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CRISPR-GQ SIGNED

Identifying the Capabilities and Limitations of CRISPR in Targeting G-quadruplex Forming Sequences: From Target Recognition to Gene Expression Regulation

Total Cost €

0

EC-Contrib. €

0

Partnership

0

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 CRISPR-GQ project word cloud

Explore the words cloud of the CRISPR-GQ project. It provides you a very rough idea of what is the project "CRISPR-GQ" about.

wish    dementia    editing    guide    pursue    course    frontotemporal    dna    vicinity    techniques    promoters    canonical    regulatory    limitations    molecule    sclerosis    telomeric    forefront    secondary    sites    interspaced    world    region    translation    small    regulation    amyotrophic    disorders    als    stabilizing    clustered    oncogenes    regulate    expression    myc    effort    werner    genes    regions    geared    suggested    therapeutic    sequences    remove    ftd    perform    cells    neurological    demonstrated    frequency    rna    absence    promoter    strand    quadruplex    inhibit    certain    transcription    forming    bulk    enormous    single    gq    assays    prevent    proteins    inability    underway    pqs    complementary    bloom    untranslated    tyrosine    molecules    concentration    cas9    lateral    syndromes    constructs    cas    elucidate    genome    structures    critical    crispr    variants    structure    genomic    precision    form    hydroxylase    potentially    specificity    human    palindromic    gene   

Project "CRISPR-GQ" data sheet

The following table provides information about the project.

Coordinator
TECHNISCHE UNIVERSITEIT DELFT 

Organization address
address: STEVINWEG 1
city: DELFT
postcode: 2628 CN
website: www.tudelft.nl

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Netherlands [NL]
 Project website https://sites.google.com/site/balcilab/research/mariecuriefellowshipreport
 Total cost 88˙799 €
 EC max contribution 88˙799 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2017
 Funding Scheme MSCA-IF-EF-ST
 Starting year 2018
 Duration (year-month-day) from 2018-07-01   to  2019-06-30

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    TECHNISCHE UNIVERSITEIT DELFT NL (DELFT) coordinator 88˙799.00

Map

 Project objective

Clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR associated proteins (Cas) have recently been at the forefront of genomic research due to their enormous potential at editing the genome with great precision and specificity. A world-wide effort is currently underway to test Cas proteins and their variants for applications geared towards genome editing for human cells. How CRISPR-Cas systems perform in editing sequences that form non-canonical DNA or RNA secondary structures or are in the vicinity of such structures is the problem we wish to pursue during the course of proposed studies. One such structure is the G-quadruplex (GQ), which has been demonstrated to form throughout the human genome, with particular concentration in telomeric sites, promoters, and 3’ and 5’ untranslated regions of RNA. The higher frequency of potentially GQ forming sequences (PQS) at such regulatory sites has suggested a potential role for these structures in transcription or translation level gene expression regulation. GQ formation has been demonstrated to inhibit gene expression for a number of different genes, including certain critical oncogenes. Inability to remove these structures is directly associated with several syndromes, including Bloom and Werner syndromes, and neurological disorders such as amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD). Therefore, editing such sequences to prevent GQ formation also has therapeutic potential. We will design different single guide RNA constructs and target the GQ forming G-rich or the complementary C-rich strand to elucidate the capabilities and limitations of the CRISPR-Cas9 system in editing such structures using single molecule techniques and bulk assays. We will then investigate whether CRISPR-Cas9 can be used to regulate gene expression by targeting GQ structures in the promoter region of tyrosine hydroxylase and c-Myc genes in the presence and absence of GQ stabilizing small molecules.

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The information about "CRISPR-GQ" are provided by the European Opendata Portal: CORDIS opendata.

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