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SINCHAIS SIGNED

In situ analysis of single channel subunit composition in neurons: physiological implication in synaptic plasticity and behavior

Total Cost €

0

EC-Contrib. €

0

Partnership

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 SINCHAIS project word cloud

Explore the words cloud of the SINCHAIS project. It provides you a very rough idea of what is the project "SINCHAIS" about.

synaptic    resolution    individual    electrophysiological    freeze    connections    depression    regulation    contributes    activation    replica    elicits    visualize    chemical    tomography    location    plastic    tags    genetic    nmda    ampa    em    unprecedented    detection    transforming    coupling    implications    trafficking    dynamic    labelling    channels    glutamate    manipulation    respectively    diversity    currents    plasticity    compositions    functions    channel    effectors    electrical    triggers    intact    molecules    efficacy    interaction    composition    microscopy    mice    stimulation    postsynaptic    subunits    brain    neurons    voltage    vesicle    sites    ones    little    combined    combining    subunit    technologies    calcium    signals    gated    electron    inward    insights    synapses    protein    optogenetic    tissues    time    views    receptors    physiological    fracture    potentiation    property    situ    behavioural    ligand    presynaptic    course    lack    examine    single    animal    release    biochemical    paradigms    alteration    excitatory   

Project "SINCHAIS" data sheet

The following table provides information about the project.

Coordinator
INSTITUTE OF SCIENCE AND TECHNOLOGY AUSTRIA 

Organization address
address: Am Campus 1
city: KLOSTERNEUBURG
postcode: 3400
website: www.ist.ac.at

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Austria [AT]
 Project website https://ist.ac.at/research/research-groups/shigemoto-group/erc-advanced-grant/
 Total cost 2˙481˙437 €
 EC max contribution 2˙481˙437 € (100%)
 Programme 1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC))
 Code Call ERC-2015-AdG
 Funding Scheme ERC-ADG
 Starting year 2016
 Duration (year-month-day) from 2016-07-01   to  2021-06-30

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    INSTITUTE OF SCIENCE AND TECHNOLOGY AUSTRIA AT (KLOSTERNEUBURG) coordinator 2˙481˙437.00

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 Project objective

Ligand-gated and voltage-gated channels are key molecules in transforming chemical signals into electrical ones and electrical signals into chemical ones, respectively. At excitatory synaptic connections in the brain, activation of AMPA- and NMDA-type glutamate receptors elicits inward currents at the postsynaptic sites, and activation of voltage-gated calcium channels triggers vesicle release of glutamate in the presynaptic sites. Plastic changes in their number, location and property can lead to potentiation or depression of synaptic efficacy, alteration in time course, and coupling to effectors at both postsynaptic and presynaptic sites. These channels are all composed of distinct subunits and their compositions affect channel properties, trafficking to the synaptic sites, and interaction with associated molecules, creating a large diversity of synaptic functions. Although channels with different subunit compositions have been investigated using biochemical and electrophysiological detection methods, very little is known about single channel subunit composition in situ because of the lack of high resolution methods for analysis of protein complex in intact tissues. In this project, I will develop novel technologies to visualize subunit composition at the single channel level in individual synapses by electron microscopy, combining new EM tags, freeze-fracture replica labelling, and electron tomography. Synaptic plasticity will be induced by optogenetic stimulation of identified neurons or behavioural paradigms to examine the dynamic changes of subunit composition. Finally, physiological implications of such regulation of subunit composition will be investigated by genetic manipulation of mice combined with electrophysiological and behavioural analyses. This work will demonstrate unprecedented views of the subunit composition in situ and provide new insights into how regulation of subunit composition contributes to synaptic plasticity and animal behaviour.

 Publications

year authors and title journal last update
List of publications.
2019 Shigekazu Tabata, Marijo Jevtic, Nobutaka Kurashige, Hirokazu Fuchida, Munetsugu Kido, Kazushi Tani, Naoki Zenmyo, Shohei Uchinomiya, Harumi Harada, Makoto Itakura, Itaru Hamachi, Ryuichi Shigemoto, Akio Ojida
Electron Microscopic Detection of Single Membrane Proteins by a Specific Chemical Labeling
published pages: , ISSN: 2589-0042, DOI: 10.1016/j.isci.2019.11.025
iScience 2020-03-20

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