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THyM SIGNED

Why is Transparent Hypocotyl Mutant showed reduced phototropic response?

Total Cost €

0

EC-Contrib. €

0

Partnership

0

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 THyM project word cloud

Explore the words cloud of the THyM project. It provides you a very rough idea of what is the project "THyM" about.

techniques    vital    expression    function    light    biochemical    atp    initiated    transport    mutant    conservation    environment    hypocotyls    microscopic    plants    pigment    cell    trigger    cellular    transporters    showed    regarding    eye    organ    wall    wild    activated    reorient    transparent    plant    abc    components    levels    shares    mutants    gene    first    gradients    phototropism    phenotype    play    phototropin    insect    stimulated    insights    asymmetric    understand    gfp    precursor    underlies    stem    transported    photo    atabc    fankhauser    tagged    phenotypic    subcellular    pattern    defect    characterization    homology    optic    auxin    transporter    microscopy    blue    hypotheses    substance    cassette    perceiving    staining    photosensory    localization    establishment    family    photosynthesis    vision    downstream    favorable    defective    gradient    optimize    hypocotyl    rapa    confocal    receptors    testable    lab    primary    signaling    proteins    fiber    orthologous    brasicca    binding    tissue    drosophila    visualize    functional    protein   

Project "THyM" data sheet

The following table provides information about the project.

Coordinator
UNIVERSITE DE LAUSANNE 

Organization address
address: Quartier Unil-Centre Bâtiment Unicentre
city: LAUSANNE
postcode: 1015
website: www.unil.ch

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Switzerland [CH]
 Total cost 203˙149 €
 EC max contribution 203˙149 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2018
 Funding Scheme MSCA-IF-EF-ST
 Starting year 2020
 Duration (year-month-day) from 2020-03-01   to  2022-02-28

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    UNIVERSITE DE LAUSANNE CH (LAUSANNE) coordinator 203˙149.00

Map

 Project objective

Plants can reorient their growth towards a favorable light environment to optimize photosynthesis in a process called phototropism. This process is initiated by the phototropin blue light receptors, perceiving light gradients to trigger downstream signaling leading to the asymmetric distribution of auxin across the photo-stimulated stem. The Fankhauser lab showed that AtABC, an ABC (ATP-binding cassette) transporter family protein is important for phototropism. AtABC shares homology with Drosophila transporters, which are involved in eye pigment precursor transport and play a vital role in insect vision. In addition to reduced phototropism, Atabc mutants have transparent hypocotyls. The primary objective of this project is to understand the function of AtABC in phototropism and to test whether this transporter is required for light gradient establishment across the hypocotyl. First, I will characterize the Atabc mutant at the tissue, cellular and subcellular levels (e.g. staining for cell wall components) to determine what defect underlies the transparent hypocotyl phenotype. Using several approaches including confocal microscopy to visualize light-activated proteins and fiber-optic techniques, I will measure the light gradient across the hypocotyl of wild type and Atabc mutants. I will characterize phototropin signaling using biochemical and microscopic approaches to determine at which signaling step AtABC is required. To characterize AtABC, I will determine its expression pattern and subcellular localization using GFP-tagged AtABC. Together with the phenotypic characterization of the mutant, this will provide testable hypotheses regarding the substance(s) transported by AtABC. Finally, to determine the functional conservation of AtABC in other plants, I will characterize Brasicca rapa mutants defective in the orthologous gene. The functional characterization of AtABC may provide key insights into light gradient establishment in a plant photosensory organ.

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The information about "THYM" are provided by the European Opendata Portal: CORDIS opendata.

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