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Lightsheetelegans

In-toto imaging of C. elegans larval development using adaptive optics light-sheet microscopy

Total Cost €

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EC-Contrib. €

0

Partnership

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 Lightsheetelegans project word cloud

Explore the words cloud of the Lightsheetelegans project. It provides you a very rough idea of what is the project "Lightsheetelegans" about.

elegans    optics    body    suited    mouse    stereotypical    versatile    scanning    microscopes    rewiring    organisms    light    fast    neuroscience    refractive    routinely    translucent    biology    ideally    combine    refers    chamber    larvae    nervous    candidate    transformations    optical    geared    conventional    behavioral    fundamental    relatively    lack    ablations    microscope    isotropic    nematode    zebrafish    adult    transitions    stage    chips    critical    samples    animal    neurons    survival    animals    resolution    embryo    behavior    manipulations    electron    compatible    developmental    microscopy    physics    data    probe    larva    exiting    temporal    dauer    optogenetic    little    our    genetics    pave    neuronal    larger    rewired    laser    model    engineering    parallel    toto    diapause    thick    technique    causal    brain    unpublished    gap    question    limitation    adaptive    mechanisms    tissue    active    aberrations    alternative    imaging    reconstructions    activation    period    microfluidic    caused    minimize    spatio    roles    media    entirety    larval    material    sheet    plasticity    anatomy   

Project "Lightsheetelegans" data sheet

The following table provides information about the project.

Coordinator
MAX DELBRUECK CENTRUM FUER MOLEKULARE MEDIZIN IN DER HELMHOLTZ-GEMEINSCHAFT (MDC) 

Organization address
address: ROBERT ROSSLE STRASSE 10
city: BERLIN
postcode: 13125
website: www.mdc-berlin.de

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Germany [DE]
 Total cost 171˙460 €
 EC max contribution 171˙460 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2016
 Funding Scheme MSCA-IF-EF-ST
 Starting year 2017
 Duration (year-month-day) from 2017-03-01   to  2019-03-31

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    MAX DELBRUECK CENTRUM FUER MOLEKULARE MEDIZIN IN DER HELMHOLTZ-GEMEINSCHAFT (MDC) DE (BERLIN) coordinator 171˙460.00

Map

 Project objective

Our project aims to combine concepts and methodologies from biology, physics and engineering to address a fundamental question in neuroscience: how is the nervous system rewired during development transitions. The nematode C. elegans is one of the most important model organisms in neuroscience due to its relatively simple stereotypical anatomy, well-studied genetics and behavior, translucent body and nervous system, ideally suited for in-toto (in its entirety) imaging. Yet most of the knowledge available refers to adult animals, and little is known about neuronal plasticity and mechanisms of behavioral changes during development. One of the technical reasons for this gap is the lack of high-resolution fast-scanning optical microscopes compatible with microfluidic devices that are routinely used in nematode larva studies. To address this limitation, we propose to build a light-sheet microscope for imaging C. elegans larvae in conventional microfluidic chips with high spatio-temporal resolution, and minimize the optical aberrations caused by chamber material using adaptive optics. The microscope will allow parallel imaging of larvae while exiting dauer diapause (an alternative larval stage geared for survival). We will use the imaging data together with available (yet unpublished) electron microscopy reconstructions of C. elegans dauer larva to study nervous system rewiring during this critical period of animal development, and which neurons are active during developmental changes. This information will pave the way for optical manipulations of candidate neurons by laser ablations and optogenetic activation, to probe their causal roles in developmental transformations. Due to its versatile design, our microscopy technique may be further applied in other studies where high-resolution imaging through non-isotropic refractive media is required, such as imaging through thick brain tissue samples, or in larger model organisms (e.g. zebrafish larva, mouse embryo).

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The information about "LIGHTSHEETELEGANS" are provided by the European Opendata Portal: CORDIS opendata.

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