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LightZymes SIGNED

Evolution of artificial enzymes for light-driven reactions by implementing unnatural cofactors in protein scaffolds

Total Cost €

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EC-Contrib. €

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Partnership

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 LightZymes project word cloud

Explore the words cloud of the LightZymes project. It provides you a very rough idea of what is the project "LightZymes" about.

render    lightzymes    small    needing    energy    radical    proteins    directed    enzymes    intermediates    molecules    ribosomal    substantially    acids    reaction    enzyme    ncaa    naturally    cofactors    huge    catalyzing    nature    biocatalytic    selective    redox    drive    catalyst    bio    incorporate    stereoselectivities    canonical    perform    purifications    repertoire    genetic    create    cytoplasm    pc    modifications    supply    amino    diversity    chemical    synthesis    enzymatic    organo    mass    spectrometry    time    employing    converting    expand    engineered    conversions    reactivities    assembly    nadph    paves    sustainable    door    evolution    catalysis    photocatalysis    variants    natural    regio    reactions    opens    pcs    acyl    cell    source    photoorganocatalysis    code    differs    facs    first    atp    economy    throughput    biocatalysis    efficient    trna    instead    photo    catalysts    industrial    envision    expanding    asymmetric    preferentially    light    screening    difficult    combine    inside    form    generate    organic    hybrid    strength    photosynthesis    chemists    engineering    bridging    artificial    photoredox    synthetases   

Project "LightZymes" data sheet

The following table provides information about the project.

Coordinator
UNIVERSITAET GREIFSWALD 

Organization address
address: DOMSTRASSE 11
city: GREIFSWALD
postcode: 17489
website: www.uni-greifswald.de

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Germany [DE]
 Total cost 1˙498˙749 €
 EC max contribution 1˙498˙749 € (100%)
 Programme 1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC))
 Code Call ERC-2017-STG
 Funding Scheme ERC-STG
 Starting year 2018
 Duration (year-month-day) from 2018-02-01   to  2023-01-31

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    UNIVERSITAET GREIFSWALD DE (GREIFSWALD) coordinator 1˙498˙749.00

Map

 Project objective

The LightZymes project aims to create artificial enzymes (LightZymes) catalyzing selective light-driven conversions of small organic molecules. Enzyme catalysis has a large potential in the development of a sustainable, bio-based economy and is increasingly applied on industrial scale. Nature’s repertoire of enzymatic reactions is huge, but for many reactions developed by chemists, no natural enzyme is available. I envision expanding the chemical diversity of enzymes to photoredox catalysis. Chemists perform this type of reactions by employing photo(organo) redox catalysts (PC). However, achieving regio- and stereoselectivities is challenging, because radical intermediates generated during the reaction are difficult to control. To solve this problem, I will combine the strength of bio- and photocatalysis: organic PCs as artificial cofactors provide new reactivities, and the proteins will be evolved to render the reactions highly selective. This approach differs from artificial photosynthesis: instead converting light energy in high-energy cofactors (NADPH, ATP), light will directly enable selective synthesis reactions. Efficient directed evolution requires an easy assembly of the catalyst, preferentially inside the cell. I propose to apply genetic code engineering and to supply the PC in the form of non-canonical amino acids (ncAA). Engineered amino acyl tRNA synthetases will incorporate the PC directly during ribosomal synthesis. This will facilitate–for the first time–the assembly of hybrid catalysts in the cytoplasm without needing further modifications or purifications. This opens the door for applying high-throughput screening based on mass spectrometry and FACS to generate highly selective variants. By bridging the concepts of photoorganocatalysis and biocatalysis, LightZymes will substantially expand the chemical repertoire of naturally evolved enzymes. This paves the way to directly using light as energy source to drive biocatalytic asymmetric reactions.

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The information about "LIGHTZYMES" are provided by the European Opendata Portal: CORDIS opendata.

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