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TiFuN

Tiny Functional Au Nanorods: Novel NIR-Photothermal Nanoprobes for Single-Molecule Tracking at Confined Cellular Environment

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 Outcomes and results

 TiFuN project word cloud

Explore the words cloud of the TiFuN project. It provides you a very rough idea of what is the project "TiFuN" about.

cellular    bioconjugated    tool    centrifugation    regions    phi    integrin    proteins    background    choquet    protocol    signal    organelles    plasmon    tiny    spectral    ratio    bioconjuagted    display    exceptional    cell    lounis    sensitivity    owing    detected    technique    gold    nanoparticles    dr    single    sites    multicolor    labeling    window    dynamics    dgu    smaller    size    tifun    toxicity    detection    haystack    domain    absorption    probes    light    density    electron    nanochemistry    surface    valuable    nps    needle    length    nm    receptors    photostability    group    small    interdisciplinary    chemistry    specificity    530    synapse    subsequently    synapses    imaging    rods    volume    neuroscience    biology    centered    neurotransmitter    microscopy    np    core    tunable    confined    limits    setup    resonance    world    harvested    optics    fellow    expertise    utilize    nir    diameter    ultrahigh    property    individual    elongated    prof    molecule    brilliant    laser    lt    correlative    sources    nanoprobe    adhesion    easily    combination    spr    tackle    photothermal    nanorods    nanoprobes    gradient    neural   

Project "TiFuN" data sheet

The following table provides information about the project.

Coordinator		 CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE CNRS 

Organization address
address: RUE MICHEL ANGE 3
city: PARIS
postcode: 75794
website: www.cnrs.fr

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.
 Coordinator Country France [FR]
 Project website http://onlinelibrary.wiley.com/doi/10.1002/advs.201600280/epdf
 Total cost 185˙076 €
 EC max contribution 185˙076 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2014
 Funding Scheme MSCA-IF-EF-ST
 Starting year 2015
 Duration (year-month-day) from 2015-04-01   to  2017-03-31

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE CNRS FR (PARIS) coordinator 185˙076.00

Map

 Project objective

Photothermal Imaging (PhI) is an absorption detection technique used for the detection of single gold nanoparticles (NPs), which can be a valuable tool to tackle complex cellular activities. In order to study the dynamics of individual proteins in confined regions such as in neural synapses or in cell adhesion sites, small nanoprobes are needed. Gold NPs (~ 5 nm) can be used, however their surface plasmon resonance (SPR) is centered at 530 nm, a spectral domain where absorption of cellular organelles results in a background signal, which limits the sensitivity of PhI. A brilliant way to find the needle in the haystack is the development of a novel nanoprobe having NIR-absorption, smaller size and high specificity. In “TiFuN”, we will achieve this goal by developing tiny (length/diameter <10 nm/5 nm) gold nanorods (T-rods), an elongated gold NP with tunable SPR in the NIR-window. Different aspect ratio T-rods harvested using Density Gradient Ultrahigh (DGU) centrifugation protocol will be highly promising probes for multicolor labeling and detection of proteins. A dedicated PhI setup based on NIR-laser sources will also be built. Subsequently, the bioconjuagted T-rods and NIR-PhI will be used to study the dynamics of neurotransmitter receptors in synapse and integrin proteins in cell adhesion sites owing to their small core volume, NIR-absorption, exceptional photostability, and non-toxicity. Bioconjugated T-rods will also display the unique property of being easily detected with light and electron microscopy at the single- molecule level, and therefore will be a valuable tool for light/electron correlative microscopy. This highly interdisciplinary project (optics, chemistry and biology) will utilize the fellow’s expertise in nanochemistry in combination with world-leading expertise of Prof. Lounis group in single-molecule detection and Dr. Choquet institute in neuroscience and cell biology.

 Publications

year authors and title journal last update
List of publications.
2017 Edakkattuparambil Sidharth Shibu, Nadezda Varkentina, Laurent Cognet, Brahim Lounis
Small Gold Nanorods with Tunable Absorption for Photothermal Microscopy in Cells
published pages: 1600280, ISSN: 2198-3844, DOI: 10.1002/advs.201600280 		Advanced Science 4/2 2019-07-23

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