Explore the words cloud of the SM-SPAD project. It provides you a very rough idea of what is the project "SM-SPAD" about.
The following table provides information about the project.
FONDAZIONE ISTITUTO ITALIANO DI TECNOLOGIA
|Coordinator Country||Italy [IT]|
|Total cost||171˙473 €|
|EC max contribution||171˙473 € (100%)|
1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
|Duration (year-month-day)||from 2021-03-01 to 2023-02-28|
Take a look of project's partnership.
|1||FONDAZIONE ISTITUTO ITALIANO DI TECNOLOGIA||IT (GENOVA)||coordinator||171˙473.00|
The resolution limit of about 250 nm in conventional optical microscopes is problematic in the study of structural biology, since proteins, macromolecules and nuclear acids are typically much smaller than 100 nm. Single-molecule localization microscopy is able to circumvent this limit by sequentially and stochastically switching on/activating single fluorescent molecules and determining their position in the image plane. E.g. MINFLUX demonstrated a 3D resolution of 6 nm. However, the point-detection system in MINFLUX does not allow directly recording the image of the fluorescent molecules in the image plane, which is required for the localization, thus a rather complex and slow beam-scanning approach is required. Secondly, the technique does not leverage the fluorescence lifetime information, which can provide nanometer-scale information on the structure of interest, e.g. via Förster resonance energy transfer. In this project, both limitations will be tackled:
The main goal of the SM-SPAD project is to develop a 3D single-molecule fluorescence lifetime imaging technique for structural biology. The goal will be reached by combining the concept of MINFLUX with three new ideas: (i) 3D motionless structured illumination and structured detection for improved spatial resolution in all three dimensions; (ii) fluorescence antibunching analysis to speed up the data acquisition by enabling simultaneous localization of several active molecules; (iii) SM level fluorescence lifetime analysis to extract the maximum amount of information from the sample.
The proposed molecular-scale imaging technique, with very high spatial and temporal resolution, is perfectly suited for the study of complex biological samples. SM-SPAD is, in particular, promising in the field of neuroscience research, in which the organization of large protein complexes are of high interest, as they may play a crucial role in the development of neurodegenerative diseases, such as Alzheimer’s and Parkinson’s .
Are you the coordinator (or a participant) of this project? Plaese send me more information about the "SM-SPAD" project.
For instance: the website url (it has not provided by EU-opendata yet), the logo, a more detailed description of the project (in plain text as a rtf file or a word file), some pictures (as picture files, not embedded into any word file), twitter account, linkedin page, etc.
Send me an email (firstname.lastname@example.org) and I put them in your project's page as son as possible.
Thanks. And then put a link of this page into your project's website.
The information about "SM-SPAD" are provided by the European Opendata Portal: CORDIS opendata.
Neural mechanisms of crossmodal activity in blind and sighted individualsRead More
The Canadian model of the public-private sponsorship for the integration of refugees: the case of Syrians and possible application in EU countriesRead More
Multifunctional Immunocompatible NanoTheranostics to modulate tumor microenvironment and improve treatment monitoring: A double blow to pancreatic cancerRead More