Opendata, web and dolomites


Chromosome Replication Visualised by Cryo-EM

Total Cost €


EC-Contrib. €






 CRYOREP project word cloud

Explore the words cloud of the CRYOREP project. It provides you a very rough idea of what is the project "CRYOREP" about.

dictating    compact    genome    revealed    stability    substrate    firing    simplified    strategies    duplication    cryo    motor    dna    perform    polymerases    opens    causing    think    isolated    recruited    generate    structural    imaging    catalysed    argue    linked    understand    regulatory    movie    encircles    origin    arrays    events    eject    entire    initiation    investigations    chromosome    duplex    replicative    artificially    duplicated    resolution    linear    instability    plays    onset    start    purified    cell    cells    genomic    formed    activators    mechanisms    natural    disease    unwinding    sites    helicase    reconstitution    chromatin    replication    achievements    performed    cellular    ploidy    ring    roles    nucleosome    untwisting    regulate    tightly    double    errors    molecular    inactive    frozen    eukaryotic    helix    protocols    once    electron    image    basis    site    proteins    regulated    experiments    microscope    strand    forks    abnormalities    fork    propagation    templates    first    cancer    visual    genetic    loading    biochemistry    mcm    reaction    stimulating    cycle    progression    machinery    interaction   

Project "CRYOREP" data sheet

The following table provides information about the project.


Organization address
address: 1 MIDLAND ROAD
city: LONDON
postcode: NW1 1AT

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country United Kingdom [UK]
 Total cost 2˙000˙000 €
 EC max contribution 2˙000˙000 € (100%)
 Programme 1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC))
 Code Call ERC-2018-COG
 Funding Scheme ERC-COG
 Starting year 2019
 Duration (year-month-day) from 2019-03-01   to  2024-02-29


Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 


 Project objective

In eukaryotic cells, DNA replication is tightly regulated to ensure that the genome is duplicated only once per cell cycle. Errors in the control mechanisms that regulate chromosome ploidy cause genomic instability, which is linked to the development of cellular abnormalities, genetic disease and the onset of cancer. Recent reconstitution experiments performed with purified proteins revealed that initiation of eukaryotic genome duplication requires three distinct steps. First, DNA replication start sites are identified and targeted for the loading of an inactive MCM helicase motor, which encircles the double helix. Second, MCM activators are recruited, causing duplex-DNA untwisting. Third, upon interaction with a firing factor, the MCM ring opens to eject one DNA strand, leading to unwinding of the replication fork and duplication by dedicated replicative polymerases. These three events are not understood at a molecular level. Structural investigations so far aimed at imaging artificially isolated replication steps and used simplified templates, such as linear duplex DNA to study helicase loading or pre-formed forks to understand unwinding. However, the natural substrate of the eukaryotic replication machinery is not DNA but rather chromatin, formed of nucleosome arrays that compact the genome. Chromatin plays important regulatory roles in all steps of DNA replication, by dictating origin start-site selection and stimulating replication fork progression. Only by studying chromatin replication, we argue, will we understand the molecular basis of genome propagation. To this end, we have developed new protocols to perform visual biochemistry experiments under the cryo-electron microscope, to image chromatin duplication at high resolution, frozen as it is being catalysed. Using these strategies we want to generate a molecular movie of the entire replication reaction. Our achievements will change the way we think about genome stability in eukaryotic cells.

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The information about "CRYOREP" are provided by the European Opendata Portal: CORDIS opendata.

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