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MolMap SIGNED

From Tissues to Single Molecules: High Content in Situ Super-Resolution imaging with DNA-PAINT

Total Cost €

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EC-Contrib. €

0

Partnership

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 MolMap project word cloud

Explore the words cloud of the MolMap project. It provides you a very rough idea of what is the project "MolMap" about.

envelope    exchange    network    relevance    localize    characterization    automated    techniques    unraveling    platform    once    pressing    lattice    paint    differentiation    clinical    labeling    question    dna    issue    protein    proteins    combination    localization    cascades    biological    molecular    advent    fluorescence    interplay    cells    mass    interactions    resolution    acquisition    progression    signaling    tools    microscopy    immense    single    highest    super    acids    tissue    transformative    deep    exploring    powerful    networks    limited    cell    technically    microscope    answer    multiplexing    either    technological    components    image    multiplexed    quantify    sheet    mutual    understand    health    impedes    disease    cellular    stimuli    disruptive    quantitative    microenvironment    basis    tool    biomolecules    nanotechnology    push    nanobody    capability    barcoding    revolutionize    spatial    nucleic    molecule    abundance    throughput    tissues    transcriptomics    aptamer    spectrometry    despite    first    acid    light    proteomics    imaging    multitude    positively    lack   

Project "MolMap" data sheet

The following table provides information about the project.

Coordinator
LUDWIG-MAXIMILIANS-UNIVERSITAET MUENCHEN 

Organization address
address: GESCHWISTER SCHOLL PLATZ 1
city: MUENCHEN
postcode: 80539
website: www.uni-muenchen.de

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Germany [DE]
 Total cost 1˙695˙000 €
 EC max contribution 1˙695˙000 € (100%)
 Programme 1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC))
 Code Call ERC-2015-STG
 Funding Scheme ERC-STG
 Starting year 2016
 Duration (year-month-day) from 2016-04-01   to  2021-03-31

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    LUDWIG-MAXIMILIANS-UNIVERSITAET MUENCHEN DE (MUENCHEN) coordinator 1˙695˙000.00

Map

 Project objective

Fluorescence microscopy is a powerful tool for exploring biomolecules in cells and tissues, especially with the advent of super-resolution techniques. To better understand key processes such as cell differentiation and disease progression, it is crucial to investigate the abundance, localization and mutual interactions of crucial cellular components such as nucleic acids and proteins. Unraveling their complex interplay in whole signaling networks is necessary to investigate cellular responses to stimuli. However, currently available characterization techniques are either limited by low multiplexing capability (e.g. fluorescence microscopy) or lack localization information (e.g. mass spectrometry). Despite the immense biological and clinical relevance of understanding network-wide changes, the lack of a technological platform to image, identify and quantify a multitude of key protein networks at high spatial resolution in tissues impedes our understanding of the molecular basis of health and disease. I aim to solve this pressing issue and revolutionize fluorescence microscopy using tools from DNA Nanotechnology with transformative potential to positively answer the question: Can we localize and identify each protein or nucleic acid molecule in a complex tissue microenvironment? The approach is based on my recently developed DNA- and Exchange-PAINT techniques. To push the envelope of what’s technically possible I will first build a lattice light-sheet microscope for deep tissue high throughput DNA-PAINT imaging. Second, I will develop novel nanobody- and aptamer-based labeling approaches in combination with molecular barcoding and automated multiplexed image acquisition and processing. With these disruptive and transformative tools, I will investigate whole signaling cascades at once in single cells and whole tissues, thus enabling quantitative imaging transcriptomics and proteomics with highest spatial resolution.

 Publications

year authors and title journal last update
List of publications.
2017 Sarit S. Agasti, Yu Wang, Florian Schueder, Aishwarya Sukumar, Ralf Jungmann, Peng Yin
DNA-barcoded labeling probes for highly multiplexed Exchange-PAINT imaging
published pages: 3080-3091, ISSN: 2041-6520, DOI: 10.1039/c6sc05420j
Chemical Science 8/4 2019-06-19
2018 Maximilian T. Strauss, Florian Schueder, Daniel Haas, Philipp C. Nickels, Ralf Jungmann
Quantifying absolute addressability in DNA origami with molecular resolution
published pages: , ISSN: 2041-1723, DOI: 10.1038/s41467-018-04031-z
Nature Communications 9/1 2019-06-19
2017 Florian Schueder, Maximilian T. Strauss, David Hoerl, Joerg Schnitzbauer, Thomas Schlichthaerle, Sebastian Strauss, Peng Yin, Hartmann Harz, Heinrich Leonhardt, Ralf Jungmann
Universal Super-Resolution Multiplexing by DNA Exchange
published pages: 4052-4055, ISSN: 1433-7851, DOI: 10.1002/anie.201611729
Angewandte Chemie International Edition 56/14 2019-06-19
2018 Thomas Schlichthaerle, Alexandra S. Eklund, Florian Schueder, Maximilian T. Strauss, Christian Tiede, Alistair Curd, Jonas Ries, Michelle Peckham, Darren C. Tomlinson, Ralf Jungmann
Site-Specific Labeling of Affimers for DNA-PAINT Microscopy
published pages: 11060-11063, ISSN: 1433-7851, DOI: 10.1002/anie.201804020
Angewandte Chemie International Edition 57/34 2019-04-18
2018 Sebastian Strauss, Philipp C. Nickels, Maximilian T. Strauss, Vilma Jimenez Sabinina, Jan Ellenberg, Jeffrey D. Carter, Shashi Gupta, Nebojsa Janjic, Ralf Jungmann
Modified aptamers enable quantitative sub-10-nm cellular DNA-PAINT imaging
published pages: 685-688, ISSN: 1548-7091, DOI: 10.1038/s41592-018-0105-0
Nature Methods 15/9 2019-04-18
2017 Alexander Auer, Maximilian T. Strauss, Thomas Schlichthaerle, Ralf Jungmann
Fast, Background-Free DNA-PAINT Imaging Using FRET-Based Probes
published pages: 6428-6434, ISSN: 1530-6984, DOI: 10.1021/acs.nanolett.7b03425
Nano Letters 17/10 2019-04-18
2017 Johannes B. Woehrstein, Maximilian T. Strauss, Luvena L. Ong, Bryan Wei, David Y. Zhang, Ralf Jungmann, Peng Yin
Sub–100-nm metafluorophores with digitally tunable optical properties self-assembled from DNA
published pages: e1602128, ISSN: 2375-2548, DOI: 10.1126/sciadv.1602128
Science Advances 3/6 2019-04-18
2018 Jonas Mücksch, Philipp Blumhardt, Maximilian T. Strauss, Eugene P. Petrov, Ralf Jungmann, Petra Schwille
Quantifying Reversible Surface Binding via Surface-Integrated Fluorescence Correlation Spectroscopy
published pages: 3185-3192, ISSN: 1530-6984, DOI: 10.1021/acs.nanolett.8b00875
Nano Letters 18/5 2019-04-18
2017 Florian Schueder, Juanita Lara-Gutiérrez, Brian J. Beliveau, Sinem K. Saka, Hiroshi M. Sasaki, Johannes B. Woehrstein, Maximilian T. Strauss, Heinrich Grabmayr, Peng Yin, Ralf Jungmann
Multiplexed 3D super-resolution imaging of whole cells using spinning disk confocal microscopy and DNA-PAINT
published pages: , ISSN: 2041-1723, DOI: 10.1038/s41467-017-02028-8
Nature Communications 8/1 2019-04-18

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