Opendata, web and dolomites

nanoCellSense SIGNED

A nanotechnology-based approach for label-free single-cell analysis of cytoplasmic proteome

Total Cost €

0

EC-Contrib. €

0

Partnership

0

Views

0

 nanoCellSense project word cloud

Explore the words cloud of the nanoCellSense project. It provides you a very rough idea of what is the project "nanoCellSense" about.

determined    underlying    label    intracellular    surface    morphological    functionalised    last    binding    single    presently    fingerprint    disease    mainly    cellular    molecules    screening    interaction    followed    proteomic    protein    perturbative    nanoprobe    coating    microscope    came    antibodies    self    sicm    relates    proteome    inner    cell    molecular    scanning    ion    molecule    dynamics    highlighting    environment    mechanical    detected    concentration    nanopipette    monolayer    cycle    calibration    endowed    extracellular    chemically    purpose    preservation    occlusion    ultra    conductivity    pipette    immobilisation    ionic    piercing    wall    tools    membrane    assembled    dispersed    sharp    framework    advantage    visualise    flowing    biology    detection    occurs    opportune    performing    fundamental    living    coated    live    physiology    cells    heterogeneity    desired    concentrations    hope    view    mechanisms    possibility    possibilities    characterisation    aperture    stems    time    variation    point    insights    free    pathological    universal    first   

Project "nanoCellSense" data sheet

The following table provides information about the project.

Coordinator
IMPERIAL COLLEGE OF SCIENCE TECHNOLOGY AND MEDICINE 

Organization address
address: SOUTH KENSINGTON CAMPUS EXHIBITION ROAD
city: LONDON
postcode: SW7 2AZ
website: http://www.imperial.ac.uk/

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country United Kingdom [UK]
 Total cost 212˙933 €
 EC max contribution 212˙933 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2018
 Funding Scheme MSCA-IF-EF-ST
 Starting year 2019
 Duration (year-month-day) from 2019-12-01   to  2021-11-30

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    IMPERIAL COLLEGE OF SCIENCE TECHNOLOGY AND MEDICINE UK (LONDON) coordinator 212˙933.00

Map

 Project objective

Presently there is a strong need to single-cell tools able to provide new insights into cellular proteome heterogeneity underlying dynamics, mechanisms and cell states in development and disease. Of particular interest in this framework is the possibility to have an effective intra-cellular proteomic detection in live cells. This mainly for three reasons: the first relates to preservation of higher protein concentration if the detection occurs before a molecule is dispersed in the extracellular environment; the second reason stems from the need to study in greater detail and in real time, single cell processes such as the molecular cell cycle or the molecular fingerprint in a label-free way; the last reason came up from the universal necessity to know the intracellular concentrations of many molecules involved in pathological and not-pathological cellular processes. I hope to achieve the desired goal taking advantage of a scanning ion conductivity microscope (SICM) endowed with a chemically functionalised ultra-sharp nanopipette to visualise living cells highlighting, from the morphological and mechanical point of view, specific target cells, that would be further investigated in their molecular fingerprint piercing the cell membrane and performing a molecular immobilisation screening. For this purpose, the inner surface of the nanopipette will be coated with a self-assembled monolayer of antibodies to target the protein of interest. The interaction between the protein and the coating of the nanopipette would result in a variation in the ionic current flowing through the pipette due to aperture occlusion. Effective binding of the protein to the inner wall of the pipette will be detected and followed in real time and, after opportune nanoprobe calibration, intracellular concentration determined. This low-perturbative, label-free approach for single cell proteome characterisation will open to new possibilities for fundamental research in cell biology and physiology.

Are you the coordinator (or a participant) of this project? Plaese send me more information about the "NANOCELLSENSE" project.

For instance: the website url (it has not provided by EU-opendata yet), the logo, a more detailed description of the project (in plain text as a rtf file or a word file), some pictures (as picture files, not embedded into any word file), twitter account, linkedin page, etc.

Send me an  email (fabio@fabiodisconzi.com) and I put them in your project's page as son as possible.

Thanks. And then put a link of this page into your project's website.

The information about "NANOCELLSENSE" are provided by the European Opendata Portal: CORDIS opendata.

More projects from the same programme (H2020-EU.1.3.2.)

Widow Spider Mating (2020)

Immature mating as a novel tactic of an invasive widow spider

Read More  

TARGET SLEEP (2020)

Boosting motor learning through sleep and targeted memory reactivation in ageing and Parkinson’s disease

Read More  

MY MITOCOMPLEX (2021)

Functional relevance of mitochondrial supercomplex assembly in myeloid cells

Read More