SMD

Single or few molecules detection by combined enhanced spectroscopies

 Coordinatore Consorzio per il Centro di Biomedicina Molecolare S.c.r.l. 

 Organization address address: STRADA STATALE 14 KM 163 5
city: TRIESTE
postcode: 34149

contact info
Titolo: Ms.
Nome: Anna Lucia
Cognome: Bellina
Email: send email
Telefono: +39 0403757713
Fax: +39 0403757710

 Nazionalità Coordinatore Italy [IT]
 Sito del progetto http://www.singlemoleculedetection.eu
 Totale costo 4˙432˙947 €
 EC contributo 3˙421˙500 €
 Programma FP7-NMP
Specific Programme "Cooperation": Nanosciences, Nanotechnologies, Materials and new Production Technologies
 Code Call FP7-NMP-2008-SMALL-2
 Funding Scheme CP-FP
 Anno di inizio 2009
 Periodo (anno-mese-giorno) 2009-07-01   -   2012-06-30

 Partecipanti

# participant  country  role  EC contrib. [€] 
1    Consorzio per il Centro di Biomedicina Molecolare S.c.r.l.

 Organization address address: STRADA STATALE 14 KM 163 5
city: TRIESTE
postcode: 34149

contact info
Titolo: Ms.
Nome: Anna Lucia
Cognome: Bellina
Email: send email
Telefono: +39 0403757713
Fax: +39 0403757710

IT (TRIESTE) coordinator 896˙136.40
2    UNIVERSITY OF STRATHCLYDE

 Organization address address: Richmond Street 16
city: GLASGOW
postcode: G1 1XQ

contact info
Titolo: Mr.
Nome: Martin
Cognome: Gregory
Email: send email
Telefono: +44 1415482524
Fax: +44 1415524409

UK (GLASGOW) participant 499˙232.00
3    RUHR-UNIVERSITAET BOCHUM

 Organization address address: Universitaetstrasse 150
city: BOCHUM
postcode: 44780

contact info
Titolo: Prof.
Nome: Martina
Cognome: Havenith
Email: send email
Telefono: +49 2343224249
Fax: +49 2343214183

DE (BOCHUM) participant 475˙978.00
4    TECHNISCHE UNIVERSITAT DORTMUND

 Organization address address: AUGUST SCHMIDT STRASSE 4
city: DORTMUND
postcode: 44227

contact info
Titolo: Mr.
Nome: Daniel
Cognome: Schneider
Email: send email
Telefono: +49 2317552622
Fax: +49 2317552756

DE (DORTMUND) participant 429˙850.00
5    Nanotec Electronica SL

 Organization address address: RONDA DE PONIENTE 12 2 C
city: TRES CANTOS MADRID
postcode: 28760

contact info
Titolo: Ms.
Nome: Leonor
Cognome: Badorrey
Email: send email
Telefono: +34 918043347
Fax: +34 918043348

ES (TRES CANTOS MADRID) participant 315˙500.00
6    UNIVERSITA DEGLI STUDI MAGNA GRAECIA DI CATANZARO

 Organization address address: VIALE EUROPA LOCALITA GERMANETO
city: CATANZARO
postcode: 88100

contact info
Titolo: Mr.
Nome: Roberto
Cognome: Sigilli
Email: send email
Telefono: +39 09613694173
Fax: +39 09613695108

IT (CATANZARO) participant 271˙466.00
7    CONSIGLIO NAZIONALE DELLE RICERCHE

 Organization address address: Piazzale Aldo Moro 7
city: ROMA
postcode: 185

contact info
Titolo: Ms.
Nome: Martina
Cognome: Centazzo
Email: send email
Telefono: +39 040 3756411
Fax: +39 040 226767

IT (ROMA) participant 267˙448.00
8    FONDAZIONE ISTITUTO ITALIANO DI TECNOLOGIA

 Organization address address: VIA MOREGO 30
city: GENOVA
postcode: 16163

contact info
Titolo: Mr.
Nome: Gabriele
Cognome: Ballero
Email: send email
Telefono: +39 01071781445
Fax: +39 010720321

IT (GENOVA) participant 265˙889.60

Mappa


 Word cloud

Esplora la "nuvola delle parole (Word Cloud) per avere un'idea di massima del progetto.

performed    diagnosis    tasc    expertise    molecules    scientists    spectroscopy    device    electromagnetic    dynamic    infrared    detection    resolution    health    sub    membrane    fundamental    force    care    single    few    biological    proteins    optical    idea    molecule    thz    structural    smd    spatial    combine    diseases    plasmon    spectroscopic    nanotec    nm    protein    avenues    polaritons    detect    tweezer    receptors    cbm    chemical    metal    small    cancer    region    significant    characterisation    terahertz    raman    atomic   

 Obiettivo del progetto (Objective)

'Future breakthroughs in the understanding of fundamental biological processes causing major diseases are expected from the development of miniaturized probes or microscopes able to detect and identify a single or a small number of molecules. The SingleMoleculeDetection (SMD) proposal will develop a unique device able to perform simultaneously and in a dynamic way force and spectroscopic measurements. We will design and fabricate novel devices for the generation of plasmon polaritons as well as combine photonic crystals and plasmonic nanolenses. These new devices will be able to detect few/single molecules through Raman, InfraRed and Terahertz (THz) signals and in combination with Atomic Force Microscopy and Optical Tweezer force spectroscopy with a spatial resolution in the sub-10 nm for Raman and IR and sub-100 nm for the THz region. The complete characterization of single unknown molecule will be demonstrated through: i- investigations on the chemical and physical properties of membrane receptors, such as rhodopsin, odorant receptors and ionic channels; ii- identification of new molecules involved in cancer development and metastasis. The new devices will allow the acquisition of THz images and we will explore the possibilities of this new spectral region for biomedical scanning. The SMD proposal is based on an original idea of the coordinator, prof. E. di Fabrizio and will be exploited thanks to the complementary expertise present in the different sites and to a tight coordination between the various groups. The design, fabrication and testing will be performed at UMG, TASC and CBM Integration in a single instrument will be carried out at TASC, CBM, IIT Nanotec, RUB. Validation activities will be performed by all the partners taking advantage of the world leading expertise of the TUDO and the STRATH- AC in spectroscopy of natural and artificial biological systems. The SME NANOTEC and CBM will provide the commercial exploitation of the obtained results.'

Introduzione (Teaser)

A greater understanding of how individual molecules behave in biological systems will have significant implications for health care by opening up new avenues for intervention. A European consortium is contributing to this by developing new high-resolution devices that facilitate the detection of single molecules.

Descrizione progetto (Article)

The detection and characterisation of single molecules would facilitate research of fundamental biological processes and enable the recovery of structural information of membrane proteins. Moreover, diagnosis of many diseases including cancer could be improved.

To achieve this, scientists of the EU-funded 'Single or few molecules detection by combined enhanced spectroscopies' (SMD) project proposed to confine a high electromagnetic field in an extremely small portion of space to provide sensitive chemical mapping at the nano-scale level. To this end, they explored the properties of surface plasmon polaritons (SPPs), which are in essence electromagnetic waves created when light particles, also known as photons, impinge on metal plasmons under appropriate conditions.

The idea was to combine different experimental spectroscopy techniques in a single device so as to achieve label-free chemical characterisation at the single-molecule level. This system combines the technology of an atomic force microscope or an optical tweezer with optical spectroscopy, and allowed simultaneous dynamic way force and Raman, SERS, infrared or terahertz measurements. The spatial resolution achieved reached an unprecedented low level in the range of 10 nanometres or smaller.

Alongside the optimisation of the components, scientists synthesised an array of nanoprobes based on protein or DNA conjugates with metal ions. These were tested to obtain structural information and to characterise the conformational changes of the photoreceptor transmembrane protein in the cyclic nucleotide-gated ion channel. Additionally, by exploiting the BRCT domain proteins as probing molecules, researchers were able to distinguish the wild type from mutated peptides.

By successfully combining optical and mechanical (spectroscopic) characterisation methodologies in a single device, the SMD initiative addressed a significant technological challenge. Single-molecule spectroscopy has the potential to enhance diagnosis sensitivity and open up new avenues for pharmaceutical companies and industries interested in health care.

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