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Lightsheetelegans

In-toto imaging of C. elegans larval development using adaptive optics light-sheet microscopy

Total Cost €

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EC-Contrib. €

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Partnership

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 Lightsheetelegans project word cloud

Explore the words cloud of the Lightsheetelegans project. It provides you a very rough idea of what is the project "Lightsheetelegans" about.

plasticity    translucent    developmental    thick    model    adaptive    mouse    biology    caused    material    sheet    exiting    brain    animal    limitation    larva    our    active    laser    fast    elegans    conventional    refractive    candidate    relatively    technique    combine    diapause    scanning    neuronal    microscope    parallel    organisms    body    larvae    larger    optics    adult    genetics    samples    behavioral    aberrations    unpublished    larval    microscopy    stage    optogenetic    versatile    spatio    gap    compatible    zebrafish    optical    ablations    transformations    resolution    electron    period    behavior    alternative    rewiring    toto    embryo    ideally    mechanisms    question    suited    isotropic    anatomy    reconstructions    chamber    nematode    routinely    physics    transitions    neurons    temporal    activation    geared    pave    stereotypical    media    probe    tissue    neuroscience    survival    little    manipulations    engineering    roles    imaging    light    critical    fundamental    chips    lack    rewired    minimize    data    causal    microscopes    dauer    microfluidic    animals    entirety    nervous    refers   

Project "Lightsheetelegans" data sheet

The following table provides information about the project.

Coordinator
MAX DELBRUECK CENTRUM FUER MOLEKULARE MEDIZIN IN DER HELMHOLTZ-GEMEINSCHAFT (MDC) 

Organization address
address: ROBERT ROSSLE STRASSE 10
city: BERLIN
postcode: 13125
website: www.mdc-berlin.de

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Germany [DE]
 Total cost 171˙460 €
 EC max contribution 171˙460 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2016
 Funding Scheme MSCA-IF-EF-ST
 Starting year 2017
 Duration (year-month-day) from 2017-03-01   to  2019-03-31

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    MAX DELBRUECK CENTRUM FUER MOLEKULARE MEDIZIN IN DER HELMHOLTZ-GEMEINSCHAFT (MDC) DE (BERLIN) coordinator 171˙460.00

Map

 Project objective

Our project aims to combine concepts and methodologies from biology, physics and engineering to address a fundamental question in neuroscience: how is the nervous system rewired during development transitions. The nematode C. elegans is one of the most important model organisms in neuroscience due to its relatively simple stereotypical anatomy, well-studied genetics and behavior, translucent body and nervous system, ideally suited for in-toto (in its entirety) imaging. Yet most of the knowledge available refers to adult animals, and little is known about neuronal plasticity and mechanisms of behavioral changes during development. One of the technical reasons for this gap is the lack of high-resolution fast-scanning optical microscopes compatible with microfluidic devices that are routinely used in nematode larva studies. To address this limitation, we propose to build a light-sheet microscope for imaging C. elegans larvae in conventional microfluidic chips with high spatio-temporal resolution, and minimize the optical aberrations caused by chamber material using adaptive optics. The microscope will allow parallel imaging of larvae while exiting dauer diapause (an alternative larval stage geared for survival). We will use the imaging data together with available (yet unpublished) electron microscopy reconstructions of C. elegans dauer larva to study nervous system rewiring during this critical period of animal development, and which neurons are active during developmental changes. This information will pave the way for optical manipulations of candidate neurons by laser ablations and optogenetic activation, to probe their causal roles in developmental transformations. Due to its versatile design, our microscopy technique may be further applied in other studies where high-resolution imaging through non-isotropic refractive media is required, such as imaging through thick brain tissue samples, or in larger model organisms (e.g. zebrafish larva, mouse embryo).

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The information about "LIGHTSHEETELEGANS" are provided by the European Opendata Portal: CORDIS opendata.

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