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NICHOID SIGNED

Mechanobiology of nuclear import of transcription factors modeled within a bioengineered stem cell niche.

Total Cost €

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EC-Contrib. €

0

Partnership

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 NICHOID project word cloud

Explore the words cloud of the NICHOID project. It provides you a very rough idea of what is the project "NICHOID" about.

niches    verified    geometry    synthetic    stem    substrate    mechanotransduction    pore    hypothesis    integrate    architectural    model    revolutionary    mechano    culture    cues    multiphysics    import    fabrication    micro    question    technologies    breakthrough    regenerative    medicine    experimentally    niche    tridimensionality    stretch    complexes    polymerization    msc    multiscale    outcome    successful    fate    ones    substrates    verify    repair    sensitive    mimicking    accurate    native    adherent    predictions    tissue    gene    idea    effort    differentially    deeper    laser    therapeutic    biomimetics    numerical    effect    spread    fluorescently    cells    differentiation    innovative    biomimetic    biomaterial    ongoing    transcription    microenvironment    fabricated    purpose    labelled    direct    engineering    cell    many    experimental    bioengineering    photon    nano    measured    synergic    cultured    mesenchymal    dimensional    regulating    combined    combination    computational    nuclear   

Project "NICHOID" data sheet

The following table provides information about the project.

Coordinator
POLITECNICO DI MILANO 

Organization address
address: PIAZZA LEONARDO DA VINCI 32
city: MILANO
postcode: 20133
website: www.polimi.it

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Italy [IT]
 Project website http://www.nichoid.polimi.it
 Total cost 1˙903˙330 €
 EC max contribution 1˙903˙330 € (100%)
 Programme 1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC))
 Code Call ERC-2014-CoG
 Funding Scheme ERC-COG
 Starting year 2015
 Duration (year-month-day) from 2015-05-01   to  2020-04-30

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    POLITECNICO DI MILANO IT (MILANO) coordinator 1˙802˙830.00
2    ISTITUTO DI RICERCHE FARMACOLOGICHE MARIO NEGRI IT (MILANO) participant 100˙500.00

Map

 Project objective

Many therapeutic applications of stem cells require accurate control of their differentiation. To this purpose there is a major ongoing effort in the development of advanced culture substrates to be used as “synthetic niches” for the cells, mimicking the native ones. The goal of this project is to use a synthetic niche cell culture model to test my revolutionary hypothesis that in stem cell differentiation, nuclear import of gene-regulating transcription factors is controlled by the stretch of the nuclear pore complexes. If verified, this idea could lead to a breakthrough in biomimetic approaches to engineering stem cell differentiation. I investigate this question specifically in mesenchymal stem cells (MSC), because they are adherent and highly mechano-sensitive to architectural cues of the microenvironment. To verify my hypothesis I will use a combined experimental-computational model of mechanotransduction. I will a) scale-up an existing three-dimensional synthetic niche culture substrate, fabricated by two-photon laser polymerization, b) characterize the effect of tridimensionality on the differentiation fate of MSC cultured in the niches, c) develop a multiphysics/multiscale computational model of nuclear import of transcription factors within differentially-spread cultured cells, and d) integrate the numerical predictions with experimentally-measured import of fluorescently-labelled transcription factors. This project requires the synergic combination of several advanced bioengineering technologies, including micro/nano fabrication and biomimetics. The use of two-photon laser polymerization for controlling the geometry of the synthetic cell niches is very innovative and will highly impact the fields of bioengineering and biomaterial technology. A successful outcome will lead to a deeper understanding of bioengineering methods to direct stem cell fate and have therefore a significant impact in tissue repair technologies and regenerative medicine.

 Publications

year authors and title journal last update
List of publications.
2018 Alberto García-González, Emanuela Jacchetti, Roberto Marotta, Marta Tunesi, José F. Rodríguez Matas, Manuela T. Raimondi
The Effect of Cell Morphology on the Permeability of the Nuclear Envelope to Diffusive Factors
published pages: , ISSN: 1664-042X, DOI: 10.3389/fphys.2018.00925
Frontiers in Physiology 9 2020-04-23
2019 Lucia Boeri, Diego Albani, Manuela Teresa Raimondi, Emanuela Jacchetti
Mechanical regulation of nucleocytoplasmic translocation in mesenchymal stem cells: characterization and methods for investigation
published pages: 817-831, ISSN: 1867-2450, DOI: 10.1007/s12551-019-00594-3
Biophysical Reviews 11/5 2020-04-23
2019 F. Donnaloja, E. Jacchetti, M. Soncini, M. T. Raimondi
Mechanosensing at the Nuclear Envelope by Nuclear Pore Complex Stretch Activation and Its Effect in Physiology and Pathology
published pages: , ISSN: 1664-042X, DOI: 10.3389/fphys.2019.00896
Frontiers in Physiology 10 2020-04-23
2016 Garcia Gonzales, A.; Marotta, R.; Tunesi, M.; Nava, M. M.; Fedele, R.; Jacchetti, E.; Rodriguez Matas, J. F.; Raimondi, M. T.
Integrated experimental/computational approach of the nuclear pore complex
published pages: , ISSN: 1473-2262, DOI:
European Cells and Materials 1 2020-04-23
2016 Michele M. Nava, Nunzia Di Maggio, Tommaso Zandrini, Giulio Cerullo, Roberto Osellame, Ivan Martin, Manuela T. Raimondi
Synthetic niche substrates engineered via two-photon laser polymerization for the expansion of human mesenchymal stromal cells
published pages: , ISSN: 1932-6254, DOI: 10.1002/term.2187
Journal of Tissue Engineering and Regenerative Medicine 2020-04-23
2018 Alessandro Marturano-Kruik, Michele Maria Nava, Keith Yeager, Alan Chramiec, Luke Hao, Samuel Robinson, Edward Guo, Manuela Teresa Raimondi, Gordana Vunjak-Novakovic
Human bone perivascular niche-on-a-chip for studying metastatic colonization
published pages: 1256-1261, ISSN: 0027-8424, DOI: 10.1073/pnas.1714282115
Proceedings of the National Academy of Sciences 115/6 2020-04-23
2016 Marta Tunesi, Federica Fusco, Fabio Fiordaliso, Alessandro Corbelli, Gloria Biella, Manuela T. Raimondi
Optimization of a 3D Dynamic Culturing System for In Vitro Modeling of Frontotemporal Neurodegeneration-Relevant Pathologic Features
published pages: , ISSN: 1663-4365, DOI: 10.3389/fnagi.2016.00146
Frontiers in Aging Neuroscience 8 2020-04-23
2017 Riccardo Sacco, Paola Causin, Chiara Lelli, Manuela T. Raimondi
A poroelastic mixture model of mechanobiological processes in biomass growth: theory and application to tissue engineering
published pages: 3273-3297, ISSN: 0025-6455, DOI: 10.1007/s11012-017-0638-9
Meccanica 52/14 2020-04-23
2017 Hadis Eghbali, Michele M. Nava, Gabriella Leonardi, Davod Mohebbi-Kalhori, Roberto Sebastiano, Abdolreza Samimi, Manuela T. Raimondi
An experimental-numerical investigation on the effects of macroporous scaffold geometry on cell culture parameters
published pages: 185-195, ISSN: 0391-3988, DOI: 10.5301/ijao.5000554
The International Journal of Artificial Organs 40/4 2020-04-23
2016 Hadis Eghbali, Michele M. Nava, Davod Mohebbi-Kalhori, Manuela T. Raimondi
Hollow fiber bioreactor technology for tissue engineering applications
published pages: 1-15, ISSN: 0391-3988, DOI: 10.5301/ijao.5000466
The International Journal of Artificial Organs 39/1 2020-04-23
2016 Michele M. Nava, Roberto Fedele, Manuela T. Raimondi
Computational prediction of strain-dependent diffusion of transcription factors through the cell nucleus
published pages: 983-993, ISSN: 1617-7959, DOI: 10.1007/s10237-015-0737-2
Biomechanics and Modeling in Mechanobiology 15/4 2020-04-23
2016 Laura Iannetti, Giovanna D’Urso, Gioacchino Conoscenti, Elena Cutrì, Rocky S. Tuan, Manuela T. Raimondi, Riccardo Gottardi, Paolo Zunino
Distributed and Lumped Parameter Models for the Characterization of High Throughput Bioreactors
published pages: e0162774, ISSN: 1932-6203, DOI: 10.1371/journal.pone.0162774
PLOS ONE 11/9 2020-04-23
2016 Alberto Garcia, Jose F. Rodriguez Matas, Manuela T. Raimondi
Modeling of the mechano-chemical behaviour of the nuclear pore complex: current research and perspectives
published pages: 1011-1021, ISSN: 1757-9694, DOI: 10.1039/C6IB00153J
Integr. Biol. 8/10 2020-04-23
2015 Manuela T. Raimondi, Carmen Giordano, Riccardo Pietrabissa
Oxygen measurement in interstitially perfused cellularized constructs cultured in a miniaturized bioreactor
published pages: 0-0, ISSN: 2280-8000, DOI: 10.5301/jabfm.5000246
Journal of Applied Biomaterials & Functional Materials 13/4 2020-04-23
2016 Michele M. Nava, Alessio Piuma, Marina Figliuzzi, Irene Cattaneo, Barbara Bonandrini, Tommaso Zandrini, Giulio Cerullo, Roberto Osellame, Andrea Remuzzi, Manuela T. Raimondi
Two-photon polymerized “nichoid” substrates maintain function of pluripotent stem cells when expanded under feeder-free conditions
published pages: , ISSN: 1757-6512, DOI: 10.1186/s13287-016-0387-z
Stem Cell Research & Therapy 7/1 2020-04-23
2017 Davide Ricci, Michele Nava, Tommaso Zandrini, Giulio Cerullo, Manuela Raimondi, Roberto Osellame
Scaling-Up Techniques for the Nanofabrication of Cell Culture Substrates via Two-Photon Polymerization for Industrial-Scale Expansion of Stem Cells
published pages: 66, ISSN: 1996-1944, DOI: 10.3390/ma10010066
Materials 10/1 2020-04-23

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