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MS SPIDOC SIGNED

Mass Spectrometry for Single Particle Imaging of Dipole Oriented protein Complexes

Total Cost €

EC-Contrib. €

Partnership

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Outcomes and
results

MS SPIDOC project word cloud

Explore the words cloud of the MS SPIDOC project. It provides you a very rough idea of what is the project "MS SPIDOC" about.

protein strengthen times operation conformational particles smaller biomolecular consumption time xfel limited spidoc brilliance identification structure data computer area wealth reconstruction instrument electron separated particle opened speeding facility stage ray era bottleneck gas biomolecules combined environment rate repetition overcome movies peak em free structures spectrometry components internationally thereby easing microscopy transient accessible tremendously named unfolding resolution derive viral generation background orientation pattern oriented simulations combines dipole lasers ms yielding complexes single mass proteins renders risk molecular native market reconstruct spi expertise datasets machines orient axis industry entered image conformation imaging unparalleled structural give crystallization

Project "MS SPIDOC" data sheet

The following table provides information about the project.

Coordinator	HEINRICH-PETTE INSTITUT LEIBNIZ INSTITUT FUER EXPERIMENTELLE VIROLOGIE Organization address address: MARTINISTRASSE 52 city: HAMBURG postcode: 20251 website: www.hpi-hamburg.de contact info title: n.a. name: n.a. surname: n.a. function: n.a. email: n.a. telephone: n.a. fax: n.a.
Coordinator Country	Germany [DE]
Project website	http://www.ms-spidoc.eu
Total cost	3˙730˙000 €
EC max contribution	3˙730˙000 € (100%)
Programme	1. H2020-EU.1.2.1. (FET Open)
Code Call	H2020-FETOPEN-1-2016-2017
Funding Scheme	RIA
Starting year	2018
Duration (year-month-day)	from 2018-09-01 to 2021-08-31

Partnership

Take a look of project's partnership.

#	participants	country	role	EC contrib. [€]
1	HEINRICH-PETTE INSTITUT LEIBNIZ INSTITUT FUER EXPERIMENTELLE VIROLOGIE HEINRICH-PETTE INSTITUT LEIBNIZ INSTITUT FUER EXPERIMENTELLE VIROLOGIE Organization address address: MARTINISTRASSE 52 city: HAMBURG postcode: 20251 website: www.hpi-hamburg.de contact info title: n.a. name: n.a. surname: n.a. function: n.a. email: n.a. telephone: n.a. fax: n.a.	DE (HAMBURG)	coordinator	499˙125.00
2	EUROPEAN X-RAY FREE-ELECTRON LASERFACILITY GMBH EUROPEAN X-RAY FREE-ELECTRON LASERFACILITY GMBH Organization address address: HOLZKOPPEL 4 city: SCHENEFELD postcode: 22869 website: www.xfel.eu contact info title: n.a. name: n.a. surname: n.a. function: n.a. email: n.a. telephone: n.a. fax: n.a.	DE (SCHENEFELD)	participant	600˙000.00
3	FASMATECH EPISTIMONIKI KAI TECHNOLOGIKI ANONYMI ETAIREIA FASMATECH EPISTIMONIKI KAI TECHNOLOGIKI ANONYMI ETAIREIA Organization address address: 28TH KLM ATHINA LAMIA NATIONAL ROAD city: AFIDNES ATTIKIS postcode: 19014 website: n.a. contact info title: n.a. name: n.a. surname: n.a. function: n.a. email: n.a. telephone: n.a. fax: n.a.	EL (AFIDNES ATTIKIS)	participant	587˙500.00
4	UPPSALA UNIVERSITET UPPSALA UNIVERSITET Organization address address: VON KRAEMERS ALLE 4 city: UPPSALA postcode: 751 05 website: www.uu.se contact info title: n.a. name: n.a. surname: n.a. function: n.a. email: n.a. telephone: n.a. fax: n.a.	SE (UPPSALA)	participant	512˙500.00
5	SPECTROMETRY VISION BV SPECTROMETRY VISION BV Organization address address: TELEVISIEWEG 40 city: ALMERE postcode: 1322 AM website: n.a. contact info title: n.a. name: n.a. surname: n.a. function: n.a. email: n.a. telephone: n.a. fax: n.a.	NL (ALMERE)	participant	432˙500.00
6	UNIVERSITAET GREIFSWALD UNIVERSITAET GREIFSWALD Organization address address: DOMSTRASSE 11 city: GREIFSWALD postcode: 17489 website: www.uni-greifswald.de contact info title: n.a. name: n.a. surname: n.a. function: n.a. email: n.a. telephone: n.a. fax: n.a.	DE (GREIFSWALD)	participant	421˙000.00
7	THE UNIVERSITY OF MANCHESTER THE UNIVERSITY OF MANCHESTER Organization address address: OXFORD ROAD city: MANCHESTER postcode: M13 9PL website: www.manchester.ac.uk contact info title: n.a. name: n.a. surname: n.a. function: n.a. email: n.a. telephone: n.a. fax: n.a.	UK (MANCHESTER)	participant	362˙375.00
8	UNIVERSITE LYON 1 CLAUDE BERNARD UNIVERSITE LYON 1 CLAUDE BERNARD Organization address address: BOULEVARD DU 11 NOVEMBRE 1918 NUM43 city: VILLEURBANNE CEDEX postcode: 69622 website: www.univ-Iyon1.fr contact info title: n.a. name: n.a. surname: n.a. function: n.a. email: n.a. telephone: n.a. fax: n.a.	FR (VILLEURBANNE CEDEX)	participant	315˙000.00

Map

Project objective

The European XFEL has just entered user operation. With its unparalleled peak brilliance and repetition rate, European XFEL has the potential to further applications in single particle imaging (SPI), thus far limited to large viral particles at X-ray Free-Electron Lasers (XFEL). SPI will allow imaging protein complexes without the need for crystallization. This eventually renders transient conformational states accessible for high resolution structural studies yielding molecular movies of biomolecular machines. A major bottleneck is the wealth of data required to reconstruct a single structure leading to long processing times. This is currently also a problem in electron microscopy (EM). MS SPIDOC will overcome this data challenge by developing a native mass spectrometry (MS) system for sample delivery, named X-MS-I. It will provide mass and conformation selected biomolecules, which are oriented along their dipole axis upon imaging. This will enable structural reconstruction from much smaller datasets speeding up the analysis time tremendously. Moreover, the system features low sample consumption and a controlled low background easing pattern identification. The main objectives of the project are: • Deliver mass and conformation separated biomolecules for SPI. • Orient proteins for SPI. • Image protein complex unfolding • Exploit potential of protein orientation for other applications The MS SPIDOC consortium combines internationally leading expertise in different fields relevant to the project: Instrument design and development, computer simulations as well as working with biomolecules in the gas phase and on SPI are combined to implement the novel sample environment at the next generation XFEL facility. New components and methods will be opened to the market and thereby strengthen the European Research Area (ERA) and industry. This early stage high-risk project will give rise to a new technology with major impact on how to derive structures of biomolecules.

Deliverables

List of deliverables.
Dissemination and Exploitation plan	Documents, reports	2020-02-12 17:33:30
Instrumental settings for structural integrity	Documents, reports	2020-02-12 17:33:23
Data management plan	Open Research Data Pilot	2020-02-12 17:33:27
Stability range and charge distribution of electrosprayed ions	Documents, reports	2020-02-12 17:33:23
Public and internal web services, logo	Websites, patent fillings, videos etc.	2020-01-28 17:14:12

Take a look to the deliverables list in detail: detailed list of MS SPIDOC deliverables.

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