Opendata, web and dolomites


Mechanistic analysis of DNA damage signaling and bypass upon replication of damaged DNA template in human cells.

Total Cost €


EC-Contrib. €






 DNAcheck project word cloud

Explore the words cloud of the DNAcheck project. It provides you a very rough idea of what is the project "DNAcheck" about.

stability    arises    leads    damaged    networks    template    stalled    endogenous    global    replicative    generally    synthesis    gap    multidisciplinary    either    assumed    exo1    repair    helicase    accumulate    constitute    priming    forks    pcna    bypass    fundamental    machinery    mechanisms    replication    sense    templates    serious    genome    dna    remaining    light    actually    re    strand    genetic    single    association    movement    triggers    disease    interestingly    stranded    signal    activation    scenario    complete    function    checkpoint    left    employ    cells    sensed    restricted    encoded    originated    encounters    gaps    exogenous    human    stalling    humans    found    uncoupling    question    exonuclease    yeast    shed    lesions    prevent    ssdna    sources    breakage    downstream    site    seems    postreplicative    switching    unexplored    attack    constant    fork    clear    mechanism    molecular    polymerase    budding    poorly    predominant    conserved    damage    hence    signaling    extended    lagging   

Project "DNAcheck" data sheet

The following table provides information about the project.


Organization address
address: CALLE S. FERNANDO 4
postcode: 41004

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Spain [ES]
 Total cost 170˙121 €
 EC max contribution 170˙121 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2017
 Funding Scheme MSCA-IF-EF-ST
 Starting year 2018
 Duration (year-month-day) from 2018-10-01   to  2020-09-30


Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    UNIVERSIDAD DE SEVILLA ES (SEVILLA) coordinator 170˙121.00


 Project objective

The genetic information encoded by DNA is under constant attack from both endogenous and exogenous sources of damage. To ensure genome stability and prevent disease, cells use global signaling networks to sense and repair DNA damage. One particularly serious problem is when the replication machinery encounters lesions remaining in the template DNA. In this scenario, cells employ damage bypass mechanisms to complete genome replication and prevent fork breakage. Importantly, these pathways are not restricted to the site of stalling but can also function behind the fork at single-stranded DNA (ssDNA) gaps originated by the re-priming of DNA synthesis downstream of lesions. While it is very well known that ssDNA is the molecular signal that triggers the checkpoint response, it is less clear how and where ssDNA actually arises. Generally, it is assumed to accumulate at stalled replication forks, either by an uncoupling between replicative helicase and polymerase movement or between leading and lagging strand synthesis. However, in a recent study in budding yeast, I found that ssDNA gaps left behind replication forks, and extended by processing factors such as the exonuclease EXO1, constitute the predominant signal that leads to checkpoint activation in response to damaged DNA templates during S phase. Whether this mechanism of checkpoint activation is conserved from yeast to humans remains unexplored. Hence, using a unique set of multidisciplinary approaches, this project aims to address the fundamental question of how DNA damage is sensed during replication in human cells. Interestingly, not only ssDNA gap processing seems important for checkpoint signaling but also for the template switching mechanism of damage bypass. Therefore, this project will also study the function of EXO1 and its association with PCNA at postreplicative ssDNA gaps in order to shed light on the poorly understood mechanism of template switching.

Are you the coordinator (or a participant) of this project? Plaese send me more information about the "DNACHECK" project.

For instance: the website url (it has not provided by EU-opendata yet), the logo, a more detailed description of the project (in plain text as a rtf file or a word file), some pictures (as picture files, not embedded into any word file), twitter account, linkedin page, etc.

Send me an  email ( and I put them in your project's page as son as possible.

Thanks. And then put a link of this page into your project's website.

The information about "DNACHECK" are provided by the European Opendata Portal: CORDIS opendata.

More projects from the same programme (H2020-EU.1.3.2.)

MathematicsAnalogies (2019)

Mathematics Analogies

Read More  


The dark side of partial ownership and financial investment in Europe: What price to pay for consumers and society?

Read More  

MBL-Fermions (2020)

Probing many-body localization dynamics using ultracold fermions in an optical lattice

Read More