Opendata, web and dolomites

T-Rex SIGNED

Clathrin-mediated endocytosis in plants: mechanistic insight into the TPLATE REcycling compleX and its interplay with AP-2

Total Cost €

0

EC-Contrib. €

0

Partnership

0

Views

0

 T-Rex project word cloud

Explore the words cloud of the T-Rex project. It provides you a very rough idea of what is the project "T-Rex" about.

questions    mediated    recognition    avoidance    overlapping    world    toxin    proteomics    mechanistically    structural    modulate    anchored    cme    endocytic    answer    dynamic    recruitment    deep    live    adjustment    abundance    plants    additional    complexes    evolutionary    subunit    cargo    shown    wp3    ap    interactions    mechanistic    combine    spatio    model    biology    ways    wp1    unravel    wp4    hormone    removing    acute    nutrient    developmentally    functions    clathrin    endocytosis    wp5    independent    removal    adaptor    protein    domain    biological    imaging    group    couple    mainly    temporal    plant    cell    driving    proteome    gain    conserved    occurs    specifically    plasma    recognize    defence    functionality    tpc    proteins    cells    color    causal    wall    pm    constantly    newly    internalization    level    subunits    retention    dependent    poorly    structures    contrast    integrative    membrane    hereto    wp2    communicate    effectors    adjust    tplate    me    implying    dynamics    outside   

Project "T-Rex" data sheet

The following table provides information about the project.

Coordinator
VIB VZW 

Organization address
address: RIJVISSCHESTRAAT 120
city: ZWIJNAARDE - GENT
postcode: 9052
website: www.vib.be

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Belgium [BE]
 Total cost 1˙998˙813 €
 EC max contribution 1˙998˙813 € (100%)
 Programme 1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC))
 Code Call ERC-2015-CoG
 Funding Scheme ERC-COG
 Starting year 2016
 Duration (year-month-day) from 2016-07-01   to  2021-06-30

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    VIB VZW BE (ZWIJNAARDE - GENT) coordinator 1˙998˙813.00

Map

 Project objective

Cells communicate with the outside world through proteins anchored in their plasma membrane and hereto constantly adjust their plasma membrane (PM) proteome. In this adjustment process, removing proteins from the PM mainly occurs through clathrin-mediated endocytosis (CME). Mechanistically however, this process remains poorly understood in plants.

A recent study from my group has shown that, in contrast to other model systems, plant CME involves two early endocytic adaptor protein complexes: the evolutionary conserved Adaptor Protein 2 complex (AP-2) and the newly identified TPLATE complex (TPC). In the same study, we also show that both complexes have overlapping but also independent functions in driving CME in plants, implying that plants use additional ways to recognize membrane proteins (cargo) for internalization. In this project I will use an integrative approach to unravel the early steps of CME in plants. Specifically, I will address the following biological questions:

- Is the evolutionary retention of the TPC in plants causal to specific cargo recognition? (WP1) - What are the spatio-temporal dynamics of TPC and CME effectors at the plasma membrane? (WP2) - How does acute removal of TPC subunits affect complex recruitment and CME? (WP3) - How is the TPC organized at the structural level? (WP4) - Which interactions occur and can we couple subunit/domain structures to functionality? (WP5)

To answer these questions, I will combine state-of-the art proteomics with highly dynamic multi-color live cell imaging and structural biology.

The overall objective is to gain a deep mechanistic insight into the developmentally essential process of CME in plants. This will enable me to specifically specifically modulate the abundance of plasma membrane proteins involved in nutrient uptake, toxin avoidance, cell wall formation and hormone and defence responses. Understanding TPC-dependent CME will also provide insight into evolutionary aspects of endocytosis.

 Publications

year authors and title journal last update
List of publications.
2019 Deepanksha Arora, Nikolaj B. Abel, Chen Liu, Petra Van Damme, Lam Dai Vu, Anna Tornkvist, Francis Impens, Dominique Eeckhout, Alain Goossens, Geert De Jaeger, Thomas Ott, Panagiotis Moschou, Daniel Van Damme
Establishment of Proximity-dependent Biotinylation Approaches in Different Plant Model Systems
published pages: , ISSN: , DOI: 10.1101/701425
BioRxiv 2020-01-28
2018 Xingyun Qi, Roman Pleskot, Niloufer G. Irani, Daniël Van Damme
Meeting report – Cellular gateways: expanding the role of endocytosis in plant development
published pages: jcs222604, ISSN: 0021-9533, DOI: 10.1242/jcs.222604
Journal of Cell Science 131/17 2020-01-28

Are you the coordinator (or a participant) of this project? Plaese send me more information about the "T-REX" project.

For instance: the website url (it has not provided by EU-opendata yet), the logo, a more detailed description of the project (in plain text as a rtf file or a word file), some pictures (as picture files, not embedded into any word file), twitter account, linkedin page, etc.

Send me an  email (fabio@fabiodisconzi.com) and I put them in your project's page as son as possible.

Thanks. And then put a link of this page into your project's website.

The information about "T-REX" are provided by the European Opendata Portal: CORDIS opendata.

More projects from the same programme (H2020-EU.1.1.)

inhibiTOR (2020)

Novel selective mTORC1 inhibitors

Read More  

DOUBLE-TROUBLE (2020)

Replaying the ‘genome duplication’ tape of life: the importance of polyploidy for adaptation in a changing environment

Read More  

ARCTIC (2020)

Air Transport as Information and Computation

Read More