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CLOCK SIGNED

Characterization of the circadian chromatin landscape using a novel CRISPR/Cas9-guided proximity-labelling technique

Total Cost €

0

EC-Contrib. €

0

Partnership

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 CLOCK project word cloud

Explore the words cloud of the CLOCK project. It provides you a very rough idea of what is the project "CLOCK" about.

proteins    central    labelling    rnai    disorders    unbiased    limitations    multiple    mammalian    insights    endogenous    generation    union    living    oscillator    strategies    possess    organisms    clock    cis    regulators    diseases    shown    shift    mainly    circadian    stay    behavioral    metabolic    chromatin    boxes    gaps    secondary    node    characterization    landscape    proposes    crispr    health    molecular    binding    pathologies    obesity    first    cardiovascular    box    modifiers    caused    cells    rres    proteomics    left    transcription    region    drive    clocks    promoter    caspex    located    cancer    tight    sequences    quantitative    rhythms    physiological    function    24    regions    screen    functions    oscillators    environmental    protein    dynamics    prevalent    synchrony    rhythm    cas9    humans    few    rre    regulation    dysregulation    diabetes    cycles    neurodegenerative    enhancer    apex    mechanism    treatment    rhythmic    locus    subsequent    lag    genomic    regulatory    jet   

Project "CLOCK" data sheet

The following table provides information about the project.

Coordinator
CHARITE - UNIVERSITAETSMEDIZIN BERLIN 

Organization address
address: Chariteplatz 1
city: BERLIN
postcode: 10117
website: www.charite.de

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Germany [DE]
 Total cost 162˙806 €
 EC max contribution 162˙806 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2018
 Funding Scheme MSCA-IF-EF-ST
 Starting year 2020
 Duration (year-month-day) from 2020-09-01   to  2022-08-31

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    CHARITE - UNIVERSITAETSMEDIZIN BERLIN DE (BERLIN) coordinator 162˙806.00

Map

 Project objective

To stay in synchrony with environmental cycles, most living organisms possess endogenous clocks. Circadian clocks are molecular oscillators present in most mammalian cells that drive circadian (~24 h) rhythms of a wide range of molecular, physiological and behavioral functions. Circadian clocks are essential for health. In humans their dysregulation (e.g. caused by shift work, jet lag etc.) has been associated with the development of multiple pathologies (e.g. cancer, metabolic diseases like diabetes and obesity as well as cardiovascular and neurodegenerative diseases) prevalent in the European Union.

One central aspect of molecular oscillator function is the tight regulation of circadian transcription. Over the years, several proteins and cis-regulatory enhancer elements (i.e. specific sequences located around the promoter region, e.g. E-box, RRE, D-box) have been shown to be essential for circadian transcription. However, mainly because of technical limitations, those studies focused on few regulators and have left many gaps in the understanding of the dynamics of the circadian transcription. Therefore, this project proposes to use state-of-the-art quantitative genomic-locus proteomics to provide the first comprehensive and unbiased characterization of the rhythmic protein binding at key circadian regulatory regions – a key regulatory node of circadian clock function Using a CRISPR/Cas9-APEX labelling method (CASPEX), we will first characterize the circadian chromatin landscape of the three main circadian regulatory regions (i.e. E-boxes, RREs, D-boxes). We expect to find new clock modifiers, whose role for circadian rhythm generation will be investigated in a subsequent part of the project, using an RNAi-based secondary screen. Overall, this project will provide novel insights in the circadian oscillator mechanism in humans, which is essential for developing better treatment strategies for circadian clock-associated disorders.

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The information about "CLOCK" are provided by the European Opendata Portal: CORDIS opendata.

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