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MS SPIDOC SIGNED

Mass Spectrometry for Single Particle Imaging of Dipole Oriented protein Complexes

Total Cost €

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EC-Contrib. €

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Partnership

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 MS SPIDOC project word cloud

Explore the words cloud of the MS SPIDOC project. It provides you a very rough idea of what is the project "MS SPIDOC" about.

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Project "MS SPIDOC" data sheet

The following table provides information about the project.

Coordinator
HEINRICH-PETTE INSTITUT LEIBNIZ INSTITUT FUER EXPERIMENTELLE VIROLOGIE 

Organization address
address: MARTINISTRASSE 52
city: HAMBURG
postcode: 20251
website: www.hpi-hamburg.de

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
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fax: n.a.

 Coordinator Country Germany [DE]
 Project website http://www.ms-spidoc.eu
 Total cost 3˙730˙000 €
 EC max contribution 3˙730˙000 € (100%)
 Programme 1. H2020-EU.1.2.1. (FET Open)
 Code Call H2020-FETOPEN-1-2016-2017
 Funding Scheme RIA
 Starting year 2018
 Duration (year-month-day) from 2018-09-01   to  2021-08-31

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    HEINRICH-PETTE INSTITUT LEIBNIZ INSTITUT FUER EXPERIMENTELLE VIROLOGIE DE (HAMBURG) coordinator 499˙125.00
2    EUROPEAN X-RAY FREE-ELECTRON LASERFACILITY GMBH DE (SCHENEFELD) participant 600˙000.00
3    FASMATECH EPISTIMONIKI KAI TECHNOLOGIKI ANONYMI ETAIREIA EL (AFIDNES ATTIKIS) participant 587˙500.00
4    UPPSALA UNIVERSITET SE (UPPSALA) participant 512˙500.00
5    SPECTROMETRY VISION BV NL (ALMERE) participant 432˙500.00
6    UNIVERSITAET GREIFSWALD DE (GREIFSWALD) participant 421˙000.00
7    THE UNIVERSITY OF MANCHESTER UK (MANCHESTER) participant 362˙375.00
8    UNIVERSITE LYON 1 CLAUDE BERNARD FR (VILLEURBANNE CEDEX) participant 315˙000.00

Map

 Project objective

The European XFEL has just entered user operation. With its unparalleled peak brilliance and repetition rate, European XFEL has the potential to further applications in single particle imaging (SPI), thus far limited to large viral particles at X-ray Free-Electron Lasers (XFEL). SPI will allow imaging protein complexes without the need for crystallization. This eventually renders transient conformational states accessible for high resolution structural studies yielding molecular movies of biomolecular machines. A major bottleneck is the wealth of data required to reconstruct a single structure leading to long processing times. This is currently also a problem in electron microscopy (EM). MS SPIDOC will overcome this data challenge by developing a native mass spectrometry (MS) system for sample delivery, named X-MS-I. It will provide mass and conformation selected biomolecules, which are oriented along their dipole axis upon imaging. This will enable structural reconstruction from much smaller datasets speeding up the analysis time tremendously. Moreover, the system features low sample consumption and a controlled low background easing pattern identification. The main objectives of the project are: • Deliver mass and conformation separated biomolecules for SPI. • Orient proteins for SPI. • Image protein complex unfolding • Exploit potential of protein orientation for other applications The MS SPIDOC consortium combines internationally leading expertise in different fields relevant to the project: Instrument design and development, computer simulations as well as working with biomolecules in the gas phase and on SPI are combined to implement the novel sample environment at the next generation XFEL facility. New components and methods will be opened to the market and thereby strengthen the European Research Area (ERA) and industry. This early stage high-risk project will give rise to a new technology with major impact on how to derive structures of biomolecules.

 Deliverables

List of deliverables.
Dissemination and Exploitation plan Documents, reports 2020-02-12 17:33:30
Instrumental settings for structural integrity Documents, reports 2020-02-12 17:33:23
Data management plan Open Research Data Pilot 2020-02-12 17:33:27
Stability range and charge distribution of electrosprayed ions Documents, reports 2020-02-12 17:33:23
Public and internal web services, logo Websites, patent fillings, videos etc. 2020-01-28 17:14:12

Take a look to the deliverables list in detail:  detailed list of MS SPIDOC deliverables.

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The information about "MS SPIDOC" are provided by the European Opendata Portal: CORDIS opendata.

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