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Mechanobiology of nuclear import of transcription factors modeled within a bioengineered stem cell niche.

Total Cost €


EC-Contrib. €






 NICHOID project word cloud

Explore the words cloud of the NICHOID project. It provides you a very rough idea of what is the project "NICHOID" about.

microenvironment    nano    gene    computational    architectural    cues    successful    deeper    regulating    model    sensitive    photon    accurate    therapeutic    tissue    experimental    bioengineering    predictions    nuclear    fluorescently    niche    tridimensionality    ongoing    stretch    micro    mechano    breakthrough    combination    revolutionary    laser    labelled    mimicking    fabrication    repair    stem    niches    many    polymerization    mesenchymal    pore    idea    outcome    hypothesis    cultured    effect    biomaterial    substrate    innovative    fabricated    cells    differentially    combined    culture    adherent    ones    biomimetics    technologies    question    integrate    experimentally    direct    msc    import    multiscale    purpose    verify    synergic    spread    numerical    dimensional    substrates    geometry    transcription    mechanotransduction    regenerative    engineering    measured    biomimetic    synthetic    cell    complexes    effort    differentiation    native    fate    verified    medicine    multiphysics   

Project "NICHOID" data sheet

The following table provides information about the project.


Organization address
city: MILANO
postcode: 20133

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Italy [IT]
 Project website
 Total cost 1˙903˙330 €
 EC max contribution 1˙903˙330 € (100%)
 Programme 1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC))
 Code Call ERC-2014-CoG
 Funding Scheme ERC-COG
 Starting year 2015
 Duration (year-month-day) from 2015-05-01   to  2020-04-30


Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    POLITECNICO DI MILANO IT (MILANO) coordinator 1˙802˙830.00


 Project objective

Many therapeutic applications of stem cells require accurate control of their differentiation. To this purpose there is a major ongoing effort in the development of advanced culture substrates to be used as “synthetic niches” for the cells, mimicking the native ones. The goal of this project is to use a synthetic niche cell culture model to test my revolutionary hypothesis that in stem cell differentiation, nuclear import of gene-regulating transcription factors is controlled by the stretch of the nuclear pore complexes. If verified, this idea could lead to a breakthrough in biomimetic approaches to engineering stem cell differentiation. I investigate this question specifically in mesenchymal stem cells (MSC), because they are adherent and highly mechano-sensitive to architectural cues of the microenvironment. To verify my hypothesis I will use a combined experimental-computational model of mechanotransduction. I will a) scale-up an existing three-dimensional synthetic niche culture substrate, fabricated by two-photon laser polymerization, b) characterize the effect of tridimensionality on the differentiation fate of MSC cultured in the niches, c) develop a multiphysics/multiscale computational model of nuclear import of transcription factors within differentially-spread cultured cells, and d) integrate the numerical predictions with experimentally-measured import of fluorescently-labelled transcription factors. This project requires the synergic combination of several advanced bioengineering technologies, including micro/nano fabrication and biomimetics. The use of two-photon laser polymerization for controlling the geometry of the synthetic cell niches is very innovative and will highly impact the fields of bioengineering and biomaterial technology. A successful outcome will lead to a deeper understanding of bioengineering methods to direct stem cell fate and have therefore a significant impact in tissue repair technologies and regenerative medicine.


year authors and title journal last update
List of publications.
2018 Alberto García-González, Emanuela Jacchetti, Roberto Marotta, Marta Tunesi, José F. Rodríguez Matas, Manuela T. Raimondi
The Effect of Cell Morphology on the Permeability of the Nuclear Envelope to Diffusive Factors
published pages: , ISSN: 1664-042X, DOI: 10.3389/fphys.2018.00925
Frontiers in Physiology 9 2020-04-23
2019 Lucia Boeri, Diego Albani, Manuela Teresa Raimondi, Emanuela Jacchetti
Mechanical regulation of nucleocytoplasmic translocation in mesenchymal stem cells: characterization and methods for investigation
published pages: 817-831, ISSN: 1867-2450, DOI: 10.1007/s12551-019-00594-3
Biophysical Reviews 11/5 2020-04-23
2019 F. Donnaloja, E. Jacchetti, M. Soncini, M. T. Raimondi
Mechanosensing at the Nuclear Envelope by Nuclear Pore Complex Stretch Activation and Its Effect in Physiology and Pathology
published pages: , ISSN: 1664-042X, DOI: 10.3389/fphys.2019.00896
Frontiers in Physiology 10 2020-04-23
2016 Garcia Gonzales, A.; Marotta, R.; Tunesi, M.; Nava, M. M.; Fedele, R.; Jacchetti, E.; Rodriguez Matas, J. F.; Raimondi, M. T.
Integrated experimental/computational approach of the nuclear pore complex
published pages: , ISSN: 1473-2262, DOI:
European Cells and Materials 1 2020-04-23
2016 Michele M. Nava, Nunzia Di Maggio, Tommaso Zandrini, Giulio Cerullo, Roberto Osellame, Ivan Martin, Manuela T. Raimondi
Synthetic niche substrates engineered via two-photon laser polymerization for the expansion of human mesenchymal stromal cells
published pages: , ISSN: 1932-6254, DOI: 10.1002/term.2187
Journal of Tissue Engineering and Regenerative Medicine 2020-04-23
2018 Alessandro Marturano-Kruik, Michele Maria Nava, Keith Yeager, Alan Chramiec, Luke Hao, Samuel Robinson, Edward Guo, Manuela Teresa Raimondi, Gordana Vunjak-Novakovic
Human bone perivascular niche-on-a-chip for studying metastatic colonization
published pages: 1256-1261, ISSN: 0027-8424, DOI: 10.1073/pnas.1714282115
Proceedings of the National Academy of Sciences 115/6 2020-04-23
2016 Marta Tunesi, Federica Fusco, Fabio Fiordaliso, Alessandro Corbelli, Gloria Biella, Manuela T. Raimondi
Optimization of a 3D Dynamic Culturing System for In Vitro Modeling of Frontotemporal Neurodegeneration-Relevant Pathologic Features
published pages: , ISSN: 1663-4365, DOI: 10.3389/fnagi.2016.00146
Frontiers in Aging Neuroscience 8 2020-04-23
2017 Riccardo Sacco, Paola Causin, Chiara Lelli, Manuela T. Raimondi
A poroelastic mixture model of mechanobiological processes in biomass growth: theory and application to tissue engineering
published pages: 3273-3297, ISSN: 0025-6455, DOI: 10.1007/s11012-017-0638-9
Meccanica 52/14 2020-04-23
2017 Hadis Eghbali, Michele M. Nava, Gabriella Leonardi, Davod Mohebbi-Kalhori, Roberto Sebastiano, Abdolreza Samimi, Manuela T. Raimondi
An experimental-numerical investigation on the effects of macroporous scaffold geometry on cell culture parameters
published pages: 185-195, ISSN: 0391-3988, DOI: 10.5301/ijao.5000554
The International Journal of Artificial Organs 40/4 2020-04-23
2016 Hadis Eghbali, Michele M. Nava, Davod Mohebbi-Kalhori, Manuela T. Raimondi
Hollow fiber bioreactor technology for tissue engineering applications
published pages: 1-15, ISSN: 0391-3988, DOI: 10.5301/ijao.5000466
The International Journal of Artificial Organs 39/1 2020-04-23
2016 Michele M. Nava, Roberto Fedele, Manuela T. Raimondi
Computational prediction of strain-dependent diffusion of transcription factors through the cell nucleus
published pages: 983-993, ISSN: 1617-7959, DOI: 10.1007/s10237-015-0737-2
Biomechanics and Modeling in Mechanobiology 15/4 2020-04-23
2016 Laura Iannetti, Giovanna D’Urso, Gioacchino Conoscenti, Elena Cutrì, Rocky S. Tuan, Manuela T. Raimondi, Riccardo Gottardi, Paolo Zunino
Distributed and Lumped Parameter Models for the Characterization of High Throughput Bioreactors
published pages: e0162774, ISSN: 1932-6203, DOI: 10.1371/journal.pone.0162774
PLOS ONE 11/9 2020-04-23
2016 Alberto Garcia, Jose F. Rodriguez Matas, Manuela T. Raimondi
Modeling of the mechano-chemical behaviour of the nuclear pore complex: current research and perspectives
published pages: 1011-1021, ISSN: 1757-9694, DOI: 10.1039/C6IB00153J
Integr. Biol. 8/10 2020-04-23
2015 Manuela T. Raimondi, Carmen Giordano, Riccardo Pietrabissa
Oxygen measurement in interstitially perfused cellularized constructs cultured in a miniaturized bioreactor
published pages: 0-0, ISSN: 2280-8000, DOI: 10.5301/jabfm.5000246
Journal of Applied Biomaterials & Functional Materials 13/4 2020-04-23
2016 Michele M. Nava, Alessio Piuma, Marina Figliuzzi, Irene Cattaneo, Barbara Bonandrini, Tommaso Zandrini, Giulio Cerullo, Roberto Osellame, Andrea Remuzzi, Manuela T. Raimondi
Two-photon polymerized “nichoid” substrates maintain function of pluripotent stem cells when expanded under feeder-free conditions
published pages: , ISSN: 1757-6512, DOI: 10.1186/s13287-016-0387-z
Stem Cell Research & Therapy 7/1 2020-04-23
2017 Davide Ricci, Michele Nava, Tommaso Zandrini, Giulio Cerullo, Manuela Raimondi, Roberto Osellame
Scaling-Up Techniques for the Nanofabrication of Cell Culture Substrates via Two-Photon Polymerization for Industrial-Scale Expansion of Stem Cells
published pages: 66, ISSN: 1996-1944, DOI: 10.3390/ma10010066
Materials 10/1 2020-04-23

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