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TiFuN

Tiny Functional Au Nanorods: Novel NIR-Photothermal Nanoprobes for Single-Molecule Tracking at Confined Cellular Environment

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 Outcomes and results

 TiFuN project word cloud

Explore the words cloud of the TiFuN project. It provides you a very rough idea of what is the project "TiFuN" about.

molecule    tool    biology    world    elongated    sites    dgu    surface    imaging    limits    neurotransmitter    nanoparticles    spr    dynamics    photothermal    technique    expertise    core    lt    tackle    phi    setup    nanorods    domain    individual    organelles    regions    subsequently    multicolor    gold    synapse    harvested    smaller    nanoprobe    light    nm    specificity    laser    nir    proteins    cell    sensitivity    labeling    brilliant    signal    easily    optics    neuroscience    adhesion    window    tiny    protocol    volume    resonance    absorption    electron    receptors    size    nanoprobes    lounis    chemistry    property    probes    rods    fellow    centered    530    bioconjuagted    correlative    neural    exceptional    owing    photostability    detected    haystack    length    bioconjugated    tunable    dr    synapses    diameter    interdisciplinary    density    tifun    single    sources    integrin    needle    gradient    microscopy    nps    valuable    small    cellular    toxicity    plasmon    ultrahigh    np    group    display    spectral    combination    confined    choquet    prof    ratio    nanochemistry    background    centrifugation    utilize    detection   

Project "TiFuN" data sheet

The following table provides information about the project.

Coordinator		 CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE CNRS 

Organization address
address: RUE MICHEL ANGE 3
city: PARIS
postcode: 75794
website: www.cnrs.fr

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.
 Coordinator Country France [FR]
 Project website http://onlinelibrary.wiley.com/doi/10.1002/advs.201600280/epdf
 Total cost 185˙076 €
 EC max contribution 185˙076 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2014
 Funding Scheme MSCA-IF-EF-ST
 Starting year 2015
 Duration (year-month-day) from 2015-04-01   to  2017-03-31

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE CNRS FR (PARIS) coordinator 185˙076.00

Map

 Project objective

Photothermal Imaging (PhI) is an absorption detection technique used for the detection of single gold nanoparticles (NPs), which can be a valuable tool to tackle complex cellular activities. In order to study the dynamics of individual proteins in confined regions such as in neural synapses or in cell adhesion sites, small nanoprobes are needed. Gold NPs (~ 5 nm) can be used, however their surface plasmon resonance (SPR) is centered at 530 nm, a spectral domain where absorption of cellular organelles results in a background signal, which limits the sensitivity of PhI. A brilliant way to find the needle in the haystack is the development of a novel nanoprobe having NIR-absorption, smaller size and high specificity. In “TiFuN”, we will achieve this goal by developing tiny (length/diameter <10 nm/5 nm) gold nanorods (T-rods), an elongated gold NP with tunable SPR in the NIR-window. Different aspect ratio T-rods harvested using Density Gradient Ultrahigh (DGU) centrifugation protocol will be highly promising probes for multicolor labeling and detection of proteins. A dedicated PhI setup based on NIR-laser sources will also be built. Subsequently, the bioconjuagted T-rods and NIR-PhI will be used to study the dynamics of neurotransmitter receptors in synapse and integrin proteins in cell adhesion sites owing to their small core volume, NIR-absorption, exceptional photostability, and non-toxicity. Bioconjugated T-rods will also display the unique property of being easily detected with light and electron microscopy at the single- molecule level, and therefore will be a valuable tool for light/electron correlative microscopy. This highly interdisciplinary project (optics, chemistry and biology) will utilize the fellow’s expertise in nanochemistry in combination with world-leading expertise of Prof. Lounis group in single-molecule detection and Dr. Choquet institute in neuroscience and cell biology.

 Publications

year authors and title journal last update
List of publications.
2017 Edakkattuparambil Sidharth Shibu, Nadezda Varkentina, Laurent Cognet, Brahim Lounis
Small Gold Nanorods with Tunable Absorption for Photothermal Microscopy in Cells
published pages: 1600280, ISSN: 2198-3844, DOI: 10.1002/advs.201600280 		Advanced Science 4/2 2019-07-23

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