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ICaRMuSs SIGNED

Intragranullar calcium regulation of mucin secretion and sorting.

Total Cost €

0

EC-Contrib. €

0

Partnership

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 ICaRMuSs project word cloud

Explore the words cloud of the ICaRMuSs project. It provides you a very rough idea of what is the project "ICaRMuSs" about.

glysosylated    cas9    membrane    calcium    tools    defects    physiology    mucin    secretion    mobility    track    gap    inflammation    pulmonary    first    triggers    synthesized    apparatus    cystic    granules    space    gfp    dictated    questions    facs    describe    biology    mechanical    signal    goblet    filling    probe    microscopy    composition    dysfunction    aggressive    proteins    extracellular    mayor    stress    layer    cell    disease    add    intragranular    mass    insults    abundant    covered    purify    encountered    creative    precisely    secreted    gastrointestinal    chronic    secretory    deleterious    obstructive    modify    speculation    sorting    concentration    spectrometry    me    fibrosis    plasma    largely    mucins    trafficking    mucus    abnormalities    releasing    epithelium    granular    packed    vesicles    cells    epithelial    rfp    function    protects    microbial    asthma    international    heavily    continuous    training    muc5ac    innovative    resolution    super    muc2    engineer    genetically    gaps    infections    crispr    pathologies    fusion    secreting    loci    regulates    adapt    sequence    golgi    airways   

Project "ICaRMuSs" data sheet

The following table provides information about the project.

Coordinator
FUNDACIO CENTRE DE REGULACIO GENOMICA 

Organization address
address: CARRER DOCTOR AIGUADER 88
city: BARCELONA
postcode: 8003
website: www.crg.es

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Spain [ES]
 Total cost 172˙932 €
 EC max contribution 172˙932 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2019
 Funding Scheme MSCA-IF-EF-ST
 Starting year 2020
 Duration (year-month-day) from 2020-04-01   to  2022-03-31

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    FUNDACIO CENTRE DE REGULACIO GENOMICA ES (BARCELONA) coordinator 172˙932.00

Map

 Project objective

The epithelium in the airways and gastrointestinal track are covered by a mucus layer which protects the epithelium from mechanical and microbial insults. The most abundant proteins in the mucus layer are mucins which are synthesized and secreted by goblet cells. Mucus function is largely dictated by mucin composition and goblet cells can modify their secretory properties to adapt to the continuous changes the epithelium is encountered with. Mucins are heavily glysosylated and packed into secretory vesicles in the Golgi apparatus. A calcium signal triggers the fusion of the secretory vesicles with the plasma membrane releasing mucins to the extracellular space. The role of intragranular calcium remains a mayor gap in our understanding of mucin physiology and how goblet cells precisely control mucus composition remains only a matter of speculation. These are highly relevant questions as defects in mucin secretion cause mucus abnormalities, epithelial stress and dysfunction that can lead to microbial deleterious infections, chronic inflammation and more aggressive pathologies such as cystic fibrosis, asthma, and chronic obstructive pulmonary disease. In this project I will address how calcium regulates the trafficking, sorting and secretion of mucins, filling current gaps in our understanding of mucus physiology. I will genetically engineer mucin-secreting cells with the CRISPR/Cas9 tools to add GFP and RFP to MUC5AC and MUC2 loci and describe granular composition by super resolution microscopy and FACS. I will use a first-in its kind probe to measure intragranular calcium concentration and finally I will purify secretory granules and sequence them with mass spectrometry to determine which membrane proteins are important for mucin secretion. This project will allow me to apply my experience in highly relevant questions in cell biology and it will also enhance my creative and innovative potential through advanced training and international mobility.

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The information about "ICARMUSS" are provided by the European Opendata Portal: CORDIS opendata.

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