EU H2020 Project "SINCHAIS (In situ analysis of single channel subunit composition in neurons:..)": description, partecipants, costs and EC-fundings

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SINCHAIS project word cloud

Explore the words cloud of the SINCHAIS project. It provides you a very rough idea of what is the project "SINCHAIS" about.

excitatory location fracture ampa alteration genetic little dynamic inward potentiation postsynaptic presynaptic biochemical contributes stimulation replica physiological gated coupling combined channel regulation compositions situ synaptic ligand synapses visualize implications triggers vesicle transforming depression chemical subunits technologies voltage manipulation views freeze electrical unprecedented currents neurons subunit trafficking time combining composition insights signals examine tags property glutamate calcium brain optogenetic protein sites tissues diversity effectors ones channels interaction tomography resolution functions plasticity efficacy connections plastic mice electron intact release single microscopy animal activation elicits electrophysiological em paradigms individual receptors lack nmda detection course respectively behavioural molecules labelling

Project "SINCHAIS" data sheet

The following table provides information about the project.

Coordinator	INSTITUTE OF SCIENCE AND TECHNOLOGY AUSTRIA Organization address address: Am Campus 1 city: KLOSTERNEUBURG postcode: 3400 website: www.ist.ac.at contact info title: n.a. name: n.a. surname: n.a. function: n.a. email: n.a. telephone: n.a. fax: n.a.
Coordinator Country	Austria [AT]
Project website	https://ist.ac.at/research/research-groups/shigemoto-group/erc-advanced-grant/
Total cost	2˙481˙437 €
EC max contribution	2˙481˙437 € (100%)
Programme	1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC))
Code Call	ERC-2015-AdG
Funding Scheme	ERC-ADG
Starting year	2016
Duration (year-month-day)	from 2016-07-01 to 2021-06-30

#	participants	country	role	EC contrib. [€]
1	INSTITUTE OF SCIENCE AND TECHNOLOGY AUSTRIA	AT (KLOSTERNEUBURG)	coordinator	2˙481˙437.00

INSTITUTE OF SCIENCE AND TECHNOLOGY AUSTRIA

AT (KLOSTERNEUBURG)

coordinator

2˙481˙437.00

Project objective

Ligand-gated and voltage-gated channels are key molecules in transforming chemical signals into electrical ones and electrical signals into chemical ones, respectively. At excitatory synaptic connections in the brain, activation of AMPA- and NMDA-type glutamate receptors elicits inward currents at the postsynaptic sites, and activation of voltage-gated calcium channels triggers vesicle release of glutamate in the presynaptic sites. Plastic changes in their number, location and property can lead to potentiation or depression of synaptic efficacy, alteration in time course, and coupling to effectors at both postsynaptic and presynaptic sites. These channels are all composed of distinct subunits and their compositions affect channel properties, trafficking to the synaptic sites, and interaction with associated molecules, creating a large diversity of synaptic functions. Although channels with different subunit compositions have been investigated using biochemical and electrophysiological detection methods, very little is known about single channel subunit composition in situ because of the lack of high resolution methods for analysis of protein complex in intact tissues. In this project, I will develop novel technologies to visualize subunit composition at the single channel level in individual synapses by electron microscopy, combining new EM tags, freeze-fracture replica labelling, and electron tomography. Synaptic plasticity will be induced by optogenetic stimulation of identified neurons or behavioural paradigms to examine the dynamic changes of subunit composition. Finally, physiological implications of such regulation of subunit composition will be investigated by genetic manipulation of mice combined with electrophysiological and behavioural analyses. This work will demonstrate unprecedented views of the subunit composition in situ and provide new insights into how regulation of subunit composition contributes to synaptic plasticity and animal behaviour.

Publications

List of publications.
year	authors and title	journal	last update
2019	Shigekazu Tabata, Marijo Jevtic, Nobutaka Kurashige, Hirokazu Fuchida, Munetsugu Kido, Kazushi Tani, Naoki Zenmyo, Shohei Uchinomiya, Harumi Harada, Makoto Itakura, Itaru Hamachi, Ryuichi Shigemoto, Akio Ojida Electron Microscopic Detection of Single Membrane Proteins by a Specific Chemical Labeling published pages: , ISSN: 2589-0042, DOI: 10.1016/j.isci.2019.11.025	iScience	2020-03-20

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