Opendata, web and dolomites

CRISPRcombo SIGNED

Interrogating native CRISPR arrays to achieve scalable combinatorial screens and dissect genetic redundancy

Total Cost €

0

EC-Contrib. €

0

Partnership

0

Views

0

 CRISPRcombo project word cloud

Explore the words cloud of the CRISPRcombo project. It provides you a very rough idea of what is the project "CRISPRcombo" about.

core    pot    perform    throughput    redundancy    ensuing    combinatorial    scalable    genetic    posit    pervading    untangling    opportunity    coli    capability    unexplored    small    libraries    crispr    components    assembly    memory    processed    leap    highlighted    theme    compact    inform    cas    construction    intended    ubiquitous    screens    seemingly    techniques    reveal    arrays    active    grants    time    web    gene    modular    conventional    pathogenesis    successful    poised    array    readily    first    adoption    elucidate    naturally    transcript    abundant    genetically    faced    interrogating    constraints    assembled    single    harnessing    uniformly    multiple    yielding    dissecting    hampered    hundreds    grnas    parallel    rules    designing    breakthrough    interactions    made    guide    shared    drive    underexplored    repetitive    virtually    limitations    stable    impossible    propensity    exceeded    native    encode    group    multiplexing    rnas    immunological    poorly    form    biology    technologies    compelling    equivalent   

Project "CRISPRcombo" data sheet

The following table provides information about the project.

Coordinator
HELMHOLTZ-ZENTRUM FUR INFEKTIONSFORSCHUNG GMBH 

Organization address
address: INHOFFENSTRASSE 7
city: BRAUNSCHWEIG
postcode: 38124
website: www.helmholtz-hzi.de

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Germany [DE]
 Total cost 2˙000˙000 €
 EC max contribution 2˙000˙000 € (100%)
 Programme 1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC))
 Code Call ERC-2019-COG
 Funding Scheme ERC-COG
 Starting year 2020
 Duration (year-month-day) from 2020-06-01   to  2025-05-31

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    HELMHOLTZ-ZENTRUM FUR INFEKTIONSFORSCHUNG GMBH DE (BRAUNSCHWEIG) coordinator 2˙000˙000.00

Map

 Project objective

A ubiquitous yet poorly understood theme pervading biology is redundancy, wherein seemingly equivalent components drive shared processes. In cases from development to pathogenesis, untangling the ensuing web of potential genetic interactions can be virtually impossible with conventional techniques. CRISPR technologies, with their propensity for multiplexing, are well poised to address this challenge. However, current CRISPR-based screens have not exceeded more than two targets at a time. Here, I will achieve a major leap forward for CRISPR-based screens and dissecting redundancy by harnessing a core yet underexplored part of CRISPR: CRISPR arrays. CRISPR arrays naturally form the immunological memory of CRISPR-Cas systems and produce multiple targeting gRNAs processed from a single transcript. The arrays are highly compact, genetically stable, and can encode hundreds of gRNAs. However, the repetitive “repeats” within each array have hampered their construction and widespread adoption. My group recently made a breakthrough with the modular one-pot assembly of long arrays and array libraries. This capability grants us the unique opportunity to develop the first high-throughput, CRISPR-based screens that readily scale to many gene targets at a time. In parallel, our first assembled arrays highlighted technical constraints to designing robust and highly active arrays. I posit that native CRISPR arrays have faced similar limitations and thus can inform the design of array libraries. I thus propose to 1) Develop design rules for CRISPR arrays yielding only intended and uniformly abundant guide RNAs. 2) Elucidate and exploit why CRISPR arrays are genetically stable. 3) Perform scalable combinatorial screens using redundancy by small RNAs in E. coli as a compelling case study. If successful, this project will reveal unexplored properties of CRISPR arrays and, for the first time, achieve scalable combinatorial screens for interrogating redundancy throughout biology.

Are you the coordinator (or a participant) of this project? Plaese send me more information about the "CRISPRCOMBO" project.

For instance: the website url (it has not provided by EU-opendata yet), the logo, a more detailed description of the project (in plain text as a rtf file or a word file), some pictures (as picture files, not embedded into any word file), twitter account, linkedin page, etc.

Send me an  email (fabio@fabiodisconzi.com) and I put them in your project's page as son as possible.

Thanks. And then put a link of this page into your project's website.

The information about "CRISPRCOMBO" are provided by the European Opendata Portal: CORDIS opendata.

More projects from the same programme (H2020-EU.1.1.)

ENUF (2019)

Evaluation of Novel Ultra-Fast selective III-V Epitaxy

Read More  

Aware (2019)

Aiding Antibiotic Development with Deep Analysis of Resistance Evolution

Read More  

TAMING CORROSION (2020)

Towards mastering the long-standing challenge of ageing infrastructures in corrosive environments

Read More