Opendata, web and dolomites

NanoProt-ID SIGNED

Proteome profiling using plasmonic nanopore sensors

Total Cost €

0

EC-Contrib. €

0

Partnership

0

Views

0

 NanoProt-ID project word cloud

Explore the words cloud of the NanoProt-ID project. It provides you a very rough idea of what is the project "NanoProt-ID" about.

learning    trace    necessitating    proteins    bioinformatics    regulating    classifier    equipped    lysine    biology    acids    ratio    resolution    amplifiers    gt    signal    breakthrough    first    individual    platform    vast    human    molecule    date    details    instead    time    one    99    reached    sense    cell    antibody    cells    opening    protein    rely    fingerprints    single    obtain    machine    respectively    cysteine    precision    averaging    nanopores    proteomic    custom    constitutes    vitro    technologies    devising    plasmonic    hypothesize    speed    solid    id    threading    fabrication    obscuring    appear    feasibility    proteomes    fluorophores    chain    amino    identification    residues    representing    methionine    proteomics    secreted    thousands    biological    transform    sensing    free    optical    uniquely    blood    color    fluorescence    cancer    attaining    directions    minutes    biomedicine    translocation    proteome    labelling    nanoprot    metastatic    noise    post   

Project "NanoProt-ID" data sheet

The following table provides information about the project.

Coordinator
TECHNION - ISRAEL INSTITUTE OF TECHNOLOGY 

Organization address
address: SENATE BUILDING TECHNION CITY
city: HAIFA
postcode: 32000
website: www.technion.ac.il

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Israel [IL]
 Total cost 2˙498˙869 €
 EC max contribution 2˙498˙869 € (100%)
 Programme 1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC))
 Code Call ERC-2018-ADG
 Funding Scheme ERC-ADG
 Starting year 2019
 Duration (year-month-day) from 2019-08-01   to  2024-07-31

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    TECHNION - ISRAEL INSTITUTE OF TECHNOLOGY IL (HAIFA) coordinator 2˙498˙869.00

Map

 Project objective

To date, antibody-free protein identification methods have not reached single-molecule precision. Instead, they rely on averaging from many cells, obscuring the details of important biological processes. The ability to identify each individual protein from within a single cell would transform proteomics research and biomedicine. However, single protein identification (ID) presents a major challenge, necessitating a breakthrough in single-molecule sensing technologies.

We propose to develop a method for proteome-level analysis, with single protein resolution. Bioinformatics studies show that >99% of human proteins can be uniquely identified by the order in which only three amino-acids, Lysine, Cysteine, and Methionine (K, C and M, respectively), appear along the proteins’ chain. By specifically labelling K, C and M residues with three distinct fluorophores, and threading them, one by one, through solid-state nanopores equipped with custom plasmonic amplifiers, we hypothesize that we can obtain multi-color fluorescence time-trace fingerprints uniquely representing most proteins in the human proteome. The feasibility of our method will be established by attaining 4 main aims: i) in vitro K,C,M protein labelling, ii) development of a machine learning classifier to uniquely ID proteins based on their optical fingerprints, iii) fabrication of state-of-the-art plasmonic nanopores for high-resolution optical sensing of proteins, and iv) devising methods for regulating the translocation speed to enhance the signal to noise ratio. Next, we will scale up our platform to enable the analysis of thousands of different proteins in minutes, and apply it to sense blood-secreted proteins, as well as whole proteomes in pre- and post-metastatic cancer cells. NanoProt-ID constitutes the first and most challenging step towards the proteomic analysis of individual cells, opening vast research directions and applications in biomedicine and systems biology.

Are you the coordinator (or a participant) of this project? Plaese send me more information about the "NANOPROT-ID" project.

For instance: the website url (it has not provided by EU-opendata yet), the logo, a more detailed description of the project (in plain text as a rtf file or a word file), some pictures (as picture files, not embedded into any word file), twitter account, linkedin page, etc.

Send me an  email (fabio@fabiodisconzi.com) and I put them in your project's page as son as possible.

Thanks. And then put a link of this page into your project's website.

The information about "NANOPROT-ID" are provided by the European Opendata Portal: CORDIS opendata.

More projects from the same programme (H2020-EU.1.1.)

CARBOFLOW (2020)

Streamlined carbon dioxide conversion in ionic liquids – a platform strategy for modern carbonylation chemistry

Read More  

UrbTerr (2019)

Urban Terrorism in Europe (2004-2019): Remembering, Imagining, and Anticipating Violence

Read More  

FLATBANDS (2020)

Exploring strong correlations in flat bands

Read More