Explore the words cloud of the ChromArch project. It provides you a very rough idea of what is the project "ChromArch" about.
The following table provides information about the project.
|Coordinator Country||Germany [DE]|
|Total cost||1˙486˙578 €|
|EC max contribution||1˙486˙578 € (100%)|
1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC))
|Duration (year-month-day)||from 2015-05-01 to 2021-04-30|
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Chromatin packaging into the nucleus of eukaryotic cells is highly sophisticated. It not only serves to condense the genomic content into restricted space, but mainly to encode epigenetic traits ensuring temporally controlled and balanced transcription of genes and coordinated DNA replication and repair. The non-random three-dimensional chromatin architecture including looped structures between genomic control elements relies on the action of architectural proteins. However, despite increasing interest in spatio-temporal chromatin organization, mechanistic details of their contributions are not well understood.
With this proposal I aim at unveiling molecular mechanisms of protein–mediated chromatin organization by in vivo single molecule tracking and quantitative super-resolution imaging of architectural proteins using reflected light sheet microscopy (RLSM). I will measure the interaction dynamics, the spatial distribution and the stoichiometry of architectural proteins throughout the nucleus and at specific chromatin loci within single cells. In complement single molecule force spectroscopy experiments using magnetic tweezers (MT), I will study mechanisms of DNA loop formation in vitro by structure-mediating proteins.
Integrating these spatio-temporal and mechanical single molecule information, I will in the third sup-project measure the dynamics of relative end-to-end movements and the forces acting within a looped chromatin structure in living cells.
Taken together, my experiments will greatly enhance our mechanistic understanding of three-dimensional chromatin architecture and inspire future experiments on its regulatory effects on nuclear functions and potential therapeutic utility upon controlled modification.
|year||authors and title||journal||last update|
A. GroÃŸe-Berkenbusch, J. Hettich, T. Kuhn, N. Fili, A.W. Cook, Y. Hari-Gupta, A. Palmer, L. Streit, P.J.I. Ellis, C.P. Toseland* and J.C.M. Gebhardt
Myosin VI moves on nuclear actin filaments and supports long-range chromatin rearrangements
published pages: , ISSN: , DOI: 10.1101/2020.04.03.023614
Johannes Hettich, J. Christof M. Gebhardt
Computer aided interactive gene network simulations including stochastic molecular kinetics and noise
published pages: , ISSN: , DOI: 10.1101/872374
Karen ClauÃŸ, Achim P. Popp, Lena Schulze, Johannes Hettich, Matthias Reisser, Laura EscoterÂ Torres, N. Henriette Uhlenhaut, J.Â ChristofÂ M. Gebhardt
DNA residence time is a regulatory factor of transcription repression
published pages: 11121-11130, ISSN: 0305-1048, DOI: 10.1093/nar/gkx728
|Nucleic Acids Research 45/19||2019-09-02|
Matthias Reisser, Anja Palmer, Achim P. Popp, Christopher Jahn, Gilbert Weidinger, J. Christof M. Gebhardt
Single-molecule imaging correlates decreasing nuclear volume with increasing TF-chromatin associations during zebrafish development
published pages: , ISSN: 2041-1723, DOI: 10.1038/s41467-018-07731-8
|Nature Communications 9/1||2019-09-02|
Harsha Agarwal, Matthias Reisser, Celina Wortmann, J. Christof M. Gebhardt
Direct Observation of Cell-Cycle-Dependent Interactions between CTCF and Chromatin
published pages: 2051-2055, ISSN: 0006-3495, DOI: 10.1016/j.bpj.2017.04.018
|Biophysical Journal 112/10||2019-09-02|
J. Hettich, J.C.M. Gebhardt
Transcription factor target site search and gene regulation in a background of unspecific binding sites
published pages: , ISSN: 0022-5193, DOI: 10.1016/j.jtbi.2018.05.037
|Journal of Theoretical Biology||2019-09-02|
Rahel Stefanie Wiehe, Boris Gole, Laurent Chatre, Paul Walther, Enrico Calzia, Anja Palmer, J. Christof M. Gebhardt, Miria Ricchetti, Lisa WiesmÃ¼ller
Correction: Endonuclease G promotes mitochondrial genome cleavage and replication
published pages: , ISSN: 1949-2553, DOI: 10.18632/oncotarget.25645
MahÃ© Raccaud, Elias T. Friman, Andrea B. Alber, Harsha Agarwal, CÃ©dric Deluz, Timo Kuhn, J. Christof M. Gebhardt, David M. Suter
Mitotic chromosome binding predicts transcription factor properties in interphase
published pages: , ISSN: 2041-1723, DOI: 10.1038/s41467-019-08417-5
|Nature Communications 10/1||2019-09-02|
Lisa Hipp, Judith Beer, Oliver Kuchler, Matthias Reisser, Daniela Sinske, Jens Michaelis, J. Christof M. Gebhardt, Bernd KnÃ¶ll
Single-molecule imaging of the transcription factor SRF reveals prolonged chromatin-binding kinetics upon cell stimulation
published pages: 201812734, ISSN: 0027-8424, DOI: 10.1073/pnas.1812734116
|Proceedings of the National Academy of Sciences||2019-09-02|
Matthias Reisser, Johannes Hettich, Timo Kuhn, J. Christof M. Gebhardt
Inferring quantity and qualities of superimposed reaction rates in single molecule survival time distributions
published pages: , ISSN: , DOI: 10.1101/679258
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The information about "CHROMARCH" are provided by the European Opendata Portal: CORDIS opendata.
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