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ReguloBac-3UTR SIGNED

High-throughput in vivo studies on posttranscriptional regulatory mechanisms mediated by bacterial 3'-UTRs

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EC-Contrib. €

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Partnership

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 ReguloBac-3UTR project word cloud

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Project "ReguloBac-3UTR" data sheet

The following table provides information about the project.

Coordinator
AGENCIA ESTATAL CONSEJO SUPERIOR DEINVESTIGACIONES CIENTIFICAS 

Organization address
address: CALLE SERRANO 117
city: MADRID
postcode: 28006
website: http://www.csic.es

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Spain [ES]
 Project website http://idab.es/grupo-de-investigacion-de-regulacion-genica-bacteriana/erc-cog-regulobac-3-utrs/
 Total cost 1˙876˙778 €
 EC max contribution 1˙876˙778 € (100%)
 Programme 1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC))
 Code Call ERC-2014-CoG
 Funding Scheme ERC-COG
 Starting year 2015
 Duration (year-month-day) from 2015-09-01   to  2020-08-31

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    AGENCIA ESTATAL CONSEJO SUPERIOR DEINVESTIGACIONES CIENTIFICAS ES (MADRID) coordinator 1˙876˙778.00

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 Project objective

In eukaryotes, untranslated regions located at the 3′ end (3’UTRs) of messenger RNAs (mRNAs) have been proved to be key post-transcriptional regulatory elements controlling almost every single biological process. In contrast, in bacteria, most studies regarding post-transcriptional regulation have been mainly focused on specific non-coding RNAs and 5’UTRs, which often carry riboswitches or thermosensors. Remarkably, bacterial 3’UTRs have been largely disregarded and have not been considered as potential regulators. Recently, we found that a 3’UTR modulates biofilm formation in S. aureus through its interaction with the 5’UTR encoded in the same mRNA. This mechanism resembles eukaryotic mRNA circularization. Also, a 3’UTR that contributes to cellular homeostasis by promoting hilD mRNA turnover was recently shown in Salmonella. Although both studies are pioneering showing the potential of bacterial 3’UTRs as regulatory elements, many questions still remain to be answered. Are 3’UTRs roles conserved in bacterial species? Do 3’UTRs contain specific regulatory sequences or secondary RNA structures? Are transcriptional terminator sequences relevant for certain 3’UTRs? Are 3’UTRs specifically recognized by RNA-binding proteins? Might 3’UTRs be responsible for bacterial speciation? Might bacterial 3’UTRs be the ancestors of eukaryotic 3’UTR evolution? To achieve these questions, here we propose a high-throughput analysis based on the development of specialized dual-reporter libraries to identify in vivo functional 3’UTRs by fluorescence-activated cell sorting coupled to RNA sequencing. Also the pool of RNA-binding proteins associated to 3’UTRs will be identified by global MS2-tagging and mass spectrometry. Examples of 3’UTRs belonging to physiologically important genes will be selected to deeply study regulatory mechanisms at the molecular and single cell levels. We expect that this project will largely change the view of post-transcriptional regulation in bacteria.

 Publications

year authors and title journal last update
List of publications.
2020 Arancha Catalan‐Moreno, Carlos J. Caballero, Naiara Irurzun, Sergio Cuesta, Jacinto López‐Sagaseta, Alejandro Toledo‐Arana
One evolutionarily selected amino acid variation is sufficient to provide functional specificity in the cold shock protein paralogs of Staphylococcus aureus
published pages: , ISSN: 0950-382X, DOI: 10.1111/mmi.14446
Molecular Microbiology 2020-04-15
2020 Alejandro Toledo‐Arana, Iñigo Lasa
Advances in bacterial transcriptome understanding: From overlapping transcription to the excludon concept
published pages: 593-602, ISSN: 0950-382X, DOI: 10.1111/mmi.14456
Molecular Microbiology 113/3 2020-04-15
2020 Pilar Menendez-Gil, Carlos J Caballero, Arancha Catalan-Moreno, Naiara Irurzun, Inigo Barrio-Hernandez, Isabelle Caldelari, Alejandro Toledo-Arana
Differential evolution in 3′UTRs leads to specific gene expression in Staphylococcus
published pages: 2544-2563, ISSN: 0305-1048, DOI: 10.1093/nar/gkaa047
Nucleic Acids Research 48/5 2020-04-15
2020 Arancha Catalan‐Moreno, Carlos J. Caballero, Naiara Irurzun, Sergio Cuesta, Jacinto López‐Sagaseta, Alejandro Toledo‐Arana
One evolutionarily selected amino acid variation is sufficient to provide functional specificity in the cold shock protein paralogs of Staphylococcus aureus
published pages: , ISSN: 0950-382X, DOI: 10.1111/mmi.14446
Molecular Microbiology 2020-02-13
2019 Delphine Bronesky, Emma Desgranges, Anna Corvaglia, Patrice François, Carlos J Caballero, Laura Prado, Alejandro Toledo‐Arana, Inigo Lasa, Karen Moreau, François Vandenesch, Stefano Marzi, Pascale Romby, Isabelle Caldelari
A multifaceted small RNA modulates gene expression upon glucose limitation in Staphylococcus aureus
published pages: , ISSN: 0261-4189, DOI: 10.15252/embj.201899363
The EMBO Journal 38/6 2020-02-13
2019 S. Sáenz-Lahoya, N. Bitarte, B. García, S. Burgui, M. Vergara-Irigaray, J. Valle, C. Solano, A. Toledo-Arana, I. Lasa
Noncontiguous operon is a genetic organization for coordinating bacterial gene expression
published pages: 1733-1738, ISSN: 0027-8424, DOI: 10.1073/pnas.1812746116
Proceedings of the National Academy of Sciences 116/5 2020-02-13
2017 L. Bastet, C. Caballero, M. Villanueva, I. Lasa y A. Toledo-Arana
An antisense RNA connects SOS-response to cellular stress
published pages: , ISSN: , DOI:
FEMS 2017: 7th Congress of European Microbiologists 1 2019-05-31
2016 L. Bastet, C. Caballero, M. Villanueva, I. Lasa, A. Toledo-Arana
Un tránscrito antisentido conecta el estrés celular con la respuesta SOS
published pages: , ISSN: , DOI:
XI Molecular Microbiology Meeting of the Spanish Society of Microbiology 1 2019-05-31
2016 P. Menendez-Gil, C. Caballero, A. Toledo-Arana
Evolución diferencial de las 3\'UTRs en bacterias
published pages: , ISSN: , DOI:
XI Molecular Microbiology Meeting of the Spanish Society of Microbiology 1 2019-05-31
2017 A. Catalán, C. Caballero and A. Toledo-Arana
Identification and characterization of RNA-binding proteins in Staphylococcus aureus
published pages: , ISSN: , DOI:
FEMS 2017: 7th Congress of European Microbiologists 1 2019-05-31
2016 C. Caballero, A. Catalán Moreno, M. Vergara-Irigaray, B. García, C. Solano Goñi, I. Lasa, A. Toledo-Arana
Caracterización funcional de las chaperonas de RNA en Staphylococcus aureus
published pages: , ISSN: , DOI:
XI Molecular Microbiology Meeting of the Spanish Society of Microbiology 1 2019-05-31
2017 P. Menéndez-Gil, C. Caballero and A. Toledo-Arana
3’UTRs: new post-transcriptional regulatory elements in bacteria
published pages: , ISSN: , DOI:
FEMS 2017: 7th Congress of European Microbiologists 1 2019-05-31
2018 Carlos J Caballero, Pilar Menendez-Gil, Arancha Catalan-Moreno, Marta Vergara-Irigaray, Begoña García, Víctor Segura, Naiara Irurzun, Maite Villanueva, Igor Ruiz de los Mozos, Cristina Solano, Iñigo Lasa, Alejandro Toledo-Arana
The regulon of the RNA chaperone CspA and its auto-regulation in Staphylococcus aureus
published pages: 1345-1361, ISSN: 0305-1048, DOI: 10.1093/nar/gkx1284
Nucleic Acids Research 46/3 2019-05-31

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