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In situ analysis of single channel subunit composition in neurons: physiological implication in synaptic plasticity and behavior

Total Cost €


EC-Contrib. €






 SINCHAIS project word cloud

Explore the words cloud of the SINCHAIS project. It provides you a very rough idea of what is the project "SINCHAIS" about.

respectively    visualize    channels    stimulation    ampa    connections    ones    individual    intact    em    mice    plastic    currents    signals    receptors    voltage    effectors    detection    nmda    interaction    protein    property    composition    plasticity    little    regulation    trafficking    calcium    microscopy    resolution    lack    subunit    compositions    neurons    course    brain    excitatory    location    ligand    tags    freeze    electron    electrophysiological    dynamic    implications    functions    combined    inward    animal    replica    vesicle    chemical    presynaptic    electrical    unprecedented    synaptic    potentiation    paradigms    insights    examine    synapses    depression    molecules    gated    glutamate    situ    biochemical    views    combining    channel    coupling    genetic    technologies    alteration    transforming    activation    contributes    elicits    fracture    single    optogenetic    behavioural    physiological    labelling    triggers    time    sites    tissues    postsynaptic    tomography    manipulation    subunits    diversity    release    efficacy   

Project "SINCHAIS" data sheet

The following table provides information about the project.


Organization address
address: Am Campus 1
postcode: 3400

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Austria [AT]
 Project website
 Total cost 2˙481˙437 €
 EC max contribution 2˙481˙437 € (100%)
 Programme 1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC))
 Code Call ERC-2015-AdG
 Funding Scheme ERC-ADG
 Starting year 2016
 Duration (year-month-day) from 2016-07-01   to  2021-06-30


Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 


 Project objective

Ligand-gated and voltage-gated channels are key molecules in transforming chemical signals into electrical ones and electrical signals into chemical ones, respectively. At excitatory synaptic connections in the brain, activation of AMPA- and NMDA-type glutamate receptors elicits inward currents at the postsynaptic sites, and activation of voltage-gated calcium channels triggers vesicle release of glutamate in the presynaptic sites. Plastic changes in their number, location and property can lead to potentiation or depression of synaptic efficacy, alteration in time course, and coupling to effectors at both postsynaptic and presynaptic sites. These channels are all composed of distinct subunits and their compositions affect channel properties, trafficking to the synaptic sites, and interaction with associated molecules, creating a large diversity of synaptic functions. Although channels with different subunit compositions have been investigated using biochemical and electrophysiological detection methods, very little is known about single channel subunit composition in situ because of the lack of high resolution methods for analysis of protein complex in intact tissues. In this project, I will develop novel technologies to visualize subunit composition at the single channel level in individual synapses by electron microscopy, combining new EM tags, freeze-fracture replica labelling, and electron tomography. Synaptic plasticity will be induced by optogenetic stimulation of identified neurons or behavioural paradigms to examine the dynamic changes of subunit composition. Finally, physiological implications of such regulation of subunit composition will be investigated by genetic manipulation of mice combined with electrophysiological and behavioural analyses. This work will demonstrate unprecedented views of the subunit composition in situ and provide new insights into how regulation of subunit composition contributes to synaptic plasticity and animal behaviour.


year authors and title journal last update
List of publications.
2019 Shigekazu Tabata, Marijo Jevtic, Nobutaka Kurashige, Hirokazu Fuchida, Munetsugu Kido, Kazushi Tani, Naoki Zenmyo, Shohei Uchinomiya, Harumi Harada, Makoto Itakura, Itaru Hamachi, Ryuichi Shigemoto, Akio Ojida
Electron Microscopic Detection of Single Membrane Proteins by a Specific Chemical Labeling
published pages: , ISSN: 2589-0042, DOI: 10.1016/j.isci.2019.11.025
iScience 2020-03-20

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