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HDPROBES SIGNED

Photoactivatable Sensors and Blinking Dyes for Live-Cell, Single-Molecule Localization Microscopy

Total Cost €

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EC-Contrib. €

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Partnership

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 HDPROBES project word cloud

Explore the words cloud of the HDPROBES project. It provides you a very rough idea of what is the project "HDPROBES" about.

detect    forms    perform    intracellular    fluorescent    intact    prepare    active    dynamic    apoptotic    densely    enzymes    microscopy    blink    detecting    mechanism    observation    lack    localization    observe    introduce    biological    labeled    live    misfolding    emission    time    dyes    imaging    unprecedented    initial    light    lapse    initiate    class    molecule    signaling    protein    lived    confinement    nucleus    first    species    cell    microdomains    palette    nanometric    precluded    molecules    photoactivation    details    wavelengths    mechanisms    dark    elusive    sites    agents    investigation    intractable    spatiotemporal    families    pathological    translocation    endogenous    equilibrium    reticulum    tools    small    compartmentalization    probes    emissive    oxygen    hypothesized    reactive    multicolor    super    transcription    endoplasmic    subcellular    sensors    methods    regulate    encompasses    switching    biochemical    nitrogen    toxic    hour    switch    migrates    specimens    takes    protease    stress    physiological    tracking    resolution    cellular    single   

Project "HDPROBES" data sheet

The following table provides information about the project.

Coordinator
ECOLE POLYTECHNIQUE FEDERALE DE LAUSANNE 

Organization address
address: BATIMENT CE 3316 STATION 1
city: LAUSANNE
postcode: 1015
website: www.epfl.ch

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Switzerland [CH]
 Total cost 1˙498˙125 €
 EC max contribution 1˙498˙125 € (100%)
 Programme 1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC))
 Code Call ERC-2018-STG
 Funding Scheme ERC-STG
 Starting year 2019
 Duration (year-month-day) from 2019-10-01   to  2024-09-30

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    ECOLE POLYTECHNIQUE FEDERALE DE LAUSANNE CH (LAUSANNE) coordinator 1˙498˙125.00

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 Project objective

In this proposal, we introduce two new families of probes for live-cell super-resolution microscopy. The first class comprises small-molecule fluorescent sensors for detecting short-lived, small signaling molecules and active enzymes with single-molecule resolution. The spatiotemporal confinement of biological reactive molecules has been hypothesized to regulate various pathological and physiological processes, but the lack of tools to observe directly these microdomains of biochemical activity has precluded the investigation of these mechanisms. The ability to detect small signaling agents and active enzymes with nanometric resolution in intact live specimens will allow us to study the role of compartmentalization in intracellular signaling at an unprecedented resolution. Our studies will focus on detecting elusive reactive oxygen and nitrogen species directly at their sites of endogenous production. We will also investigate the subcellular distribution of protease activity, focusing on its role in non-apoptotic signaling. The second class of probes encompasses a palette of fluorescent dyes that switch continuously between dark and emissive forms. This dynamic equilibrium will enable the localization of single molecules in a densely labeled field without the need to apply toxic light for photoactivation. Based on a novel switching mechanism, we will prepare dyes of various emission wavelengths that blink in a controlled way. These dyes will allow us to perform, for the first time, super-resolution, multicolor, time-lapse imaging of live specimens over long time. Initial studies will focus on tracking a transcription factor that migrates from the endoplasmic reticulum to the nucleus to initiate a cellular stress response upon protein misfolding. These studies will provide spatiotemporal details of this important translocation, which takes more than one hour to occur and its observation at the single-molecule level is intractable with current super-resolution methods

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The information about "HDPROBES" are provided by the European Opendata Portal: CORDIS opendata.

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