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ACTOMYO SIGNED

Mechanisms of actomyosin-based contractility during cytokinesis

Total Cost €

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EC-Contrib. €

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Partnership

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 ACTOMYO project word cloud

Explore the words cloud of the ACTOMYO project. It provides you a very rough idea of what is the project "ACTOMYO" about.

organism    assembled    significantly    electron    repair    live    accomplished    remarkably    image    microcopy    ultra    poorly    completes    interdisciplinary    signaling    mechanism    cellular    perpendicularly    ring    constriction    function    microsurgery    accurate    kinetics    expert    structural    assembly    division    remodeled    cytokinesis    fluorescently    plane    mother    branched    facilitates    fundamental    nematode    experimentally    filament    uncovered    power    contrast    full    suited    dividing    contractile    profiling    actomyosin    molecular    dynamics    elegans    components    embryo    cells    metazoan    screen    powerful    thickness    dynamically    functional    uniquely    phenotype    tagged    cell    replacement    networks    contribution    mechanisms    assays    rnai    overwhelmingly    levels    partitioning    contractility    assay    quantitative    itself    questions    view    structure    organization    laser    explore    network    width    involve    populations    world    contents    mechanistic    combining    dictate    actin    tractable    daughter    imaging   

Project "ACTOMYO" data sheet

The following table provides information about the project.

Coordinator
INSTITUTO DE BIOLOGIA MOLECULAR E CELULAR-IBMC 

Organization address
address: RUA ALFREDO ALLEN 208
city: PORTO
postcode: 4200 135
website: www.ibmc.up.pt

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Portugal [PT]
 Project website https://www.i3s.up.pt/research-group
 Total cost 1˙499˙988 €
 EC max contribution 1˙499˙988 € (100%)
 Programme 1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC))
 Code Call ERC-2014-STG
 Funding Scheme ERC-STG
 Starting year 2015
 Duration (year-month-day) from 2015-07-01   to  2021-06-30

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    INSTITUTO DE BIOLOGIA MOLECULAR E CELULAR-IBMC PT (PORTO) coordinator 1˙499˙988.00

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 Project objective

Cytokinesis completes cell division by partitioning the contents of the mother cell to the two daughter cells. This process is accomplished through the assembly and constriction of a contractile ring, a complex actomyosin network that remains poorly understood on the molecular level. Research in cytokinesis has overwhelmingly focused on signaling mechanisms that dictate when and where the contractile ring is assembled. By contrast, the research I propose here addresses fundamental questions about the structural and functional properties of the contractile ring itself. We will use the nematode C. elegans to exploit the power of quantitative live imaging assays in an experimentally tractable metazoan organism. The early C. elegans embryo is uniquely suited to the study of the contractile ring, as cells dividing perpendicularly to the imaging plane provide a full end-on view of the contractile ring throughout constriction. This greatly facilitates accurate measurements of constriction kinetics, ring width and thickness, and levels as well as dynamics of fluorescently-tagged contractile ring components. Combining image-based assays with powerful molecular replacement technology for structure-function studies, we will 1) determine the contribution of branched and non-branched actin filament populations to contractile ring formation; 2) explore its ultra-structural organization in collaboration with a world expert in electron microcopy; 3) investigate how the contractile ring network is dynamically remodeled during constriction with the help of a novel laser microsurgery assay that has uncovered a remarkably robust ring repair mechanism; and 4) use a targeted RNAi screen and phenotype profiling to identify new components of actomyosin contractile networks. The results from this interdisciplinary project will significantly enhance our mechanistic understanding of cytokinesis and other cellular processes that involve actomyosin-based contractility.

 Publications

year authors and title journal last update
List of publications.
2018 Cláudia Pereira, Rita M. Reis, José B. Gama, Ricardo Celestino, Dhanya K. Cheerambathur, Ana X. Carvalho, Reto Gassmann
Self-Assembly of the RZZ Complex into Filaments Drives Kinetochore Expansion in the Absence of Microtubule Attachment
published pages: 3408-3421.e8, ISSN: 0960-9822, DOI: 10.1016/j.cub.2018.08.056
Current Biology 28/21 2020-04-01
2019 Ricardo Celestino, Morkos A. Henen, José B. Gama, Cátia Carvalho, Maxwell McCabe, Daniel J. Barbosa, Alexandra Born, Parker J. Nichols, Ana X. Carvalho, Reto Gassmann, Beat Vögeli
A transient helix in the disordered region of dynein light intermediate chain links the motor to structurally diverse adaptors for cargo transport
published pages: e3000100, ISSN: 1545-7885, DOI: 10.1371/journal.pbio.3000100
PLOS Biology 17/1 2020-04-01
2019 Fung-Yi Chan, Ana M. Silva, Joana Saramago, Joana Pereira-Sousa, Hailey E. Brighton, Marisa Pereira, Karen Oegema, Reto Gassmann, Ana Xavier Carvalho
The ARP2/3 complex prevents excessive formin activity during cytokinesis
published pages: 96-107, ISSN: 1059-1524, DOI: 10.1091/mbc.e18-07-0471
Molecular Biology of the Cell 30/1 2020-04-01
2017 José B. Gama, Cláudia Pereira, Patrícia A. Simões, Ricardo Celestino, Rita M. Reis, Daniel J. Barbosa, Helena R. Pires, Cátia Carvalho, João Amorim, Ana X. Carvalho, Dhanya K. Cheerambathur, Reto Gassmann
Molecular mechanism of dynein recruitment to kinetochores by the Rod–Zw10–Zwilch complex and Spindly
published pages: 943-960, ISSN: 0021-9525, DOI: 10.1083/jcb.201610108
The Journal of Cell Biology 216/4 2020-04-01
2018 Patrícia A. Simões, Ricardo Celestino, Ana X. Carvalho, Reto Gassmann
NudE regulates dynein at kinetochores but is dispensable for other dynein functions in the C. elegans early embryo
published pages: jcs212159, ISSN: 0021-9533, DOI: 10.1242/jcs.212159
Journal of Cell Science 131/1 2020-04-01
2017 C.Thieleke-Matos, D.S.Osório, A.X.Carvalho, E.Morais-de-Sá
Emerging Mechanisms and Roles for Asymmetric Cytokinesis
published pages: , ISSN: , DOI:
International Review of Cell and Molecular Biology 2020-04-01
2018 Neide Vieira, Carlos Bessa, Ana J. Rodrigues, Paulo Marques, Fung-Yi Chan, Ana Xavier de Carvalho, Margarida Correia-Neves, Nuno Sousa
Sorting nexin 3 mutation impairs development and neuronal function in Caenorhabditis elegans
published pages: 2027-2044, ISSN: 1420-682X, DOI: 10.1007/s00018-017-2719-2
Cellular and Molecular Life Sciences 75/11 2020-04-01
2016 Silva AM, Osório DS, Pereira AJ, Maiato H, Pinto IM, Rubinstein B, Gassmann R, Telley IA, Carvalho AX.
Robust gap repair in the contractile ring ensures timely completion of cytokinesis.
published pages: , ISSN: 0021-9525, DOI: 10.1083/jcb.201605080
JOURNAL OF CELL BIOLOGY 2020-04-01
2017 Daniel J. Barbosa, Joana Duro, Bram Prevo, Dhanya K. Cheerambathur, Ana X. Carvalho, Reto Gassmann
Dynactin binding to tyrosinated microtubules promotes centrosome centration in C. elegans by enhancing dynein-mediated organelle transport
published pages: e1006941, ISSN: 1553-7404, DOI: 10.1371/journal.pgen.1006941
PLOS Genetics 13/7 2020-04-01
2018 Sriyash Mangal, Jennifer Sacher, Taekyung Kim, Daniel Sampaio Osório, Fumio Motegi, Ana Xavier Carvalho, Karen Oegema, Esther Zanin
TPXL-1 activates Aurora A to clear contractile ring components from the polar cortex during cytokinesis
published pages: 837-848, ISSN: 0021-9525, DOI: 10.1083/jcb.201706021
The Journal of Cell Biology 217/3 2020-04-01

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