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Rep. Fork Restart

Restart of blocked replication fork at replication fork barrier

Total Cost €

0

EC-Contrib. €

0

Partnership

0

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 Rep. Fork Restart project word cloud

Explore the words cloud of the Rep. Fork Restart project. It provides you a very rough idea of what is the project "Rep. Fork Restart" about.

investigator    actors    fellowship    hr    barriers    chip    obstacles    bacteria    2d    collapsed    gels    electron    complete    copy    inherited    fork    data    muscular    molecular    sequences    replication    nature    bypass    prokaryotes    mutation    slippage    blocked    contacts    rescued    diseases    recombination    acquired    rna    characterised    myself    structures    experts    microscopy    dna    restarted    lesions    rfbs    rfb    rfs    serious    bound    characterise    cells    training    rearrangements    yeast    challenged    formed    stalled    structure    fission    gain    single    hybrids    homologous    mechanism    rts1    successfully    rf    techniques    doctoral    chromosomal    converging    cancer    fails    host    proteins    constantly    understand    career    newly    errors    gross    forks    eukaryotic    unravel    laboratory    prone    site    forming    transit    variations    recruitment    neurological    questions    mentoring    lab    error    independent    restart    me   

Project "Rep. Fork Restart" data sheet

The following table provides information about the project.

Coordinator
THE UNIVERSITY OF SUSSEX 

Organization address
address: SUSSEX HOUSE FALMER
city: BRIGHTON
postcode: BN1 9RH
website: http://www.sussex.ac.uk

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country United Kingdom [UK]
 Project website http://www.address.com
 Total cost 183˙454 €
 EC max contribution 183˙454 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2016
 Funding Scheme MSCA-IF-EF-ST
 Starting year 2017
 Duration (year-month-day) from 2017-05-01   to  2019-04-30

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    THE UNIVERSITY OF SUSSEX UK (BRIGHTON) coordinator 183˙454.00

Map

 Project objective

Errors in replication of DNA lead to serious diseases like cancer, inherited neurological and muscular diseases. Replication is constantly challenged by various obstacles that can result in error-prone replication. Replication fork barriers (RFBs) include proteins bound to DNA, structure forming sequences and DNA/RNA hybrids. Replication forks (RFs) stalled or blocked at these barriers can be rescued by a converging fork. But if this fails blocked RF must be restarted to complete replication. Restart using homologous recombination (HR) can lead to gross chromosomal rearrangements and copy number variations. The newly restarted replication fork is also prone to slippage leading to mutation and rearrangements. The research questions I will address are how this RF is restarted and why it is error prone. I will focus on restart at a site-specific RFB in fission yeast, RTS1. Restart at RTS1 has been well characterised in the host laboratory. My project will identify DNA structures formed during the RF restart and characterise recruitment of factors involved in the restart of the collapsed RF. The acquired data will enable us to further understand the mechanism of RF restart and to unravel the cause of the error prone nature of newly restarted RF. I consider this fellowship a key step in my career as it allows me to transit from the analysis of site-specific lesions in prokaryotes (previous doctoral fellowship) to eukaryotic cells, gain experience in a wide range of techniques (2D gels, Electron microscopy, ChIP) and build contacts with experts in the field of eukaryotic replication restart. This is necessary for my future independent research and will enable me to set up my lab to study molecular actors and DNA structures during bypass of single lesions in bacteria and eukaryotic cells. The mentoring during the fellowship will provide me with the necessary training to successfully establish myself as an independent investigator.

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The information about "REP. FORK RESTART" are provided by the European Opendata Portal: CORDIS opendata.

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