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ICaRMuSs SIGNED

Intragranullar calcium regulation of mucin secretion and sorting.

Total Cost €

0

EC-Contrib. €

0

Partnership

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 ICaRMuSs project word cloud

Explore the words cloud of the ICaRMuSs project. It provides you a very rough idea of what is the project "ICaRMuSs" about.

asthma    describe    fusion    pathologies    vesicles    intragranular    secreted    defects    abundant    obstructive    secretion    mass    precisely    granules    dictated    proteins    concentration    regulates    membrane    spectrometry    questions    modify    trafficking    gastrointestinal    speculation    secretory    secreting    dysfunction    encountered    sorting    genetically    covered    largely    first    muc5ac    microbial    me    tools    abnormalities    probe    filling    super    granular    mobility    mucus    international    synthesized    muc2    disease    mucin    mucins    packed    aggressive    triggers    fibrosis    continuous    gap    heavily    goblet    mechanical    gfp    function    cas9    facs    loci    adapt    extracellular    mayor    stress    purify    apparatus    cells    insults    track    epithelium    deleterious    training    glysosylated    gaps    releasing    inflammation    chronic    physiology    crispr    microscopy    protects    composition    infections    creative    plasma    sequence    cell    cystic    biology    resolution    golgi    innovative    layer    pulmonary    space    signal    engineer    airways    calcium    epithelial    rfp    add   

Project "ICaRMuSs" data sheet

The following table provides information about the project.

Coordinator
FUNDACIO CENTRE DE REGULACIO GENOMICA 

Organization address
address: CARRER DOCTOR AIGUADER 88
city: BARCELONA
postcode: 8003
website: www.crg.es

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Spain [ES]
 Total cost 172˙932 €
 EC max contribution 172˙932 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2019
 Funding Scheme MSCA-IF-EF-ST
 Starting year 2020
 Duration (year-month-day) from 2020-04-01   to  2022-03-31

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    FUNDACIO CENTRE DE REGULACIO GENOMICA ES (BARCELONA) coordinator 172˙932.00

Map

 Project objective

The epithelium in the airways and gastrointestinal track are covered by a mucus layer which protects the epithelium from mechanical and microbial insults. The most abundant proteins in the mucus layer are mucins which are synthesized and secreted by goblet cells. Mucus function is largely dictated by mucin composition and goblet cells can modify their secretory properties to adapt to the continuous changes the epithelium is encountered with. Mucins are heavily glysosylated and packed into secretory vesicles in the Golgi apparatus. A calcium signal triggers the fusion of the secretory vesicles with the plasma membrane releasing mucins to the extracellular space. The role of intragranular calcium remains a mayor gap in our understanding of mucin physiology and how goblet cells precisely control mucus composition remains only a matter of speculation. These are highly relevant questions as defects in mucin secretion cause mucus abnormalities, epithelial stress and dysfunction that can lead to microbial deleterious infections, chronic inflammation and more aggressive pathologies such as cystic fibrosis, asthma, and chronic obstructive pulmonary disease. In this project I will address how calcium regulates the trafficking, sorting and secretion of mucins, filling current gaps in our understanding of mucus physiology. I will genetically engineer mucin-secreting cells with the CRISPR/Cas9 tools to add GFP and RFP to MUC5AC and MUC2 loci and describe granular composition by super resolution microscopy and FACS. I will use a first-in its kind probe to measure intragranular calcium concentration and finally I will purify secretory granules and sequence them with mass spectrometry to determine which membrane proteins are important for mucin secretion. This project will allow me to apply my experience in highly relevant questions in cell biology and it will also enhance my creative and innovative potential through advanced training and international mobility.

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The information about "ICARMUSS" are provided by the European Opendata Portal: CORDIS opendata.

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