GTOXOME

The impact of genotoxic stress on the mRNA-interactome and RNA-regulons

Coordinatore	EUROPEAN MOLECULAR BIOLOGY LABORATORY    more  Organization address address: Meyerhofstrasse 1 city: HEIDELBERG postcode: 69117 contact info Titolo: Ms. Nome: Jillian Cognome: Rowe Email: send email Telefono: 4962210000000 Fax: +49 6221 3878575
Nazionalità Coordinatore	Germany [DE]
Totale costo	161˙968 €
EC contributo	161˙968 €
Programma	FP7-PEOPLE Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013)
Code Call	FP7-PEOPLE-2012-IEF
Funding Scheme	MC-IEF
Anno di inizio	2014
Periodo (anno-mese-giorno)	2014-01-01   -   2015-12-31

 Partecipanti

#	participant	country	role	EC contrib. [€]
1	EUROPEAN MOLECULAR BIOLOGY LABORATORY  Organization address address: Meyerhofstrasse 1 city: HEIDELBERG postcode: 69117 contact info Titolo: Ms. Nome: Jillian Cognome: Rowe Email: send email Telefono: 4962210000000 Fax: +49 6221 3878575	DE (HEIDELBERG)	coordinator	161˙968.80

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 Obiettivo del progetto (Objective)

'RNA-binding proteins (RBP) play critical roles in stress responses like DNA-damage through interactions with elements in functionally related RNAs, termed regulons. One of the most cytotoxic forms of DNA-damage are DNA double strand breaks (DSB), that can lead to mutagenic events and when improperly repaired to cell death. This proposed project focuses on the dynamics of the global RBP network (mRNA-interactome) and RNA-regulons of mouse fibroblasts (NIH-3T3) cells in response to genotoxic stress. The “RNA-regulon” model implies that RBPs control functionally related mRNAs via cis-regulatory elements in these RNAs. Using mRNA-interactome capture and high-resolution quantitative proteomics, I will comprehensively identify mRNA-binding proteins involved in immediate DNA-repair and cell death responses to genotoxic stress. By employing deep sequencing and crosslink immunoprecipitation approaches, I will highlight the controlled RNA-networks and their influence on cell survival. Taken together, this project aims to address key questions of the role for RBPs in the DNA-damage response at different stages and the identification of their RNA-regulons. Furthermore, it opens new perspectives in posttranscriptional regulation after the cells exposure to genotoxic stress. The innovative nature of this interdisciplinary project involves European collaborators, which creates excellent inter-European-networking. The development of a novel research area will showcase the cutting-edge research performed in the ERA thus directly contribute to the European Work Programme by enhancing the attractiveness of Europe for third country and European top-scientists.'