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DiPaC_MC

Direct Pathway Cloning of Neglected Bacteria in the Hunt for Novel (Bio-)Chemistry

Total Cost €

0

EC-Contrib. €

0

Partnership

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 DiPaC_MC project word cloud

Explore the words cloud of the DiPaC_MC project. It provides you a very rough idea of what is the project "DiPaC_MC" about.

technische    biosynthesis    sources    vital    express    harbor    uuml    fast    unexplored    pcr    genome    enzymes    auml    ern    alteration    outcomes    microbial    mining    hrungs    revealed    gene    engineering    source    direct    constructed    clusters    discover    completely    uncharacterized    und    natural    expression    synthesis    metabolic    biology    biomedical    diversity    loci    revolutionise    lebensmittelforschung    host    assembly    provides    amplicon    bio    techniques       incorporation    poorly    discovering    zentralinstitut    rare    health    gibson    characterisation    immensely    combination    organisms    unusual    chemical    threats    20kb    biocatalytic    genetic    discovery    resurgence    universit    synthetic    global    vectors    identification    bioactive    downstream    chemistry    regards    potentially    resistance    isolates    antibiotic    ziel    coupled    nchen    toolkit    heterologous    heterologously    overcome    prolific    structural    methodology    cloning    bacteria    bacterial    biochemistry    utilise   

Project "DiPaC_MC" data sheet

The following table provides information about the project.

Coordinator
TECHNISCHE UNIVERSITAET MUENCHEN 

Organization address
address: Arcisstrasse 21
city: MUENCHEN
postcode: 80333
website: www.tu-muenchen.de

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Germany [DE]
 Total cost 159˙460 €
 EC max contribution 159˙460 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2016
 Funding Scheme MSCA-IF-EF-ST
 Starting year 2017
 Duration (year-month-day) from 2017-09-01   to  2019-08-31

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    TECHNISCHE UNIVERSITAET MUENCHEN DE (MUENCHEN) coordinator 159˙460.00

Map

 Project objective

To overcome recent global health threats, such as antibiotic resistance, a resurgence in the discovery of new chemical and thus biomedical diversity from microbial sources is needed. Genome mining in combination with heterologous expression is an approach that will overcome these challenges. It involves incorporation of yet uncharacterized natural product genetic loci into a fast growing heterologous host. For this approach, a prolific source of novel natural product gene clusters is vital. Our collaboration with the Zentralinstitut für Ernährungs- und Lebensmittelforschung (ZIEL, Technische Universität München) provides direct access to bacterial isolates completely unexplored in regards to their potential for natural product biosynthesis. Our genome analyses have revealed that many of the ZIEL isolates harbor a large number of uncharacterized natural product gene clusters. Thus, the aim of this proposal is to heterologously express natural product gene clusters from these neglected bacteria to discover novel natural product (bio-)chemistry. Because these organisms are poorly studied, the likelihood of discovering rare or novel biochemistry is immensely increased. Here, we will utilise a novel combination of synthetic biology techniques referred to as Direct Pathway Cloning. This will enable expression vectors to be constructed by large-amplicon PCR (up to 20kb) coupled to Gibson assembly. Development of the methodology is set to revolutionise synthetic biology and metabolic engineering. Downstream outcomes of this proposal will be the identification of novel, potentially bioactive natural products, the characterisation of unusual biochemistry and the addition of enzymes to the ‘biocatalytic toolkit’ for natural product synthesis and structural alteration.

 Publications

year authors and title journal last update
List of publications.
2020 Marija Mojicevic, Paul M. D\'Agostino, Aleksandar Pavic, Sandra Vojnovic, Ramsankar Senthamaraikannan, Branka Vasiljevic, Tobias A. M. Gulder, Jasmina Nikodinovic‐Runic
Streptomyces sp. BV410 isolate from chamomile rhizosphere soil efficiently produces staurosporine with antifungal and antiangiogenic properties
published pages: , ISSN: 2045-8827, DOI: 10.1002/mbo3.986
MicrobiologyOpen 9/3 2020-04-15
2019 Elke R. Duell, Paul M. D’Agostino, Nicole Shapiro, Tanja Woyke, Thilo M. Fuchs, Tobias A. M. Gulder
Direct pathway cloning of the sodorifen biosynthetic gene cluster and recombinant generation of its product in E. coli
published pages: , ISSN: 1475-2859, DOI: 10.1186/s12934-019-1080-6
Microbial Cell Factories 18/1 2020-01-27
2018 Paul M. D’Agostino, Tobias A. M. Gulder
Direct Pathway Cloning Combined with Sequence- and Ligation-Independent Cloning for Fast Biosynthetic Gene Cluster Refactoring and Heterologous Expression
published pages: 1702-1708, ISSN: 2161-5063, DOI: 10.1021/acssynbio.8b00151
ACS Synthetic Biology 7/7 2020-01-27

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