Opendata, web and dolomites

ATG9_SOLVES_IT SIGNED

In vitro high resolution reconstitution of autophagosome nucleation and expansion catalyzed byATG9

Total Cost €

0

EC-Contrib. €

0

Partnership

0

Views

0

 ATG9_SOLVES_IT project word cloud

Explore the words cloud of the ATG9_SOLVES_IT project. It provides you a very rough idea of what is the project "ATG9_SOLVES_IT" about.

quantitative    resident    expansion    manipulation    ampk    secretory    microscopy    conserved    initiating    infection    regulate    reconstitution    proteome    proximity    optogenetic    cargo    uncover    tomography    atg9    spectrometry    amino    tested    lipids    vesicles    functions    expand    nucleation    components    deregulation    starvation    resolution    reconstituted    molecular    initiation    light    initiates    functional    homeostasis    correlative    biochemically    form    master    autophagosome    atg    em    assayed    implicated    modulate    autophagosomes    vesicle    rapid    lastly    recruitment    membrane    interact    mass    proteins    endocytic    protein    neurodegeneration    cancer    traffics    membranes    torc1    vitro    lysosomal    mediated    dissection    composition    energy    transmembrane    occurs    translational    disease    therapies    tools    biotinylation    function    unknown    nucleate    electron    regulators    aging    cell    identification    crucially    employ    property    acutely    signaling    autophagy    generation    selective    immunity    acid    survival    accessory    cryo   

Project "ATG9_SOLVES_IT" data sheet

The following table provides information about the project.

Coordinator
THE FRANCIS CRICK INSTITUTE LIMITED 

Organization address
address: 1 MIDLAND ROAD
city: LONDON
postcode: NW1 1AT
website: www.crick.ac.uk

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country United Kingdom [UK]
 Total cost 2˙121˙055 €
 EC max contribution 2˙121˙055 € (100%)
 Programme 1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC))
 Code Call ERC-2017-ADG
 Funding Scheme ERC-ADG
 Starting year 2018
 Duration (year-month-day) from 2018-07-01   to  2023-06-30

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    THE FRANCIS CRICK INSTITUTE LIMITED UK (LONDON) coordinator 2˙121˙055.00

Map

 Project objective

Autophagy is a conserved, lysosomal-mediated pathway required for cell homeostasis and survival. It is controlled by the master regulators of energy (AMPK) and growth (TORC1) and mediated by the ATG (autophagy) proteins. Deregulation of autophagy is implicated in cancer, immunity, infection, aging and neurodegeneration. Autophagosomes form and expand using membranes from the secretory and endocytic pathways but how this occurs is not understood. ATG9, the only transmembrane ATG protein traffics through the cell in vesicles, and is essential for rapid initiation and expansion of the membranes which form the autophagosome. Crucially, how ATG9 functions is unknown. I will determine how ATG9 initiates the formation and expansion of the autophagosome by amino acid starvation through a molecular dissection of proteins resident in ATG9 vesicles which modulate the composition and property of the initiating membrane. I will employ high resolution light and electron microscopy to characterize the nucleation of the autophagosome, proximity-specific biotinylation and quantitative Mass Spectrometry to uncover the proteome required for the function of the ATG9, and optogenetic tools to acutely regulate signaling lipids. Lastly, with our tools and knowledge I will develop an in vitro reconstitution system to define at a molecular level how ATG9 vesicle proteins, membranes that interact with ATG9 vesicles, and other accessory ATG components nucleate and form an autophagosome. In vitro reconstitution of autophagosomes will be assayed biochemically, and by correlative light and cryo-EM and cryo-EM tomography, while functional reconstitution of autophagy will be tested by selective cargo recruitment. The development of a reconstituted system and identification proteins and lipids which are key components for autophagosome formation will provide a means to identify a new generation of targets for translational work leading to manipulation of autophagy for disease related therapies.

Are you the coordinator (or a participant) of this project? Plaese send me more information about the "ATG9_SOLVES_IT" project.

For instance: the website url (it has not provided by EU-opendata yet), the logo, a more detailed description of the project (in plain text as a rtf file or a word file), some pictures (as picture files, not embedded into any word file), twitter account, linkedin page, etc.

Send me an  email (fabio@fabiodisconzi.com) and I put them in your project's page as son as possible.

Thanks. And then put a link of this page into your project's website.

The information about "ATG9_SOLVES_IT" are provided by the European Opendata Portal: CORDIS opendata.

More projects from the same programme (H2020-EU.1.1.)

evolSingleCellGRN (2019)

Constraint, Adaptation, and Heterogeneity: Genomic and single-cell approaches to understanding the evolution of developmental gene regulatory networks

Read More  

IMMUNOTHROMBOSIS (2019)

Cross-talk between platelets and immunity - implications for host homeostasis and defense

Read More  

HEIST (2020)

High-temperature Electrochemical Impedance Spectroscopy Transmission electron microscopy on energy materials

Read More