SAVVY

Self-assembled virus-like vectors for stem cell phenotyping

 Coordinatore Karlsruher Institut fuer Technologie 

 Organization address address: Kaiserstrasse 12
city: Karlsruhe
postcode: 76131

contact info
Titolo: Ms.
Nome: Natascha
Cognome: Kindsvogel
Email: send email
Telefono: +49 721 608 25414
Fax: +49 721 608 25403

 Nazionalità Coordinatore Germany [DE]
 Totale costo 4˙857˙066 €
 EC contributo 3˙782˙729 €
 Programma FP7-NMP
Specific Programme "Cooperation": Nanosciences, Nanotechnologies, Materials and new Production Technologies
 Code Call FP7-NMP-2012-SMALL-6
 Funding Scheme CP-FP
 Anno di inizio 2013
 Periodo (anno-mese-giorno) 2013-01-01   -   2015-12-31

 Partecipanti

# participant  country  role  EC contrib. [€] 
1    Karlsruher Institut fuer Technologie

 Organization address address: Kaiserstrasse 12
city: Karlsruhe
postcode: 76131

contact info
Titolo: Ms.
Nome: Natascha
Cognome: Kindsvogel
Email: send email
Telefono: +49 721 608 25414
Fax: +49 721 608 25403

DE (Karlsruhe) coordinator 1˙067˙915.08
2    IMPERIAL COLLEGE OF SCIENCE, TECHNOLOGY AND MEDICINE

 Organization address address: SOUTH KENSINGTON CAMPUS EXHIBITION ROAD
city: LONDON
postcode: SW7 2AZ

contact info
Titolo: Mr.
Nome: Shaun
Cognome: Power
Email: send email
Telefono: +44 207 594 8773
Fax: +44 207 594 8609

UK (LONDON) participant 788˙045.20
3    MICROFLUIDIC CHIPSHOP GMBH

 Organization address address: STOCKHOLMER STRASSE 20
city: JENA
postcode: 7747

contact info
Titolo: Dr.
Nome: Holger
Cognome: Becker
Email: send email
Telefono: +49 3641 347050

DE (JENA) participant 580˙400.00
4    ECOLE POLYTECHNIQUE FEDERALE DE LAUSANNE

 Organization address address: BATIMENT CE 3316 STATION 1
city: LAUSANNE
postcode: 1015

contact info
Titolo: Prof.
Nome: Francesco
Cognome: Stellacci
Email: send email
Telefono: +41 21 693 7872
Fax: +41 21 693 5270

CH (LAUSANNE) participant 509˙714.00
5    ASOCIACION CENTRO DE INVESTIGACION COOPERATIVA EN BIOMATERIALES

 Organization address address: PASEO MIRAMON PARQUE TECNOLOGICO DE SAN SEBASTIAN EDIFICIO EMPRESARIAL C 182
city: SAN SEBASTIAN
postcode: 20009

contact info
Titolo: Mr.
Nome: Alfonso
Cognome: Egaña
Email: send email
Telefono: +34 943005300
Fax: +34 943005301

ES (SAN SEBASTIAN) participant 468˙289.00
6    WITEC WISSENSCHAFTLICHE INSTRUMENTEUND TECHNOLOGIE GMBH

 Organization address address: LISE MEITNER STRASSE 6
city: ULM
postcode: 89081

contact info
Titolo: Dr.
Nome: Joachim
Cognome: Koenen
Email: send email
Telefono: +49 731 14070120

DE (ULM) participant 263˙365.72
7    UNIVERSIDAD DE VIGO

 Organization address address: LG CAMPUS LAGOAS MARCOSENDE
city: VIGO PONTEVEDRA
postcode: 36310

contact info
Titolo: Mr.
Nome: Anxo
Cognome: Moreira González
Email: send email
Telefono: +34 986 812052

ES (VIGO PONTEVEDRA) participant 105˙000.00
8    OMT-OPTISCHE MESSTECHNIK GMBH

 Organization address address: HORVELSINGER WEG 6
city: ULM
postcode: 89081

contact info
Titolo: Dr.
Nome: Nancy
Cognome: Hecker-Denschlag
Email: send email
Telefono: +49 731 159169 0

DE (ULM) participant 0.00

Mappa


 Word cloud

Esplora la "nuvola delle parole (Word Cloud) per avere un'idea di massima del progetto.

free    acceptable    stem    assembled    rippled    savvy    therapeutic    virus    viruses    analogues    biomarkers    sorting    modified    self    uses    signatures    our    populations    technologies    heterogeneous    polymer    markers    surface    differences    fingerprinting    membranes    sort    human    lack    intrinsically    label    types    composition    concentration    nps    dissimilar    peptides    specifically    fluorescence    phenotyping    aptamers    bicompartmental    multifunctional    biotechnological    probes    intrinsic    sers    scientists    membrane    microfluidic    nanoparticles    gold    cell    raman    particles    vectors    scalable    cells    assembly    distinguish    antibodies    relies   

 Obiettivo del progetto (Objective)

'The Self-Assembled Virus-like Vectors for Stem Cell Phenotyping (SAVVY) project relies on hierarchical, multi-scale assembly of intrinsically dissimilar nanoparticles to develop novel types of multifunctional Raman probes for analysis and phenotyping of heterogeneous stem cell populations. Our project will address a large unmet need, as stem cells have great potential for a broad range of therapeutic and biotechnological applications. Characterization and sorting of heterogeneous stem cell populations has been intrinsically hampered by (1) lack of specific antibodies, (2) need for fluorescence markers, (3) low concentration of stem cells, (4) low efficiencies/high costs. Our technology will use a fundamentally different approach that (1) does not require antibodies, aptamers, or biomarkers, (2) is fluorescence-label free, and (3) is scalable at acceptable cost. The approach uses intrinsic differences in the composition of membranes of cells to distinguish cell populations. These differences will be detect by SERS and analysed through multicomponent analysis. We have combined the necessary expertise to tackle this challenge: Stellacci has developed rippled nanoparticles that specifically interact with and adhere to cell membranes (analogues to cell penetrating peptides). Lahann has developed bicompartmental Janus polymer particles that have already been surface-modified with rippled particles and integrate specifically in the cell membrane (analogues to viruses). Liz-Marzan has developed highly Raman-active nanoparticles and has demonstrated their selectivity and specificity in SERS experiments. These Raman probes will be loaded into the synthetic viruses to enable membrane fingerprinting. Stevens has developed a Bioinformatics platform for fingerprinting of stem cell populations using cluster analysis algorithms. The effort will be joined by two SMEs, ChipShop and OMT, that will be able to develop the necessary microfluidic and Raman detection hardware.'

Introduzione (Teaser)

Stem cells have a great potential for many therapeutic and biotechnological applications. An EU-funded project is developing a new approach to sort stem cell populations.

Descrizione progetto (Article)

Characterisation and sorting of stem cell populations is typically performed using flow cytometry. Several factors limit existing sorting technologies, such as lack of specific antibodies, low concentration of stem cells and a need for fluorescence markers.

The http://savvyproject.eu/ (SAVVY) (Self-assembled virus-like vectors for stem cell phenotyping) project is developing a different particle-based approach to cell sorting. The main idea is to use the molecular signatures of heterogeneous stem cell populations to enable their effective separation.

Running for three years to end-2015, the project uses intrinsic differences in the composition of cell membranes to distinguish and ultimately sort stem cell populations. SAVVY's approach does not require antibodies, aptamers or other biomarkers, and it is label-free and scalable at an acceptable cost.

The project relies on multi-scale assembly of intrinsically dissimilar nanoparticles (NPs) to develop novel types of multifunctional Raman probes. The completed live cell sorting system will incorporate NP-based signal enhancers along with Raman microspectroscopy in an integrated microfluidic cell sorter.

To date, the project has produced bicompartmental polymer particles, modified with gold NPs. These SAVVY reporters are currently under investigation concerning their interactions with cell surfaces.

At the same time, a database of Raman signatures of cells is under development. Scientists are performing Raman mapping of human and mouse embryonic stem cells using visible and near-infrared lasers. A first iteration of a Raman signature library was built.

In addition, SAVVY is looking for the surface-enhanced Raman scattering (SERS) signatures of cells. To obtain a model system for the SERS-based phenotyping, scientists applied gold nanostars, functionalised with cell-adhesion peptides, to human neural stem cells and human neurons.

This new approach to phenotyping and sorting will be useful for stem cell-based therapies, tissue regeneration and novel medical technologies. Once validated for stem cell phenotyping, the SAVVY technology may lead to novel diagnostics tools applicable to a number of diseases.

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