EU H2020 Project "PROTEASENTER (Disseminating N-terminomics to Advance Protease Research)": description, partecipants, costs and EC-fundings

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ProteaseNter project word cloud

Explore the words cloud of the ProteaseNter project. It provides you a very rough idea of what is the project "ProteaseNter" about.

initiation uncovering add laboratories relevance proteomic terminomic minimization thousands toolbox biological niche generation internal lack supply explore standards proteolysis differential kit local functional paraffin initial endeavor commercial manner modifications multiple material translation disposable assays annotation proteomics tumors native workflow bears foundation published expert solid inclusion fixed spectrometry formats branch rationale companies unbiased ready molecular phosphorylation wealth format sized small sites formalin samples proteome pathology bladder preparation protein comparability cryopreserved inaccessible laying yielding proteolytically backed termini terminomics online acetylated adaption proteogenomics laboratory fill remained serves cleavage cancer sculpts data limited services unlike pharmaceutical mass infiltration diagnostics input web collaborative sole

Project "ProteaseNter" data sheet

The following table provides information about the project.

Coordinator	UNIVERSITAETSKLINIKUM FREIBURG Organization address address: HUGSTETTER STRASSE 49 city: FREIBURG postcode: 79106 website: n.a. contact info title: n.a. name: n.a. surname: n.a. function: n.a. email: n.a. telephone: n.a. fax: n.a.
Coordinator Country	Germany [DE]
Total cost	149˙900 €
EC max contribution	149˙900 € (100%)
Programme	1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC))
Code Call	ERC-2017-PoC
Funding Scheme	ERC-POC
Starting year	2018
Duration (year-month-day)	from 2018-01-01 to 2019-06-30

#	participants	country	role	EC contrib. [€]
1	UNIVERSITAETSKLINIKUM FREIBURG	DE (FREIBURG)	coordinator	149˙900.00

UNIVERSITAETSKLINIKUM FREIBURG

DE (FREIBURG)

coordinator

149˙900.00

Project objective

Protein N-termini represent a wealth of biological information, such as their generation by limited proteolysis, which sculpts proteome functionality by yielding protein-sized cleavage products with novel functionality. N-terminomics is a branch of functional proteomics and enables the analysis of thousands of protein N-termini in an unbiased manner. By targeting native, proteolytically generated or acetylated protein N-termini as well as uncovering novel translation initiation sites, N-terminomics bears relevance to proteolysis and proteogenomics research. Unlike proteomic assays to study modifications such as phosphorylation, N-terminomics has remained inaccessible to non-expert laboratories due to a lack of ready-to-use kit formats or commercial services. Based on a recently published N-terminomic workflow by my laboratory, the present project aims to fill this niche by laying the foundation to represent N-terminomics (sample preparation up to mass spectrometry analysis) in a kit-like format together with online data analysis. Development steps include inclusion of multiple internal standards for improved sample-to-sample comparability, sole usage of cost-effective, disposable “small-scale” equipment in sample preparation, minimization of required input material, adaption to different input material including cryopreserved and formalin-fixed, paraffin-embedded samples, as well as web-based mass spectrometry data analysis and annotation. This endeavor is based on multiple collaborative projects of the applicant with pharmaceutical companies and backed by interest of proteomic supply companies. In a small forward-looking activity, the project will explore the application N-terminomics in molecular diagnostics of bladder cancer; based on the rationale local infiltration by solid tumors is based on differential proteolysis. This activity serves an initial step to add N-terminomics to the toolbox of molecular pathology.

Publications

List of publications.
year	authors and title	journal	last update
2018	Melanie Christine FÃ¶ll, Matthias Fahrner, Victor Oginga Oria, Markus KÃ¼hs, Martin Lothar Biniossek, Martin Werner, Peter Bronsert, Oliver Schilling Reproducible proteomics sample preparation for single FFPE tissue slices using acid-labile surfactant and direct trypsinization published pages: , ISSN: 1542-6416, DOI: 10.1186/s12014-018-9188-y	Clinical Proteomics 15/1	2020-01-21

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