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Direct Pathway Cloning of Neglected Bacteria in the Hunt for Novel (Bio-)Chemistry

Total Cost €


EC-Contrib. €






 DiPaC_MC project word cloud

Explore the words cloud of the DiPaC_MC project. It provides you a very rough idea of what is the project "DiPaC_MC" about.

revolutionise    chemical    genetic    rare    identification    nchen    vectors    express    biology    utilise    assembly    chemistry    genome    global    source    completely    gene    overcome    resurgence    heterologously    threats    und       alteration    toolkit    auml    biomedical    health    characterisation    biochemistry    outcomes    sources    ziel    hrungs    uncharacterized    engineering    provides    methodology    constructed    fast    metabolic    cloning    amplicon    biosynthesis    discover    synthesis    resistance    ern    uuml    enzymes    gibson    expression    coupled    combination    downstream    zentralinstitut    antibiotic    biocatalytic    regards    mining    unusual    technische    heterologous    bacterial    pcr    poorly    unexplored    isolates    direct    synthetic    host    prolific    bioactive    discovery    organisms    immensely    vital    diversity    revealed    potentially    universit    clusters    harbor    incorporation    bio    natural    discovering    structural    techniques    bacteria    loci    20kb    microbial    lebensmittelforschung   

Project "DiPaC_MC" data sheet

The following table provides information about the project.


Organization address
address: Arcisstrasse 21
postcode: 80333

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Germany [DE]
 Total cost 159˙460 €
 EC max contribution 159˙460 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2016
 Funding Scheme MSCA-IF-EF-ST
 Starting year 2017
 Duration (year-month-day) from 2017-09-01   to  2019-08-31


Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 


 Project objective

To overcome recent global health threats, such as antibiotic resistance, a resurgence in the discovery of new chemical and thus biomedical diversity from microbial sources is needed. Genome mining in combination with heterologous expression is an approach that will overcome these challenges. It involves incorporation of yet uncharacterized natural product genetic loci into a fast growing heterologous host. For this approach, a prolific source of novel natural product gene clusters is vital. Our collaboration with the Zentralinstitut für Ernährungs- und Lebensmittelforschung (ZIEL, Technische Universität München) provides direct access to bacterial isolates completely unexplored in regards to their potential for natural product biosynthesis. Our genome analyses have revealed that many of the ZIEL isolates harbor a large number of uncharacterized natural product gene clusters. Thus, the aim of this proposal is to heterologously express natural product gene clusters from these neglected bacteria to discover novel natural product (bio-)chemistry. Because these organisms are poorly studied, the likelihood of discovering rare or novel biochemistry is immensely increased. Here, we will utilise a novel combination of synthetic biology techniques referred to as Direct Pathway Cloning. This will enable expression vectors to be constructed by large-amplicon PCR (up to 20kb) coupled to Gibson assembly. Development of the methodology is set to revolutionise synthetic biology and metabolic engineering. Downstream outcomes of this proposal will be the identification of novel, potentially bioactive natural products, the characterisation of unusual biochemistry and the addition of enzymes to the ‘biocatalytic toolkit’ for natural product synthesis and structural alteration.


year authors and title journal last update
List of publications.
2020 Marija Mojicevic, Paul M. D\'Agostino, Aleksandar Pavic, Sandra Vojnovic, Ramsankar Senthamaraikannan, Branka Vasiljevic, Tobias A. M. Gulder, Jasmina Nikodinovic‐Runic
Streptomyces sp. BV410 isolate from chamomile rhizosphere soil efficiently produces staurosporine with antifungal and antiangiogenic properties
published pages: , ISSN: 2045-8827, DOI: 10.1002/mbo3.986
MicrobiologyOpen 9/3 2020-04-15
2019 Elke R. Duell, Paul M. D’Agostino, Nicole Shapiro, Tanja Woyke, Thilo M. Fuchs, Tobias A. M. Gulder
Direct pathway cloning of the sodorifen biosynthetic gene cluster and recombinant generation of its product in E. coli
published pages: , ISSN: 1475-2859, DOI: 10.1186/s12934-019-1080-6
Microbial Cell Factories 18/1 2020-01-27
2018 Paul M. D’Agostino, Tobias A. M. Gulder
Direct Pathway Cloning Combined with Sequence- and Ligation-Independent Cloning for Fast Biosynthetic Gene Cluster Refactoring and Heterologous Expression
published pages: 1702-1708, ISSN: 2161-5063, DOI: 10.1021/acssynbio.8b00151
ACS Synthetic Biology 7/7 2020-01-27

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