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PlasmaCellControl SIGNED

Transcriptional control of plasma cell development and function

Total Cost €

EC-Contrib. €

Partnership

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PlasmaCellControl project word cloud

Explore the words cloud of the PlasmaCellControl project. It provides you a very rough idea of what is the project "PlasmaCellControl" about.

molecular loxp retrovirus mediated blimp1 pathogens function fundamentally rosa26 infected crispr cas9 infection validation humoral xbp1 unknown extend optimized antibody transcription phenotypes interesting conditional systematically implicated aiolos acute ikaros vivo newly potentially experiment proliferating irf4 showed retroviruses quiescent lived mutant proteins upregulated protocols proof seq individual rna immunity recapitulated host cell secreting performed hits lines transcriptional regulators bhlha15 players plasmablasts vitro discover followed protection expressing sgrna impaired differentiation ranked effector cells cre functions plasmablast few screens exception genes consisting experiments deletion plasma regulated pooled mechanisms libraries mutagenesis chip

Project "PlasmaCellControl" data sheet

The following table provides information about the project.

Coordinator	FORSCHUNGSINSTITUT FUR MOLEKULARE PATHOLOGIE GESELLSCHAFT MBH Organization address address: CAMPUS-VIENNA-BIOCENTER 1 city: WIEN postcode: 1030 website: www.imp.ac.at contact info title: n.a. name: n.a. surname: n.a. function: n.a. email: n.a. telephone: n.a. fax: n.a.
Coordinator Country	Austria [AT]
Total cost	2˙500˙000 €
EC max contribution	2˙500˙000 € (100%)
Programme	1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC))
Code Call	ERC-2016-ADG
Funding Scheme	ERC-ADG
Starting year	2018
Duration (year-month-day)	from 2018-01-01 to 2022-12-31

Partnership

Take a look of project's partnership.

#	participants	country	role	EC contrib. [€]
1	FORSCHUNGSINSTITUT FUR MOLEKULARE PATHOLOGIE GESELLSCHAFT MBH FORSCHUNGSINSTITUT FUR MOLEKULARE PATHOLOGIE GESELLSCHAFT MBH Organization address address: CAMPUS-VIENNA-BIOCENTER 1 city: WIEN postcode: 1030 website: www.imp.ac.at contact info title: n.a. name: n.a. surname: n.a. function: n.a. email: n.a. telephone: n.a. fax: n.a.	AT (WIEN)	coordinator	2˙500˙000.00

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Project objective

Antibody-secreting cells consisting of short-lived proliferating plasmablasts and long-lived quiescent plasma cells are essential for the acute response to infection and long-term protection of the host against pathogens. Only a few regulators (Blimp1, IRF4, XBP1, Aiolos, Ikaros and E-proteins) have been implicated in the transcriptional control of antibody-secreting cells, and their target genes, with the exception of Blimp1 and E-proteins, are still unknown. This proposal aims to systematically identify key players in the development and function of antibody-secreting cells by using the CRISPR/Cas9 and Cre/loxP methods. For this, we improved existing protocols to extend the duration of in vitro plasmablast differentiation and showed that Rosa26(Cas9/) B cells infected with Blimp1 or Xbp1 sgRNA-expressing retroviruses recapitulated the Blimp1 and Xbp1 mutant phenotypes in this proof-of-principle experiment. Moreover, Cre retrovirus-mediated deletion of Irf4, Ikaros and Aiolos strongly impaired plasmablast differentiation in this optimized system. To discover new regulators of plasma cell differentiation, CRISPR/Cas9-based screens will be performed with pooled sgRNA libraries targeting all known upregulated genes in plasmablasts and plasma cells, followed by individual validation of the best hits. Selected top-ranked genes will be analyzed in vivo by conditional mutagenesis with newly generated, plasma cell-specific Cre lines. Regulated target genes of IRF4, Ikaros, Aiolos, XBP1 and the XBP1-regulated transcription factor Bhlha15 will be identified in plasmablasts by ChIP- and RNA-seq analyses. Target genes with potentially interesting functions will be further characterized by CRISPR/Cas9- or Cre/loxP-mediated mutagenesis. These experiments will provide fundamentally new insight into the molecular mechanisms controlling the development and function of antibody-secreting cells, which are the essential effector cells of humoral immunity.

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The information about "PLASMACELLCONTROL" are provided by the European Opendata Portal: CORDIS opendata.