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Rep. Fork Restart

Restart of blocked replication fork at replication fork barrier

Total Cost €

0

EC-Contrib. €

0

Partnership

0

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 Rep. Fork Restart project word cloud

Explore the words cloud of the Rep. Fork Restart project. It provides you a very rough idea of what is the project "Rep. Fork Restart" about.

formed    replication    yeast    gross    muscular    rfbs    inherited    lesions    complete    challenged    rescued    neurological    2d    rfs    experts    molecular    recruitment    single    copy    barriers    laboratory    rearrangements    host    fork    proteins    homologous    stalled    converging    forks    unravel    error    constantly    chromosomal    newly    mentoring    me    lab    serious    hybrids    techniques    myself    electron    microscopy    recombination    collapsed    blocked    diseases    mechanism    forming    bypass    acquired    hr    errors    prone    training    actors    sequences    site    characterised    fission    doctoral    obstacles    characterise    gain    structure    rfb    rna    variations    structures    rts1    restart    eukaryotic    questions    contacts    restarted    successfully    cancer    understand    independent    rf    fails    chip    transit    dna    slippage    bacteria    fellowship    career    mutation    gels    cells    bound    investigator    nature    data    prokaryotes   

Project "Rep. Fork Restart" data sheet

The following table provides information about the project.

Coordinator
THE UNIVERSITY OF SUSSEX 

Organization address
address: SUSSEX HOUSE FALMER
city: BRIGHTON
postcode: BN1 9RH
website: http://www.sussex.ac.uk

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country United Kingdom [UK]
 Project website http://www.address.com
 Total cost 183˙454 €
 EC max contribution 183˙454 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2016
 Funding Scheme MSCA-IF-EF-ST
 Starting year 2017
 Duration (year-month-day) from 2017-05-01   to  2019-04-30

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    THE UNIVERSITY OF SUSSEX UK (BRIGHTON) coordinator 183˙454.00

Map

 Project objective

Errors in replication of DNA lead to serious diseases like cancer, inherited neurological and muscular diseases. Replication is constantly challenged by various obstacles that can result in error-prone replication. Replication fork barriers (RFBs) include proteins bound to DNA, structure forming sequences and DNA/RNA hybrids. Replication forks (RFs) stalled or blocked at these barriers can be rescued by a converging fork. But if this fails blocked RF must be restarted to complete replication. Restart using homologous recombination (HR) can lead to gross chromosomal rearrangements and copy number variations. The newly restarted replication fork is also prone to slippage leading to mutation and rearrangements. The research questions I will address are how this RF is restarted and why it is error prone. I will focus on restart at a site-specific RFB in fission yeast, RTS1. Restart at RTS1 has been well characterised in the host laboratory. My project will identify DNA structures formed during the RF restart and characterise recruitment of factors involved in the restart of the collapsed RF. The acquired data will enable us to further understand the mechanism of RF restart and to unravel the cause of the error prone nature of newly restarted RF. I consider this fellowship a key step in my career as it allows me to transit from the analysis of site-specific lesions in prokaryotes (previous doctoral fellowship) to eukaryotic cells, gain experience in a wide range of techniques (2D gels, Electron microscopy, ChIP) and build contacts with experts in the field of eukaryotic replication restart. This is necessary for my future independent research and will enable me to set up my lab to study molecular actors and DNA structures during bypass of single lesions in bacteria and eukaryotic cells. The mentoring during the fellowship will provide me with the necessary training to successfully establish myself as an independent investigator.

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The information about "REP. FORK RESTART" are provided by the European Opendata Portal: CORDIS opendata.

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