EU H2020 Project "CRYO-H-REC (Cryo-EM Imaging of Histone Recycling at the Replication Fork)": description, partecipants, costs and EC-fundings

#	Pagina
attuale pagina	/open-h2020/projects/221623/index.html

Cryo-H-Rec project word cloud

Explore the words cloud of the Cryo-H-Rec project. It provides you a very rough idea of what is the project "Cryo-H-Rec" about.

strands post array modifications dismantles nmr densely pivotal solution duplicated intermediates controls molecular redeposition density histones reassembles template incorporation marks image subject describe unravel chromosome resort seek synthesized disassembly silencing intend nucleosome ptms spectrometry underpins genetic employ techniques nascent material arrays protects cells mass histone crosslinking perform multidisciplinary activation replication proteins expression packaged vitro modulates translational ahead capture chromatin em epigenetic recycling daughter electron structural duplication proliferation maintaining reshuffling mechanism replisome organisation reconstitution newly inheritance eukaryotic regulating fork duplicate intermediate gene transmission chromosomal ms dissect dna machinery components spectroscopy regions complementary chromatinised characterise microscopy cell parental code nucleosomes concerted elucidate form cryo xl

Project "Cryo-H-Rec" data sheet

The following table provides information about the project.

Coordinator	THE FRANCIS CRICK INSTITUTE LIMITED Organization address address: 1 MIDLAND ROAD city: LONDON postcode: NW1 1AT website: www.crick.ac.uk contact info title: n.a. name: n.a. surname: n.a. function: n.a. email: n.a. telephone: n.a. fax: n.a.
Coordinator Country	United Kingdom [UK]
Total cost	212˙933 €
EC max contribution	212˙933 € (100%)
Programme	1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
Code Call	H2020-MSCA-IF-2018
Funding Scheme	MSCA-IF-EF-ST
Starting year	2019
Duration (year-month-day)	from 2019-04-01 to 2021-03-31

#	participants	country	role	EC contrib. [€]
1	THE FRANCIS CRICK INSTITUTE LIMITED	UK (LONDON)	coordinator	212˙933.00

THE FRANCIS CRICK INSTITUTE LIMITED

UK (LONDON)

coordinator

212˙933.00

Project objective

DNA replication is essential for cell proliferation. In eukaryotic cells, DNA is densely packaged in nucleosome arrays that form chromatin. Such organisation protects the genetic material and controls access to DNA, thus providing an important mechanism for regulating gene expression. The eukaryotic replication machinery has evolved to unravel nucleosomes in order to access and duplicate DNA, while also maintaining chromatin density on newly duplicated DNA. To achieve this, the replisome dismantles nucleosomes ahead of the replication fork and reassembles them on nascent DNA strands, by coordinating redeposition of parental and newly synthesized histones. Histone proteins are subject to an array of post-translational modifications (PTMs), providing an epigenetic code that modulates activation and silencing of specific chromosomal regions. Redeposition of parental histones with their PTMs on both nascent DNA strands is, thus, pivotal in transmission of the epigenetic marks to daughter cells. I intend to perform in vitro reconstitution of the replisome on a chromatinised template and use cryo-electron microscopy to image DNA duplication and parental histone recycling at the replication fork. I seek to describe different structural intermediates in the process of nucleosome disassembly, DNA duplication and histone incorporation into new nucleosomes. To capture and characterise intermediate states of the replication machinery during this concerted process, I will employ a multidisciplinary approach and resort to structural techniques complementary to cryo-EM like solution NMR spectroscopy and crosslinking-mass spectrometry (XL-MS). My results will help dissect the role of different replisome components in nucleosome reshuffling at the replication fork, and elucidate the molecular mechanism that underpins chromosome replication and epigenetic inheritance.

Are you the coordinator (or a participant) of this project? Plaese send me more information about the "CRYO-H-REC" project.

For instance: the website url (it has not provided by EU-opendata yet), the logo, a more detailed description of the project (in plain text as a rtf file or a word file), some pictures (as picture files, not embedded into any word file), twitter account, linkedin page, etc.

Send me an email (fabio@fabiodisconzi.com) and I put them in your project's page as son as possible.

Thanks. And then put a link of this page into your project's website.

The information about "CRYO-H-REC" are provided by the European Opendata Portal: CORDIS opendata.

More projects from the same programme (H2020-EU.1.3.2.)