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nanoCellSense SIGNED

A nanotechnology-based approach for label-free single-cell analysis of cytoplasmic proteome

Total Cost €

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EC-Contrib. €

0

Partnership

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 nanoCellSense project word cloud

Explore the words cloud of the nanoCellSense project. It provides you a very rough idea of what is the project "nanoCellSense" about.

ion    universal    assembled    opportune    tools    time    occurs    preservation    dispersed    extracellular    surface    nanopipette    chemically    binding    biology    desired    microscope    concentration    nanoprobe    aperture    determined    performing    inner    pathological    cellular    sharp    ionic    calibration    detection    label    fundamental    presently    possibilities    concentrations    sicm    monolayer    conductivity    occlusion    self    cell    cycle    intracellular    single    heterogeneity    purpose    environment    coated    framework    last    wall    advantage    pipette    fingerprint    cells    highlighting    molecular    insights    characterisation    came    first    stems    mainly    membrane    living    immobilisation    mechanisms    detected    perturbative    live    visualise    protein    coating    screening    scanning    functionalised    ultra    endowed    possibility    view    underlying    molecules    molecule    proteome    flowing    point    morphological    antibodies    free    relates    dynamics    hope    physiology    disease    mechanical    piercing    variation    followed    interaction    proteomic   

Project "nanoCellSense" data sheet

The following table provides information about the project.

Coordinator
IMPERIAL COLLEGE OF SCIENCE TECHNOLOGY AND MEDICINE 

Organization address
address: SOUTH KENSINGTON CAMPUS EXHIBITION ROAD
city: LONDON
postcode: SW7 2AZ
website: http://www.imperial.ac.uk/

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country United Kingdom [UK]
 Total cost 212˙933 €
 EC max contribution 212˙933 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2018
 Funding Scheme MSCA-IF-EF-ST
 Starting year 2019
 Duration (year-month-day) from 2019-12-01   to  2021-11-30

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    IMPERIAL COLLEGE OF SCIENCE TECHNOLOGY AND MEDICINE UK (LONDON) coordinator 212˙933.00

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 Project objective

Presently there is a strong need to single-cell tools able to provide new insights into cellular proteome heterogeneity underlying dynamics, mechanisms and cell states in development and disease. Of particular interest in this framework is the possibility to have an effective intra-cellular proteomic detection in live cells. This mainly for three reasons: the first relates to preservation of higher protein concentration if the detection occurs before a molecule is dispersed in the extracellular environment; the second reason stems from the need to study in greater detail and in real time, single cell processes such as the molecular cell cycle or the molecular fingerprint in a label-free way; the last reason came up from the universal necessity to know the intracellular concentrations of many molecules involved in pathological and not-pathological cellular processes. I hope to achieve the desired goal taking advantage of a scanning ion conductivity microscope (SICM) endowed with a chemically functionalised ultra-sharp nanopipette to visualise living cells highlighting, from the morphological and mechanical point of view, specific target cells, that would be further investigated in their molecular fingerprint piercing the cell membrane and performing a molecular immobilisation screening. For this purpose, the inner surface of the nanopipette will be coated with a self-assembled monolayer of antibodies to target the protein of interest. The interaction between the protein and the coating of the nanopipette would result in a variation in the ionic current flowing through the pipette due to aperture occlusion. Effective binding of the protein to the inner wall of the pipette will be detected and followed in real time and, after opportune nanoprobe calibration, intracellular concentration determined. This low-perturbative, label-free approach for single cell proteome characterisation will open to new possibilities for fundamental research in cell biology and physiology.

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