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T-Rex SIGNED

Clathrin-mediated endocytosis in plants: mechanistic insight into the TPLATE REcycling compleX and its interplay with AP-2

Total Cost €

0

EC-Contrib. €

0

Partnership

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 T-Rex project word cloud

Explore the words cloud of the T-Rex project. It provides you a very rough idea of what is the project "T-Rex" about.

cell    ap    tplate    combine    mediated    endocytosis    driving    questions    modulate    outside    gain    unravel    wp2    functions    biological    couple    anchored    removal    defence    removing    biology    acute    wp1    model    adjust    temporal    wall    answer    additional    structural    protein    ways    wp4    membrane    level    shown    imaging    recruitment    toxin    spatio    wp5    deep    group    recognition    complexes    cargo    functionality    proteins    proteome    clathrin    causal    proteomics    newly    communicate    nutrient    plant    retention    independent    dynamics    evolutionary    poorly    conserved    hereto    developmentally    effectors    recognize    specifically    overlapping    pm    wp3    subunits    abundance    contrast    domain    avoidance    color    adaptor    hormone    structures    plants    dynamic    dependent    interactions    plasma    constantly    me    mainly    integrative    cme    cells    live    subunit    mechanistic    world    adjustment    endocytic    tpc    mechanistically    implying    occurs    internalization   

Project "T-Rex" data sheet

The following table provides information about the project.

Coordinator
VIB VZW 

Organization address
address: RIJVISSCHESTRAAT 120
city: ZWIJNAARDE - GENT
postcode: 9052
website: www.vib.be

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Belgium [BE]
 Total cost 1˙998˙813 €
 EC max contribution 1˙998˙813 € (100%)
 Programme 1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC))
 Code Call ERC-2015-CoG
 Funding Scheme ERC-COG
 Starting year 2016
 Duration (year-month-day) from 2016-07-01   to  2021-06-30

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    VIB VZW BE (ZWIJNAARDE - GENT) coordinator 1˙998˙813.00

Map

 Project objective

Cells communicate with the outside world through proteins anchored in their plasma membrane and hereto constantly adjust their plasma membrane (PM) proteome. In this adjustment process, removing proteins from the PM mainly occurs through clathrin-mediated endocytosis (CME). Mechanistically however, this process remains poorly understood in plants.

A recent study from my group has shown that, in contrast to other model systems, plant CME involves two early endocytic adaptor protein complexes: the evolutionary conserved Adaptor Protein 2 complex (AP-2) and the newly identified TPLATE complex (TPC). In the same study, we also show that both complexes have overlapping but also independent functions in driving CME in plants, implying that plants use additional ways to recognize membrane proteins (cargo) for internalization. In this project I will use an integrative approach to unravel the early steps of CME in plants. Specifically, I will address the following biological questions:

- Is the evolutionary retention of the TPC in plants causal to specific cargo recognition? (WP1) - What are the spatio-temporal dynamics of TPC and CME effectors at the plasma membrane? (WP2) - How does acute removal of TPC subunits affect complex recruitment and CME? (WP3) - How is the TPC organized at the structural level? (WP4) - Which interactions occur and can we couple subunit/domain structures to functionality? (WP5)

To answer these questions, I will combine state-of-the art proteomics with highly dynamic multi-color live cell imaging and structural biology.

The overall objective is to gain a deep mechanistic insight into the developmentally essential process of CME in plants. This will enable me to specifically specifically modulate the abundance of plasma membrane proteins involved in nutrient uptake, toxin avoidance, cell wall formation and hormone and defence responses. Understanding TPC-dependent CME will also provide insight into evolutionary aspects of endocytosis.

 Publications

year authors and title journal last update
List of publications.
2019 Deepanksha Arora, Nikolaj B. Abel, Chen Liu, Petra Van Damme, Lam Dai Vu, Anna Tornkvist, Francis Impens, Dominique Eeckhout, Alain Goossens, Geert De Jaeger, Thomas Ott, Panagiotis Moschou, Daniel Van Damme
Establishment of Proximity-dependent Biotinylation Approaches in Different Plant Model Systems
published pages: , ISSN: , DOI: 10.1101/701425
BioRxiv 2020-01-28
2018 Xingyun Qi, Roman Pleskot, Niloufer G. Irani, Daniël Van Damme
Meeting report – Cellular gateways: expanding the role of endocytosis in plant development
published pages: jcs222604, ISSN: 0021-9533, DOI: 10.1242/jcs.222604
Journal of Cell Science 131/17 2020-01-28

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