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ICaRMuSs SIGNED

Intragranullar calcium regulation of mucin secretion and sorting.

Total Cost €

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EC-Contrib. €

0

Partnership

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 ICaRMuSs project word cloud

Explore the words cloud of the ICaRMuSs project. It provides you a very rough idea of what is the project "ICaRMuSs" about.

disease    defects    mucin    secretion    microscopy    describe    secreting    secretory    super    gastrointestinal    international    mass    signal    sorting    mucins    mobility    microbial    physiology    dictated    granules    track    questions    membrane    intragranular    mucus    modify    infections    extracellular    add    golgi    biology    glysosylated    layer    inflammation    muc2    chronic    concentration    resolution    tools    goblet    cells    cell    innovative    aggressive    facs    creative    encountered    space    purify    gap    proteins    first    calcium    cas9    pathologies    covered    genetically    spectrometry    vesicles    stress    muc5ac    synthesized    mechanical    speculation    loci    releasing    cystic    filling    probe    abundant    fibrosis    sequence    gfp    airways    heavily    adapt    protects    me    gaps    epithelial    deleterious    dysfunction    crispr    training    composition    mayor    abnormalities    apparatus    trafficking    fusion    continuous    epithelium    regulates    secreted    rfp    obstructive    pulmonary    function    packed    triggers    insults    precisely    largely    granular    asthma    plasma    engineer   

Project "ICaRMuSs" data sheet

The following table provides information about the project.

Coordinator
FUNDACIO CENTRE DE REGULACIO GENOMICA 

Organization address
address: CARRER DOCTOR AIGUADER 88
city: BARCELONA
postcode: 8003
website: www.crg.es

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Spain [ES]
 Total cost 172˙932 €
 EC max contribution 172˙932 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2019
 Funding Scheme MSCA-IF-EF-ST
 Starting year 2020
 Duration (year-month-day) from 2020-04-01   to  2022-03-31

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    FUNDACIO CENTRE DE REGULACIO GENOMICA ES (BARCELONA) coordinator 172˙932.00

Map

 Project objective

The epithelium in the airways and gastrointestinal track are covered by a mucus layer which protects the epithelium from mechanical and microbial insults. The most abundant proteins in the mucus layer are mucins which are synthesized and secreted by goblet cells. Mucus function is largely dictated by mucin composition and goblet cells can modify their secretory properties to adapt to the continuous changes the epithelium is encountered with. Mucins are heavily glysosylated and packed into secretory vesicles in the Golgi apparatus. A calcium signal triggers the fusion of the secretory vesicles with the plasma membrane releasing mucins to the extracellular space. The role of intragranular calcium remains a mayor gap in our understanding of mucin physiology and how goblet cells precisely control mucus composition remains only a matter of speculation. These are highly relevant questions as defects in mucin secretion cause mucus abnormalities, epithelial stress and dysfunction that can lead to microbial deleterious infections, chronic inflammation and more aggressive pathologies such as cystic fibrosis, asthma, and chronic obstructive pulmonary disease. In this project I will address how calcium regulates the trafficking, sorting and secretion of mucins, filling current gaps in our understanding of mucus physiology. I will genetically engineer mucin-secreting cells with the CRISPR/Cas9 tools to add GFP and RFP to MUC5AC and MUC2 loci and describe granular composition by super resolution microscopy and FACS. I will use a first-in its kind probe to measure intragranular calcium concentration and finally I will purify secretory granules and sequence them with mass spectrometry to determine which membrane proteins are important for mucin secretion. This project will allow me to apply my experience in highly relevant questions in cell biology and it will also enhance my creative and innovative potential through advanced training and international mobility.

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The information about "ICARMUSS" are provided by the European Opendata Portal: CORDIS opendata.

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lastchecktime (2022-05-16 9:03:18) correctly updated