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SINCHAIS SIGNED

In situ analysis of single channel subunit composition in neurons: physiological implication in synaptic plasticity and behavior

Total Cost €

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EC-Contrib. €

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Partnership

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 Outcomes and results

 SINCHAIS project word cloud

Explore the words cloud of the SINCHAIS project. It provides you a very rough idea of what is the project "SINCHAIS" about.

individual    postsynaptic    property    inward    detection    location    manipulation    resolution    labelling    combining    composition    elicits    efficacy    plasticity    effectors    respectively    molecules    subunit    diversity    examine    time    ones    paradigms    synapses    course    coupling    chemical    biochemical    compositions    technologies    unprecedented    plastic    intact    single    little    visualize    currents    lack    tissues    glutamate    brain    transforming    optogenetic    insights    electron    physiological    alteration    freeze    contributes    release    situ    views    combined    electrical    triggers    replica    gated    microscopy    electrophysiological    animal    regulation    channel    channels    potentiation    protein    em    ligand    dynamic    subunits    sites    signals    voltage    receptors    neurons    interaction    synaptic    presynaptic    trafficking    ampa    behavioural    activation    functions    calcium    depression    connections    vesicle    mice    tags    stimulation    implications    genetic    tomography    excitatory    fracture    nmda   

Project "SINCHAIS" data sheet

The following table provides information about the project.

Coordinator		 INSTITUTE OF SCIENCE AND TECHNOLOGY AUSTRIA 

Organization address
address: Am Campus 1
city: KLOSTERNEUBURG
postcode: 3400
website: www.ist.ac.at

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.
 Coordinator Country Austria [AT]
 Project website https://ist.ac.at/research/research-groups/shigemoto-group/erc-advanced-grant/
 Total cost 2˙481˙437 €
 EC max contribution 2˙481˙437 € (100%)
 Programme 1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC))
 Code Call ERC-2015-AdG
 Funding Scheme ERC-ADG
 Starting year 2016
 Duration (year-month-day) from 2016-07-01   to  2021-06-30

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    INSTITUTE OF SCIENCE AND TECHNOLOGY AUSTRIA AT (KLOSTERNEUBURG) coordinator 2˙481˙437.00

Map

 Project objective

Ligand-gated and voltage-gated channels are key molecules in transforming chemical signals into electrical ones and electrical signals into chemical ones, respectively. At excitatory synaptic connections in the brain, activation of AMPA- and NMDA-type glutamate receptors elicits inward currents at the postsynaptic sites, and activation of voltage-gated calcium channels triggers vesicle release of glutamate in the presynaptic sites. Plastic changes in their number, location and property can lead to potentiation or depression of synaptic efficacy, alteration in time course, and coupling to effectors at both postsynaptic and presynaptic sites. These channels are all composed of distinct subunits and their compositions affect channel properties, trafficking to the synaptic sites, and interaction with associated molecules, creating a large diversity of synaptic functions. Although channels with different subunit compositions have been investigated using biochemical and electrophysiological detection methods, very little is known about single channel subunit composition in situ because of the lack of high resolution methods for analysis of protein complex in intact tissues. In this project, I will develop novel technologies to visualize subunit composition at the single channel level in individual synapses by electron microscopy, combining new EM tags, freeze-fracture replica labelling, and electron tomography. Synaptic plasticity will be induced by optogenetic stimulation of identified neurons or behavioural paradigms to examine the dynamic changes of subunit composition. Finally, physiological implications of such regulation of subunit composition will be investigated by genetic manipulation of mice combined with electrophysiological and behavioural analyses. This work will demonstrate unprecedented views of the subunit composition in situ and provide new insights into how regulation of subunit composition contributes to synaptic plasticity and animal behaviour.

 Publications

year authors and title journal last update
List of publications.
2019 Shigekazu Tabata, Marijo Jevtic, Nobutaka Kurashige, Hirokazu Fuchida, Munetsugu Kido, Kazushi Tani, Naoki Zenmyo, Shohei Uchinomiya, Harumi Harada, Makoto Itakura, Itaru Hamachi, Ryuichi Shigemoto, Akio Ojida
Electron Microscopic Detection of Single Membrane Proteins by a Specific Chemical Labeling
published pages: , ISSN: 2589-0042, DOI: 10.1016/j.isci.2019.11.025 		iScience 2020-03-20

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