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Amitochondriates SIGNED

Life without mitochondrion

Total Cost €

0

EC-Contrib. €

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Partnership

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 Amitochondriates project word cloud

Explore the words cloud of the Amitochondriates project. It provides you a very rough idea of what is the project "Amitochondriates" about.

mitochondrial    culture    houses    tractable    concentrate    flagellate    eight    moments    questions    eukaryotic    histolytica    outs    induce    circumstances    amitochondriate    cellular    axenic    pi    model    compartments    sulphur    first    functional    vitro    cell    knocking    genes    cells    entamoeba    perform    successful    simulating    potentially    representing    experiments    vivo    power    motivations    event    metabolomic    mitochondria    minimalistic    hope    models    manipulation    lineages    biological    chosen    cultivation    details    until    revealing    form    cluster    genomics    assembly    additional    giardia    invited    thriving    physiology    functions    dissection    eukaryote    metabolic    possibilities    monocercomonoides    conduct    knock    organism    discovered    team    experts    transforming    create    thought    time    latter    tools    elucidating    2016    laboratory    intestinalis    biogenesis    eukaryotes    transcriptomics    iron    examine    genetic    mitosomes   

Project "Amitochondriates" data sheet

The following table provides information about the project.

Coordinator
UNIVERZITA KARLOVA 

Organization address
address: OVOCNY TRH 560/5
city: PRAHA 1
postcode: 116 36
website: www.cuni.cz

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Czech Republic [CZ]
 Project website http://www.protistologie.cz/hampllab/
 Total cost 1˙935˙500 €
 EC max contribution 1˙935˙500 € (100%)
 Programme 1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC))
 Code Call ERC-2017-COG
 Funding Scheme ERC-COG
 Starting year 2018
 Duration (year-month-day) from 2018-05-01   to  2023-04-30

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    UNIVERZITA KARLOVA CZ (PRAHA 1) coordinator 1˙935˙500.00

Map

 Project objective

Mitochondria are often referred to as the “power houses” of eukaryotic cells. All eukaryotes were thought to have mitochondria of some form until 2016, when the first eukaryote thriving without mitochondria was discovered by our laboratory – a flagellate Monocercomonoides. Understanding cellular functions of these cells, which represent a new functional type of eukaryotes, and understanding the circumstances of the unique event of mitochondrial loss are motivations for this proposal. The first objective focuses on the cell physiology. We will perform a metabolomic study revealing major metabolic pathways and concentrate further on elucidating its unique system of iron-sulphur cluster assembly. In the second objective, we will investigate in details the unique case of mitochondrial loss. We will examine two additional potentially amitochondriate lineages by means of genomics and transcriptomics, conduct experiments simulating the moments of mitochondrial loss and try to induce the mitochondrial loss in vitro by knocking out or down genes for mitochondrial biogenesis. We have chosen Giardia intestinalis and Entamoeba histolytica as models for the latter experiments, because their mitochondria are already reduced to minimalistic “mitosomes” and because some genetic tools are already available for them. Successful mitochondrial knock-outs would enable us to study mitochondrial loss in ‘real time’ and in vivo. In the third objective, we will focus on transforming Monocercomonoides into a tractable laboratory model by developing methods of axenic cultivation and genetic manipulation. This will open new possibilities in the studies of this organism and create a cell culture representing an amitochondriate model for cell biological studies enabling the dissection of mitochondrial effects from those of other compartments. The team is composed of the laboratory of PI and eight invited experts and we hope it has the ability to address these challenging questions.

 Publications

year authors and title journal last update
List of publications.
2019 Sebastian C. Treitli, Martin Kolisko, Filip Husník, Patrick J. Keeling, Vladimír Hampl
Revealing the metabolic capacity of Streblomastix strix and its bacterial symbionts using single-cell metagenomics
published pages: 201910793, ISSN: 0027-8424, DOI: 10.1073/pnas.1910793116
Proceedings of the National Academy of Sciences 2019-09-17
2019 Anna Karnkowska, Sebastian C Treitli, Ondřej Brzoň, Lukáš Novák, Vojtěch Vacek, Petr Soukal, Lael D Barlow, Emily K Herman, Shweta V Pipaliya, Tomáš Pánek, David Žihala, Romana Petrželková, Anzhelika Butenko, Laura Eme, Courtney W Stairs, Andrew J Roger, Marek Eliáš, Joel B Dacks, Vladimír Hampl
The Oxymonad Genome Displays Canonical Eukaryotic Complexity in the Absence of a Mitochondrion
published pages: , ISSN: 0737-4038, DOI: 10.1093/molbev/msz147
Molecular Biology and Evolution 2019-09-17
2018 Vojtěch Vacek, Lukáš V F Novák, Sebastian C Treitli, Petr Táborský, Ivan Čepička, Martin Kolísko, Patrick J Keeling, Vladimír Hampl
Fe–S Cluster Assembly in Oxymonads and Related Protists
published pages: , ISSN: 0737-4038, DOI: 10.1093/molbev/msy168
Molecular Biology and Evolution 2019-05-08

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