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Reconstitution and Structural Analysis of a Minimal Mechanosensitive Focal Adhesion Complex

Total Cost €


EC-Contrib. €






 FA x Force project word cloud

Explore the words cloud of the FA x Force project. It provides you a very rough idea of what is the project "FA x Force" about.

recruitment    view    structural    manner    regulating    tool    cytoskeleton    multidisciplinary    components    functional    vinculin    experiments    solving    innovative    structure    actin    transduction    assembly    filaments    obtain    fas    deformation    signaling    conformational    actomyosin    assemble    biomedical    cryo    light    regulation    effect    made    resolution    direct    questions    core    biochemically    triggered    receptor    microscopy    image    yielding    complexes    wise    extracellular    interactions    unknown    integrin    molecular    therapeutic    outside    macromolecular    ecm    dynamic    impacts    individual    benefit    receptors    ideal    talin    regulate    couple    protein    reconstruction    dependent    matrix    maturation    augmented    mechanisms    largely    observation    proteins    environment    interact    focal    fa    though    hierarchal    em    reconstructions    structurally    force    ligands    adhesions    generating    connect    alone    network    cells    cytoplasmic    carry    inside    vivo    activation   

Project "FA x Force" data sheet

The following table provides information about the project.


Organization address
city: Munich
postcode: 80539

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Germany [DE]
 Total cost 159˙460 €
 EC max contribution 159˙460 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2017
 Funding Scheme MSCA-IF-EF-ST
 Starting year 2019
 Duration (year-month-day) from 2019-09-01   to  2021-08-31


Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 


 Project objective

Focal adhesions (FAs) are receptor complexes that couple the extracellular matrix (ECM) to the actin cytoskeleton, allowing inside-out and outside-in signaling between cells and their environment. FA formation is triggered by activation of integrin receptors, which interact with cytoplasmic ligands to connect with the actin cytoskeleton and regulate numerous signaling pathways. FA maturation requires force transduction between the actin cytoskeleton and ECM, which results in protein recruitment, likely through conformational changes and force-dependent protein deformation. The molecular mechanisms regulating this process are largely unknown, though many of the key components have been identified and are well characterized in vivo. I will carry out a hierarchal reconstruction of the core FA components, namely talin, vinculin, and integrin, both alone and in the presence of actin filaments or a force-generating actomyosin network. This will allow direct observation of (1) how core components of FAs assemble and regulate each other, (2) how actin impacts the formation and structure of this core complex, and (3) the effect of force on the assembly, overall complex structure, and conformational states of individual proteins. Interactions between key proteins will be analyzed both biochemically and structurally using cryo-EM in a step-wise manner to obtain a comprehensive image of FA assembly. Recent technical advances have made cryo-EM an ideal tool for solving high-resolution reconstructions of macromolecular complexes like FAs. Structural approaches will be augmented by light microscopy experiments, to provide a dynamic view of complex assembly and regulation. By using multidisciplinary, innovative approaches, this project will address both functional and structural questions about FA assembly, yielding insight into key interactions and therapeutic targets that will directly benefit biomedical research in this field.

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