ProteaseNter
Disseminating N-terminomics to Advance Protease Research
Total Cost €
0EC-Contrib. €
0Partnership
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0ProteaseNter project word cloud
Explore the words cloud of the ProteaseNter project. It provides you a very rough idea of what is the project "ProteaseNter" about.
Project "ProteaseNter" data sheet
The following table provides information about the project.
| Coordinator |
UNIVERSITAETSKLINIKUM FREIBURG
Organization address contact info |
| Coordinator Country | Germany [DE] |
| Total cost | 149˙900 € |
| EC max contribution | 149˙900 € (100%) |
| Programme |
1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC)) |
| Code Call | ERC-2017-PoC |
| Funding Scheme | ERC-POC |
| Starting year | 2018 |
| Duration (year-month-day) | from 2018-01-01 to 2019-06-30 |
Partnership
Take a look of project's partnership.
| # | ||||
|---|---|---|---|---|
| 1 | UNIVERSITAETSKLINIKUM FREIBURG | DE (FREIBURG) | coordinator | 149˙900.00 |
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Project objective
Protein N-termini represent a wealth of biological information, such as their generation by limited proteolysis, which sculpts proteome functionality by yielding protein-sized cleavage products with novel functionality. N-terminomics is a branch of functional proteomics and enables the analysis of thousands of protein N-termini in an unbiased manner. By targeting native, proteolytically generated or acetylated protein N-termini as well as uncovering novel translation initiation sites, N-terminomics bears relevance to proteolysis and proteogenomics research. Unlike proteomic assays to study modifications such as phosphorylation, N-terminomics has remained inaccessible to non-expert laboratories due to a lack of ready-to-use kit formats or commercial services. Based on a recently published N-terminomic workflow by my laboratory, the present project aims to fill this niche by laying the foundation to represent N-terminomics (sample preparation up to mass spectrometry analysis) in a kit-like format together with online data analysis. Development steps include inclusion of multiple internal standards for improved sample-to-sample comparability, sole usage of cost-effective, disposable “small-scale” equipment in sample preparation, minimization of required input material, adaption to different input material including cryopreserved and formalin-fixed, paraffin-embedded samples, as well as web-based mass spectrometry data analysis and annotation. This endeavor is based on multiple collaborative projects of the applicant with pharmaceutical companies and backed by interest of proteomic supply companies. In a small forward-looking activity, the project will explore the application N-terminomics in molecular diagnostics of bladder cancer; based on the rationale local infiltration by solid tumors is based on differential proteolysis. This activity serves an initial step to add N-terminomics to the toolbox of molecular pathology.
Publications
| year | authors and title | journal | last update |
|---|---|---|---|
| 2018 |
Melanie Christine Föll, Matthias Fahrner, Victor Oginga Oria, Markus Kühs, Martin Lothar Biniossek, Martin Werner, Peter Bronsert, Oliver Schilling Reproducible proteomics sample preparation for single FFPE tissue slices using acid-labile surfactant and direct trypsinization published pages: , ISSN: 1542-6416, DOI: 10.1186/s12014-018-9188-y |
Clinical Proteomics 15/1 | 2020-01-21 |
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