Opendata, web and dolomites

DynamicAssemblies SIGNED

Conformational studies of highly dynamic viral replication complexes

Total Cost €

0

EC-Contrib. €

0

Partnership

0

Views

0

 DynamicAssemblies project word cloud

Explore the words cloud of the DynamicAssemblies project. It provides you a very rough idea of what is the project "DynamicAssemblies" about.

membraneless    disordered    unfolded    elaboration    exploited    phosphoprotein    normally    regulatory    dynamics    modification    fluorescence    combined    cellular    rendered    strand    molecular    translational    cycle    tools    saxs    chemical    physical    express    unexplained    spectroscopy    pathogens    previously    describe    nucleocapsids    genomes    regions    transcription    genetic    structure    resolution    conformational    mechanisms    measles    tetrameric    simulation    host    descriptions    newly    trajectories    nmr    extensive    atomic    intrinsically    cryoem    initiating    machinery    organelles    dangerous    parsimoniously    observation    disorder    engineering    basis    soluble    viruses    nucleoprotein    requiring    intrinsic    solution    droplets    assembly    flexibility    ongoing    kinetics    chaperoning    revolutionizing    enigmatic    proteins    mixed    interaction    resolved    synthesized    sites    biology    functional    post    poorly    human    crystallography    paramyxoviruses    exhibit    replication    assemble    understand    inaccessible    ncs    negative    viral    form    interactions    complexes    liquid    time    rna    extremely   

Project "DynamicAssemblies" data sheet

The following table provides information about the project.

Coordinator
COMMISSARIAT A L ENERGIE ATOMIQUE ET AUX ENERGIES ALTERNATIVES 

Organization address
address: RUE LEBLANC 25
city: PARIS 15
postcode: 75015
website: www.cea.fr

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country France [FR]
 Total cost 2˙499˙150 €
 EC max contribution 2˙499˙150 € (100%)
 Programme 1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC))
 Code Call ERC-2018-ADG
 Funding Scheme ERC-ADG
 Starting year 2019
 Duration (year-month-day) from 2019-11-01   to  2024-10-31

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    COMMISSARIAT A L ENERGIE ATOMIQUE ET AUX ENERGIES ALTERNATIVES FR (PARIS 15) coordinator 2˙499˙150.00

Map

 Project objective

Paramyxoviruses, including, measles and a number of dangerous human pathogens, are negative strand RNA viruses that express their own machinery for transcription and replication. Different interactions between the nucleoprotein (N) and the phosphoprotein (P) are essential for chaperoning and assembly of N on newly synthesized RNA genomes to form nucleocapsids (NCs), as well as for initiating replication and transcription. Both N and tetrameric P exhibit extensive conformational disorder, with very long, unfolded regions that host important post-translational modification sites as well as regulatory interactions with host and viral partners. The presence of this level of disorder, in viruses whose genetic information is normally so parsimoniously exploited, remains unexplained. The elaboration of time-resolved, atomic resolution descriptions of the interaction trajectories of these highly disordered N:P complexes is extremely challenging, requiring the development of adapted methodologies that can account for their intrinsic flexibility. The role of N and P has been rendered yet more enigmatic following our recent observation that when mixed in solution they form liquid-like droplets. Such membraneless organelles are revolutionizing our understanding of cellular chemical biology, although their physical basis is poorly understood. Our aim is to describe these important complexes at atomic resolution, in particular to understand the role of the extensive conformational dynamics of N and P in the replication cycle. Our recent success in engineering soluble N:P complexes from measles that assemble into NCs, combined with ongoing development of NMR-based methods to investigate the structure, dynamics and interaction kinetics of large, intrinsically disordered proteins, fluorescence spectroscopy, cryoEM, SAXS, crystallography and molecular simulation, will provide the essential tools to investigate the functional mechanisms of these previously inaccessible complexes.

Are you the coordinator (or a participant) of this project? Plaese send me more information about the "DYNAMICASSEMBLIES" project.

For instance: the website url (it has not provided by EU-opendata yet), the logo, a more detailed description of the project (in plain text as a rtf file or a word file), some pictures (as picture files, not embedded into any word file), twitter account, linkedin page, etc.

Send me an  email (fabio@fabiodisconzi.com) and I put them in your project's page as son as possible.

Thanks. And then put a link of this page into your project's website.

The information about "DYNAMICASSEMBLIES" are provided by the European Opendata Portal: CORDIS opendata.

More projects from the same programme (H2020-EU.1.1.)

NanoPD_P (2020)

High throughput multiplexed trace-analyte screening for diagnostics applications

Read More  

CARBOFLOW (2020)

Streamlined carbon dioxide conversion in ionic liquids – a platform strategy for modern carbonylation chemistry

Read More  

SUExp (2018)

Strategic Uncertainty: An Experimental Investigation

Read More