Opendata, web and dolomites

nanoCellSense SIGNED

A nanotechnology-based approach for label-free single-cell analysis of cytoplasmic proteome

Total Cost €

0

EC-Contrib. €

0

Partnership

0

Views

0

 nanoCellSense project word cloud

Explore the words cloud of the nanoCellSense project. It provides you a very rough idea of what is the project "nanoCellSense" about.

biology    cells    cycle    hope    morphological    perturbative    highlighting    immobilisation    proteome    detection    pipette    detected    coated    fundamental    time    ion    disease    aperture    cellular    inner    followed    mechanisms    opportune    performing    dispersed    protein    functionalised    possibility    live    presently    flowing    concentrations    heterogeneity    insights    assembled    molecule    intracellular    screening    piercing    characterisation    chemically    tools    free    underlying    binding    fingerprint    molecular    monolayer    view    antibodies    calibration    environment    sharp    ionic    label    mainly    sicm    possibilities    determined    occlusion    scanning    advantage    preservation    physiology    stems    surface    mechanical    purpose    last    single    occurs    endowed    ultra    cell    desired    relates    coating    pathological    interaction    dynamics    visualise    conductivity    point    microscope    concentration    first    proteomic    self    molecules    living    nanopipette    universal    membrane    framework    wall    came    nanoprobe    variation    extracellular   

Project "nanoCellSense" data sheet

The following table provides information about the project.

Coordinator
IMPERIAL COLLEGE OF SCIENCE TECHNOLOGY AND MEDICINE 

Organization address
address: SOUTH KENSINGTON CAMPUS EXHIBITION ROAD
city: LONDON
postcode: SW7 2AZ
website: http://www.imperial.ac.uk/

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country United Kingdom [UK]
 Total cost 212˙933 €
 EC max contribution 212˙933 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2018
 Funding Scheme MSCA-IF-EF-ST
 Starting year 2019
 Duration (year-month-day) from 2019-12-01   to  2021-11-30

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    IMPERIAL COLLEGE OF SCIENCE TECHNOLOGY AND MEDICINE UK (LONDON) coordinator 212˙933.00

Map

 Project objective

Presently there is a strong need to single-cell tools able to provide new insights into cellular proteome heterogeneity underlying dynamics, mechanisms and cell states in development and disease. Of particular interest in this framework is the possibility to have an effective intra-cellular proteomic detection in live cells. This mainly for three reasons: the first relates to preservation of higher protein concentration if the detection occurs before a molecule is dispersed in the extracellular environment; the second reason stems from the need to study in greater detail and in real time, single cell processes such as the molecular cell cycle or the molecular fingerprint in a label-free way; the last reason came up from the universal necessity to know the intracellular concentrations of many molecules involved in pathological and not-pathological cellular processes. I hope to achieve the desired goal taking advantage of a scanning ion conductivity microscope (SICM) endowed with a chemically functionalised ultra-sharp nanopipette to visualise living cells highlighting, from the morphological and mechanical point of view, specific target cells, that would be further investigated in their molecular fingerprint piercing the cell membrane and performing a molecular immobilisation screening. For this purpose, the inner surface of the nanopipette will be coated with a self-assembled monolayer of antibodies to target the protein of interest. The interaction between the protein and the coating of the nanopipette would result in a variation in the ionic current flowing through the pipette due to aperture occlusion. Effective binding of the protein to the inner wall of the pipette will be detected and followed in real time and, after opportune nanoprobe calibration, intracellular concentration determined. This low-perturbative, label-free approach for single cell proteome characterisation will open to new possibilities for fundamental research in cell biology and physiology.

Are you the coordinator (or a participant) of this project? Plaese send me more information about the "NANOCELLSENSE" project.

For instance: the website url (it has not provided by EU-opendata yet), the logo, a more detailed description of the project (in plain text as a rtf file or a word file), some pictures (as picture files, not embedded into any word file), twitter account, linkedin page, etc.

Send me an  email (fabio@fabiodisconzi.com) and I put them in your project's page as son as possible.

Thanks. And then put a link of this page into your project's website.

The information about "NANOCELLSENSE" are provided by the European Opendata Portal: CORDIS opendata.

More projects from the same programme (H2020-EU.1.3.2.)

MIGPSC (2018)

Shaping the European Migration Policy: the role of the security industry

Read More  

DiMaS (2019)

Retrospective genomic analyses of shortfin Mako shark (Isurus oxyrinchus) using DNA from archived jaws

Read More  

PNAIC (2018)

Positive and Negative Asymmetry in Intergroup Contact: Its Impact on Linguistic Forms of Communication and Physiological Responses

Read More