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Lightsheetelegans

In-toto imaging of C. elegans larval development using adaptive optics light-sheet microscopy

Total Cost €

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EC-Contrib. €

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Partnership

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 Lightsheetelegans project word cloud

Explore the words cloud of the Lightsheetelegans project. It provides you a very rough idea of what is the project "Lightsheetelegans" about.

rewiring    animals    microscope    microscopy    optical    limitation    candidate    relatively    exiting    transformations    thick    embryo    translucent    spatio    causal    ablations    refers    mechanisms    suited    chamber    tissue    anatomy    manipulations    stereotypical    lack    minimize    probe    samples    conventional    scanning    roles    diapause    brain    neuroscience    isotropic    developmental    refractive    nervous    physics    engineering    media    survival    behavior    electron    material    elegans    pave    optogenetic    entirety    sheet    neuronal    data    fast    alternative    genetics    mouse    critical    plasticity    activation    little    imaging    versatile    body    neurons    stage    behavioral    laser    organisms    toto    larvae    temporal    adult    larval    parallel    chips    geared    aberrations    biology    microfluidic    nematode    model    unpublished    dauer    microscopes    active    zebrafish    rewired    compatible    resolution    fundamental    transitions    period    reconstructions    animal    larger    light    our    combine    caused    ideally    routinely    larva    question    technique    adaptive    optics    gap   

Project "Lightsheetelegans" data sheet

The following table provides information about the project.

Coordinator
MAX DELBRUECK CENTRUM FUER MOLEKULARE MEDIZIN IN DER HELMHOLTZ-GEMEINSCHAFT (MDC) 

Organization address
address: ROBERT ROSSLE STRASSE 10
city: BERLIN
postcode: 13125
website: www.mdc-berlin.de

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Germany [DE]
 Total cost 171˙460 €
 EC max contribution 171˙460 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2016
 Funding Scheme MSCA-IF-EF-ST
 Starting year 2017
 Duration (year-month-day) from 2017-03-01   to  2019-03-31

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    MAX DELBRUECK CENTRUM FUER MOLEKULARE MEDIZIN IN DER HELMHOLTZ-GEMEINSCHAFT (MDC) DE (BERLIN) coordinator 171˙460.00

Map

 Project objective

Our project aims to combine concepts and methodologies from biology, physics and engineering to address a fundamental question in neuroscience: how is the nervous system rewired during development transitions. The nematode C. elegans is one of the most important model organisms in neuroscience due to its relatively simple stereotypical anatomy, well-studied genetics and behavior, translucent body and nervous system, ideally suited for in-toto (in its entirety) imaging. Yet most of the knowledge available refers to adult animals, and little is known about neuronal plasticity and mechanisms of behavioral changes during development. One of the technical reasons for this gap is the lack of high-resolution fast-scanning optical microscopes compatible with microfluidic devices that are routinely used in nematode larva studies. To address this limitation, we propose to build a light-sheet microscope for imaging C. elegans larvae in conventional microfluidic chips with high spatio-temporal resolution, and minimize the optical aberrations caused by chamber material using adaptive optics. The microscope will allow parallel imaging of larvae while exiting dauer diapause (an alternative larval stage geared for survival). We will use the imaging data together with available (yet unpublished) electron microscopy reconstructions of C. elegans dauer larva to study nervous system rewiring during this critical period of animal development, and which neurons are active during developmental changes. This information will pave the way for optical manipulations of candidate neurons by laser ablations and optogenetic activation, to probe their causal roles in developmental transformations. Due to its versatile design, our microscopy technique may be further applied in other studies where high-resolution imaging through non-isotropic refractive media is required, such as imaging through thick brain tissue samples, or in larger model organisms (e.g. zebrafish larva, mouse embryo).

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The information about "LIGHTSHEETELEGANS" are provided by the European Opendata Portal: CORDIS opendata.

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