ATP dependent nucleosome remodelling - Single molecule studies and super-resolution microscopy


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 Nazionalità Coordinatore Germany [DE]
 Totale costo 1˙395˙381 €
 EC contributo 1˙395˙381 €
 Programma FP7-IDEAS-ERC
Specific programme: "Ideas" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013)
 Code Call ERC-2009-StG
 Funding Scheme ERC-SG
 Anno di inizio 2009
 Periodo (anno-mese-giorno) 2009-11-01   -   2014-10-31


# participant  country  role  EC contrib. [€] 

 Organization address address: GESCHWISTER SCHOLL PLATZ 1
postcode: 80539

contact info
Titolo: Mr.
Nome: Helmut
Cognome: Eckl
Email: send email
Telefono: +49 89 2180 3659
Fax: +49 89 2180 2985

DE (MUENCHEN) beneficiary 526˙319.00

 Organization address address: HELMHOLTZSTRASSE 16
city: ULM
postcode: 89081

contact info
Titolo: Mr.
Nome: Dieter
Cognome: Kaufmann
Email: send email
Telefono: +49 731 50 25000
Fax: +49 731 50 25007

DE (ULM) hostInstitution 869˙062.00

 Organization address address: HELMHOLTZSTRASSE 16
city: ULM
postcode: 89081

contact info
Titolo: Prof.
Nome: Jens
Cognome: Michaelis
Email: send email
Telefono: +49 731 50 23050
Fax: +49 731 50 23059

DE (ULM) hostInstitution 869˙062.00


 Word cloud

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atp    chromatin    mechanistic    dependent    microscopy    structures    nucleosomes    remodelling    fret    dna    nucleosome    determine    nucleosomal    fibers    nm   

 Obiettivo del progetto (Objective)

'In eukaryotic cells the DNA is packaged into nucleosomes and higher order structures which lead to a condensation and protection of the DNA. During important cellular processes such as transcription or replication access to the DNA has to be granted. This is facilitated by ATP dependent nucleosome remodelling. The mechanistic details how the involved remodelling complexes succeed in providing access to the nucleosomal DNA are currently in spite of large experimental efforts not well understood. The aim of the proposal is to unravel the molecular mechanism of nucleosome remodelling using single-molecule fluorescence resonance energy transfer (FRET). By putting labels on the nucleosomal DNA, the histones or the remodellers we will - step by step - determine the conformational changes that occur during remodelling and use this information to build a mechanistic model. We will also use the controlled assembly of 30 nm fibers from purified components in order to determine how remodelling occurs in this structurally restricted environment. The large size of the chromatin fibers will dictate that in addition to FRET measurements, which will again be used to investigate local motion, super-resolution microscopy need to be employed to obtain information about long-distance movements. To this end we will use stochastic optical reconstruction microscopy (STORM), which allows for accuracy below 10 nm, thus ideally complementing the FRET approach. In summary our experiments will lead us to a mechanistic understanding of ATP dependent nucleosome remodelling, both on mono-nucleosomes as well as in higher order structures. This knowledge will in return stimulate new initiatives aimed at understanding the nature and regulation of chromatin dynamics in vivo.'

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