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Long-term molecular nanoscale imaging of neuronal function

Total Cost €


EC-Contrib. €






 MoNaLISA project word cloud

Explore the words cloud of the MoNaLISA project. It provides you a very rough idea of what is the project "MoNaLISA" about.

synaptic    photodamage    imaging    quantitative    1000    time    first    biogenesis    pioneered    difficult    close    counting    reaching    scales    milliseconds    powers    laser    combines    recording    gap    analyze    solution    30    line    tissues    termed    conventional    cells    poorer    recycling    acquisition    resolution    neuron    track    function    spaced    molecular    sted    patterns    2photon    paradigm    optics    neurons    slow    super    sensitive    microscope    expertise    nano    base    advantages    image    sciences    spatial    storm    fast    introduce    resolft    proof    impossible    life    microscopy    degradation    minimal    relying    potentially    sequential    proteins    combine    molecule    protein    small    temporal    insufficient    clusters    ssim    nanoscale    gsdim    3d    fill    monalisa    hours    obtain    fluorescence    days    living    live    palm    spanning    nanoscopy    record    vesicle    transmission    schemes    neuronal    organelles    faster    toxic    speed    switching    tightly    nm    detection    hz    ensemble    single   

Project "MoNaLISA" data sheet

The following table provides information about the project.


Organization address
postcode: 100 44

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Sweden [SE]
 Project website
 Total cost 1˙725˙000 €
 EC max contribution 1˙725˙000 € (100%)
 Programme 1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC))
 Code Call ERC-2014-STG
 Funding Scheme ERC-STG
 Starting year 2015
 Duration (year-month-day) from 2015-04-01   to  2020-03-31


Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 


 Project objective

Synaptic function is difficult to analyze in living neurons using conventional optics, since the synaptic organelles and protein clusters are small and tightly spaced. The solution to this problem can come from the field of super-resolution fluorescence microscopy, or nanoscopy. However, the current approaches to nanoscopy are still far from reaching this goal. Single molecule-based approaches (including STORM and PALM) provide high spatial resolution, but slow recording, insufficient for live imaging. Ensemble approaches (including SSIM and STED) are able to record faster, but with poorer resolution or with high, potentially toxic, laser powers. It is currently impossible to image the same neuron for hours and days, with both high spatial (~30 nm) and temporal (10-1000 Hz) resolution, and with minimal photodamage. My aim is to fill this gap, by developing, for the first time, a microscope that combines the advantages of both single molecule-based and ensemble approaches. I will base the microscope on RESOLFT, a low-photodamage ensemble approach that I have pioneered recently. I will use line patterns to speed up the recording and 2photon-switching for 3D ability. I will combine this with sensitive detection schemes that allow single-molecule detection and counting, relying on my previous expertise with PALM and GSDIM. The new set-up, termed molecular nanoscale long-term imaging with sequential acquisition (MoNaLISA), will track neuronal organelles and proteins on different time scales, spanning from milliseconds to days, with a resolution close to the molecular scale. To obtain the first proof-of-principle results, I will address several issues still open in the synaptic transmission field, relating to synaptic vesicle recycling, biogenesis and degradation. Overall, my project will introduce a novel paradigm to imaging in the life sciences, which will enable fast and quantitative nano-imaging of cells and tissues.


year authors and title journal last update
List of publications.
2018 Francesca Pennacchietti, Ekaterina O. Serebrovskaya, Aline R. Faro, Irina I. Shemyakina, Nina G. Bozhanova, Alexey A. Kotlobay, Nadya G. Gurskaya, Andreas Bodén, Jes Dreier, Dmitry M. Chudakov, Konstantin A. Lukyanov, Vladislav V. Verkhusha, Alexander S. Mishin, Ilaria Testa
Fast reversibly photoswitching red fluorescent proteins for live-cell RESOLFT nanoscopy
published pages: 601-604, ISSN: 1548-7091, DOI: 10.1038/s41592-018-0052-9
Nature Methods 15/8 2019-12-16

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