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NoiseRobustEvo SIGNED

Noise and robustness in the evolution of novel protein phenotypes

Total Cost €

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EC-Contrib. €

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Partnership

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 NoiseRobustEvo project word cloud

Explore the words cloud of the NoiseRobustEvo project. It provides you a very rough idea of what is the project "NoiseRobustEvo" about.

revolutionized    mistranslation    dynamics    driving    modeling    proteins    cell    organisms    stochastic    earlier    separately    sequencing    coli    biologically    light    rates    accelerate    engineers    hinder    living    observations    duplication    hypothesize    chaperone    themselves    source    evolution    fps    data    quiet    colors    strains    emitting    ubiquitous    stabilizing    noise    robustness    acids    laboratory    mutations    revolutionize    platform    cells    barraged    fast    theory    pervasive    constantly    combination    evolve    kinds    misincorporation    revealing    molecular    originate    adaptive    hypothesis    evolutionary    transcript    molecules    engineering    predicts    directed    poorly    expression    variation    throughput    noisy    persistence    phenotypes    gene    abundant    ways    color    functions    first    jointly    amino    dna    discover    validate    analyze    synthesis    phenotypic    biology    levels    perturbations    host    protein    fluorescent    overexpression    promoters    innovation    ribosomes    manipulate   

Project "NoiseRobustEvo" data sheet

The following table provides information about the project.

Coordinator
UNIVERSITAT ZURICH 

Organization address
address: RAMISTRASSE 71
city: Zürich
postcode: 8006
website: http://www.unizh.ch

contact info
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name: n.a.
surname: n.a.
function: n.a.
email: n.a.
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 Coordinator Country Switzerland [CH]
 Total cost 2˙383˙444 €
 EC max contribution 2˙383˙444 € (100%)
 Programme 1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC))
 Code Call ERC-2016-ADG
 Funding Scheme ERC-ADG
 Starting year 2017
 Duration (year-month-day) from 2017-10-01   to  2022-09-30

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    UNIVERSITAT ZURICH CH (Zürich) coordinator 2˙383˙444.00

Map

 Project objective

Living cells are constantly barraged by perturbations that originate within themselves. Especially abundant – far more than DNA mutations – are two kinds of such perturbations. The first is gene expression noise, pervasive stochastic variation of transcript and protein levels. The second is mistranslation noise, the misincorporation of amino acids by ribosomes during protein synthesis. Organisms and protein molecules can evolve robustness – the persistence of well-adapted phenotypes – to both kinds of noise. Theory predicts that noise and robustness can affect the adaptive evolution of new proteins, but we do not know whether they help or hinder adaptive evolution. We hypothesize that noise and robustness can accelerate protein evolution both separately and jointly. To validate this hypothesis, we will evolve light-emitting fluorescent proteins towards new color phenotypes via directed laboratory evolution in E.coli. During evolution, we will manipulate expression noise by driving FP expression from noisy or quiet promoters, and we will manipulate mistranslation via host strains with low and high mistranslation rates. We will manipulate protein robustness in three biologically important ways, chaperone overexpression, gene duplication, and stabilizing selection. We will study how fast FPs evolve new colors, and analyze protein evolutionary dynamics through a combination of high-throughput sequencing, engineering of selected adaptive mutations, and data-driven modeling. Our project will show how a ubiquitous but poorly understood source of phenotypic variation affects protein innovation. It will also help engineers discover new protein functions. Moreover, our work will help establish FPs as a major platform to study protein evolutionary dynamics. By revealing noise as a new and crucial factor in protein evolution, our observations have the potential to revolutionize molecular evolution research, much like earlier studies of noise have revolutionized cell biology.

 Publications

year authors and title journal last update
List of publications.
2019 Jia Zheng, Joshua L. Payne, Andreas Wagner
Cryptic genetic variation accelerates evolution by opening access to diverse adaptive peaks
published pages: 347-353, ISSN: 0036-8075, DOI: 10.1126/science.aax1837
Science 365/6451 2020-04-15
2019 Aguilar-Rodríguez, José; Fares, Mario A; Wagner, Andreas
Chaperonin overproduction and metabolic erosion caused by mutation accumulation in Escherichia coli
published pages: , ISSN: 0378-1097, DOI: 10.5167/uzh-182142
Aguilar-Rodríguez, José; Fares, Mario A; Wagner, Andreas (2019). Chaperonin overproduction and metabolic erosion caused by mutation accumulation in Escherichia coli. FEMS Microbiology Letters, 366(10):fnz121. 1 2020-04-15
2019 Joshua L. Payne, Andreas Wagner
The causes of evolvability and their evolution
published pages: 24-38, ISSN: 1471-0056, DOI: 10.1038/s41576-018-0069-z
Nature Reviews Genetics 20/1 2020-04-15
2018 Payne, Joshua L; Khalid, Fahad; Wagner, Andreas
RNA-mediated gene regulation is less evolvable than transcriptional regulation
published pages: , ISSN: 1091-6490, DOI: 10.5167/uzh-167214
PNAS 3 2019-06-06
2018 Aguilar-Rodríguez, José; Peel, Leto; Stella, Massimo; Wagner, Andreas; Payne, Joshua L.
The architecture of an empirical genotype-phenotype map
published pages: , ISSN: 1558-5646, DOI: 10.3929/ethz-b-000274406
Evolution, 72 (6) 11 2019-06-06
2018 Kathleen Sprouffske, José Aguilar-Rodríguez, Paul Sniegowski, Andreas Wagner
High mutation rates limit evolutionary adaptation in Escherichia coli
published pages: e1007324, ISSN: 1553-7404, DOI: 10.1371/journal.pgen.1007324
PLOS Genetics 14/4 2019-06-06

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