EU H2020 Project "RAPID (Chromatin dynamics resolved by rapid protein labeling and bioorthogonal..)": description, partecipants, costs and EC-fundings

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RAPID project word cloud

Explore the words cloud of the RAPID project. It provides you a very rough idea of what is the project "RAPID" about.

evolution cell chase rapid developmentally domains descriptions resolved modulated modification spectrometry packaging pulse flexible regulated mouse form minutes pioneers fundamental collect dynamics linked coupled relative division chip stem stability gene population selective propensity microscopy mesc little epigenomics period specification integrates mass memory capturing sequencing inheritance histone experiments replication epigenetic seq pluripotent heterochromatin embryonic employ occupancy eukaryotic propagation dimensional cycle proteins heritable protein lineage polycomb readouts genetics functionally time signals fast maintained labeling cellular snapshots suited stable interstitial multitude chromatin sensitive pluripotency dynamic gen model days cells uniquely rules genome landscape epigenomic termed dimension expression combination ranging introduces marks

Project "RAPID" data sheet

The following table provides information about the project.

Coordinator	KAROLINSKA INSTITUTET Organization address address: Nobels Vag 5 city: STOCKHOLM postcode: 17177 website: www.ki.se contact info title: n.a. name: n.a. surname: n.a. function: n.a. email: n.a. telephone: n.a. fax: n.a.
Coordinator Country	Sweden [SE]
Total cost	1˙846˙360 €
EC max contribution	1˙846˙360 € (100%)
Programme	1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC))
Code Call	ERC-2016-STG
Funding Scheme	ERC-STG
Starting year	2017
Duration (year-month-day)	from 2017-01-01 to 2021-12-31

#	participants	country	role	EC contrib. [€]
1	KAROLINSKA INSTITUTET	SE (STOCKHOLM)	coordinator	1˙846˙360.00

KAROLINSKA INSTITUTET

SE (STOCKHOLM)

coordinator

1˙846˙360.00

Project objective

Histone proteins provide a dynamic packaging system for the eukaryotic genome. Chromatin integrates a multitude of signals to control gene expression, only some of which have the propensity to be maintained through replication and cell division. For our understanding of cellular memory and epigenetic inheritance we need to know what features characterize a stable, heritable chromatin state throughout the cell cycle. State-of-the-art methods such as ChIP-Seq provide population-based snapshots of the epigenomic landscape but little information on the stability and relative importance of each studied feature or modification. This project pioneers a rapid, sensitive and selective protein labeling method (termed RAPID) for capturing genome-wide chromatin dynamics resolved over a period of time ranging from minutes to days. RAPID introduces a flexible time dimension in the form of pulse or pulse-chase experiments for studying genome-wide occupancy of a protein of interest by next-gen sequencing. It can also be coupled to other readouts such as mass spectrometry or microscopy. RAPID is uniquely suited for studying cell cycle-linked processes, by defining when and where stable ‘marks’ are set in chromatin. I will employ mouse embryonic stem cell (mESC) as a model system for pluripotency and lineage specification. RAPID will define fundamental rules for inheritance of histone and other chromatin-associated proteins and how they are modulated by the fast cell cycle of pluripotent cells. Using RAPID in combination with other state-of-the art genetics and epigenomics, I will collect multi-dimensional descriptions of the dynamic evolution and propagation of functionally relevant chromatin states, such as interstitial heterochromatin and developmentally regulated Polycomb domains.

Publications

List of publications.
year	authors and title	journal	last update
2018	Birthe Meineke, Johannes HeimgÃ¤rtner, Lorenzo Lafranchi, Simon J ElsÃ¤sser Methanomethylophilus alvus Mx1201 provides basis for mutual orthogonal pyrrolysyl tRNA/aminoacyl-tRNA synthetase pairs in mammalian cells published pages: , ISSN: , DOI: 10.1101/371757	BioRxiv Preprint	2019-06-13
2019	Banushree Kumar, Simon J ElsÃ¤sser Quantitative multiplexed ChIP reveals global alterations that shape promoter bivalency in ground state embryonic stem cells published pages: , ISSN: , DOI: 10.1101/557082	BioRxiv Preprint	2019-08-29
2018	Birthe Meineke, Johannes HeimgÃ¤rtner, Lorenzo Lafranchi, Simon J. ElsÃ¤sser Methanomethylophilus alvus Mx1201 Provides Basis for Mutual Orthogonal Pyrrolysyl tRNA/Aminoacyl-tRNA Synthetase Pairs in Mammalian Cells published pages: 3087-3096, ISSN: 1554-8929, DOI: 10.1021/acschembio.8b00571	ACS Chemical Biology 13/11	2019-08-29

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