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ICaRMuSs SIGNED

Intragranullar calcium regulation of mucin secretion and sorting.

Total Cost €

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EC-Contrib. €

0

Partnership

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 ICaRMuSs project word cloud

Explore the words cloud of the ICaRMuSs project. It provides you a very rough idea of what is the project "ICaRMuSs" about.

questions    cells    mobility    secreting    triggers    cell    innovative    resolution    probe    engineer    obstructive    purify    airways    secretion    rfp    secretory    mucin    microbial    chronic    filling    heavily    fusion    granular    asthma    intragranular    packed    mucins    facs    inflammation    concentration    infections    sorting    add    genetically    muc5ac    biology    cystic    largely    golgi    crispr    encountered    proteins    modify    creative    plasma    pathologies    loci    insults    speculation    gastrointestinal    trafficking    precisely    calcium    pulmonary    disease    granules    function    gfp    dysfunction    international    synthesized    covered    mechanical    gaps    releasing    physiology    sequence    aggressive    membrane    abundant    mayor    protects    continuous    tools    gap    signal    deleterious    abnormalities    super    me    space    epithelium    mucus    extracellular    glysosylated    spectrometry    layer    adapt    goblet    describe    training    mass    apparatus    defects    track    first    muc2    stress    vesicles    composition    cas9    secreted    dictated    epithelial    fibrosis    regulates    microscopy   

Project "ICaRMuSs" data sheet

The following table provides information about the project.

Coordinator
FUNDACIO CENTRE DE REGULACIO GENOMICA 

Organization address
address: CARRER DOCTOR AIGUADER 88
city: BARCELONA
postcode: 8003
website: www.crg.es

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Spain [ES]
 Total cost 172˙932 €
 EC max contribution 172˙932 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2019
 Funding Scheme MSCA-IF-EF-ST
 Starting year 2020
 Duration (year-month-day) from 2020-04-01   to  2022-03-31

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    FUNDACIO CENTRE DE REGULACIO GENOMICA ES (BARCELONA) coordinator 172˙932.00

Map

 Project objective

The epithelium in the airways and gastrointestinal track are covered by a mucus layer which protects the epithelium from mechanical and microbial insults. The most abundant proteins in the mucus layer are mucins which are synthesized and secreted by goblet cells. Mucus function is largely dictated by mucin composition and goblet cells can modify their secretory properties to adapt to the continuous changes the epithelium is encountered with. Mucins are heavily glysosylated and packed into secretory vesicles in the Golgi apparatus. A calcium signal triggers the fusion of the secretory vesicles with the plasma membrane releasing mucins to the extracellular space. The role of intragranular calcium remains a mayor gap in our understanding of mucin physiology and how goblet cells precisely control mucus composition remains only a matter of speculation. These are highly relevant questions as defects in mucin secretion cause mucus abnormalities, epithelial stress and dysfunction that can lead to microbial deleterious infections, chronic inflammation and more aggressive pathologies such as cystic fibrosis, asthma, and chronic obstructive pulmonary disease. In this project I will address how calcium regulates the trafficking, sorting and secretion of mucins, filling current gaps in our understanding of mucus physiology. I will genetically engineer mucin-secreting cells with the CRISPR/Cas9 tools to add GFP and RFP to MUC5AC and MUC2 loci and describe granular composition by super resolution microscopy and FACS. I will use a first-in its kind probe to measure intragranular calcium concentration and finally I will purify secretory granules and sequence them with mass spectrometry to determine which membrane proteins are important for mucin secretion. This project will allow me to apply my experience in highly relevant questions in cell biology and it will also enhance my creative and innovative potential through advanced training and international mobility.

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The information about "ICARMUSS" are provided by the European Opendata Portal: CORDIS opendata.

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