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CellTrack SIGNED

Cellular Position Tracking Using DNA Origami Barcodes

Total Cost €

0

EC-Contrib. €

0

Partnership

0

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 CellTrack project word cloud

Explore the words cloud of the CellTrack project. It provides you a very rough idea of what is the project "CellTrack" about.

locations    creation    uncover    combined    sequences    optical    cloning    mouse    spatial    previously    unknown    made    position    imaging    molecular    issue    scaffold    positions    data    physiological    origami    resolution    progenitors    differentiation    de    samples    technique    nature    deep    newly    transcriptomics    dna    resolved    conjugated    developmental    multiple    sequencing    points    readable    organ    differentiated    441    2015    mrna    barcodes    nano    display    dimensional    interdependencies    stable    crypt    hybridization    benson    vivo    transcriptome    al    strategy    structures    folding    opens    stem    gut    combinatorial    patterns    exact    mapping    biological    cancer    et    origin    turning    cell    expose    cancers    paths    time    regeneration    cells    single    procedure    theme    nucleotides    completely    proliferating    organs    523    perform    salt    types    follicle    fluorophores    tissue    effect    collected    intestine    small    spatiotemporal    hair    dependencies    expression    skin    biology    create   

Project "CellTrack" data sheet

The following table provides information about the project.

Coordinator
KAROLINSKA INSTITUTET 

Organization address
address: Nobels Vag 5
city: STOCKHOLM
postcode: 17177
website: www.ki.se

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Sweden [SE]
 Total cost 1˙923˙262 €
 EC max contribution 1˙923˙262 € (100%)
 Programme 1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC))
 Code Call ERC-2016-COG
 Funding Scheme ERC-COG
 Starting year 2017
 Duration (year-month-day) from 2017-08-01   to  2022-07-31

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    KAROLINSKA INSTITUTET SE (STOCKHOLM) coordinator 1˙923˙262.00

Map

 Project objective

The research I propose here will provide an enabling technology; spatially resolved transcriptomics, to address important problems in cell- and developmental-biology, in particular: How are stem cells in the skin and gut proliferating without turning into cancers? How are differentiated cells related, in their transcriptome and spatial positions, to their progenitors?

To investigate these problems on a molecular level and open up paths to find completely new spatiotemporal interdependencies in complex biological systems, I propose to use our newly developed DNA-origami strategy (Benson et al, Nature, 523 p. 441 (2015) ), combined with a combinatorial cloning technique, to build a new method for deep mRNA sequencing of tissue with single-cell resolution. These new types of origami are stable in physiological salt conditions and opens up their use in in-vivo applications.

In DNA-origami we can control the exact spatial position of all nucleotides. By folding the scaffold to display sequences for hybridization of fluorophores conjugated to DNA, we can create optical nano-barcodes. By using structures made out of DNA, the patterns of the optical barcodes will be readable both by imaging and by sequencing, thus enabling the creation of a mapping between cell locations in an organ and the mRNA expression of those cells.

We will use the method to perform spatially resolved transcriptomics in small organs: the mouse hair follicle, and small intestine crypt, and also perform the procedure for multiple samples collected at different time points. This will enable a high-dimensional data analysis that most likely will expose previously unknown dependencies that would provide completely new knowledge about how these biological systems work. By studying these systems, we will uncover much more information on how stem cells contribute to regeneration, the issue of de-differentiation that is a common theme in these organs and the effect this might have on the origin of cancer.

 Publications

year authors and title journal last update
List of publications.
2018 Erik Benson, Abdulmelik Mohammed, Daniel Rayneau-Kirkhope, Andreas Gådin, Pekka Orponen, Björn Högberg
Effects of Design Choices on the Stiffness of Wireframe DNA Origami Structures
published pages: 9291-9299, ISSN: 1936-0851, DOI: 10.1021/acsnano.8b04148
ACS Nano 12/9 2019-08-30

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The information about "CELLTRACK" are provided by the European Opendata Portal: CORDIS opendata.

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