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ivMX

Development of the new generation of structural biology by coupling in vivo crystallography to intense x-ray sources

Total Cost €

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EC-Contrib. €

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Partnership

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 ivMX project word cloud

Explore the words cloud of the ivMX project. It provides you a very rough idea of what is the project "ivMX" about.

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Project "ivMX" data sheet

The following table provides information about the project.

Coordinator
SYNCHROTRON SOLEIL SOCIETE CIVILE 

Organization address
address: L ORME DES MERISIERS
city: SAINT AUBIN
postcode: 91190
website: http://www.synchrotron-soleil.fr/

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country France [FR]
 Project website https://www.synchrotron-soleil.fr/fr/lignes-de-lumiere/proxima-1
 Total cost 185˙076 €
 EC max contribution 185˙076 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2015
 Funding Scheme MSCA-IF-EF-ST
 Starting year 2016
 Duration (year-month-day) from 2016-09-01   to  2018-08-31

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    SYNCHROTRON SOLEIL SOCIETE CIVILE FR (SAINT AUBIN) coordinator 185˙076.00

Map

 Project objective

Mostly motivated by, but not restricted to the comprehensive analysis and understanding of biological phenomena with potential medical implications, structural biology gives access to the atomic details of macromolecules, allowing to decipher their biological functions. Using state-of-the-art developments coupled to third-generation x-ray synchrotron sources, macromolecular x-ray crystallography (MX) stands as the primary method for determining the structures of proteins, alone or in complex with partner ligands. The major bottleneck of MX lies in the requirement to obtain crystals of reasonable sizes that can easily by handled and readily give rise to interpretable diffraction patterns. Prior to x-ray diffraction studies, crystal production pipelines involve the characterisation, purification and handling of samples in large quantities through multi-step, complicated, time-consuming and costly procedures.

The identification of protein crystals naturally occurring inside cells and organisms as diverse as bacteria, protists, fungi, plants, fishes, amphibians, insects and mammals has opened a window for a new type of MX and structural biology. Recently, the emergence of the in vivo crystallography (ivMX) approach took advantage in the developments of new intense coherent x-ray sources that allow collecting diffraction patterns from sub-micron crystals, targets so far unreachable at other x-ray sources. This proposal intends to get further insights into the yet uncontrollable events dictating in vivo crystal growth, by structure determination and analysis of readily available ivMX systems. While deciphering these phenomena and applying them to external proteins recombinantly expressed in hosts where in vivo crystal growth could be identified, a small platform for ivMX will be initiated, with the aim of reducing the tedious and costly sample preparation steps currently used in MX.

 Publications

year authors and title journal last update
List of publications.
2017 Pierre Montaville, Leonard Chavas
De novo in-vivo protein crystal structure: is experimental phasing required?
published pages: C878, ISSN: , DOI:
Acta Cryst A73 2019-05-27
2017 Charline J. J. Gerard, Gilles Ferry, Laurent M. Vuillard, Jean A. Boutin, Leonard M. G. Chavas, Tiphaine Huet, Nathalie Ferte, Romain Grossier, Nadine Candoni, Stéphane Veesler
Crystallization via tubing microfluidics permits both in situ and ex situ X-ray diffraction
published pages: 574-578, ISSN: 2053-230X, DOI: 10.1107/S2053230X17013826
Acta Crystallographica Section F Structural Biology Communications 73/10 2019-05-27
2018 Sanchari Banerjee, Pierre Montaville, Leonard M. G. Chavas, S. Ramaswamy
The New Era of Microcrystallography
published pages: 273-281, ISSN: 0019-4964, DOI: 10.1007/s41745-018-0086-0
Journal of the Indian Institute of Science 98/3 2019-05-27

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