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Long-term molecular nanoscale imaging of neuronal function

Total Cost €


EC-Contrib. €






 MoNaLISA project word cloud

Explore the words cloud of the MoNaLISA project. It provides you a very rough idea of what is the project "MoNaLISA" about.

recording    microscopy    line    resolft    pioneered    potentially    fill    detection    scales    milliseconds    vesicle    ssim    powers    speed    slow    minimal    proof    image    patterns    tightly    impossible    temporal    schemes    molecule    difficult    single    first    insufficient    photodamage    termed    days    3d    combines    degradation    close    relying    nm    clusters    tissues    faster    ensemble    biogenesis    nanoscopy    small    hours    life    cells    spanning    spaced    proteins    track    sted    30    quantitative    analyze    expertise    toxic    super    neurons    paradigm    fast    sequential    gap    optics    hz    reaching    sensitive    protein    monalisa    conventional    synaptic    imaging    spatial    gsdim    sciences    living    2photon    nano    organelles    switching    recycling    nanoscale    molecular    live    1000    combine    poorer    transmission    palm    resolution    laser    record    base    time    solution    fluorescence    neuron    acquisition    counting    microscope    introduce    neuronal    function    obtain    storm    advantages   

Project "MoNaLISA" data sheet

The following table provides information about the project.


Organization address
postcode: 100 44

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Sweden [SE]
 Project website
 Total cost 1˙725˙000 €
 EC max contribution 1˙725˙000 € (100%)
 Programme 1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC))
 Code Call ERC-2014-STG
 Funding Scheme ERC-STG
 Starting year 2015
 Duration (year-month-day) from 2015-04-01   to  2020-03-31


Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 


 Project objective

Synaptic function is difficult to analyze in living neurons using conventional optics, since the synaptic organelles and protein clusters are small and tightly spaced. The solution to this problem can come from the field of super-resolution fluorescence microscopy, or nanoscopy. However, the current approaches to nanoscopy are still far from reaching this goal. Single molecule-based approaches (including STORM and PALM) provide high spatial resolution, but slow recording, insufficient for live imaging. Ensemble approaches (including SSIM and STED) are able to record faster, but with poorer resolution or with high, potentially toxic, laser powers. It is currently impossible to image the same neuron for hours and days, with both high spatial (~30 nm) and temporal (10-1000 Hz) resolution, and with minimal photodamage. My aim is to fill this gap, by developing, for the first time, a microscope that combines the advantages of both single molecule-based and ensemble approaches. I will base the microscope on RESOLFT, a low-photodamage ensemble approach that I have pioneered recently. I will use line patterns to speed up the recording and 2photon-switching for 3D ability. I will combine this with sensitive detection schemes that allow single-molecule detection and counting, relying on my previous expertise with PALM and GSDIM. The new set-up, termed molecular nanoscale long-term imaging with sequential acquisition (MoNaLISA), will track neuronal organelles and proteins on different time scales, spanning from milliseconds to days, with a resolution close to the molecular scale. To obtain the first proof-of-principle results, I will address several issues still open in the synaptic transmission field, relating to synaptic vesicle recycling, biogenesis and degradation. Overall, my project will introduce a novel paradigm to imaging in the life sciences, which will enable fast and quantitative nano-imaging of cells and tissues.


year authors and title journal last update
List of publications.
2018 Francesca Pennacchietti, Ekaterina O. Serebrovskaya, Aline R. Faro, Irina I. Shemyakina, Nina G. Bozhanova, Alexey A. Kotlobay, Nadya G. Gurskaya, Andreas Bodén, Jes Dreier, Dmitry M. Chudakov, Konstantin A. Lukyanov, Vladislav V. Verkhusha, Alexander S. Mishin, Ilaria Testa
Fast reversibly photoswitching red fluorescent proteins for live-cell RESOLFT nanoscopy
published pages: 601-604, ISSN: 1548-7091, DOI: 10.1038/s41592-018-0052-9
Nature Methods 15/8 2019-12-16

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