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HDPROBES SIGNED

Photoactivatable Sensors and Blinking Dyes for Live-Cell, Single-Molecule Localization Microscopy

Total Cost €

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EC-Contrib. €

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Partnership

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 HDPROBES project word cloud

Explore the words cloud of the HDPROBES project. It provides you a very rough idea of what is the project "HDPROBES" about.

tools    localization    detecting    stress    migrates    single    palette    intracellular    confinement    emission    mechanism    oxygen    encompasses    emissive    initial    multicolor    specimens    class    cell    live    transcription    translocation    dynamic    sites    reactive    nucleus    light    biochemical    perform    apoptotic    protease    misfolding    forms    intractable    lack    imaging    mechanisms    small    methods    unprecedented    probes    prepare    nanometric    biological    microscopy    dyes    investigation    protein    intact    signaling    densely    dark    subcellular    agents    elusive    lapse    endoplasmic    physiological    observation    sensors    molecule    observe    hypothesized    active    details    labeled    molecules    tracking    switch    photoactivation    families    toxic    compartmentalization    cellular    hour    species    endogenous    initiate    super    spatiotemporal    precluded    detect    reticulum    pathological    time    takes    nitrogen    fluorescent    lived    switching    first    wavelengths    enzymes    resolution    blink    microdomains    equilibrium    introduce    regulate   

Project "HDPROBES" data sheet

The following table provides information about the project.

Coordinator
ECOLE POLYTECHNIQUE FEDERALE DE LAUSANNE 

Organization address
address: BATIMENT CE 3316 STATION 1
city: LAUSANNE
postcode: 1015
website: www.epfl.ch

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Switzerland [CH]
 Total cost 1˙498˙125 €
 EC max contribution 1˙498˙125 € (100%)
 Programme 1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC))
 Code Call ERC-2018-STG
 Funding Scheme ERC-STG
 Starting year 2019
 Duration (year-month-day) from 2019-10-01   to  2024-09-30

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    ECOLE POLYTECHNIQUE FEDERALE DE LAUSANNE CH (LAUSANNE) coordinator 1˙498˙125.00

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 Project objective

In this proposal, we introduce two new families of probes for live-cell super-resolution microscopy. The first class comprises small-molecule fluorescent sensors for detecting short-lived, small signaling molecules and active enzymes with single-molecule resolution. The spatiotemporal confinement of biological reactive molecules has been hypothesized to regulate various pathological and physiological processes, but the lack of tools to observe directly these microdomains of biochemical activity has precluded the investigation of these mechanisms. The ability to detect small signaling agents and active enzymes with nanometric resolution in intact live specimens will allow us to study the role of compartmentalization in intracellular signaling at an unprecedented resolution. Our studies will focus on detecting elusive reactive oxygen and nitrogen species directly at their sites of endogenous production. We will also investigate the subcellular distribution of protease activity, focusing on its role in non-apoptotic signaling. The second class of probes encompasses a palette of fluorescent dyes that switch continuously between dark and emissive forms. This dynamic equilibrium will enable the localization of single molecules in a densely labeled field without the need to apply toxic light for photoactivation. Based on a novel switching mechanism, we will prepare dyes of various emission wavelengths that blink in a controlled way. These dyes will allow us to perform, for the first time, super-resolution, multicolor, time-lapse imaging of live specimens over long time. Initial studies will focus on tracking a transcription factor that migrates from the endoplasmic reticulum to the nucleus to initiate a cellular stress response upon protein misfolding. These studies will provide spatiotemporal details of this important translocation, which takes more than one hour to occur and its observation at the single-molecule level is intractable with current super-resolution methods

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The information about "HDPROBES" are provided by the European Opendata Portal: CORDIS opendata.

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