, DOI: 10.1038/s41598-018-26445-x
Scientific Reports 8/1 2019-10-30
2018 René Ullrich, Marzena Poraj-Kobielska, Steffi Scholze, Claire Halbout, Martin Sandvoss, Marek J. Pecyna, Katrin Scheibner, Martin Hofrichter
Side chain removal from corticosteroids by unspecific peroxygenase
published pages: 84-93, ISSN: 0162-0134, DOI: 10.1016/j.jinorgbio.2018.03.011
Journal of Inorganic Biochemistry 183 2019-10-30
2017 Ivan Mateljak, Thierry Tron, Miguel Alcalde
Evolved α-factor prepro-leaders for directed laccase evolution in Saccharomyces cerevisiae
published pages: 1830-1836, ISSN: 1751-7915, DOI: 10.1111/1751-7915.12838
Microbial Biotechnology 10/6 2019-10-30
2018 Sabrina Leonhardt, Enrico Büttner, Anna Maria Gebauer, Martin Hofrichter, Harald Kellner
Draft Genome Sequence of the Sordariomycete Lecythophora ( Coniochaeta ) hoffmannii CBS 245.38
published pages: e01510-17, ISSN: 2169-8287, DOI: 10.1128/genomeA.01510-17
Genome Announcements 6/7 2019-10-30
2017 Iván Ayuso-Fernández, Angel T. Martínez, Francisco J. Ruiz-Dueñas
Experimental recreation of the evolution of lignin-degrading enzymes from the Jurassic to date
published pages: , ISSN: 1754-6834, DOI: 10.1186/s13068-017-0744-x
Biotechnology for Biofuels 10/1 2019-10-30
2019 Iván Ayuso-Fernández, Antonio L. De Lacey, Francisco J. Cañada, Francisco J. Ruiz-Dueñas, Angel T. Martínez
Increase of Redox Potential during the Evolution of Enzymes Degrading Recalcitrant Lignin
published pages: 2708-2712, ISSN: 0947-6539, DOI: 10.1002/chem.201805679
Chemistry - A European Journal 25/11 2019-10-30
2018 JiaJia Dong, Elena Fernández-Fueyo, Frank Hollmann, Caroline E. Paul, Milja Pesic, Sandy Schmidt, Yonghua Wang, Sabry Younes, Wuyuan Zhang
Biocatalytic Oxidation Reactions: A Chemist\'s Perspective
published pages: 9238-9261, ISSN: 1433-7851, DOI: 10.1002/anie.201800343
Angewandte Chemie International Edition 57/30 2019-10-30
2019 Florian Tieves, Fabio Tonin, Elena Fernández-Fueyo, John M. Robbins, Bettina Bommarius, Andreas S. Bommarius, Miguel Alcalde, Frank Hollmann
Energising the E-factor: The E+-factor
published pages: 1311-1314, ISSN: 0040-4020, DOI: 10.1016/j.tet.2019.01.065
Tetrahedron 75/10 2019-10-30
2019 Carmen Aranda, Martí Municoy, Víctor Guallar, Jan Kiebist, Katrin Scheibner, René Ullrich, José C. del Río, Martin Hofrichter, Angel T. Martínez, Ana Gutiérrez
Selective synthesis of 4-hydroxyisophorone and 4-ketoisophorone by fungal peroxygenases
published pages: , ISSN: 2044-4753, DOI: 10.1039/c8cy02114g
Catalysis Science & Technology 2019-10-30
2017 Juan R. Carro Aramburu
Nuevas oxidorreductasas GMC de basidiomicetos ligninolíticos: Screening genómico, mecanismo catalítico y potencial biotecnológico
published pages: , ISSN: , DOI:
2018 Olmedo, Andrés
Nuevas reacciones de interés industrial con peroxigenasas fúngicas
published pages: , ISSN: , DOI:
2018 Elena Fernández-Fueyo, Irene Davó-Siguero, David Almendral, Dolores Linde, Maria Camilla Baratto, Rebecca Pogni, Antonio Romero, Victor Guallar, Angel T. Martínez
Description of a Non-Canonical Mn(II)-Oxidation Site in Peroxidases
published pages: 8386-8395, ISSN: 2155-5435, DOI: 10.1021/acscatal.8b02306
ACS Catalysis 8/9 2019-10-30
2017 Diana M. Mate, Miguel A. Palomino, Patricia Molina-Espeja, Javier Martin-Diaz, Miguel Alcalde
Modification of the peroxygenative:peroxidative activity ratio in the unspecific peroxygenase from Agrocybe aegerita by structure-guided evolution
published pages: , ISSN: 1741-0126, DOI: 10.1093/protein/gzw073
Protein Engineering Design and Selection 2019-10-30

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