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HDPROBES SIGNED

Photoactivatable Sensors and Blinking Dyes for Live-Cell, Single-Molecule Localization Microscopy

Total Cost €

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EC-Contrib. €

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Partnership

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 HDPROBES project word cloud

Explore the words cloud of the HDPROBES project. It provides you a very rough idea of what is the project "HDPROBES" about.

single    signaling    resolution    light    observe    precluded    oxygen    localization    enzymes    switch    small    detect    reticulum    confinement    live    initiate    pathological    reactive    biological    emission    endoplasmic    dark    photoactivation    elusive    protein    densely    multicolor    unprecedented    equilibrium    takes    protease    dyes    agents    nanometric    active    migrates    hypothesized    cellular    forms    compartmentalization    mechanism    initial    molecules    toxic    methods    switching    regulate    emissive    microdomains    nucleus    wavelengths    observation    first    lapse    families    class    dynamic    blink    nitrogen    sensors    hour    cell    detecting    super    lack    endogenous    introduce    transcription    sites    perform    translocation    intractable    biochemical    lived    physiological    fluorescent    prepare    apoptotic    misfolding    specimens    details    species    molecule    spatiotemporal    intracellular    stress    time    probes    tools    palette    encompasses    intact    microscopy    imaging    tracking    investigation    subcellular    mechanisms    labeled   

Project "HDPROBES" data sheet

The following table provides information about the project.

Coordinator
ECOLE POLYTECHNIQUE FEDERALE DE LAUSANNE 

Organization address
address: BATIMENT CE 3316 STATION 1
city: LAUSANNE
postcode: 1015
website: www.epfl.ch

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Switzerland [CH]
 Total cost 1˙498˙125 €
 EC max contribution 1˙498˙125 € (100%)
 Programme 1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC))
 Code Call ERC-2018-STG
 Funding Scheme ERC-STG
 Starting year 2019
 Duration (year-month-day) from 2019-10-01   to  2024-09-30

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    ECOLE POLYTECHNIQUE FEDERALE DE LAUSANNE CH (LAUSANNE) coordinator 1˙498˙125.00

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 Project objective

In this proposal, we introduce two new families of probes for live-cell super-resolution microscopy. The first class comprises small-molecule fluorescent sensors for detecting short-lived, small signaling molecules and active enzymes with single-molecule resolution. The spatiotemporal confinement of biological reactive molecules has been hypothesized to regulate various pathological and physiological processes, but the lack of tools to observe directly these microdomains of biochemical activity has precluded the investigation of these mechanisms. The ability to detect small signaling agents and active enzymes with nanometric resolution in intact live specimens will allow us to study the role of compartmentalization in intracellular signaling at an unprecedented resolution. Our studies will focus on detecting elusive reactive oxygen and nitrogen species directly at their sites of endogenous production. We will also investigate the subcellular distribution of protease activity, focusing on its role in non-apoptotic signaling. The second class of probes encompasses a palette of fluorescent dyes that switch continuously between dark and emissive forms. This dynamic equilibrium will enable the localization of single molecules in a densely labeled field without the need to apply toxic light for photoactivation. Based on a novel switching mechanism, we will prepare dyes of various emission wavelengths that blink in a controlled way. These dyes will allow us to perform, for the first time, super-resolution, multicolor, time-lapse imaging of live specimens over long time. Initial studies will focus on tracking a transcription factor that migrates from the endoplasmic reticulum to the nucleus to initiate a cellular stress response upon protein misfolding. These studies will provide spatiotemporal details of this important translocation, which takes more than one hour to occur and its observation at the single-molecule level is intractable with current super-resolution methods

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The information about "HDPROBES" are provided by the European Opendata Portal: CORDIS opendata.

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