Opendata, web and dolomites

TiFuN

Tiny Functional Au Nanorods: Novel NIR-Photothermal Nanoprobes for Single-Molecule Tracking at Confined Cellular Environment

Total Cost €

0

EC-Contrib. €

0

Partnership

0

Views

0

 TiFuN project word cloud

Explore the words cloud of the TiFuN project. It provides you a very rough idea of what is the project "TiFuN" about.

optics    single    tool    specificity    neural    haystack    utilize    nanoparticles    neurotransmitter    phi    spr    display    brilliant    proteins    sources    lt    dr    synapse    microscopy    absorption    ultrahigh    laser    needle    nanochemistry    integrin    ratio    tiny    subsequently    np    labeling    plasmon    world    interdisciplinary    volume    cellular    expertise    background    toxicity    dynamics    easily    electron    detected    centered    resonance    exceptional    530    receptors    domain    rods    nanoprobe    nanoprobes    prof    nir    bioconjugated    dgu    organelles    signal    group    size    small    protocol    tifun    detection    lounis    chemistry    cell    adhesion    technique    photothermal    length    bioconjuagted    gradient    property    gold    core    sensitivity    surface    photostability    light    confined    harvested    sites    smaller    nm    nanorods    limits    density    individual    tackle    choquet    elongated    centrifugation    nps    valuable    multicolor    tunable    probes    neuroscience    biology    imaging    owing    fellow    correlative    window    spectral    diameter    setup    combination    regions    synapses    molecule   

Project "TiFuN" data sheet

The following table provides information about the project.

Coordinator
CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE CNRS 

Organization address
address: RUE MICHEL ANGE 3
city: PARIS
postcode: 75794
website: www.cnrs.fr

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country France [FR]
 Project website http://onlinelibrary.wiley.com/doi/10.1002/advs.201600280/epdf
 Total cost 185˙076 €
 EC max contribution 185˙076 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2014
 Funding Scheme MSCA-IF-EF-ST
 Starting year 2015
 Duration (year-month-day) from 2015-04-01   to  2017-03-31

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE CNRS FR (PARIS) coordinator 185˙076.00

Map

 Project objective

Photothermal Imaging (PhI) is an absorption detection technique used for the detection of single gold nanoparticles (NPs), which can be a valuable tool to tackle complex cellular activities. In order to study the dynamics of individual proteins in confined regions such as in neural synapses or in cell adhesion sites, small nanoprobes are needed. Gold NPs (~ 5 nm) can be used, however their surface plasmon resonance (SPR) is centered at 530 nm, a spectral domain where absorption of cellular organelles results in a background signal, which limits the sensitivity of PhI. A brilliant way to find the needle in the haystack is the development of a novel nanoprobe having NIR-absorption, smaller size and high specificity. In “TiFuN”, we will achieve this goal by developing tiny (length/diameter <10 nm/5 nm) gold nanorods (T-rods), an elongated gold NP with tunable SPR in the NIR-window. Different aspect ratio T-rods harvested using Density Gradient Ultrahigh (DGU) centrifugation protocol will be highly promising probes for multicolor labeling and detection of proteins. A dedicated PhI setup based on NIR-laser sources will also be built. Subsequently, the bioconjuagted T-rods and NIR-PhI will be used to study the dynamics of neurotransmitter receptors in synapse and integrin proteins in cell adhesion sites owing to their small core volume, NIR-absorption, exceptional photostability, and non-toxicity. Bioconjugated T-rods will also display the unique property of being easily detected with light and electron microscopy at the single- molecule level, and therefore will be a valuable tool for light/electron correlative microscopy. This highly interdisciplinary project (optics, chemistry and biology) will utilize the fellow’s expertise in nanochemistry in combination with world-leading expertise of Prof. Lounis group in single-molecule detection and Dr. Choquet institute in neuroscience and cell biology.

 Publications

year authors and title journal last update
List of publications.
2017 Edakkattuparambil Sidharth Shibu, Nadezda Varkentina, Laurent Cognet, Brahim Lounis
Small Gold Nanorods with Tunable Absorption for Photothermal Microscopy in Cells
published pages: 1600280, ISSN: 2198-3844, DOI: 10.1002/advs.201600280
Advanced Science 4/2 2019-07-23

Are you the coordinator (or a participant) of this project? Plaese send me more information about the "TIFUN" project.

For instance: the website url (it has not provided by EU-opendata yet), the logo, a more detailed description of the project (in plain text as a rtf file or a word file), some pictures (as picture files, not embedded into any word file), twitter account, linkedin page, etc.

Send me an  email (fabio@fabiodisconzi.com) and I put them in your project's page as son as possible.

Thanks. And then put a link of this page into your project's website.

The information about "TIFUN" are provided by the European Opendata Portal: CORDIS opendata.

More projects from the same programme (H2020-EU.1.3.2.)

EVOMET (2019)

The rise and fall of metastatic clones under immune attack

Read More  

SpaTime_AnTB (2020)

Single-cell spatiotemporal analysis of Mycobacterium tuberculosis responses to antibiotics within host microenvironments

Read More  

TCFLAND2SEA (2020)

Thawing Carbon From LAND to SEA: Microbial Degradation of Organic Matter and Response to Thawing Permafrost in the Northeast Siberian Land-Shelf System

Read More