Opendata, web and dolomites

ReachingCompleteness SIGNED

The Molecular Basis of Somatic Nuclear Reprogramming

Total Cost €

0

EC-Contrib. €

0

Partnership

0

Views

0

 ReachingCompleteness project word cloud

Explore the words cloud of the ReachingCompleteness project. It provides you a very rough idea of what is the project "ReachingCompleteness" about.

transcriptional    quality    mechanisms    ideal    transcriptome    detect    fish    infancy    crispr    resource    prevailing    monitor    intend    technologies    cells    molecular    invaluable    generation    rare    pluripotent    capture    knock    reporter    ipscs    edge    noise    somatic    cell    rna    employing    grant    uncover    poor    mrna    tests    cutting    single    segregate    degree    overcome    progress    profile    ratio    tools    solely    stable    measured    trace    stringent    reprogramming    disease    bioinformatic    patient    molecule    parallel    types    stem    nuclear    aberrant    therapy    cas9    sophisticated    complete    developmental    hinder    suggests    hurdles    screening    safe    pluripotency    drug    incomplete    leads    conversion    signal    basic    seq    exhibit    majority    fluidigm    limitations    successful    models    overview    efforts    direct    reprogrammable    fluorescent    converted    critical    dictate    global    deciphering    biomark    uncovering    vast    establishing   

Project "ReachingCompleteness" data sheet

The following table provides information about the project.

Coordinator
THE HEBREW UNIVERSITY OF JERUSALEM 

Organization address
address: EDMOND J SAFRA CAMPUS GIVAT RAM
city: JERUSALEM
postcode: 91904
website: www.huji.ac.il

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Israel [IL]
 Project website http://www.buganimlab.com
 Total cost 1˙500˙000 €
 EC max contribution 1˙500˙000 € (100%)
 Programme 1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC))
 Code Call ERC-2015-STG
 Funding Scheme ERC-STG
 Starting year 2016
 Duration (year-month-day) from 2016-03-01   to  2021-02-28

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    THE HEBREW UNIVERSITY OF JERUSALEM IL (JERUSALEM) coordinator 1˙500˙000.00

Map

 Project objective

The direct conversion approach and the generation of induced pluripotent stem cells (iPSCs) provide an invaluable resource of cells for disease modelling, drug screening, and patient-specific cell-based therapy. However, the directly converted cells are not stable, and the vast majority of iPSCs exhibit poor developmental potential as measured by stringent pluripotency tests. This suggests that the prevailing method of reprogramming is not ideal and leads to aberrant/incomplete conversion. To improve the quality of the converted cells, efforts should be focused on uncovering the molecular mechanisms that characterize the nuclear reprogramming process. There are two critical hurdles that hinder the progress of deciphering the elements that dictate successful reprogramming: (1) The ability to detect and capture solely the rare cells that eventually will be converted and (2) to monitor the transcriptional profile of cells at the single-cell level. Single-cell technology is in its infancy and many of the methods used today are characterized by high noise to signal ratio. In this grant proposal we intend to overcome these limitations by (1) establishing a complex fluorescent knock-in reporter system using the CRISPR/Cas9 method to capture the early rare reprogrammable cells and by (2) employing several cutting-edge single-cell technologies, RNA-Seq, Fluidigm BioMark and single-molecule mRNA-FISH, to segregate the real signal from the noise. To identify common and more global elements that facilitate nuclear reprogramming at large, we will trace in parallel, reprogrammable cells from two different somatic cell conversion models that reach high degree of nuclear reprogramming, and analyse their transcriptome using sophisticated bioinformatic tools. This study will provide a general overview of the changes that occur during the conversion of various cell types and will uncover the basic features that are essential to reach safe and complete conversion.

 Publications

year authors and title journal last update
List of publications.
2017 Mohammad Jaber, Shulamit Sebban, Yosef Buganim
Acquisition of the pluripotent and trophectoderm states in the embryo and during somatic nuclear reprogramming
published pages: 37-43, ISSN: 0959-437X, DOI: 10.1016/j.gde.2017.06.012
Current Opinion in Genetics & Development 46 2019-07-08

Are you the coordinator (or a participant) of this project? Plaese send me more information about the "REACHINGCOMPLETENESS" project.

For instance: the website url (it has not provided by EU-opendata yet), the logo, a more detailed description of the project (in plain text as a rtf file or a word file), some pictures (as picture files, not embedded into any word file), twitter account, linkedin page, etc.

Send me an  email (fabio@fabiodisconzi.com) and I put them in your project's page as son as possible.

Thanks. And then put a link of this page into your project's website.

The information about "REACHINGCOMPLETENESS" are provided by the European Opendata Portal: CORDIS opendata.

More projects from the same programme (H2020-EU.1.1.)

Aware (2019)

Aiding Antibiotic Development with Deep Analysis of Resistance Evolution

Read More  

Resonances (2019)

Resonances and Zeta Functions in Smooth Ergodic Theory and Geometry

Read More  

CURVE-X (2019)

Industrialisation of curved sensors and related imagers

Read More