HOTZYME

Systematic screening for novel hydrolases from hot environments

 Coordinatore KOBENHAVNS UNIVERSITET 

 Organization address postcode: 1017

contact info
Titolo: Mr.
Nome: Ivan
Cognome: Kristoffersen
Email: send email
Telefono: +45 35322626
Fax: +45 35324612

 Nazionalità Coordinatore Denmark [DK]
 Totale costo 7˙740˙992 €
 EC contributo 5˙518˙544 €
 Programma FP7-KBBE
Specific Programme "Cooperation": Food, Agriculture and Biotechnology
 Code Call FP7-KBBE-2010-4
 Funding Scheme CP-IP
 Anno di inizio 2011
 Periodo (anno-mese-giorno) 2011-04-01   -   2015-03-31

 Partecipanti

# participant  country  role  EC contrib. [€] 
1 KOBENHAVNS UNIVERSITET DK coordinator 1˙207˙873.00
2    THE UNIVERSITY OF EXETER

 Organization address address: Northcote House, The Queen's Drive
city: EXETER
postcode: EX4 4QJ

contact info
Titolo: Dr.
Nome: Clarke
Cognome: Enda
Email: send email
Telefono: +44 0 139 22 63744
Fax: +44 0 1392 263686

UK (EXETER) participant 465˙516.00
3    MICRODISH BV

 Organization address address: HAZENAKKER 18
city: HOUTEN
postcode: 3994 EJ

contact info
Titolo: Dr.
Nome: Martin
Cognome: Hessing
Email: send email
Telefono: +31 30 6343160
Fax: +31 30 6343161

NL (HOUTEN) participant 464˙160.00
4    EUROPEAN CENTRE FOR RESEARCH & FINANCING LTD

 Organization address address: HAMARVA STREET 15
city: BINYAMINA
postcode: 30500

contact info
Titolo: Prof.
Nome: Zohar
Cognome: Ben-Asher
Email: send email
Telefono: +972 3 6202255

IL (BINYAMINA) participant 452˙000.00
5    NATIONAL TECHNICAL UNIVERSITY OF ATHENS - NTUA

 Organization address address: HEROON POLYTECHNIOU 9 ZOGRAPHOU CAMPUS
city: ATHINA
postcode: 15780

contact info
Titolo: Ms.
Nome: Mertzelou
Cognome: Georgia
Email: send email
Telefono: 302108000000
Fax: 302108000000

EL (ATHINA) participant 403˙800.00
6    STIFTELSEN NORGES GEOTEKNISKEINSTITUTT

 Organization address address: Sognsveien 72
city: OSLO
postcode: N-0855

contact info
Titolo: Mr.
Nome: Robert
Cognome: Nakken
Email: send email
Telefono: +47 22023114
Fax: +47 22230448

NO (OSLO) participant 400˙943.00
7    NOVOZYMES A/S

 Organization address address: Krogshoejvej 36
city: BAGSVAERD
postcode: 2880

contact info
Titolo: Ms.
Nome: Nina
Cognome: Eckert Busgaard
Email: send email
Telefono: +45 44460794
Fax: +45 44469999

DK (BAGSVAERD) participant 396˙032.00
8    WAGENINGEN UNIVERSITY

 Organization address address: DROEVENDAALSESTEEG 4
city: WAGENINGEN
postcode: 6708 PB

contact info
Titolo: Mr.
Nome: Tom
Cognome: Bessems
Email: send email
Telefono: +31 317 484478
Fax: +31 317 481032

NL (WAGENINGEN) participant 366˙220.00
9    S.N. WINOGRADSKY INSTITUTE OF MICROBIOLOGY OF THE RUSSIAN ACADEMY OF SCIENCES

 Organization address address: PROSPEKT 60 LETIYA OKTYABRYA 7/2
city: MOSKVA
postcode: 117312

contact info
Titolo: Dr.
Nome: Elizaveta
Cognome: Bonch-Osmolovskaya
Email: send email
Telefono: 74991354458
Fax: +7 4991356530

RU (MOSKVA) participant 350˙000.00
10    Montana State University Bozeman

 Organization address address: Montana Hall 309
city: Bozeman
postcode: 59717

contact info
Titolo: Ms.
Nome: Susan
Cognome: Couch
Email: send email
Telefono: +001 1406 994 2080
Fax: +001 1406 994 5117

US (Bozeman) participant 304˙000.00
11    CONSIGLIO NAZIONALE DELLE RICERCHE

 Organization address address: Piazzale Aldo Moro 7
city: ROMA
postcode: 185

contact info
Titolo: Dr.
Nome: Sergio
Cognome: Riva
Email: send email
Telefono: 390229000000
Fax: 390229000000

IT (ROMA) participant 300˙000.00
12    SIGMA-ALDRICH PRODUCTION GMBH

 Organization address address: INDUSTRIESTRASSE 25
city: BUCHS
postcode: 9470

contact info
Titolo: Dr.
Nome: Roland
Cognome: Wohlgemuth
Email: send email
Telefono: +41 817552640
Fax: +41 817552640

CH (BUCHS) participant 210˙000.00
13    UNIVERSITAET DUISBURG-ESSEN

 Organization address address: UNIVERSITAETSSTRASSE 2
city: ESSEN
postcode: 45141

contact info
Titolo: Ms.
Nome: Sandra
Cognome: Kramm
Email: send email
Telefono: +49 203 3792661
Fax: +49 203 3791216

DE (ESSEN) participant 198˙000.00

Mappa


 Word cloud

Esplora la "nuvola delle parole (Word Cloud) per avere un'idea di massima del progetto.

degrading    promising    temperatures    energy    hydrolysis    search    enzymes    regarding    libraries    industrial    metagenomes    sequence    microbes    screening    scientists    reaction    bioethanol    addition    function    thermostable    functional    hydrolases    hot    hotzyme    benefits    environments    protein    environmental    degradation   

 Obiettivo del progetto (Objective)

'There is a strong need for new thermostable hydrolases with appropriate performance and/or novel functionalities that could provide huge savings in time, money and energy for industrial processes. The HotZyme project aims to identify such enzymes from hot terrestial environments, using metagenomic screening methods. New bioinfomatic tools will be developed to facilitate function prediction of genes from metagenomes that show low or no sequence homology to enzymes of known function. A range of high-throughput screening technologies will be employed to identify novel hydrolases. The consortium is composed of 13 partners from 10 European countries plus one partner from USA. The strong expertise in Microbiology, Moleculary Biology, Biochemistry, Biophysics, Geochemistry, Nanotechnology and Bioinformatics from our partners will be integrated in the project to ensure the fulfilment of the proposed tasks. Importantly, the five industrial partners, including three SMEs, will seek to commercialize the project results, thus ensuring a European wide impact, post project.'

Introduzione (Teaser)

New biotechnological advances are prompting the need to harness enzymes with novel functions for catalytic reactions. In particular, there is an increased demand for thermostable enzymes for use in bioethanol production, and pulp and starch processing.

Descrizione progetto (Article)

Catalysis plays an important role in many industrial processes, speeding the rate of a chemical reaction by lowering the energy required for the reaction to take place. Enzymes are a promising 'green' alternative to synthetic chemicals, yet very few are produced industrially. Finding or generating enzymes stable at the very high temperatures required of many industrial processes would have broad-reaching benefits. The search for heat-resistant enzymes provided the impetus for the EU-funded project 'Systematic screening for novel hydrolases from hot environments' (http://hotzyme.com/ (HOTZYME)).

Project scientists are using used state-of-the-art metagenomics screening methods. Predicting protein function as opposed to sequence screening allows functional screening of the collective genomes of microbes in their natural environments. The gene pool of interest comes from microbes living in hot terrestrial environments and the search is on for hydrolases. These enzymes catalyse hydrolysis, the splitting of a molecule through the addition of hydrogen and hydroxyl groups.

Researchers reported new findings regarding the promising enzymatic degradation activity of some isolates recovered from hot environments. These include isolation of a Thermus strain capable of degrading xylan at high temperatures and a thermoanaerobacter that shows resistance to epoxide. Two additional metagenomes were obtained from environmental sampling.

Other project achievements include the development of protein classifiers and a tracking system for samples, strains and libraries. Scientists developed different screening methods for rapid screening of lactonase and hydrolase enzymes. These enzymes were cloned and characterised for their substrate specificity and functional activity. In addition, eight expression libraries have been constructed and subjected to functional screening for novel hydrolases.

The expected final results include patenting of novel cellulases, xylases, xanthan gum degrading enzymes, and organisms with interesting polymer degradation characteristics.

HOTZYME findings should bridge the knowledge gap regarding the existence of more efficient enzymes at high temperatures than those produced by fungi to carry out cellulose hydrolysis. Important applications include bioethanol production from biomass conversion, offering significant environmental benefits compared to fossil fuels in terms of greenhouse gases.

Altri progetti dello stesso programma (FP7-KBBE)

EU-PLF (2012)

Bright Farm by Precision Livestock Farming

Read More  

GPLUSE (2014)

Genotype and Environment contributing to the sustainability of dairy cow production systems through the optimal integration of genomic selection and novel management protocols based on the development

Read More  

FACTOR MARKETS (2010)

Comparative Analysis of Factor Markets for Agriculture across the Member States

Read More