Explore the words cloud of the HBVssNMR project. It provides you a very rough idea of what is the project "HBVssNMR" about.
The following table provides information about the project.
CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE CNRS
|Coordinator Country||France [FR]|
|Total cost||185˙076 €|
|EC max contribution||143˙603 € (78%)|
1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
|Duration (year-month-day)||from 2017-06-01 to 2019-05-31|
Take a look of project's partnership.
|1||CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE CNRS||FR (PARIS)||coordinator||143˙603.00|
We will seek in this project to reveal structural and dynamic features of the C-terminal domain (CTD) of the Hepatitis B virus (HBV) core protein which encapsidates the viral genome. Each year, over 750,000 deaths are due to serious liver diseases caused by chronic HBV infection. Hepatitis B is, despite the availability of a vaccine, still a major health problem. The HBV virus particle is composed of an outer lipid-protein envelope and a core protein of 20 kDa that self-assembles into icosahedral nucleocapsids. The nucleocapsid encloses the pregenomic RNA (pgRNA) that is then reverse-transcribed into DNA by the viral polymerase (Pol). During pgRNA encapsidation, the CTD is thought to interact with the RNA. Despite the essential role played by the CTD in the regulation of the viral life cycle through various conformational states and phosphorylation patterns, it escapes today to classical structural biology approaches due to its dynamic nature. We will use solid-state NMR to bring new insights into the precise conformation and localization of the CTD along various steps of the viral life cycle. Sample preparation protocols of the core protein have recently been established by the applicant in the host laboratory using bacterial expression systems. The first NMR spectra of the nucleocapsid look highly promising. Further experiments will be recorded to assign the resonances and identify the CTD signals. Different phosphorylation states and binding partners, as the pgRNA, will be explored. Finally, paramagnetic tags will be used to obtain information about the positioning of the CTD. Our work shall contribute to expand the present molecular description of the HBV life cycle to include the role of the CTD. Our results will help to understand, and ultimately to target, its central role in HBV infection.
|year||authors and title||journal||last update|
Lauriane Lecoq, Shishan Wang, Thomas Wiegand, StÃ©phane Bressanelli, Michael Nassal, Beat H. Meier, Anja BÃ¶ckmann
Solid-state [13Câ€“15N] NMR resonance assignment of hepatitis B virus core protein
published pages: , ISSN: 1874-2718, DOI: 10.1007/s12104-018-9810-y
|Biomolecular NMR Assignments||2019-10-29|
Are you the coordinator (or a participant) of this project? Plaese send me more information about the "HBVSSNMR" project.
For instance: the website url (it has not provided by EU-opendata yet), the logo, a more detailed description of the project (in plain text as a rtf file or a word file), some pictures (as picture files, not embedded into any word file), twitter account, linkedin page, etc.
Send me an email (email@example.com) and I put them in your project's page as son as possible.
Thanks. And then put a link of this page into your project's website.
The information about "HBVSSNMR" are provided by the European Opendata Portal: CORDIS opendata.
Natural Product-Inspired Therapies for Leishmaniasis and Chagas DiseaseRead More
General readout electronics for cross-sectoral application in European research infrastructureRead More
Narrative, Writing, and the Teotihuacan Language: Exploring Language History Through Phylogenetics, Epigraphy and IconographyRead More