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FA x Force SIGNED

Reconstitution and Structural Analysis of a Minimal Mechanosensitive Focal Adhesion Complex

Total Cost €

0

EC-Contrib. €

0

Partnership

0

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 FA x Force project word cloud

Explore the words cloud of the FA x Force project. It provides you a very rough idea of what is the project "FA x Force" about.

assemble    functional    receptor    benefit    ecm    unknown    experiments    matrix    talin    regulation    cryo    solving    force    receptors    components    environment    multidisciplinary    cytoplasmic    therapeutic    ideal    made    filaments    proteins    carry    activation    effect    extracellular    protein    microscopy    image    observation    focal    structural    mechanisms    obtain    fa    largely    view    though    regulating    integrin    recruitment    reconstructions    tool    innovative    couple    resolution    dependent    core    yielding    deformation    outside    questions    impacts    cytoskeleton    maturation    triggered    complexes    cells    biochemically    network    manner    ligands    augmented    individual    actin    reconstruction    wise    inside    vinculin    light    alone    fas    regulate    direct    adhesions    dynamic    conformational    hierarchal    biomedical    signaling    actomyosin    interact    interactions    assembly    em    transduction    macromolecular    connect    generating    molecular    structure    vivo    structurally   

Project "FA x Force" data sheet

The following table provides information about the project.

Coordinator
MAX-PLANCK-GESELLSCHAFT ZUR FORDERUNG DER WISSENSCHAFTEN EV 

Organization address
address: HOFGARTENSTRASSE 8
city: Munich
postcode: 80539
website: www.mpg.de

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Germany [DE]
 Total cost 159˙460 €
 EC max contribution 159˙460 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2017
 Funding Scheme MSCA-IF-EF-ST
 Starting year 2019
 Duration (year-month-day) from 2019-09-01   to  2021-08-31

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    MAX-PLANCK-GESELLSCHAFT ZUR FORDERUNG DER WISSENSCHAFTEN EV DE (Munich) coordinator 159˙460.00

Map

 Project objective

Focal adhesions (FAs) are receptor complexes that couple the extracellular matrix (ECM) to the actin cytoskeleton, allowing inside-out and outside-in signaling between cells and their environment. FA formation is triggered by activation of integrin receptors, which interact with cytoplasmic ligands to connect with the actin cytoskeleton and regulate numerous signaling pathways. FA maturation requires force transduction between the actin cytoskeleton and ECM, which results in protein recruitment, likely through conformational changes and force-dependent protein deformation. The molecular mechanisms regulating this process are largely unknown, though many of the key components have been identified and are well characterized in vivo. I will carry out a hierarchal reconstruction of the core FA components, namely talin, vinculin, and integrin, both alone and in the presence of actin filaments or a force-generating actomyosin network. This will allow direct observation of (1) how core components of FAs assemble and regulate each other, (2) how actin impacts the formation and structure of this core complex, and (3) the effect of force on the assembly, overall complex structure, and conformational states of individual proteins. Interactions between key proteins will be analyzed both biochemically and structurally using cryo-EM in a step-wise manner to obtain a comprehensive image of FA assembly. Recent technical advances have made cryo-EM an ideal tool for solving high-resolution reconstructions of macromolecular complexes like FAs. Structural approaches will be augmented by light microscopy experiments, to provide a dynamic view of complex assembly and regulation. By using multidisciplinary, innovative approaches, this project will address both functional and structural questions about FA assembly, yielding insight into key interactions and therapeutic targets that will directly benefit biomedical research in this field.

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