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THyM SIGNED

Why is Transparent Hypocotyl Mutant showed reduced phototropic response?

Total Cost €

0

EC-Contrib. €

0

Partnership

0

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 THyM project word cloud

Explore the words cloud of the THyM project. It provides you a very rough idea of what is the project "THyM" about.

fankhauser    auxin    wild    transparent    cell    understand    proteins    transported    gradients    initiated    insect    activated    stem    visualize    primary    cellular    phenotypic    functional    wall    pigment    regarding    fiber    microscopy    hypotheses    showed    drosophila    hypocotyls    vision    shares    receptors    plant    protein    homology    localization    transporter    characterization    photosynthesis    levels    stimulated    light    rapa    gfp    transporters    expression    gradient    mutant    orthologous    downstream    binding    insights    photosensory    organ    phototropin    biochemical    reorient    lab    blue    staining    asymmetric    environment    first    techniques    transport    plants    signaling    gene    play    abc    phototropism    family    microscopic    atp    hypocotyl    photo    subcellular    brasicca    tagged    optimize    conservation    components    precursor    atabc    establishment    mutants    eye    phenotype    favorable    substance    pattern    tissue    confocal    defective    function    perceiving    cassette    trigger    optic    vital    defect    underlies    testable   

Project "THyM" data sheet

The following table provides information about the project.

Coordinator
UNIVERSITE DE LAUSANNE 

Organization address
address: Quartier Unil-Centre Bâtiment Unicentre
city: LAUSANNE
postcode: 1015
website: www.unil.ch

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Switzerland [CH]
 Total cost 203˙149 €
 EC max contribution 203˙149 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2018
 Funding Scheme MSCA-IF-EF-ST
 Starting year 2020
 Duration (year-month-day) from 2020-03-01   to  2022-02-28

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    UNIVERSITE DE LAUSANNE CH (LAUSANNE) coordinator 203˙149.00

Map

 Project objective

Plants can reorient their growth towards a favorable light environment to optimize photosynthesis in a process called phototropism. This process is initiated by the phototropin blue light receptors, perceiving light gradients to trigger downstream signaling leading to the asymmetric distribution of auxin across the photo-stimulated stem. The Fankhauser lab showed that AtABC, an ABC (ATP-binding cassette) transporter family protein is important for phototropism. AtABC shares homology with Drosophila transporters, which are involved in eye pigment precursor transport and play a vital role in insect vision. In addition to reduced phototropism, Atabc mutants have transparent hypocotyls. The primary objective of this project is to understand the function of AtABC in phototropism and to test whether this transporter is required for light gradient establishment across the hypocotyl. First, I will characterize the Atabc mutant at the tissue, cellular and subcellular levels (e.g. staining for cell wall components) to determine what defect underlies the transparent hypocotyl phenotype. Using several approaches including confocal microscopy to visualize light-activated proteins and fiber-optic techniques, I will measure the light gradient across the hypocotyl of wild type and Atabc mutants. I will characterize phototropin signaling using biochemical and microscopic approaches to determine at which signaling step AtABC is required. To characterize AtABC, I will determine its expression pattern and subcellular localization using GFP-tagged AtABC. Together with the phenotypic characterization of the mutant, this will provide testable hypotheses regarding the substance(s) transported by AtABC. Finally, to determine the functional conservation of AtABC in other plants, I will characterize Brasicca rapa mutants defective in the orthologous gene. The functional characterization of AtABC may provide key insights into light gradient establishment in a plant photosensory organ.

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The information about "THYM" are provided by the European Opendata Portal: CORDIS opendata.

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