Opendata, web and dolomites

Lightsheetelegans

In-toto imaging of C. elegans larval development using adaptive optics light-sheet microscopy

Total Cost €

0

EC-Contrib. €

0

Partnership

0

Views

0

 Lightsheetelegans project word cloud

Explore the words cloud of the Lightsheetelegans project. It provides you a very rough idea of what is the project "Lightsheetelegans" about.

behavioral    microscope    lack    temporal    elegans    caused    brain    engineering    isotropic    laser    chips    ideally    toto    reconstructions    chamber    physics    alternative    rewired    ablations    critical    animals    microscopes    geared    survival    adaptive    gap    our    tissue    nematode    material    behavior    media    conventional    nervous    fundamental    suited    mouse    refers    period    electron    aberrations    minimize    question    compatible    stage    parallel    optical    spatio    rewiring    little    versatile    dauer    developmental    larval    embryo    manipulations    imaging    neuroscience    scanning    anatomy    exiting    organisms    zebrafish    plasticity    limitation    candidate    relatively    entirety    optics    activation    active    biology    larva    causal    translucent    combine    neuronal    sheet    samples    technique    light    body    larger    mechanisms    adult    unpublished    larvae    probe    microscopy    resolution    microfluidic    roles    diapause    routinely    stereotypical    fast    transitions    transformations    refractive    animal    pave    model    genetics    optogenetic    data    thick    neurons   

Project "Lightsheetelegans" data sheet

The following table provides information about the project.

Coordinator
MAX DELBRUECK CENTRUM FUER MOLEKULARE MEDIZIN IN DER HELMHOLTZ-GEMEINSCHAFT (MDC) 

Organization address
address: ROBERT ROSSLE STRASSE 10
city: BERLIN
postcode: 13125
website: www.mdc-berlin.de

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Germany [DE]
 Total cost 171˙460 €
 EC max contribution 171˙460 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2016
 Funding Scheme MSCA-IF-EF-ST
 Starting year 2017
 Duration (year-month-day) from 2017-03-01   to  2019-03-31

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    MAX DELBRUECK CENTRUM FUER MOLEKULARE MEDIZIN IN DER HELMHOLTZ-GEMEINSCHAFT (MDC) DE (BERLIN) coordinator 171˙460.00

Map

 Project objective

Our project aims to combine concepts and methodologies from biology, physics and engineering to address a fundamental question in neuroscience: how is the nervous system rewired during development transitions. The nematode C. elegans is one of the most important model organisms in neuroscience due to its relatively simple stereotypical anatomy, well-studied genetics and behavior, translucent body and nervous system, ideally suited for in-toto (in its entirety) imaging. Yet most of the knowledge available refers to adult animals, and little is known about neuronal plasticity and mechanisms of behavioral changes during development. One of the technical reasons for this gap is the lack of high-resolution fast-scanning optical microscopes compatible with microfluidic devices that are routinely used in nematode larva studies. To address this limitation, we propose to build a light-sheet microscope for imaging C. elegans larvae in conventional microfluidic chips with high spatio-temporal resolution, and minimize the optical aberrations caused by chamber material using adaptive optics. The microscope will allow parallel imaging of larvae while exiting dauer diapause (an alternative larval stage geared for survival). We will use the imaging data together with available (yet unpublished) electron microscopy reconstructions of C. elegans dauer larva to study nervous system rewiring during this critical period of animal development, and which neurons are active during developmental changes. This information will pave the way for optical manipulations of candidate neurons by laser ablations and optogenetic activation, to probe their causal roles in developmental transformations. Due to its versatile design, our microscopy technique may be further applied in other studies where high-resolution imaging through non-isotropic refractive media is required, such as imaging through thick brain tissue samples, or in larger model organisms (e.g. zebrafish larva, mouse embryo).

Are you the coordinator (or a participant) of this project? Plaese send me more information about the "LIGHTSHEETELEGANS" project.

For instance: the website url (it has not provided by EU-opendata yet), the logo, a more detailed description of the project (in plain text as a rtf file or a word file), some pictures (as picture files, not embedded into any word file), twitter account, linkedin page, etc.

Send me an  email (fabio@fabiodisconzi.com) and I put them in your project's page as son as possible.

Thanks. And then put a link of this page into your project's website.

The information about "LIGHTSHEETELEGANS" are provided by the European Opendata Portal: CORDIS opendata.

More projects from the same programme (H2020-EU.1.3.2.)

Widow Spider Mating (2020)

Immature mating as a novel tactic of an invasive widow spider

Read More  

ASIQS (2019)

Antiferromagnetic spintronics investigated by quantum sensing techniques

Read More  

SymCO (2020)

Asymptotic Symmetries: from Concepts to Observations

Read More