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Direct Pathway Cloning of Neglected Bacteria in the Hunt for Novel (Bio-)Chemistry

Total Cost €


EC-Contrib. €






 DiPaC_MC project word cloud

Explore the words cloud of the DiPaC_MC project. It provides you a very rough idea of what is the project "DiPaC_MC" about.

20kb    clusters    sources    outcomes    biomedical    uncharacterized    hrungs    downstream    isolates    incorporation    utilise    biochemistry    chemistry    lebensmittelforschung    uuml    discover    universit    revealed    metabolic    revolutionise    bacteria    expression    auml    mining    completely    biology    enzymes    structural    alteration    unusual    poorly    techniques    direct    antibiotic    vectors    potentially    pcr    threats    gene    resistance       synthetic    engineering    bacterial    express    cloning    coupled    biosynthesis    vital    bioactive    discovering    global    organisms    assembly    identification    host    methodology    gibson    genome    technische    und    overcome    chemical    diversity    combination    heterologous    characterisation    unexplored    provides    genetic    rare    heterologously    fast    regards    loci    source    amplicon    zentralinstitut    bio    resurgence    harbor    toolkit    immensely    natural    microbial    health    prolific    discovery    synthesis    biocatalytic    ziel    constructed    nchen    ern   

Project "DiPaC_MC" data sheet

The following table provides information about the project.


Organization address
address: Arcisstrasse 21
postcode: 80333

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Germany [DE]
 Total cost 159˙460 €
 EC max contribution 159˙460 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2016
 Funding Scheme MSCA-IF-EF-ST
 Starting year 2017
 Duration (year-month-day) from 2017-09-01   to  2019-08-31


Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 


 Project objective

To overcome recent global health threats, such as antibiotic resistance, a resurgence in the discovery of new chemical and thus biomedical diversity from microbial sources is needed. Genome mining in combination with heterologous expression is an approach that will overcome these challenges. It involves incorporation of yet uncharacterized natural product genetic loci into a fast growing heterologous host. For this approach, a prolific source of novel natural product gene clusters is vital. Our collaboration with the Zentralinstitut für Ernährungs- und Lebensmittelforschung (ZIEL, Technische Universität München) provides direct access to bacterial isolates completely unexplored in regards to their potential for natural product biosynthesis. Our genome analyses have revealed that many of the ZIEL isolates harbor a large number of uncharacterized natural product gene clusters. Thus, the aim of this proposal is to heterologously express natural product gene clusters from these neglected bacteria to discover novel natural product (bio-)chemistry. Because these organisms are poorly studied, the likelihood of discovering rare or novel biochemistry is immensely increased. Here, we will utilise a novel combination of synthetic biology techniques referred to as Direct Pathway Cloning. This will enable expression vectors to be constructed by large-amplicon PCR (up to 20kb) coupled to Gibson assembly. Development of the methodology is set to revolutionise synthetic biology and metabolic engineering. Downstream outcomes of this proposal will be the identification of novel, potentially bioactive natural products, the characterisation of unusual biochemistry and the addition of enzymes to the ‘biocatalytic toolkit’ for natural product synthesis and structural alteration.


year authors and title journal last update
List of publications.
2020 Marija Mojicevic, Paul M. D\'Agostino, Aleksandar Pavic, Sandra Vojnovic, Ramsankar Senthamaraikannan, Branka Vasiljevic, Tobias A. M. Gulder, Jasmina Nikodinovic‐Runic
Streptomyces sp. BV410 isolate from chamomile rhizosphere soil efficiently produces staurosporine with antifungal and antiangiogenic properties
published pages: , ISSN: 2045-8827, DOI: 10.1002/mbo3.986
MicrobiologyOpen 9/3 2020-04-15
2019 Elke R. Duell, Paul M. D’Agostino, Nicole Shapiro, Tanja Woyke, Thilo M. Fuchs, Tobias A. M. Gulder
Direct pathway cloning of the sodorifen biosynthetic gene cluster and recombinant generation of its product in E. coli
published pages: , ISSN: 1475-2859, DOI: 10.1186/s12934-019-1080-6
Microbial Cell Factories 18/1 2020-01-27
2018 Paul M. D’Agostino, Tobias A. M. Gulder
Direct Pathway Cloning Combined with Sequence- and Ligation-Independent Cloning for Fast Biosynthetic Gene Cluster Refactoring and Heterologous Expression
published pages: 1702-1708, ISSN: 2161-5063, DOI: 10.1021/acssynbio.8b00151
ACS Synthetic Biology 7/7 2020-01-27

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