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LD_Biogenesis SIGNED

Identification and characterization of sites of lipid droplet biogenesis in the ER

Total Cost €

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EC-Contrib. €

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Partnership

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Project "LD_Biogenesis" data sheet

The following table provides information about the project.

Coordinator
UNIVERSITE DE FRIBOURG 

Organization address
address: AVENUE DE L EUROPE 20
city: FRIBOURG
postcode: 1700
website: www.unifr.ch

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Switzerland [CH]
 Total cost 187˙419 €
 EC max contribution 187˙419 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2016
 Funding Scheme MSCA-IF-EF-RI
 Starting year 2018
 Duration (year-month-day) from 2018-03-01   to  2020-02-29

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    UNIVERSITE DE FRIBOURG CH (FRIBOURG) coordinator 187˙419.00

Map

 Project objective

Lipid droplets (LDs) are evolutionarily conserved dynamic organelles dedicated to storage of fat. Relatively little is known about the molecular processes that regulate nascent LD formation at specific sites in the endoplasmic reticulum (ER). Understanding LD biogenesis and degradation is crucial for deciphering the pathophysiology of LD storage disorders, like obesity, diabetes type-2, atherosclerosis, and lipodystrophy. The main objective of this project is to precisely map and characterize LD biogenesis sites in the ER using the model eukaryote, S. cerevisiae. One reason so little is known about the earliest stages of LD biogenesis is that until recently we have not had tools to visualize and characterize these sites. However, I have generated a yeast strain in which it is possible to use electron microscopy to image the earliest stages of LD biogenesis. In a recently published study I used this strain to characterize early stages of LD biogenesis and I propose to use this strain to investigate the role of a number of proteins in LD biogenesis by using mutants lacking these proteins. In addition, I have developed the first fluorescent protein marker of sites of nascent LD biogenesis. This new protein will allow me to use fluorescent microscopy to visualize LD biogenesis in live cells and in mutants lacking proteins known to be involved in LD biogenesis. I will also use this protein as a molecular tool to identify proteins and lipids that are enriched at LD biogenesis sites in the ER by performing immuno-purification and mass-spec analyses. The proposed studies will reveal and identify proteins and lipids necessary for the earliest stages of LD biogenesis and will make possible future mechanistic studies of LD biogenesis. My participation in “LD_biogenesis” will broaden my scientific expertise and hone my competences in becoming a successful project investigator.

 Publications

year authors and title journal last update
List of publications.
2020 Vineet Choudhary, Ola El Atab, Giulia Mizzon, William A. Prinz, Roger Schneiter
Seipin and Nem1 establish discrete ER subdomains to initiate yeast lipid droplet biogenesis.
published pages: , ISSN: 1540-8140, DOI: 10.1083/jcb.201910177.
Journal of Cell Biology 2020-04-15

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The information about "LD_BIOGENESIS" are provided by the European Opendata Portal: CORDIS opendata.

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