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InScope SIGNED

Integrated analysis of regulatory networks modulating seed coat permeability in natural accessions

Total Cost €

0

EC-Contrib. €

0

Partnership

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 InScope project word cloud

Explore the words cloud of the InScope project. It provides you a very rough idea of what is the project "InScope" about.

ascertain    embryo    defects    examined    candidate    gfp    meeting    haplotype    longevity    layers    structural    wild    reveal    permeable    skills    marie    germination    mediated    arr16    cks    coat    regulatory    constant    accessions    area    shorter    reporter    odowska    plant    market    outcomes    mechanisms    transcriptomes    wp1    cell    modified    controls    regulator    wp3    copies    curie    seeds    precisely    cells    mutants    surround    cytokinins    lower    microdissection    expansion    coats    innovative    thaliana    demand    gene    stress    expression    appropriate    cis    quality    arr    transcriptional    networks    edited    association    generate    dormancy    mutant    fundamental    crispr    permeability    spatio    cas9    tissues    fellowship    feasible    constituted    performed    isolated    specialized    natural    notably    promoter    modulate    phenotypes    lines    seed    basis    industrial    genome    signaling    laser    wp2    individual    sk    cytological    arabidopsis    network    hormone    composition    tolerance    temporal   

Project "InScope" data sheet

The following table provides information about the project.

Coordinator
INSTITUT NATIONAL DE RECHERCHE POUR L'AGRICULTURE, L'ALIMENTATION ET L'ENVIRONNEMENT 

Organization address
address: Rue De L'Universite 147
city: PARIS CEDEX 07
postcode: 75338
website: www.inra.fr

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country France [FR]
 Total cost 196˙707 €
 EC max contribution 196˙707 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2018
 Funding Scheme MSCA-IF-EF-ST
 Starting year 2019
 Duration (year-month-day) from 2019-11-01   to  2021-10-31

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    INSTITUT NATIONAL DE RECHERCHE POUR L'AGRICULTURE, L'ALIMENTATION ET L'ENVIRONNEMENT FR (PARIS CEDEX 07) coordinator 196˙707.00

Map

 Project objective

The European seed market is in constant expansion and improving seed quality is a key factor towards meeting the increase in demand. The seed coat is constituted from multi-cell layers that surround the embryo that will become the future plant. Seed coat properties and composition have a significant impact on seed quality. Notably, mutant seeds with permeable seed coats have defects in germination phenotypes such as lower dormancy, shorter longevity and reduced stress tolerance. The regulatory mechanisms that modulate seed coat permeability are, however, not well understood. The candidate has performed a genome-wide association study in Arabidopsis thaliana and identified ARR16 as a novel regulatory gene affecting permeability, this Arabidopsis thaliana response regulator (ARR) is a signaling component for the plant hormone, cytokinins (CKs). The project objective is to determine the transcriptional network involving ARR16 that controls permeability in natural accessions. In work package 1 (WP1), the spatio-temporal expression of ARR16 will be examined in seed tissues of permeable and non-permeable accessions with GFP reporter lines. Also, we will generate mutants with CRISPR/Cas9 gene edited haplotype copies, aiming to precisely define the cis-elements in the ARR16 promoter that determine seed permeability. Next, cytological analyses will be carried out on seed coats of ARR16 mutants to ascertain the composition and structural basis of the modified permeability (WP2). Finally, transcriptomes of mutant and wild-type seed coat cells isolated by laser microdissection will be analyzed, aiming to reveal CKs signaling networks mediated by ARR16 in seeds (WP3). The proposed project is feasible and appropriate for a Marie Skłodowska-Curie Individual Fellowship since the expected outcomes are innovative and generate knowledge for both fundamental and industrial research, while the candidate will improve his skills and knowledge of a specialized research area.

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The information about "INSCOPE" are provided by the European Opendata Portal: CORDIS opendata.

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