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CLOCK SIGNED

Characterization of the circadian chromatin landscape using a novel CRISPR/Cas9-guided proximity-labelling technique

Total Cost €

0

EC-Contrib. €

0

Partnership

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 CLOCK project word cloud

Explore the words cloud of the CLOCK project. It provides you a very rough idea of what is the project "CLOCK" about.

regulation    cardiovascular    first    strategies    dynamics    living    oscillator    proteins    central    diseases    mechanism    health    gaps    cells    metabolic    treatment    generation    rhythmic    promoter    enhancer    shown    regulatory    locus    jet    characterization    chromatin    regions    pathologies    lag    physiological    endogenous    crispr    functions    24    insights    secondary    circadian    rre    landscape    proteomics    rres    obesity    apex    quantitative    genomic    transcription    unbiased    environmental    molecular    humans    region    labelling    drive    rhythm    mammalian    cycles    shift    union    stay    limitations    left    function    cancer    organisms    possess    synchrony    box    caspex    multiple    dysregulation    prevalent    proposes    neurodegenerative    diabetes    behavioral    rnai    cis    oscillators    cas9    regulators    rhythms    tight    node    clocks    disorders    modifiers    located    subsequent    mainly    binding    few    screen    sequences    caused    clock    protein    boxes   

Project "CLOCK" data sheet

The following table provides information about the project.

Coordinator
CHARITE - UNIVERSITAETSMEDIZIN BERLIN 

Organization address
address: Chariteplatz 1
city: BERLIN
postcode: 10117
website: www.charite.de

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Germany [DE]
 Total cost 162˙806 €
 EC max contribution 162˙806 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2018
 Funding Scheme MSCA-IF-EF-ST
 Starting year 2020
 Duration (year-month-day) from 2020-09-01   to  2022-08-31

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    CHARITE - UNIVERSITAETSMEDIZIN BERLIN DE (BERLIN) coordinator 162˙806.00

Map

 Project objective

To stay in synchrony with environmental cycles, most living organisms possess endogenous clocks. Circadian clocks are molecular oscillators present in most mammalian cells that drive circadian (~24 h) rhythms of a wide range of molecular, physiological and behavioral functions. Circadian clocks are essential for health. In humans their dysregulation (e.g. caused by shift work, jet lag etc.) has been associated with the development of multiple pathologies (e.g. cancer, metabolic diseases like diabetes and obesity as well as cardiovascular and neurodegenerative diseases) prevalent in the European Union.

One central aspect of molecular oscillator function is the tight regulation of circadian transcription. Over the years, several proteins and cis-regulatory enhancer elements (i.e. specific sequences located around the promoter region, e.g. E-box, RRE, D-box) have been shown to be essential for circadian transcription. However, mainly because of technical limitations, those studies focused on few regulators and have left many gaps in the understanding of the dynamics of the circadian transcription. Therefore, this project proposes to use state-of-the-art quantitative genomic-locus proteomics to provide the first comprehensive and unbiased characterization of the rhythmic protein binding at key circadian regulatory regions – a key regulatory node of circadian clock function Using a CRISPR/Cas9-APEX labelling method (CASPEX), we will first characterize the circadian chromatin landscape of the three main circadian regulatory regions (i.e. E-boxes, RREs, D-boxes). We expect to find new clock modifiers, whose role for circadian rhythm generation will be investigated in a subsequent part of the project, using an RNAi-based secondary screen. Overall, this project will provide novel insights in the circadian oscillator mechanism in humans, which is essential for developing better treatment strategies for circadian clock-associated disorders.

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The information about "CLOCK" are provided by the European Opendata Portal: CORDIS opendata.

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