Opendata, web and dolomites


Dissecting the Function of Multiple Polycomb Group Complexes in Establishing Transcriptional Identity

Total Cost €


EC-Contrib. €






 DissectPcG project word cloud

Explore the words cloud of the DissectPcG project. It provides you a very rough idea of what is the project "DissectPcG" about.

lysine    differentiation    mutations    renewal    119    prc2    regulating    functions    pcg    composition    homeostasis    ubiquitination    systematically    h2a    unclear    links    pcgs    catalyses    chromatin    h3    adult    cancer    forms    histone    core    advantage    variety    contribution    underlying    expression    fundamental    genetic    phenotypes    manner    classified    gain    tissue    maintenance    contributions    biochemical    correct    cell    retains    published    mechanisms    exist    subunits    altered    identity    co    newly    either    transcriptional    heterogeneous    mutually    group    relative    programs    tissues    tri    critically    function    share    their    tumours    pcgf    pcgf1    line    largely    prc1    exclusive    stem    gene    six    cellular    unpublished    complexes    biological    elucidate    heterogeneity    mouse    cells    dissects    polycomb    embryonic    methylation    assembling    mechanistic    molecular    self    repressive    models    modifiers    reagents    establishment    deregulation    unknown    genes    27    subunit    dissect    differ    profiles    catalytic   

Project "DissectPcG" data sheet

The following table provides information about the project.


Organization address
address: Via Festa Del Perdono 7
city: MILANO
postcode: 20122

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Italy [IT]
 Total cost 2˙000˙000 €
 EC max contribution 2˙000˙000 € (100%)
 Programme 1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC))
 Code Call ERC-2016-COG
 Funding Scheme ERC-COG
 Starting year 2017
 Duration (year-month-day) from 2017-11-01   to  2022-10-31


Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    UNIVERSITA DEGLI STUDI DI MILANO IT (MILANO) coordinator 829˙932.00
2    ISTITUTO EUROPEO DI ONCOLOGIA SRL IT (MILANO) participant 1˙170˙067.00


 Project objective

The activities of the Polycomb group (PcG) of repressive chromatin modifiers are required to maintain correct transcriptional identity during development and differentiation. These activities are altered in a variety of tumours by gain- or loss-of-function mutations, whose mechanistic aspects still remain unclear.

PcGs can be classified in two major repressive complexes (PRC1 and PRC2) with common pathways but distinct biochemical activities. PRC1 catalyses histone H2A ubiquitination of lysine 119, and PRC2 tri-methylation of histone H3 lysine 27. However, PRC1 has a more heterogeneous composition than PRC2, with six mutually exclusive PCGF subunits (PCGF1–6) essential for assembling distinct PRC1 complexes that differ in subunit composition but share the same catalytic core.

While up to six different PRC1 forms can co-exist in a given cell, the molecular mechanisms regulating their activities and their relative contributions to general PRC1 function in any tissue/cell type remain largely unknown. In line with this biochemical heterogeneity, PRC1 retains broader biological functions than PRC2. Critically, however, no molecular analysis has yet been published that dissects the contribution of each PRC1 complex in regulating transcriptional identity.

We will take advantage of newly developed reagents and unpublished genetic models to target each of the six Pcgf genes in either embryonic stem cells or mouse adult tissues. This will systematically dissect the contributions of the different PRC1 complexes to chromatin profiles, gene expression programs, and cellular phenotypes during stem cell self-renewal, differentiation and adult tissue homeostasis. Overall, this will elucidate some of the fundamental mechanisms underlying the establishment and maintenance of cellular identity and will allow us to further determine the molecular links between PcG deregulation and cancer development in a tissue- and/or cell type–specific manner.


year authors and title journal last update
List of publications.
2019 Elisa Lavarone, Caterina M. Barbieri, Diego Pasini
Dissecting the role of H3K27 acetylation and methylation in PRC2 mediated control of cellular identity
published pages: , ISSN: 2041-1723, DOI: 10.1038/s41467-019-09624-w
Nature Communications 10/1 2019-06-27
2019 Silvia Pivetti, Daniel Fernandez-Perez, Alessandro D’Ambrosio, Caterina Maria Barbieri, Daria Manganaro, Alessandra Rossi, Laura Barnabei, Marika Zanotti, Andrea Scelfo, Fulvio Chiacchiera, Diego Pasini
Loss of PRC1 activity in different stem cell compartments activates a common transcriptional program with cell type–dependent outcomes
published pages: eaav1594, ISSN: 2375-2548, DOI: 10.1126/sciadv.aav1594
Science Advances 5/5 2019-08-05
2019 Andrea Scelfo, Daniel Fernández-Pérez, Simone Tamburri, Marika Zanotti, Elisa Lavarone, Monica Soldi, Tiziana Bonaldi, Karin Johanna Ferrari, Diego Pasini
Functional Landscape of PCGF Proteins Reveals Both RING1A/B-Dependent-and RING1A/B-Independent-Specific Activities
published pages: 1037-1052.e7, ISSN: 1097-2765, DOI: 10.1016/j.molcel.2019.04.002
Molecular Cell 74/5 2019-06-27

Are you the coordinator (or a participant) of this project? Plaese send me more information about the "DISSECTPCG" project.

For instance: the website url (it has not provided by EU-opendata yet), the logo, a more detailed description of the project (in plain text as a rtf file or a word file), some pictures (as picture files, not embedded into any word file), twitter account, linkedin page, etc.

Send me an  email ( and I put them in your project's page as son as possible.

Thanks. And then put a link of this page into your project's website.

The information about "DISSECTPCG" are provided by the European Opendata Portal: CORDIS opendata.

More projects from the same programme (H2020-EU.1.1.)

DINAMIX (2019)

Real-time diffusion NMR analysis of mixtures

Read More  

EAST (2020)

Using Evolutionary Algorithms to Understand and Secure Web/Enterprise Systems

Read More  

RUTTER (2019)

Making the Earth Global: Early Modern Nautical Rutters and the Construction of a Global Concept of the Earth

Read More