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InScope SIGNED

Integrated analysis of regulatory networks modulating seed coat permeability in natural accessions

Total Cost €

0

EC-Contrib. €

0

Partnership

0

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 InScope project word cloud

Explore the words cloud of the InScope project. It provides you a very rough idea of what is the project "InScope" about.

cell    crispr    area    haplotype    coat    spatio    shorter    quality    innovative    wp3    composition    embryo    laser    gfp    cytokinins    basis    permeable    copies    coats    meeting    reveal    defects    fellowship    candidate    networks    modified    structural    cas9    plant    demand    germination    stress    promoter    ascertain    genome    reporter    mutant    seeds    precisely    outcomes    arr16    cis    longevity    surround    phenotypes    tolerance    hormone    seed    natural    association    lower    microdissection    accessions    lines    constituted    isolated    odowska    individual    skills    constant    modulate    performed    cytological    curie    signaling    regulatory    industrial    expression    regulator    gene    permeability    wild    layers    notably    mutants    wp2    sk    edited    controls    mediated    arr    arabidopsis    cells    market    feasible    dormancy    transcriptomes    expansion    network    tissues    marie    temporal    appropriate    specialized    fundamental    generate    examined    transcriptional    thaliana    wp1    cks    mechanisms   

Project "InScope" data sheet

The following table provides information about the project.

Coordinator
INSTITUT NATIONAL DE RECHERCHE POUR L'AGRICULTURE, L'ALIMENTATION ET L'ENVIRONNEMENT 

Organization address
address: Rue De L'Universite 147
city: PARIS CEDEX 07
postcode: 75338
website: www.inra.fr

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country France [FR]
 Total cost 196˙707 €
 EC max contribution 196˙707 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2018
 Funding Scheme MSCA-IF-EF-ST
 Starting year 2019
 Duration (year-month-day) from 2019-11-01   to  2021-10-31

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    INSTITUT NATIONAL DE RECHERCHE POUR L'AGRICULTURE, L'ALIMENTATION ET L'ENVIRONNEMENT FR (PARIS CEDEX 07) coordinator 196˙707.00

Map

 Project objective

The European seed market is in constant expansion and improving seed quality is a key factor towards meeting the increase in demand. The seed coat is constituted from multi-cell layers that surround the embryo that will become the future plant. Seed coat properties and composition have a significant impact on seed quality. Notably, mutant seeds with permeable seed coats have defects in germination phenotypes such as lower dormancy, shorter longevity and reduced stress tolerance. The regulatory mechanisms that modulate seed coat permeability are, however, not well understood. The candidate has performed a genome-wide association study in Arabidopsis thaliana and identified ARR16 as a novel regulatory gene affecting permeability, this Arabidopsis thaliana response regulator (ARR) is a signaling component for the plant hormone, cytokinins (CKs). The project objective is to determine the transcriptional network involving ARR16 that controls permeability in natural accessions. In work package 1 (WP1), the spatio-temporal expression of ARR16 will be examined in seed tissues of permeable and non-permeable accessions with GFP reporter lines. Also, we will generate mutants with CRISPR/Cas9 gene edited haplotype copies, aiming to precisely define the cis-elements in the ARR16 promoter that determine seed permeability. Next, cytological analyses will be carried out on seed coats of ARR16 mutants to ascertain the composition and structural basis of the modified permeability (WP2). Finally, transcriptomes of mutant and wild-type seed coat cells isolated by laser microdissection will be analyzed, aiming to reveal CKs signaling networks mediated by ARR16 in seeds (WP3). The proposed project is feasible and appropriate for a Marie Skłodowska-Curie Individual Fellowship since the expected outcomes are innovative and generate knowledge for both fundamental and industrial research, while the candidate will improve his skills and knowledge of a specialized research area.

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The information about "INSCOPE" are provided by the European Opendata Portal: CORDIS opendata.

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