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FA x Force SIGNED

Reconstitution and Structural Analysis of a Minimal Mechanosensitive Focal Adhesion Complex

Total Cost €

0

EC-Contrib. €

0

Partnership

0

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 FA x Force project word cloud

Explore the words cloud of the FA x Force project. It provides you a very rough idea of what is the project "FA x Force" about.

ecm    reconstructions    cells    biomedical    individual    alone    structure    therapeutic    light    connect    integrin    largely    proteins    core    assemble    dynamic    direct    impacts    network    cytoskeleton    structural    conformational    unknown    regulating    receptor    triggered    reconstruction    effect    environment    solving    force    talin    complexes    vivo    interact    outside    yielding    recruitment    ligands    transduction    deformation    made    dependent    vinculin    though    questions    couple    activation    obtain    adhesions    view    augmented    inside    biochemically    em    protein    ideal    cryo    extracellular    actomyosin    interactions    cytoplasmic    resolution    filaments    microscopy    innovative    regulate    wise    multidisciplinary    carry    image    observation    focal    mechanisms    structurally    manner    fa    regulation    receptors    functional    molecular    matrix    components    benefit    fas    signaling    maturation    experiments    generating    hierarchal    actin    macromolecular    tool    assembly   

Project "FA x Force" data sheet

The following table provides information about the project.

Coordinator
MAX-PLANCK-GESELLSCHAFT ZUR FORDERUNG DER WISSENSCHAFTEN EV 

Organization address
address: HOFGARTENSTRASSE 8
city: Munich
postcode: 80539
website: www.mpg.de

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Germany [DE]
 Total cost 159˙460 €
 EC max contribution 159˙460 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2017
 Funding Scheme MSCA-IF-EF-ST
 Starting year 2019
 Duration (year-month-day) from 2019-09-01   to  2021-08-31

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    MAX-PLANCK-GESELLSCHAFT ZUR FORDERUNG DER WISSENSCHAFTEN EV DE (Munich) coordinator 159˙460.00

Map

 Project objective

Focal adhesions (FAs) are receptor complexes that couple the extracellular matrix (ECM) to the actin cytoskeleton, allowing inside-out and outside-in signaling between cells and their environment. FA formation is triggered by activation of integrin receptors, which interact with cytoplasmic ligands to connect with the actin cytoskeleton and regulate numerous signaling pathways. FA maturation requires force transduction between the actin cytoskeleton and ECM, which results in protein recruitment, likely through conformational changes and force-dependent protein deformation. The molecular mechanisms regulating this process are largely unknown, though many of the key components have been identified and are well characterized in vivo. I will carry out a hierarchal reconstruction of the core FA components, namely talin, vinculin, and integrin, both alone and in the presence of actin filaments or a force-generating actomyosin network. This will allow direct observation of (1) how core components of FAs assemble and regulate each other, (2) how actin impacts the formation and structure of this core complex, and (3) the effect of force on the assembly, overall complex structure, and conformational states of individual proteins. Interactions between key proteins will be analyzed both biochemically and structurally using cryo-EM in a step-wise manner to obtain a comprehensive image of FA assembly. Recent technical advances have made cryo-EM an ideal tool for solving high-resolution reconstructions of macromolecular complexes like FAs. Structural approaches will be augmented by light microscopy experiments, to provide a dynamic view of complex assembly and regulation. By using multidisciplinary, innovative approaches, this project will address both functional and structural questions about FA assembly, yielding insight into key interactions and therapeutic targets that will directly benefit biomedical research in this field.

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