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TiFuN

Tiny Functional Au Nanorods: Novel NIR-Photothermal Nanoprobes for Single-Molecule Tracking at Confined Cellular Environment

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 Outcomes and results

 TiFuN project word cloud

Explore the words cloud of the TiFuN project. It provides you a very rough idea of what is the project "TiFuN" about.

dynamics    diameter    cellular    nanorods    phi    tunable    organelles    tifun    specificity    spectral    adhesion    surface    tackle    prof    detection    lt    photostability    valuable    imaging    size    biology    density    background    window    property    bioconjuagted    toxicity    subsequently    smaller    small    correlative    volume    sites    multicolor    nps    confined    optics    bioconjugated    electron    choquet    exceptional    easily    group    ratio    core    probes    tiny    interdisciplinary    tool    nanochemistry    nir    owing    chemistry    photothermal    labeling    brilliant    integrin    world    individual    rods    limits    centered    combination    fellow    nanoprobes    elongated    nm    molecule    neuroscience    gradient    signal    expertise    protocol    synapses    light    nanoparticles    proteins    dr    lounis    dgu    530    cell    display    domain    single    receptors    technique    setup    sources    sensitivity    ultrahigh    harvested    needle    microscopy    haystack    spr    laser    nanoprobe    np    centrifugation    regions    synapse    gold    utilize    neural    absorption    length    plasmon    detected    resonance    neurotransmitter   

Project "TiFuN" data sheet

The following table provides information about the project.

Coordinator		 CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE CNRS 

Organization address
address: RUE MICHEL ANGE 3
city: PARIS
postcode: 75794
website: www.cnrs.fr

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.
 Coordinator Country France [FR]
 Project website http://onlinelibrary.wiley.com/doi/10.1002/advs.201600280/epdf
 Total cost 185˙076 €
 EC max contribution 185˙076 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2014
 Funding Scheme MSCA-IF-EF-ST
 Starting year 2015
 Duration (year-month-day) from 2015-04-01   to  2017-03-31

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE CNRS FR (PARIS) coordinator 185˙076.00

Map

 Project objective

Photothermal Imaging (PhI) is an absorption detection technique used for the detection of single gold nanoparticles (NPs), which can be a valuable tool to tackle complex cellular activities. In order to study the dynamics of individual proteins in confined regions such as in neural synapses or in cell adhesion sites, small nanoprobes are needed. Gold NPs (~ 5 nm) can be used, however their surface plasmon resonance (SPR) is centered at 530 nm, a spectral domain where absorption of cellular organelles results in a background signal, which limits the sensitivity of PhI. A brilliant way to find the needle in the haystack is the development of a novel nanoprobe having NIR-absorption, smaller size and high specificity. In “TiFuN”, we will achieve this goal by developing tiny (length/diameter <10 nm/5 nm) gold nanorods (T-rods), an elongated gold NP with tunable SPR in the NIR-window. Different aspect ratio T-rods harvested using Density Gradient Ultrahigh (DGU) centrifugation protocol will be highly promising probes for multicolor labeling and detection of proteins. A dedicated PhI setup based on NIR-laser sources will also be built. Subsequently, the bioconjuagted T-rods and NIR-PhI will be used to study the dynamics of neurotransmitter receptors in synapse and integrin proteins in cell adhesion sites owing to their small core volume, NIR-absorption, exceptional photostability, and non-toxicity. Bioconjugated T-rods will also display the unique property of being easily detected with light and electron microscopy at the single- molecule level, and therefore will be a valuable tool for light/electron correlative microscopy. This highly interdisciplinary project (optics, chemistry and biology) will utilize the fellow’s expertise in nanochemistry in combination with world-leading expertise of Prof. Lounis group in single-molecule detection and Dr. Choquet institute in neuroscience and cell biology.

 Publications

year authors and title journal last update
List of publications.
2017 Edakkattuparambil Sidharth Shibu, Nadezda Varkentina, Laurent Cognet, Brahim Lounis
Small Gold Nanorods with Tunable Absorption for Photothermal Microscopy in Cells
published pages: 1600280, ISSN: 2198-3844, DOI: 10.1002/advs.201600280 		Advanced Science 4/2 2019-07-23

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