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Amitochondriates SIGNED

Life without mitochondrion

Total Cost €

0

EC-Contrib. €

0

Partnership

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 Amitochondriates project word cloud

Explore the words cloud of the Amitochondriates project. It provides you a very rough idea of what is the project "Amitochondriates" about.

axenic    time    moments    eukaryotes    induce    functions    knock    eukaryotic    cell    thought    create    houses    eukaryote    organism    cluster    genomics    invited    biogenesis    thriving    conduct    dissection    laboratory    experts    potentially    metabolic    form    elucidating    details    giardia    assembly    additional    team    motivations    2016    genes    amitochondriate    representing    functional    lineages    transcriptomics    models    flagellate    minimalistic    monocercomonoides    concentrate    perform    culture    mitochondrial    eight    cells    transforming    power    pi    discovered    simulating    sulphur    experiments    circumstances    vivo    tools    metabolomic    iron    vitro    histolytica    revealing    chosen    successful    possibilities    hope    examine    physiology    compartments    latter    questions    event    knocking    intestinalis    genetic    cultivation    mitosomes    until    model    biological    manipulation    mitochondria    first    entamoeba    cellular    tractable    outs   

Project "Amitochondriates" data sheet

The following table provides information about the project.

Coordinator
UNIVERZITA KARLOVA 

Organization address
address: OVOCNY TRH 560/5
city: PRAHA 1
postcode: 116 36
website: www.cuni.cz

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Czech Republic [CZ]
 Project website http://www.protistologie.cz/hampllab/
 Total cost 1˙935˙500 €
 EC max contribution 1˙935˙500 € (100%)
 Programme 1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC))
 Code Call ERC-2017-COG
 Funding Scheme ERC-COG
 Starting year 2018
 Duration (year-month-day) from 2018-05-01   to  2023-04-30

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    UNIVERZITA KARLOVA CZ (PRAHA 1) coordinator 1˙935˙500.00

Map

 Project objective

Mitochondria are often referred to as the “power houses” of eukaryotic cells. All eukaryotes were thought to have mitochondria of some form until 2016, when the first eukaryote thriving without mitochondria was discovered by our laboratory – a flagellate Monocercomonoides. Understanding cellular functions of these cells, which represent a new functional type of eukaryotes, and understanding the circumstances of the unique event of mitochondrial loss are motivations for this proposal. The first objective focuses on the cell physiology. We will perform a metabolomic study revealing major metabolic pathways and concentrate further on elucidating its unique system of iron-sulphur cluster assembly. In the second objective, we will investigate in details the unique case of mitochondrial loss. We will examine two additional potentially amitochondriate lineages by means of genomics and transcriptomics, conduct experiments simulating the moments of mitochondrial loss and try to induce the mitochondrial loss in vitro by knocking out or down genes for mitochondrial biogenesis. We have chosen Giardia intestinalis and Entamoeba histolytica as models for the latter experiments, because their mitochondria are already reduced to minimalistic “mitosomes” and because some genetic tools are already available for them. Successful mitochondrial knock-outs would enable us to study mitochondrial loss in ‘real time’ and in vivo. In the third objective, we will focus on transforming Monocercomonoides into a tractable laboratory model by developing methods of axenic cultivation and genetic manipulation. This will open new possibilities in the studies of this organism and create a cell culture representing an amitochondriate model for cell biological studies enabling the dissection of mitochondrial effects from those of other compartments. The team is composed of the laboratory of PI and eight invited experts and we hope it has the ability to address these challenging questions.

 Publications

year authors and title journal last update
List of publications.
2019 Sebastian C. Treitli, Martin Kolisko, Filip Husník, Patrick J. Keeling, Vladimír Hampl
Revealing the metabolic capacity of Streblomastix strix and its bacterial symbionts using single-cell metagenomics
published pages: 201910793, ISSN: 0027-8424, DOI: 10.1073/pnas.1910793116
Proceedings of the National Academy of Sciences 2019-09-17
2019 Anna Karnkowska, Sebastian C Treitli, Ondřej Brzoň, Lukáš Novák, Vojtěch Vacek, Petr Soukal, Lael D Barlow, Emily K Herman, Shweta V Pipaliya, Tomáš Pánek, David Žihala, Romana Petrželková, Anzhelika Butenko, Laura Eme, Courtney W Stairs, Andrew J Roger, Marek Eliáš, Joel B Dacks, Vladimír Hampl
The Oxymonad Genome Displays Canonical Eukaryotic Complexity in the Absence of a Mitochondrion
published pages: , ISSN: 0737-4038, DOI: 10.1093/molbev/msz147
Molecular Biology and Evolution 2019-09-17
2018 Vojtěch Vacek, Lukáš V F Novák, Sebastian C Treitli, Petr Táborský, Ivan Čepička, Martin Kolísko, Patrick J Keeling, Vladimír Hampl
Fe–S Cluster Assembly in Oxymonads and Related Protists
published pages: , ISSN: 0737-4038, DOI: 10.1093/molbev/msy168
Molecular Biology and Evolution 2019-05-08

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