Opendata, web and dolomites

T-Rex SIGNED

Clathrin-mediated endocytosis in plants: mechanistic insight into the TPLATE REcycling compleX and its interplay with AP-2

Total Cost €

0

EC-Contrib. €

0

Partnership

0

Views

0

 T-Rex project word cloud

Explore the words cloud of the T-Rex project. It provides you a very rough idea of what is the project "T-Rex" about.

contrast    tplate    live    temporal    defence    dynamics    independent    mediated    recognition    biology    plants    mainly    ap    model    removing    spatio    group    newly    mechanistic    combine    constantly    outside    additional    structures    clathrin    color    conserved    proteins    wp3    evolutionary    toxin    causal    recruitment    cells    functionality    nutrient    integrative    cargo    wp4    dynamic    removal    proteome    endocytosis    dependent    interactions    overlapping    communicate    pm    retention    ways    implying    poorly    answer    driving    world    structural    subunit    wp2    protein    wp1    proteomics    wp5    adjustment    tpc    couple    plasma    endocytic    adaptor    domain    developmentally    gain    mechanistically    plant    occurs    membrane    modulate    internalization    me    subunits    level    cell    wall    functions    hereto    effectors    abundance    anchored    recognize    biological    adjust    shown    specifically    deep    avoidance    hormone    complexes    acute    questions    imaging    unravel    cme   

Project "T-Rex" data sheet

The following table provides information about the project.

Coordinator
VIB VZW 

Organization address
address: RIJVISSCHESTRAAT 120
city: ZWIJNAARDE - GENT
postcode: 9052
website: www.vib.be

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Belgium [BE]
 Total cost 1˙998˙813 €
 EC max contribution 1˙998˙813 € (100%)
 Programme 1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC))
 Code Call ERC-2015-CoG
 Funding Scheme ERC-COG
 Starting year 2016
 Duration (year-month-day) from 2016-07-01   to  2021-06-30

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    VIB VZW BE (ZWIJNAARDE - GENT) coordinator 1˙998˙813.00

Map

 Project objective

Cells communicate with the outside world through proteins anchored in their plasma membrane and hereto constantly adjust their plasma membrane (PM) proteome. In this adjustment process, removing proteins from the PM mainly occurs through clathrin-mediated endocytosis (CME). Mechanistically however, this process remains poorly understood in plants.

A recent study from my group has shown that, in contrast to other model systems, plant CME involves two early endocytic adaptor protein complexes: the evolutionary conserved Adaptor Protein 2 complex (AP-2) and the newly identified TPLATE complex (TPC). In the same study, we also show that both complexes have overlapping but also independent functions in driving CME in plants, implying that plants use additional ways to recognize membrane proteins (cargo) for internalization. In this project I will use an integrative approach to unravel the early steps of CME in plants. Specifically, I will address the following biological questions:

- Is the evolutionary retention of the TPC in plants causal to specific cargo recognition? (WP1) - What are the spatio-temporal dynamics of TPC and CME effectors at the plasma membrane? (WP2) - How does acute removal of TPC subunits affect complex recruitment and CME? (WP3) - How is the TPC organized at the structural level? (WP4) - Which interactions occur and can we couple subunit/domain structures to functionality? (WP5)

To answer these questions, I will combine state-of-the art proteomics with highly dynamic multi-color live cell imaging and structural biology.

The overall objective is to gain a deep mechanistic insight into the developmentally essential process of CME in plants. This will enable me to specifically specifically modulate the abundance of plasma membrane proteins involved in nutrient uptake, toxin avoidance, cell wall formation and hormone and defence responses. Understanding TPC-dependent CME will also provide insight into evolutionary aspects of endocytosis.

 Publications

year authors and title journal last update
List of publications.
2019 Deepanksha Arora, Nikolaj B. Abel, Chen Liu, Petra Van Damme, Lam Dai Vu, Anna Tornkvist, Francis Impens, Dominique Eeckhout, Alain Goossens, Geert De Jaeger, Thomas Ott, Panagiotis Moschou, Daniel Van Damme
Establishment of Proximity-dependent Biotinylation Approaches in Different Plant Model Systems
published pages: , ISSN: , DOI: 10.1101/701425
BioRxiv 2020-01-28
2018 Xingyun Qi, Roman Pleskot, Niloufer G. Irani, Daniël Van Damme
Meeting report – Cellular gateways: expanding the role of endocytosis in plant development
published pages: jcs222604, ISSN: 0021-9533, DOI: 10.1242/jcs.222604
Journal of Cell Science 131/17 2020-01-28

Are you the coordinator (or a participant) of this project? Plaese send me more information about the "T-REX" project.

For instance: the website url (it has not provided by EU-opendata yet), the logo, a more detailed description of the project (in plain text as a rtf file or a word file), some pictures (as picture files, not embedded into any word file), twitter account, linkedin page, etc.

Send me an  email (fabio@fabiodisconzi.com) and I put them in your project's page as son as possible.

Thanks. And then put a link of this page into your project's website.

The information about "T-REX" are provided by the European Opendata Portal: CORDIS opendata.

More projects from the same programme (H2020-EU.1.1.)

ENUF (2019)

Evaluation of Novel Ultra-Fast selective III-V Epitaxy

Read More  

PonD (2019)

Particles-on-Demand for Multiscale Fluid Dynamics

Read More  

Diverge (2019)

Generation of ultra-deep libraries of transcriptional activators for gene therapy

Read More