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InScope SIGNED

Integrated analysis of regulatory networks modulating seed coat permeability in natural accessions

Total Cost €

0

EC-Contrib. €

0

Partnership

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 InScope project word cloud

Explore the words cloud of the InScope project. It provides you a very rough idea of what is the project "InScope" about.

tolerance    haplotype    sk    marie    controls    meeting    phenotypes    cks    quality    modified    wild    basis    isolated    longevity    expression    examined    arr    coat    odowska    fellowship    generate    edited    networks    spatio    regulator    modulate    embryo    candidate    demand    shorter    cytological    network    wp1    permeability    transcriptional    industrial    mechanisms    regulatory    structural    natural    seed    expansion    coats    layers    accessions    fundamental    promoter    hormone    arr16    appropriate    cytokinins    reporter    laser    transcriptomes    tissues    signaling    genome    cas9    lines    plant    arabidopsis    cis    reveal    precisely    outcomes    performed    constant    skills    surround    association    wp2    copies    market    stress    temporal    specialized    seeds    individual    composition    defects    constituted    mutants    permeable    curie    innovative    area    crispr    dormancy    ascertain    gfp    cells    thaliana    microdissection    lower    notably    feasible    mutant    cell    mediated    germination    gene    wp3   

Project "InScope" data sheet

The following table provides information about the project.

Coordinator
INSTITUT NATIONAL DE RECHERCHE POUR L'AGRICULTURE, L'ALIMENTATION ET L'ENVIRONNEMENT 

Organization address
address: Rue De L'Universite 147
city: PARIS CEDEX 07
postcode: 75338
website: www.inra.fr

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country France [FR]
 Total cost 196˙707 €
 EC max contribution 196˙707 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2018
 Funding Scheme MSCA-IF-EF-ST
 Starting year 2019
 Duration (year-month-day) from 2019-11-01   to  2021-10-31

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    INSTITUT NATIONAL DE RECHERCHE POUR L'AGRICULTURE, L'ALIMENTATION ET L'ENVIRONNEMENT FR (PARIS CEDEX 07) coordinator 196˙707.00

Map

 Project objective

The European seed market is in constant expansion and improving seed quality is a key factor towards meeting the increase in demand. The seed coat is constituted from multi-cell layers that surround the embryo that will become the future plant. Seed coat properties and composition have a significant impact on seed quality. Notably, mutant seeds with permeable seed coats have defects in germination phenotypes such as lower dormancy, shorter longevity and reduced stress tolerance. The regulatory mechanisms that modulate seed coat permeability are, however, not well understood. The candidate has performed a genome-wide association study in Arabidopsis thaliana and identified ARR16 as a novel regulatory gene affecting permeability, this Arabidopsis thaliana response regulator (ARR) is a signaling component for the plant hormone, cytokinins (CKs). The project objective is to determine the transcriptional network involving ARR16 that controls permeability in natural accessions. In work package 1 (WP1), the spatio-temporal expression of ARR16 will be examined in seed tissues of permeable and non-permeable accessions with GFP reporter lines. Also, we will generate mutants with CRISPR/Cas9 gene edited haplotype copies, aiming to precisely define the cis-elements in the ARR16 promoter that determine seed permeability. Next, cytological analyses will be carried out on seed coats of ARR16 mutants to ascertain the composition and structural basis of the modified permeability (WP2). Finally, transcriptomes of mutant and wild-type seed coat cells isolated by laser microdissection will be analyzed, aiming to reveal CKs signaling networks mediated by ARR16 in seeds (WP3). The proposed project is feasible and appropriate for a Marie Skłodowska-Curie Individual Fellowship since the expected outcomes are innovative and generate knowledge for both fundamental and industrial research, while the candidate will improve his skills and knowledge of a specialized research area.

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The information about "INSCOPE" are provided by the European Opendata Portal: CORDIS opendata.

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