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ICaRMuSs SIGNED

Intragranullar calcium regulation of mucin secretion and sorting.

Total Cost €

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EC-Contrib. €

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Partnership

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 ICaRMuSs project word cloud

Explore the words cloud of the ICaRMuSs project. It provides you a very rough idea of what is the project "ICaRMuSs" about.

pulmonary    infections    plasma    synthesized    insults    mucus    crispr    intragranular    training    abnormalities    spectrometry    gap    dysfunction    encountered    signal    cystic    composition    speculation    tools    biology    purify    goblet    inflammation    secretion    granular    describe    defects    granules    cas9    secreting    track    probe    physiology    sequence    disease    layer    golgi    engineer    obstructive    continuous    cells    innovative    proteins    me    questions    space    protects    creative    trafficking    facs    adapt    gastrointestinal    fusion    mobility    concentration    genetically    sorting    gfp    dictated    asthma    mass    heavily    add    rfp    membrane    airways    pathologies    extracellular    gaps    international    calcium    first    secretory    epithelium    largely    muc5ac    covered    mucin    aggressive    triggers    microbial    cell    apparatus    microscopy    deleterious    fibrosis    super    mayor    precisely    abundant    mechanical    resolution    secreted    muc2    mucins    modify    vesicles    stress    epithelial    releasing    packed    regulates    function    glysosylated    filling    chronic    loci   

Project "ICaRMuSs" data sheet

The following table provides information about the project.

Coordinator
FUNDACIO CENTRE DE REGULACIO GENOMICA 

Organization address
address: CARRER DOCTOR AIGUADER 88
city: BARCELONA
postcode: 8003
website: www.crg.es

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Spain [ES]
 Total cost 172˙932 €
 EC max contribution 172˙932 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2019
 Funding Scheme MSCA-IF-EF-ST
 Starting year 2020
 Duration (year-month-day) from 2020-04-01   to  2022-03-31

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    FUNDACIO CENTRE DE REGULACIO GENOMICA ES (BARCELONA) coordinator 172˙932.00

Map

 Project objective

The epithelium in the airways and gastrointestinal track are covered by a mucus layer which protects the epithelium from mechanical and microbial insults. The most abundant proteins in the mucus layer are mucins which are synthesized and secreted by goblet cells. Mucus function is largely dictated by mucin composition and goblet cells can modify their secretory properties to adapt to the continuous changes the epithelium is encountered with. Mucins are heavily glysosylated and packed into secretory vesicles in the Golgi apparatus. A calcium signal triggers the fusion of the secretory vesicles with the plasma membrane releasing mucins to the extracellular space. The role of intragranular calcium remains a mayor gap in our understanding of mucin physiology and how goblet cells precisely control mucus composition remains only a matter of speculation. These are highly relevant questions as defects in mucin secretion cause mucus abnormalities, epithelial stress and dysfunction that can lead to microbial deleterious infections, chronic inflammation and more aggressive pathologies such as cystic fibrosis, asthma, and chronic obstructive pulmonary disease. In this project I will address how calcium regulates the trafficking, sorting and secretion of mucins, filling current gaps in our understanding of mucus physiology. I will genetically engineer mucin-secreting cells with the CRISPR/Cas9 tools to add GFP and RFP to MUC5AC and MUC2 loci and describe granular composition by super resolution microscopy and FACS. I will use a first-in its kind probe to measure intragranular calcium concentration and finally I will purify secretory granules and sequence them with mass spectrometry to determine which membrane proteins are important for mucin secretion. This project will allow me to apply my experience in highly relevant questions in cell biology and it will also enhance my creative and innovative potential through advanced training and international mobility.

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The information about "ICARMUSS" are provided by the European Opendata Portal: CORDIS opendata.

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relatedResult rcn (863570) AGGIORNATO correttamente
lastchecktime (2022-10-01 2:21:10) correctly updated