Explore the words cloud of the DiPaC_MC project. It provides you a very rough idea of what is the project "DiPaC_MC" about.
The following table provides information about the project.
TECHNISCHE UNIVERSITAET MUENCHEN
|Coordinator Country||Germany [DE]|
|Total cost||159˙460 €|
|EC max contribution||159˙460 € (100%)|
1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
|Duration (year-month-day)||from 2017-09-01 to 2019-08-31|
Take a look of project's partnership.
|1||TECHNISCHE UNIVERSITAET MUENCHEN||DE (MUENCHEN)||coordinator||159˙460.00|
To overcome recent global health threats, such as antibiotic resistance, a resurgence in the discovery of new chemical and thus biomedical diversity from microbial sources is needed. Genome mining in combination with heterologous expression is an approach that will overcome these challenges. It involves incorporation of yet uncharacterized natural product genetic loci into a fast growing heterologous host. For this approach, a prolific source of novel natural product gene clusters is vital. Our collaboration with the Zentralinstitut für Ernährungs- und Lebensmittelforschung (ZIEL, Technische Universität München) provides direct access to bacterial isolates completely unexplored in regards to their potential for natural product biosynthesis. Our genome analyses have revealed that many of the ZIEL isolates harbor a large number of uncharacterized natural product gene clusters. Thus, the aim of this proposal is to heterologously express natural product gene clusters from these neglected bacteria to discover novel natural product (bio-)chemistry. Because these organisms are poorly studied, the likelihood of discovering rare or novel biochemistry is immensely increased. Here, we will utilise a novel combination of synthetic biology techniques referred to as Direct Pathway Cloning. This will enable expression vectors to be constructed by large-amplicon PCR (up to 20kb) coupled to Gibson assembly. Development of the methodology is set to revolutionise synthetic biology and metabolic engineering. Downstream outcomes of this proposal will be the identification of novel, potentially bioactive natural products, the characterisation of unusual biochemistry and the addition of enzymes to the ‘biocatalytic toolkit’ for natural product synthesis and structural alteration.
|year||authors and title||journal||last update|
Marija Mojicevic, Paul M. D\'Agostino, Aleksandar Pavic, Sandra Vojnovic, Ramsankar Senthamaraikannan, Branka Vasiljevic, Tobias A. M. Gulder, Jasmina Nikodinovicâ€Runic
Streptomyces sp. BV410 isolate from chamomile rhizosphere soil efficiently produces staurosporine with antifungal and antiangiogenic properties
published pages: , ISSN: 2045-8827, DOI: 10.1002/mbo3.986
Elke R. Duell, Paul M. Dâ€™Agostino, Nicole Shapiro, Tanja Woyke, Thilo M. Fuchs, Tobias A. M. Gulder
Direct pathway cloning of the sodorifen biosynthetic gene cluster and recombinant generation of its product in E. coli
published pages: , ISSN: 1475-2859, DOI: 10.1186/s12934-019-1080-6
|Microbial Cell Factories 18/1||2020-01-27|
Paul M. Dâ€™Agostino, Tobias A. M. Gulder
Direct Pathway Cloning Combined with Sequence- and Ligation-Independent Cloning for Fast Biosynthetic Gene Cluster Refactoring and Heterologous Expression
published pages: 1702-1708, ISSN: 2161-5063, DOI: 10.1021/acssynbio.8b00151
|ACS Synthetic Biology 7/7||2020-01-27|
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