SynthHotSpot SIGNED
Synthesizing Meiotic Crossover Hotspots in Arabidopsis
Total Cost €
0EC-Contrib. €
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Project "SynthHotSpot" data sheet
The following table provides information about the project.
| Coordinator |
THE CHANCELLOR MASTERS AND SCHOLARSOF THE UNIVERSITY OF CAMBRIDGE
Organization address contact info |
| Coordinator Country | United Kingdom [UK] |
| Project website | https://www.plantsci.cam.ac.uk/directory/henderson-ian |
| Total cost | 1˙999˙953 € |
| EC max contribution | 1˙999˙953 € (100%) |
| Programme |
1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC)) |
| Code Call | ERC-2015-CoG |
| Funding Scheme | ERC-COG |
| Starting year | 2016 |
| Duration (year-month-day) | from 2016-10-01 to 2021-09-30 |
Partnership
Take a look of project's partnership.
| # | ||||
|---|---|---|---|---|
| 1 | THE CHANCELLOR MASTERS AND SCHOLARSOF THE UNIVERSITY OF CAMBRIDGE | UK (CAMBRIDGE) | coordinator | 1˙999˙953.00 |
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Project objective
The majority of eukaryotes reproduce sexually via meiosis. During meiosis homologous chromosomes pair and undergo reciprocal genetic exchange termed crossover. Meiotic recombination is a major evolutionary force and has a profound effect on patterns of genetic diversity in sexual species. Crossovers distributions are highly non-random and are typically focused in narrow hotspots. Study of hotspots throughout eukaryotes has revealed combinations of genetic and epigenetic factors that contribute to their distributions. In this proposal we will use the extensive genetics and genomics tools available in Arabidopsis to comprehensively dissect hotspot patterning. The strategic aim of the proposal is to use this knowledge to direct de novo hotspots to loci of choice. In the first aim we will use functional genomics to profile the chromosomal distributions of key recombination proteins and test the role of chromatin and higher-order structures in driving these patterns. In the second aim we will study individual hotspots at the fine-scale, to the resolution of individual polymorphisms, using amplification and sequencing of recombinant molecules from gamete DNA. To test genetic versus epigenetic control of hotspots we will use genome-editing to delete hotspot-associated CTT-repeat DNA sequence motifs, in addition to directing DNA methylation in order to epigenetically silence recombination. In the final aim we will use our combined knowledge of hotspot control to implement genome-editing technologies (TALENs & CRISPR-Cas9) during meiosis. This will allow us to rationally control hotspot locations, which will be definitive proof that our models for recombination control are correct. This technology will also accelerate breeding and genome-engineering of our most important crops, where recombination can be limiting. Finally, mapping hotspots will allow us to better understand patterns of natural genetic diversity, including detecting the signatures of selection.
Publications
| year | authors and title | journal | last update |
|---|---|---|---|
| 2018 |
Heïdi Serra, Kyuha Choi, Xiaohui Zhao, Alexander R. Blackwell, Juhyun Kim, Ian R. Henderson Interhomolog polymorphism shapes meiotic crossover within the Arabidopsis RAC1 and RPP13 disease resistance genes published pages: e1007843, ISSN: 1553-7404, DOI: 10.1371/journal.pgen.1007843 |
PLOS Genetics 14/12 | 2020-01-22 |
| 2018 |
Kyuha Choi, Xiaohui Zhao, Andrew J. Tock, Christophe Lambing, Charles J. Underwood, Thomas J. Hardcastle, Heïdi Serra, Juhyun Kim, Hyun Seob Cho, Jaeil Kim, Piotr A. Ziolkowski, Nataliya E. Yelina, Ildoo Hwang, Robert A. Martienssen, Ian R. Henderson Nucleosomes and DNA methylation shape meiotic DSB frequency in Arabidopsis thaliana transposons and gene regulatory regions published pages: 532-546, ISSN: 1088-9051, DOI: 10.1101/gr.225599.117 |
Genome Research 28/4 | 2020-01-22 |
| 2018 |
Charles J. Underwood, Kyuha Choi, Christophe Lambing, Xiaohui Zhao, Heïdi Serra, Filipe Borges, Joe Simorowski, Evan Ernst, Yannick Jacob, Ian R. Henderson, Robert A. Martienssen Epigenetic activation of meiotic recombination near Arabidopsis thaliana centromeres via loss of H3K9me2 and non-CG DNA methylation published pages: 519-531, ISSN: 1088-9051, DOI: 10.1101/gr.227116.117 |
Genome Research 28/4 | 2020-01-22 |
| 2018 |
Heïdi Serra, Christophe Lambing, Catherine H. Griffin, Stephanie D. Topp, Divyashree C. Nageswaran, Charles J. Underwood, Piotr A. Ziolkowski, Mathilde Séguéla-Arnaud, Joiselle B. Fernandes, Raphaël Mercier, Ian R. Henderson Massive crossover elevation via combination of HEI10 and recq4a recq4b during Arabidopsis meiosis published pages: 2437-2442, ISSN: 0027-8424, DOI: 10.1073/pnas.1713071115 |
Proceedings of the National Academy of Sciences 115/10 | 2020-01-22 |
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