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CLOCK SIGNED

Characterization of the circadian chromatin landscape using a novel CRISPR/Cas9-guided proximity-labelling technique

Total Cost €

0

EC-Contrib. €

0

Partnership

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 CLOCK project word cloud

Explore the words cloud of the CLOCK project. It provides you a very rough idea of what is the project "CLOCK" about.

shown    generation    function    clock    mechanism    shift    rre    caspex    environmental    quantitative    clocks    multiple    humans    first    promoter    rnai    rhythmic    locus    proteomics    rres    pathologies    union    lag    strategies    jet    regulatory    prevalent    regulation    few    regulators    proteins    possess    synchrony    organisms    located    drive    oscillator    dysregulation    oscillators    mammalian    24    physiological    disorders    cis    cas9    apex    crispr    treatment    obesity    enhancer    boxes    mainly    modifiers    metabolic    chromatin    caused    limitations    cycles    left    cells    endogenous    living    regions    characterization    proposes    node    rhythm    subsequent    behavioral    landscape    cancer    cardiovascular    screen    neurodegenerative    transcription    rhythms    unbiased    circadian    gaps    box    diabetes    sequences    diseases    labelling    stay    central    molecular    insights    secondary    health    protein    dynamics    genomic    binding    region    tight    functions   

Project "CLOCK" data sheet

The following table provides information about the project.

Coordinator
CHARITE - UNIVERSITAETSMEDIZIN BERLIN 

Organization address
address: Chariteplatz 1
city: BERLIN
postcode: 10117
website: www.charite.de

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Germany [DE]
 Total cost 162˙806 €
 EC max contribution 162˙806 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2018
 Funding Scheme MSCA-IF-EF-ST
 Starting year 2020
 Duration (year-month-day) from 2020-09-01   to  2022-08-31

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    CHARITE - UNIVERSITAETSMEDIZIN BERLIN DE (BERLIN) coordinator 162˙806.00

Map

 Project objective

To stay in synchrony with environmental cycles, most living organisms possess endogenous clocks. Circadian clocks are molecular oscillators present in most mammalian cells that drive circadian (~24 h) rhythms of a wide range of molecular, physiological and behavioral functions. Circadian clocks are essential for health. In humans their dysregulation (e.g. caused by shift work, jet lag etc.) has been associated with the development of multiple pathologies (e.g. cancer, metabolic diseases like diabetes and obesity as well as cardiovascular and neurodegenerative diseases) prevalent in the European Union.

One central aspect of molecular oscillator function is the tight regulation of circadian transcription. Over the years, several proteins and cis-regulatory enhancer elements (i.e. specific sequences located around the promoter region, e.g. E-box, RRE, D-box) have been shown to be essential for circadian transcription. However, mainly because of technical limitations, those studies focused on few regulators and have left many gaps in the understanding of the dynamics of the circadian transcription. Therefore, this project proposes to use state-of-the-art quantitative genomic-locus proteomics to provide the first comprehensive and unbiased characterization of the rhythmic protein binding at key circadian regulatory regions – a key regulatory node of circadian clock function Using a CRISPR/Cas9-APEX labelling method (CASPEX), we will first characterize the circadian chromatin landscape of the three main circadian regulatory regions (i.e. E-boxes, RREs, D-boxes). We expect to find new clock modifiers, whose role for circadian rhythm generation will be investigated in a subsequent part of the project, using an RNAi-based secondary screen. Overall, this project will provide novel insights in the circadian oscillator mechanism in humans, which is essential for developing better treatment strategies for circadian clock-associated disorders.

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The information about "CLOCK" are provided by the European Opendata Portal: CORDIS opendata.

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