SINCHAIS SIGNED
In situ analysis of single channel subunit composition in neurons: physiological implication in synaptic plasticity and behavior
Total Cost €
0EC-Contrib. €
0Partnership
0Views
0SINCHAIS project word cloud
Explore the words cloud of the SINCHAIS project. It provides you a very rough idea of what is the project "SINCHAIS" about.
Project "SINCHAIS" data sheet
The following table provides information about the project.
| Coordinator |
INSTITUTE OF SCIENCE AND TECHNOLOGY AUSTRIA
Organization address contact info |
| Coordinator Country | Austria [AT] |
| Project website | https://ist.ac.at/research/research-groups/shigemoto-group/erc-advanced-grant/ |
| Total cost | 2˙481˙437 € |
| EC max contribution | 2˙481˙437 € (100%) |
| Programme |
1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC)) |
| Code Call | ERC-2015-AdG |
| Funding Scheme | ERC-ADG |
| Starting year | 2016 |
| Duration (year-month-day) | from 2016-07-01 to 2021-06-30 |
Partnership
Take a look of project's partnership.
| # | ||||
|---|---|---|---|---|
| 1 | INSTITUTE OF SCIENCE AND TECHNOLOGY AUSTRIA | AT (KLOSTERNEUBURG) | coordinator | 2˙481˙437.00 |
Map
Project objective
Ligand-gated and voltage-gated channels are key molecules in transforming chemical signals into electrical ones and electrical signals into chemical ones, respectively. At excitatory synaptic connections in the brain, activation of AMPA- and NMDA-type glutamate receptors elicits inward currents at the postsynaptic sites, and activation of voltage-gated calcium channels triggers vesicle release of glutamate in the presynaptic sites. Plastic changes in their number, location and property can lead to potentiation or depression of synaptic efficacy, alteration in time course, and coupling to effectors at both postsynaptic and presynaptic sites. These channels are all composed of distinct subunits and their compositions affect channel properties, trafficking to the synaptic sites, and interaction with associated molecules, creating a large diversity of synaptic functions. Although channels with different subunit compositions have been investigated using biochemical and electrophysiological detection methods, very little is known about single channel subunit composition in situ because of the lack of high resolution methods for analysis of protein complex in intact tissues. In this project, I will develop novel technologies to visualize subunit composition at the single channel level in individual synapses by electron microscopy, combining new EM tags, freeze-fracture replica labelling, and electron tomography. Synaptic plasticity will be induced by optogenetic stimulation of identified neurons or behavioural paradigms to examine the dynamic changes of subunit composition. Finally, physiological implications of such regulation of subunit composition will be investigated by genetic manipulation of mice combined with electrophysiological and behavioural analyses. This work will demonstrate unprecedented views of the subunit composition in situ and provide new insights into how regulation of subunit composition contributes to synaptic plasticity and animal behaviour.
Publications
| year | authors and title | journal | last update |
|---|---|---|---|
| 2019 |
Shigekazu Tabata, Marijo Jevtic, Nobutaka Kurashige, Hirokazu Fuchida, Munetsugu Kido, Kazushi Tani, Naoki Zenmyo, Shohei Uchinomiya, Harumi Harada, Makoto Itakura, Itaru Hamachi, Ryuichi Shigemoto, Akio Ojida Electron Microscopic Detection of Single Membrane Proteins by a Specific Chemical Labeling published pages: , ISSN: 2589-0042, DOI: 10.1016/j.isci.2019.11.025 |
iScience | 2020-03-20 |
Are you the coordinator (or a participant) of this project? Plaese send me more information about the "SINCHAIS" project.
For instance: the website url (it has not provided by EU-opendata yet), the logo, a more detailed description of the project (in plain text as a rtf file or a word file), some pictures (as picture files, not embedded into any word file), twitter account, linkedin page, etc.
Send me an email (fabio@fabiodisconzi.com) and I put them in your project's page as son as possible.
Thanks. And then put a link of this page into your project's website.
The information about "SINCHAIS" are provided by the European Opendata Portal: CORDIS opendata.