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CRISPR-GQ SIGNED

Identifying the Capabilities and Limitations of CRISPR in Targeting G-quadruplex Forming Sequences: From Target Recognition to Gene Expression Regulation

Total Cost €

0

EC-Contrib. €

0

Partnership

0

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 CRISPR-GQ project word cloud

Explore the words cloud of the CRISPR-GQ project. It provides you a very rough idea of what is the project "CRISPR-GQ" about.

forefront    region    inhibit    bloom    regions    proteins    suggested    editing    werner    gq    underway    pursue    single    concentration    quadruplex    promoters    promoter    hydroxylase    small    sites    therapeutic    demonstrated    bulk    regulation    variants    untranslated    form    precision    regulate    prevent    dna    gene    frontotemporal    lateral    ftd    als    genome    clustered    human    geared    transcription    amyotrophic    absence    assays    wish    telomeric    disorders    molecules    sclerosis    pqs    techniques    specificity    cas9    syndromes    elucidate    perform    world    stabilizing    vicinity    frequency    rna    constructs    remove    oncogenes    potentially    dementia    crispr    genes    neurological    guide    structure    forming    enormous    secondary    cas    regulatory    cells    molecule    course    complementary    effort    translation    critical    certain    tyrosine    myc    strand    palindromic    expression    limitations    sequences    interspaced    inability    genomic    structures    canonical   

Project "CRISPR-GQ" data sheet

The following table provides information about the project.

Coordinator
TECHNISCHE UNIVERSITEIT DELFT 

Organization address
address: STEVINWEG 1
city: DELFT
postcode: 2628 CN
website: www.tudelft.nl

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Netherlands [NL]
 Project website https://sites.google.com/site/balcilab/research/mariecuriefellowshipreport
 Total cost 88˙799 €
 EC max contribution 88˙799 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2017
 Funding Scheme MSCA-IF-EF-ST
 Starting year 2018
 Duration (year-month-day) from 2018-07-01   to  2019-06-30

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    TECHNISCHE UNIVERSITEIT DELFT NL (DELFT) coordinator 88˙799.00

Map

 Project objective

Clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR associated proteins (Cas) have recently been at the forefront of genomic research due to their enormous potential at editing the genome with great precision and specificity. A world-wide effort is currently underway to test Cas proteins and their variants for applications geared towards genome editing for human cells. How CRISPR-Cas systems perform in editing sequences that form non-canonical DNA or RNA secondary structures or are in the vicinity of such structures is the problem we wish to pursue during the course of proposed studies. One such structure is the G-quadruplex (GQ), which has been demonstrated to form throughout the human genome, with particular concentration in telomeric sites, promoters, and 3’ and 5’ untranslated regions of RNA. The higher frequency of potentially GQ forming sequences (PQS) at such regulatory sites has suggested a potential role for these structures in transcription or translation level gene expression regulation. GQ formation has been demonstrated to inhibit gene expression for a number of different genes, including certain critical oncogenes. Inability to remove these structures is directly associated with several syndromes, including Bloom and Werner syndromes, and neurological disorders such as amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD). Therefore, editing such sequences to prevent GQ formation also has therapeutic potential. We will design different single guide RNA constructs and target the GQ forming G-rich or the complementary C-rich strand to elucidate the capabilities and limitations of the CRISPR-Cas9 system in editing such structures using single molecule techniques and bulk assays. We will then investigate whether CRISPR-Cas9 can be used to regulate gene expression by targeting GQ structures in the promoter region of tyrosine hydroxylase and c-Myc genes in the presence and absence of GQ stabilizing small molecules.

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The information about "CRISPR-GQ" are provided by the European Opendata Portal: CORDIS opendata.

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