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LightZymes SIGNED

Evolution of artificial enzymes for light-driven reactions by implementing unnatural cofactors in protein scaffolds

Total Cost €

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EC-Contrib. €

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Partnership

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 LightZymes project word cloud

Explore the words cloud of the LightZymes project. It provides you a very rough idea of what is the project "LightZymes" about.

code    enzymes    supply    synthesis    evolution    stereoselectivities    catalysis    employing    enzymatic    photoorganocatalysis    proteins    create    conversions    photoredox    inside    facs    bio    cell    mass    expanding    enzyme    strength    radical    diversity    lightzymes    energy    canonical    engineering    cofactors    directed    cytoplasm    reactions    render    industrial    photo    envision    repertoire    bridging    modifications    combine    biocatalytic    amino    source    trna    organo    difficult    ncaa    organic    acids    first    huge    sustainable    instead    photosynthesis    substantially    form    biocatalysis    catalyzing    assembly    efficient    genetic    door    pcs    differs    ribosomal    pc    nature    chemists    selective    converting    economy    intermediates    drive    nadph    atp    time    expand    screening    variants    catalysts    redox    engineered    purifications    artificial    small    needing    synthetases    throughput    reactivities    light    asymmetric    catalyst    naturally    acyl    paves    photocatalysis    hybrid    perform    incorporate    generate    chemical    natural    spectrometry    preferentially    molecules    reaction    opens    regio   

Project "LightZymes" data sheet

The following table provides information about the project.

Coordinator
UNIVERSITAET GREIFSWALD 

Organization address
address: DOMSTRASSE 11
city: GREIFSWALD
postcode: 17489
website: www.uni-greifswald.de

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Germany [DE]
 Total cost 1˙498˙749 €
 EC max contribution 1˙498˙749 € (100%)
 Programme 1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC))
 Code Call ERC-2017-STG
 Funding Scheme ERC-STG
 Starting year 2018
 Duration (year-month-day) from 2018-02-01   to  2023-01-31

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    UNIVERSITAET GREIFSWALD DE (GREIFSWALD) coordinator 1˙498˙749.00

Map

 Project objective

The LightZymes project aims to create artificial enzymes (LightZymes) catalyzing selective light-driven conversions of small organic molecules. Enzyme catalysis has a large potential in the development of a sustainable, bio-based economy and is increasingly applied on industrial scale. Nature’s repertoire of enzymatic reactions is huge, but for many reactions developed by chemists, no natural enzyme is available. I envision expanding the chemical diversity of enzymes to photoredox catalysis. Chemists perform this type of reactions by employing photo(organo) redox catalysts (PC). However, achieving regio- and stereoselectivities is challenging, because radical intermediates generated during the reaction are difficult to control. To solve this problem, I will combine the strength of bio- and photocatalysis: organic PCs as artificial cofactors provide new reactivities, and the proteins will be evolved to render the reactions highly selective. This approach differs from artificial photosynthesis: instead converting light energy in high-energy cofactors (NADPH, ATP), light will directly enable selective synthesis reactions. Efficient directed evolution requires an easy assembly of the catalyst, preferentially inside the cell. I propose to apply genetic code engineering and to supply the PC in the form of non-canonical amino acids (ncAA). Engineered amino acyl tRNA synthetases will incorporate the PC directly during ribosomal synthesis. This will facilitate–for the first time–the assembly of hybrid catalysts in the cytoplasm without needing further modifications or purifications. This opens the door for applying high-throughput screening based on mass spectrometry and FACS to generate highly selective variants. By bridging the concepts of photoorganocatalysis and biocatalysis, LightZymes will substantially expand the chemical repertoire of naturally evolved enzymes. This paves the way to directly using light as energy source to drive biocatalytic asymmetric reactions.

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The information about "LIGHTZYMES" are provided by the European Opendata Portal: CORDIS opendata.

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