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T-Rex SIGNED

Clathrin-mediated endocytosis in plants: mechanistic insight into the TPLATE REcycling compleX and its interplay with AP-2

Total Cost €

EC-Contrib. €

Partnership

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T-Rex project word cloud

Explore the words cloud of the T-Rex project. It provides you a very rough idea of what is the project "T-Rex" about.

gain proteomics wp3 endocytosis poorly cell spatio wp2 biological recognize ways integrative answer model wp5 evolutionary communicate mainly temporal specifically overlapping imaging wp1 endocytic developmentally cells independent domain dynamics level deep newly combine structural proteins wp4 driving clathrin structures interactions dynamic internalization complexes avoidance nutrient subunits causal additional wall outside conserved mechanistic proteome color mediated hormone shown retention couple questions removal plant effectors functionality recognition removing membrane adaptor live biology cme plasma functions group me protein ap hereto adjustment acute recruitment modulate toxin pm mechanistically tplate anchored implying constantly defence plants unravel subunit occurs adjust contrast dependent world cargo tpc abundance

Project "T-Rex" data sheet

The following table provides information about the project.

Coordinator	VIB VZW Organization address address: RIJVISSCHESTRAAT 120 city: ZWIJNAARDE - GENT postcode: 9052 website: www.vib.be contact info title: n.a. name: n.a. surname: n.a. function: n.a. email: n.a. telephone: n.a. fax: n.a.
Coordinator Country	Belgium [BE]
Total cost	1˙998˙813 €
EC max contribution	1˙998˙813 € (100%)
Programme	1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC))
Code Call	ERC-2015-CoG
Funding Scheme	ERC-COG
Starting year	2016
Duration (year-month-day)	from 2016-07-01 to 2021-06-30

Partnership

Take a look of project's partnership.

#	participants	country	role	EC contrib. [€]
1	VIB VZW VIB VZW Organization address address: RIJVISSCHESTRAAT 120 city: ZWIJNAARDE - GENT postcode: 9052 website: www.vib.be contact info title: n.a. name: n.a. surname: n.a. function: n.a. email: n.a. telephone: n.a. fax: n.a.	BE (ZWIJNAARDE - GENT)	coordinator	1˙998˙813.00

Map

Project objective

Cells communicate with the outside world through proteins anchored in their plasma membrane and hereto constantly adjust their plasma membrane (PM) proteome. In this adjustment process, removing proteins from the PM mainly occurs through clathrin-mediated endocytosis (CME). Mechanistically however, this process remains poorly understood in plants.

A recent study from my group has shown that, in contrast to other model systems, plant CME involves two early endocytic adaptor protein complexes: the evolutionary conserved Adaptor Protein 2 complex (AP-2) and the newly identified TPLATE complex (TPC). In the same study, we also show that both complexes have overlapping but also independent functions in driving CME in plants, implying that plants use additional ways to recognize membrane proteins (cargo) for internalization. In this project I will use an integrative approach to unravel the early steps of CME in plants. Specifically, I will address the following biological questions:

- Is the evolutionary retention of the TPC in plants causal to specific cargo recognition? (WP1) - What are the spatio-temporal dynamics of TPC and CME effectors at the plasma membrane? (WP2) - How does acute removal of TPC subunits affect complex recruitment and CME? (WP3) - How is the TPC organized at the structural level? (WP4) - Which interactions occur and can we couple subunit/domain structures to functionality? (WP5)

To answer these questions, I will combine state-of-the art proteomics with highly dynamic multi-color live cell imaging and structural biology.

The overall objective is to gain a deep mechanistic insight into the developmentally essential process of CME in plants. This will enable me to specifically specifically modulate the abundance of plasma membrane proteins involved in nutrient uptake, toxin avoidance, cell wall formation and hormone and defence responses. Understanding TPC-dependent CME will also provide insight into evolutionary aspects of endocytosis.

Publications

List of publications.
year	authors and title	journal	last update
2019	Deepanksha Arora, Nikolaj B. Abel, Chen Liu, Petra Van Damme, Lam Dai Vu, Anna Tornkvist, Francis Impens, Dominique Eeckhout, Alain Goossens, Geert De Jaeger, Thomas Ott, Panagiotis Moschou, Daniel Van Damme Establishment of Proximity-dependent Biotinylation Approaches in Different Plant Model Systems published pages: , ISSN: , DOI: 10.1101/701425	BioRxiv	2020-01-28
2018	Xingyun Qi, Roman Pleskot, Niloufer G. Irani, DaniÃ«l Van Damme Meeting report â€“ Cellular gateways: expanding the role of endocytosis in plant development published pages: jcs222604, ISSN: 0021-9533, DOI: 10.1242/jcs.222604	Journal of Cell Science 131/17	2020-01-28

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