METACODE

Code-engineered new-to-nature microbial cell factories for novel and safety enhanced bio-production

 Coordinatore TECHNISCHE UNIVERSITAT BERLIN 

 Organization address address: STRASSE DES 17 JUNI 135
city: BERLIN
postcode: 10623

contact info
Titolo: Ms.
Nome: Silke
Cognome: Hönert
Email: send email
Telefono: +49 30 314 79973
Fax: +49 30 314 21689

 Nazionalità Coordinatore Germany [DE]
 Totale costo 3˙892˙149 €
 EC contributo 2˙996˙938 €
 Programma FP7-KBBE
Specific Programme "Cooperation": Food, Agriculture and Biotechnology
 Code Call FP7-KBBE-2011-5
 Funding Scheme CP-FP
 Anno di inizio 2011
 Periodo (anno-mese-giorno) 2011-12-01   -   2015-11-30

 Partecipanti

# participant  country  role  EC contrib. [€] 
1    TECHNISCHE UNIVERSITAT BERLIN

 Organization address address: STRASSE DES 17 JUNI 135
city: BERLIN
postcode: 10623

contact info
Titolo: Ms.
Nome: Silke
Cognome: Hönert
Email: send email
Telefono: +49 30 314 79973
Fax: +49 30 314 21689

DE (BERLIN) coordinator 898˙809.00
2    EIDGENOESSISCHE TECHNISCHE HOCHSCHULE ZURICH

 Organization address address: Raemistrasse 101
city: ZUERICH
postcode: 8092

contact info
Titolo: Prof.
Nome: Sven
Cognome: Panke
Email: send email
Telefono: +41 61 3873209
Fax: +41 61 3873994

CH (ZUERICH) participant 499˙237.00
3    Isthmus

 Organization address address: Rue Saint Amand 31
city: Paris
postcode: 75015

contact info
Titolo: Mr.
Nome: Pierre
Cognome: Dedenys
Email: send email
Telefono: +33 160 874 585
Fax: +33 160 874586

FR (Paris) participant 343˙992.00
4    BIOFACTION KG

 Organization address address: KUNDMANNGASSE 39/12
city: WIEN
postcode: 1030

contact info
Titolo: Dr.
Nome: Markus
Cognome: Schmidt
Email: send email
Telefono: 436607000000

AT (WIEN) participant 264˙320.00
5    KATHOLIEKE UNIVERSITEIT LEUVEN

 Organization address address: Oude Markt 13
city: LEUVEN
postcode: 3000

contact info
Titolo: Ms.
Nome: Sarah
Cognome: Malevé
Email: send email
Telefono: +32 16 320611
Fax: +32 16 326515

BE (LEUVEN) participant 250˙000.00
6    COMMISSARIAT A L ENERGIE ATOMIQUE ET AUX ENERGIES ALTERNATIVES

 Organization address address: RUE LEBLANC 25
city: PARIS 15
postcode: 75015

contact info
Titolo: Ms.
Nome: Mathilde
Cognome: Suret
Email: send email
Telefono: +33 159 082 256

FR (PARIS 15) participant 249˙780.00
7    UNIVERSITAET BASEL

 Organization address address: Petersplatz 1
city: BASEL
postcode: 4003

contact info
Titolo: Prof.
Nome: Thomas
Cognome: Ward
Email: send email
Telefono: +41 61 2671004
Fax: +41 61 2671005

CH (BASEL) participant 249˙600.00
8    Molecular Networks GmbH - Computerchemie

 Organization address address: Henkestrasse 91
city: Erlangen
postcode: 91052

contact info
Titolo: Prof.
Nome: Johann
Cognome: Gasteiger
Email: send email
Telefono: 499132000000
Fax: 499132000000

DE (Erlangen) participant 241˙200.00

Mappa


 Word cloud

Esplora la "nuvola delle parole (Word Cloud) per avere un'idea di massima del progetto.

evolution    scientists    peptide    give    enzyme    mass    codons    bacteria    strains    chemistries    industrial    acids    living    engineering    organisms    natural    parallel    genetic    metacode    amino    metathesis    protein    manner    canonical    engineered    synthetic    desired    reactions    re    functionalities    firewall    orthogonal    perform    bio    alternative    artificial    life    biotechnology    sense    microbial    chemical    code    clones    directed   

 Obiettivo del progetto (Objective)

'The concept of METACODE is to preform genetic code engineering in microbial strains with parallel recruitment of novel bio-orthogonal chemistries for mass production of desired protein/peptide based products. In combination with computational and classical chemical synthetic approaches as well as chemo-informatics, enzyme guided evolution, synthetic metabolism, and directed evolution of microbial strains, artificial industrial microbial strains will be designed. This will enable the access to genetically robust and safe strains with added/novel functionalities and topologies from renewable resources. These strains will be characterized with alternative reading of the genetic code (genetic firewall) and with predetermined chemistries (metathesis), as well as necessary robustness for efficient industrial use.'

Introduzione (Teaser)

Enhancing the chemical repertoire of life would lead to novel molecules with parameters that could be adapted for various fermentative production processes. European scientists have realised this scenario by integrating artificial amino acids into the cellular machinery of microorganisms to augment bio-synthetic pathways with non-natural chemistries.

Descrizione progetto (Article)

Despite the complexity of living organisms, their chemical composition is relatively simple and is organised on a surprisingly limited set of chemistries.

If we were able to interfere with the chemistry of life, we would essentially be able to make synthetic mechanisms that carry desirable chemical functionalities and reactions.

Scientists of the EU-funded 'Code-engineered new-to-nature microbial cell factories for novel and safety enhanced bio-production' (http://www.meta-code.eu/ (METACODE)) project believe that the most promising route to achieve this is to modify existing organisms to include amino acid building blocks beyond the canonical 20. Scientists plan to perform genetic code engineering in microbial strains in parallel with novel bio-orthogonal chemistries.

This would lead to mass production of desired protein/peptide-based products with new chemical functionalities and give new dimensions to existing biocatalysis.

METACODE-generated engineered bacterial clones constitute a genetic firewall. As these clones read the genetic code in an alternative manner to natural bacteria, they will be unable to horizontally transfer their genetic material to natural bacteria. Additionally, they will be able to perform enzyme-catalysed metathesis reactions that do not exist in living organisms. This unique property will be exploited in biotechnology applications, for example, for generating artificial metalloenzymes and novel peptide antibiotics.

To incorporate non-canonical amino acids into growing polypeptide chains, the consortium had to design a strategy for re-assigning the genetic code to these amino acids. To this end, they set up an automated system to perform directed evolution of Escherichia coli strains to free low-usage sense codons and non-sense codons and re-assign them to non-canonical amino acids. This 'genetic code emancipation' would expand ribosomal peptide/protein biogenesis and give rise to novel peptides with broad biological, chemical and physical properties.

The METACODE project proved the feasibility of directed evolution of microbial strains in a controlled manner. This represents a fundamental scientific breakthrough in the field and has extraordinary potential for drug development and biotechnology.

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